FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38218.104265402843602No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301.4218009478672986No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG271.2796208530805688No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT120.5687203791469194No Hit
GTATTGATGTCTTTATTCCATTTTGAGTTTAGATTGAGAATATTTTTATTTTCTGAAGGCAGAGATATCTA120.5687203791469194No Hit
GTTGTAATTGCATTTTTTCCCTTTTCTTGGCACCCCTTTAGAAATGTACTGAAGTCTCATTTTTCACCCAT110.5213270142180095No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA90.42654028436018954No Hit
TTGATACTATTGCCTCCCAATTGTGTTTGATATCAAACTTAAGTGAGATCAGAGTGGGAAAAAAAAAATGG90.42654028436018954No Hit
GAAACAGGATAGGAGTTTAGAGAAGGCACCAAAGCTTTCACTTTGGTTTGGCACCAGTTTCTAACCATCTG90.42654028436018954No Hit
CTGTGGCCTTGAACTCTTGCGCTTAAGCCATCCTCTTGTCTCAGCCACACATGTAGCTGAGACGACAGTTG90.42654028436018954No Hit
GTACCACAGCAGTTACATATTCTGGTACGTCTTATTCAGGTTATGAAGCAGCAGTGTATTCAGCTGCATCT90.42654028436018954No Hit
GCTGGTGTGTTTCCAGATGGTTCTTCTAACCAAACTAATTTTTCACTGTTGACAAGCGAGGCAAGGGTTGC90.42654028436018954No Hit
AACCTTTTGTAATGCCATTTTCTAAACTTATTTCTGAGTGTAGTCTCAGCTTAAAGTTGTGTAATACTAAA90.42654028436018954No Hit
GGGTGTTTATGTCATACGTAAAAATAAAATGTGTGACAGTAACAGGACAGGAAAGGCATGAATGGACACAC90.42654028436018954No Hit
GTATTTGTAAAATTGATATGAAAAATAATTTCTCAAAGTCTATTGTTTGATCTAGTTATTTTAATAAAGGA90.42654028436018954No Hit
CATTCAATTGATGGGAGAGTAGTTGAGCCAAAACGTGCTGTAGCAAGAGAGGAATCTGGAAAACCAGGGGC90.42654028436018954No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGG90.42654028436018954No Hit
AGATTCATATTTACTTCTTATACATTTGATAAAGTAAGGCATGGTTGTGGTTAATCTGGTTTATTTTTGTT80.37914691943127965No Hit
GCCTCGGGAAACCACTTCTCAGGCCATGGGATGTCTTTCAATGCACCTTTCAGTGGAGCTCCCAATGGAAA80.37914691943127965No Hit
GTGGTAGGTTAATGTGTTCAGAAGTCGCTGTCCTTACTACTTTTGCGGAAGTATGGAAGTCACAACTACAC80.37914691943127965No Hit
ACTCCTAAATCTTCCCATGCAATTGTTGTAATCTCTTGCTTTCCACAGTTCCACTGGTTCTTCCTTGGGAT80.37914691943127965No Hit
ATTTATATTCCTGTCAATACCCCATTTGGTGTTAAGAATTACCATGAAAATTTTTGCAAGTTTCATAAAGA80.37914691943127965No Hit
GGTCGGATTAACCCATACATGAGCTCTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGT80.37914691943127965No Hit
GGTGTAGAAGTTAAGGACATTGAATTGGTCATGTCACAAGCAAATGTGTCGAGAGCAAAGGCAGTCCGAGC80.37914691943127965No Hit
AACTAAGTATGTGGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.33175355450236965No Hit
GCCTCGGTTTCTATTTATAATGGAGACATGGAAAAAATACTGCTGGACGCACAGCATGAGTCTGGACGGAG70.33175355450236965No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.33175355450236965No Hit
ACTTTGGAGAATGGATTAATACATCTGAGAAGCAGAGACACCACAATGAAGGAAATGATGCATATATCCAA70.33175355450236965No Hit
CCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.33175355450236965No Hit
GTTTAATTAGCTTTCCAACCAACCAAATTTCTGCATTCGAGTCTTAACCATATTTAAGTGTTACTGTGGCT70.33175355450236965No Hit
CCATTGAACCCTCTCACAAAAGTACAAGCTTAAAGGGGCTAAAATCCATTAATACATTCCATTTTATATCC70.33175355450236965No Hit
GGGTGCAGCCGCAGGCTCCAAGCGCCCCACCTGTGTGATAATGGTCAAGCCCCATGAGGAGTACCAGGAGG70.33175355450236965No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGGA60.2843601895734597No Hit
GGCTTGGGTGCGTTCAAGATTCAACTTCACCCGTAACCCACCGCCATGGCCGAGGAAGGCATTGCTGCTGG60.2843601895734597No Hit
GCGGTGGGCAGCCATGAGAACAAAACCTCTTCTGTATTTTTTTTTTCCATTAGTAAAACACAAGACTTCAG60.2843601895734597No Hit
GTTATAGTTTACTCAGCTTCACTGTTACATCCTAGATGAGTATTGTATTCAAAAATACTGGGCCTTAAGTC60.2843601895734597No Hit
GTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAA60.2843601895734597No Hit
CCTTTGACTCTTCTACTGTCTCAGACCTTAGGTAACATACCTGCAGCTGCTTTTCTAACAAACTGTTGATC60.2843601895734597No Hit
GAAATTTGGTACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.2843601895734597No Hit
ATCAAGACCATACTGGTTAACACCGTGAAACCCCATCTCTACCAAAAAAAATACAAAAAATTAGCTGGGTG60.2843601895734597No Hit
AATAATGGTGATAAACTGAGAAAAAGGAGGTCTAAAGTGTATCTATGGGAACTAACAACAGAGATCATTTA60.2843601895734597No Hit
CTGTTGGGGACAGGAGATGCATGGACAGTGTGCTCAAGCTGTGGGCATGTGCTTGCCTGCGGGAGAGGTCC60.2843601895734597No Hit
CATCTTTCCGGTGCAATATCTATCAATTGTGAATCTGGCTGCTGGTGTATAAAAACCTGGATGTAAAGCTG60.2843601895734597No Hit
GACCACGGGTGTTGTAAGCTGGGACACGGAAGCCAAACTGGAATCAAACGCCGACTGTAAATTGTATCTTA60.2843601895734597No Hit
AAGATTTAATGCGATTGCTCTTTCTGCAGCATAGCTTGTCAATAATGGAAACACCAAAAACTGTATTATTT60.2843601895734597No Hit
AATCAACCTGACTGGATTTTTGAATGGAAAAAATGCTCGAGAATTTATGGGAGAACTGTGGCCCCTGCTGC60.2843601895734597No Hit
GTAGACAGAGGCCATAGGTGCAGCTGTGATGGTAGAGGTGGAAGACTGATTTGCATTTTCACCAGGACATG60.2843601895734597No Hit
GGCCGCACTCTTTCTGACTACAACATCCAGAAGGAGTCGACCCTGCACCTGGTCCTGCGGCTGAGGGGTGG60.2843601895734597No Hit
GTTAATGACTTGTTGGGTAGCTATTAAGGTACTAGAGTTGATAAATGTGTACAGGGTCCTTTTGCAATAAA50.23696682464454977No Hit
GCACATAACTATGCACATCCTCCCGTGTACTTTAAATAGTCTCTAAATTACTTCGTAACACCTAATCCAGT50.23696682464454977No Hit
AGTTTTAAGTTATTAGTTTTTAAAATCAGTACTTTTTAATGGAAACAACTTGACCAAAAATTTGTCACAGA50.23696682464454977No Hit
GACTTACTGAAACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.23696682464454977No Hit
GTTTAGGTATGGTTTCGGGTACAATAAGAAAGAGATAAAATGGAATGCTTTATGCCAAACATGTTTATTTT50.23696682464454977No Hit
CAATACCGTAAGGTTGCGTGAACTGTCCTACTGAATCTTCATGGTTGCTTGGATTTTAATCACATCAGAAG50.23696682464454977No Hit
GCACTTTGGGAGACTGAGGTGAGTGGATCATATGAGGAGGCTAGGAGTTCGAGGTCAGCCTGGCCAGCATA50.23696682464454977No Hit
TGTTCCTCCTCCTCTCTCCCCACTTGAACCATGAAGTTTCCAATAATTTTTTTTTCTTTTTTTCCTTCTTT50.23696682464454977No Hit
TCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.23696682464454977No Hit
GCAATGGGCGCAGGCCGTTTGGCATCTCTGCCCTCATCGTGGGTTTCGACTTTGATGGCACTCCTAGGCTC50.23696682464454977No Hit
CTGGTGGGATGAAGGTAAAGGCAGACCGAGATGAATCCTCACCATATGCTGCTATGTTGGCTGCCCAGGAT50.23696682464454977No Hit
CTTTTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.23696682464454977No Hit
ATTTTATATATTGTAGTATCAACATTTTTAAATTAAATGTTTTACATTCACAAAAAAAAAAAAAAAAAAAA40.18957345971563982No Hit
CCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTT40.18957345971563982No Hit
GCTACTCAGGAGGTCAAGGCAGGAGAATTGCTTGAACCCGGGAGGCAGAGGTTACAGTGAGCCGAGATTGT40.18957345971563982No Hit
CAACGTGTTTATAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18957345971563982No Hit
ATCATGTGAGATTTCTTTAAAATGGATTAAACGATTTCTTCAGCCTGAAAAAAAAAAAAAAAAAAAAAAAA40.18957345971563982No Hit
ACTCAGGAGACTGAGACAGGAGAATCGCTTGAACCCGGGAGGCGGAGGCTGCCATGAGCTGAGATCGCGCC40.18957345971563982No Hit
TTTCAGAGAGCCCCAATTCTGCTTTCCCACAAAATAAACCTAATGCCATCAGGCAAAAAAAAAAAAAAAAA40.18957345971563982No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18957345971563982No Hit
CCCTACACGACGCGGGCAATAAAGGCCCACTGGAACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18957345971563982No Hit
CCCACAAAGACAGAACTGATTTTTTTCCCATAATCAGGGGTGAAAAATATACAACTTGTTTCTGAACCAAA40.18957345971563982No Hit
GAGCCACCTGCAAGATGGACACGAGCCACAAGCTGCACTATGAACCTGGGCACTCCGCGCCGATGCCACCG40.18957345971563982No Hit
TCCCTACACGACGCGGGACAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18957345971563982No Hit
GGGCCATGTGCTGCCCTGTTGCCGAGCCCAGCTGTGGGCGGCTCCTGGTGCTAACAACAAAGTTCCACTTC40.18957345971563982No Hit
GGTCAGAGTGATGCAATTGCTGGAGGAAGAGCCATGAGCCGAGGAATGCAGACAGCCTCTTCTCCTCTGGG40.18957345971563982No Hit
GTCTTCACGTTGAGGATTCAACTGGCCAATCACAGGACAGGTGTCCTGGCCTTTCTTCCTGAGGTCTCTAG40.18957345971563982No Hit
CCCCCTGAACAATGCTGGCAGTTGCTACAAATAGATTTATTGGAGGCTTATGGCTCCGGTTCCCCCACAAA40.18957345971563982No Hit
CCTTGGAAAAGGTGTGTGCTGACTTGATAAGAGGCGCAAAAGAAAAGAATCTCAAAGTGAAAGGACCAGTT40.18957345971563982No Hit
GCTAAGTGTCTCTGACAAGCAGTTGACTGACATATTATTGATGGAGAAGAAACAAGAAGCATAAAAGGTAC40.18957345971563982No Hit
TCATAACACTATAATAAACTACATTGTCAAATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18957345971563982No Hit
GATTGTGAGAAAATAAATTTCTGTTGTTAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18957345971563982No Hit
GCTATTAGCCAGGATCATGGTGTAATAAGACATAACGTTTTTCCTTTAAAAAAATTTAAGTGCGTGTGTAG40.18957345971563982No Hit
GACCCAGGGTCCCAGCTGCTGCTTGGGGATGTGCACTGCTGGCCGCCGGCCTTGCAGTCTCTCTACCCTGG40.18957345971563982No Hit
GCTGCGGGAGCTCTTATGGTCTATGATATCACTAGAAGAAGTACATATAACCACTTAAGCAGCTGGTTGAC30.14218009478672985No Hit
GATGAAGATGAGGAAGCTGAGTCAGCTACGGGCAAGCGGGCAGCTGAAGATGATGAGGATGACGATGTCGA30.14218009478672985No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
GCGTCGACTGGCAGATCCGCCAGGTGCGGGCGCTACTGCAGGCACAGCAGGCCTAGGTCCTCCCACACCTG30.14218009478672985No Hit
CTTCTAGCGTGTGGCATCTGACCAATTTTGAATTGGTCCTTTCTATAAAATCAATATTTATAAAAAAAAAA30.14218009478672985No Hit
AACTTATCCCATTGTATTTTTTATAAATAAATATTTTTTTTGAACTTTAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
GTGATCCTCCATCTTAAAGAAGATCAGACAGAGTACCTAGAAGAGAGGCGGGTCAAAGAAGTAGTGAAGAA30.14218009478672985No Hit
CCTTTGAAGATTTACGAGAGAAACTTCTCATGGCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAA30.14218009478672985No Hit
ATCCAAACCATTGGCATCATCGTTTCTTTTGAATTAAAACCAACATATCAGCAATAAAAAAAAAAAAAAAA30.14218009478672985No Hit
CTCTGAAGCCAACTTCTGTATACCTTCACCCATTTCATGAAAATAAAATCAAAAGGGAAATCAAAAATAAA30.14218009478672985No Hit
GAGTTGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
AAACTGATGTTACTAGCCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
GGCATTGTGATTGGAGTAGTGGCCCTGGTTGCTCTGATAGCAGTAGCCCTGGCATGTTTTCTGCATTTCGG30.14218009478672985No Hit
AATTTGAGAGAATCATCTCTAAGCAAAATGTCCTATCTGCAAATGAGAAATTCACAAGCGCACAGGAATTT30.14218009478672985No Hit
CCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
AAATAAATGTGTTTGTGCTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
GTCCTCCTCCACCTTGATGGACCCCTAGTGATGCATCCTGCCTAGGCTGGACTTAACCCCTTCCTCCCTGT30.14218009478672985No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGA30.14218009478672985No Hit
CATTTTCCACTCCAACTTTGTTGACTGTTCTACTCTTTCATAATTGTGTGGGCTGTGAGCCTCAGTATAAT30.14218009478672985No Hit
GTATTATACCCACACCCACCCAAGAACAGGGTGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
GGTTCAGGAGGTACCCAAACTGAAGGTATGTGCACTGCGCGTGACCAGCCGGGCCCGCAGCCGCATCCTCA30.14218009478672985No Hit
TAATTTAGTACTGGGGATCAATGAAAAATTTGATGATACTAAGAATAAAGAAAAGATCCTAAAAGCCTCCA30.14218009478672985No Hit
GGGTTTCTTGGAATTTTATATTTGACAATATTTATACTATACCAAACTCATTTGCAGTTCTTAGGTTTGTT30.14218009478672985No Hit
GTAATAAAGAATTACTTTTCCTTTTGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
CACCCCATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
AAATAAAGATGAATTGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14218009478672985No Hit
CGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAA30.14218009478672985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)