FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13200
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6584.984848484848485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390.29545454545454547No Hit
ATGATGATCTCACTATCCCCAGAGCTGCTATCAATAAAATGATCAAAGAGACTCTTCCTAATGTCCGGGTG260.196969696969697No Hit
TCCCTACACGACGCGGGAGGACAATGTTACAGTGTAAGAAACTCCATATCCATATGTTCCCATTCGCATTT250.1893939393939394No Hit
GCCCCTGTGGCTGCTGCCACCACAGCTGCTCCTGCTGCTGCTGCAGCCCCAGCTAAGGTTGAAGCCAAGGA240.18181818181818182No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTA230.17424242424242423No Hit
TTTGAATAGTTACGATGAGTGCTTGCAAGAAAAAGGAAGCTATCACTTAAAAGTAAAAAACAGTTGTTCAC200.15151515151515152No Hit
TTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAGGGTTCGAGGGCACGAGTTCGA200.15151515151515152No Hit
GATATTTCTGATGAAAACAAGAAGAAAGCAAAAATTCAAAAGAAACCAATTAAGAAAAAGAAAGAGGATGA200.15151515151515152No Hit
GTTCAGAAGTCGCTGTCCTTACTACTTTTGCGGAAGTATGGAAGTCACAACTACACAGAGATTTCTCAGCC200.15151515151515152No Hit
CTATTATTTCATTGGTTGGGATGCTTTGCCAGGTCATGTGCTTATTGTCTCATTTTGTAGTCTTTTAAAGT190.14393939393939392No Hit
ATGAAATATTCTCCCAGGAAAATCTAGAAGATAATGATGTCTCAGACATTTTAAAATTACTAGCATTACAG190.14393939393939392No Hit
GTGTGTAAGTTTGATAAATTCTAACCTTTTCTAATAGATTTGTGTATTTTATGGTAGTGAATGATAAAATA190.14393939393939392No Hit
CAGCTAAATAGGTTGTTGTTGATTTGGTTAAAAAATAGTAGAGGGATGATGATTAATAAGAGGGATGACAT190.14393939393939392No Hit
GTACTGTAAAATAGAATTTCACATGAATTGCTGGAAGAAGTTAAAAACTACAACCTTTAATGATAAAATTG190.14393939393939392No Hit
GGCGAAGCTGGCCGAGCAGGCTGAGCGATACGACGAAATGGTGGAGTCAATGAAGAAAGTAGCAGGGATGG180.13636363636363638No Hit
ATGGAATATAAGGAACAATTATGGGATGTCATCAGAATGGATGCCATAGGACCTACAGCTCCCTTTCTCTT180.13636363636363638No Hit
GGCCCATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTCCGAGGCCAGCACACCAAGACCACTGGCCGCC170.12878787878787878No Hit
TCCCTACACGACGCGGGGGCCCAACATGGCGGCGGCCGTAAGGTGCATGGGTAGAGCCTTGATACATCATC170.12878787878787878No Hit
GCTCTGAGGCCTCCCCCATTGGGAAGTCTCCATATACACTAGACAGCCTGTATTGGAGCGTCAAGCCAGCC170.12878787878787878No Hit
CCCCTAATGAAGTGCATTAGTCAAGAGCTCAGTGACCTCTGACAAGGCTCAGTATGGGATTATAGCCTGAC170.12878787878787878No Hit
CTGCTCTGGTGCCTGGTGGTGCTAGCTCCTCCCACCTCAGCACCTGCTGCATCTGGAGCAGCCCAAGCCTC160.12121212121212122No Hit
CCCTGGGAGACAGTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCATAGCCGTGGGGTGACTTCCCTG160.12121212121212122No Hit
GCTTTAAAGAAGTCAAGTAAGGGAGAATTGACTACGCTTATACATCAGCTTCAAGAAAAGGACAAGTTACT160.12121212121212122No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG160.12121212121212122No Hit
CTTATAATAGTGGCAAATAAAAAGTCCTATTTGTGAGAAAAATACGTGCGCTTTTATGGAGCTAGACAAGT160.12121212121212122No Hit
GGATTAAAAAACAAATCCAGGGAATACAGTTTACAGCTGATACATTCAAAATATAAAAACAGATAAAATAT160.12121212121212122No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAA160.12121212121212122No Hit
GTACAAAAGCTTATCCCTGTAACACATGTGCCATAATATACAAACTTCTACTTTCGTCAGTCCTTAACATC160.12121212121212122No Hit
TTATCAAACTGGTTTCAAAGCACAGAGCTCAAGTAATTTACACCAGAAATACCAAGGGTGGAGATGCTCCA160.12121212121212122No Hit
GCGTATGGCCACAGAAGTTGCTGCTGACGCTCTGGGTGAAGAATGGAAGGGTTATGTGGTCCGAATCAGTG160.12121212121212122No Hit
GTTCCAGAAACAGACCCTGTTAGTCCTTCTACTTACCTGCTGGATTTTTCAAGCACTAAATTTATAACTTT150.11363636363636363No Hit
ATGCTCAGATCGACTGGCATGATTTTGTGGTGGTGGAAACAGTGGACTTCCAACCCAATGAGCAAGGGAAC150.11363636363636363No Hit
GGGTCACAGACTGGGATGAGCAACGGGCTGAAGGCACGTTTCCCGGGAAGATCTGAACTGGCTGCATCTCC150.11363636363636363No Hit
GTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAAGAGAATTAATGTGCGTATTGAGCACATTAAGCA150.11363636363636363No Hit
TAGTAAAGGTGAAGAATCTAGATGGCAAACCTGTGTATAGCCACTCAGATCCTTCTGAGGTCGCAGTGCAG150.11363636363636363No Hit
GTGTGGCAGCGTGCACCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAACTGCTTGAATCCGGCAG150.11363636363636363No Hit
TGGTGAAGGCAGTAGTTCTGGAAAAGTCACTGGGGACGAGACAGGTGCTAAAGTTGAACGAGCTGATGGAT150.11363636363636363No Hit
CTTTGAAAGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCG150.11363636363636363No Hit
AATGTGTACAGGGTCCTTTTGCAATAAAACTGGTTATGACTTGATCCAAGTGTTTAACAATTGGGGCTGTT150.11363636363636363No Hit
GGGCTTGTTTTCCAAAGAAAAGTATTGTTTGGAGGAGCAAAGTTAAAAGCCTACCTAAGCATATCGTAAAG150.11363636363636363No Hit
ATATTGTACTGATCAGCAGCTCTCATTTTAACATCAGTTAGGCATAATATTTCATAGTTTTCAAGCCTTTT140.10606060606060608No Hit
GTGCAGACTCACCCTAAAGGGCGGCCCAGGCCCCACGCTAGAAGGCTGGCGAGACCGAAGGCAGCATGTGA140.10606060606060608No Hit
ACTTAGGAGGCTGAGGTGGCAGAATTGTTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCGAGATCACACC140.10606060606060608No Hit
ATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTCCGAGGCCAGCACACCAAGACCACTGGCCGCCGTGGC140.10606060606060608No Hit
CCCTACAGGACTGCTGAACAGAGAGCCCAGTGTGACTAGGGATCCTGAGTTTTCTGGGACAATTCCAGCTT140.10606060606060608No Hit
GTAGACATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAG140.10606060606060608No Hit
CTTCCAGAGCAACCTGGTGCCCGCTGACCCTGAGTGAAGGCCGCCTGCCGGGGACTCAGACACTCAGGGAA140.10606060606060608No Hit
ACCCAAGACTCTGAACCTTCCAAGGGACAGGCAGTTCTTCTAAGAAGGGCTCCCCTGTGTGTGAGCAAGAC140.10606060606060608No Hit
AGCAGATGTGGTACCTGTGACTTTAGCACCTAAGAAGTTGGATGGAGCAAGCCACACAAAACCAGGTGAAA140.10606060606060608No Hit
GAAGAGCTCCCGCACATCCATGCCTTTGAACAGAAAACCCTGACCCCAGACCAGTGGGCACGTGAGCGACA140.10606060606060608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)