FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483
Sequences flagged as poor quality0
Sequence length71
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52521.14377768828031No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG321.288763592428514No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG190.7652033830044301No Hit
GCTTATTGGACACATGGATTTTCTTCAGATTTGCACTTACTGCTAGCTCTGCTTTTTATGCAGGAGAAAAG150.6041079339508658No Hit
GGGTACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATT140.5638340716874748No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT140.5638340716874748No Hit
GGATCGCTCCTGGTTCCTCGGAACCCTGAGTCCAAACGTCCTAGCTCACCAATAGACTACCCTCCCTCCCA140.5638340716874748No Hit
TTTATAGTTTAGGTAGTATGTTACATTTGAATTTTTGTCTTAAGAAAAGTTGACTGTTCAGATATTTTTCT120.4832863471606927No Hit
GGAAATAAAGCAGAAGGAACGTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.44301248489730166No Hit
GCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.3624647603705195No Hit
CCACTGTGCCTGGCCATCTGCCAGGTTCCAGAACATTTTGATGAAGTAAGGTGCTTGAGTGATGAGAGATT90.3624647603705195No Hit
CCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.3221908981071285No Hit
GTTTTTTCTTATTGCTGTGGAACCTCTTTTGGAGGACGTTAAAGGCGTGTTTTACTTGTTTTTTTAAGAGT80.3221908981071285No Hit
GGGGCAAAAGGAAGACTTGGGTAGTAATCTGGGGGTTCAGATGGGTAGCACTAAGCCAGCTGGCCTAAAGA80.3221908981071285No Hit
GTTCAACACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGCATGGT80.3221908981071285No Hit
CATCTTCATGTGGGCTGGGGTAGAAACTGGTGTCTGCTCTGGTGTGATCTGAAAAGGCGTCTTCACTGCTT80.3221908981071285No Hit
GGTTCCTTCACTGTGCGTGCTGACCATGGTGAGCCCATTGGCAGGGGTACCAAAGTGATCCTCCATCTTAA80.3221908981071285No Hit
GGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTGATGCCTTGGACA80.3221908981071285No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC80.3221908981071285No Hit
GCACCCAGCCAGGCGGTCTCTTTAGAGGATCCTGTACACAGTTCCACTATTAAAACATTTCAGGTTGAATT70.2819170358437374No Hit
GGTTATTTCTTTCTCTTCAGGTCTTTCCTTCTCTCTTTCTTTTTCTTTGTTGTTGTAAAATAATATATTAT70.2819170358437374No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATT70.2819170358437374No Hit
CTTCGAGGGGTTCGTGCTGTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGCAG60.24164317358034634No Hit
TTTCCAGTCATTCAGCTGTGGTTATTTAAAGCAGACTTACATGTAAACCGGAATCCTCTCTATACAAGTTT60.24164317358034634No Hit
CGAGTACGGGGTGTGGCCATGAATCCTGTGGAGCATCCTTTTGGAGGTGGCAACCACCAGCACATCGGCAA60.24164317358034634No Hit
CCAGGTGTCATTAACATCACTATGACCTAAATTCTACATGCCCTAATCCACTAATTTATCAAAATCTCATG60.24164317358034634No Hit
CATCCAGTCTCTCGTGGCGATTGATTATGCTTGTGTGAGGCAATCATGGTGGCATCACCCATAAAGGGAAC60.24164317358034634No Hit
CTGCTGTTAGATTTATGCTTTTCAGAATATGTTCTTAATGAAAGTTTCACCTGACTTGTTATCTAGTCTGA60.24164317358034634No Hit
GAGTGAAACTCCGTCTCAAAAAAAAAAAAAGAAAAAGAAAAAGAAAAAGAAAAGAAATGTTTATAAAACAT60.24164317358034634No Hit
GGGGGAGCTCAGAGTGACCCAAATTCTCAAAGAAAAGTTTCCACGAGCTACAGCTATAAAAGTCACTGACA60.24164317358034634No Hit
CCCTTACCAGCTGGAGCTGCCTGCCCTGCAGTCAGAAGTGCCGAAAGACAGCACACACCAGTGGTTGGACG60.24164317358034634No Hit
GGCTGTCTGGGTTCAAATGCCATCTTTGACACTTGCAAGCTAAATGACATTACTCAAATTAATCGTTCTGC50.20136931131695532No Hit
CTTGAGACCAGGAGGTCAAGATCAGCCTGAGGAATAAAGTAAGCTCCCGTCTCTTAAAAAAAAAGTTTTTA50.20136931131695532No Hit
CAATCAGATGCAAAGCTGAAAGAGATTGTAACAAATTTCTTGGCTGGATTTGAAGCTTAAACTCCTGTGGA50.20136931131695532No Hit
CTGCCATCCCATGTTTCACCAGTCAGACCCCCAGGGCAGGGCAGGGGAAGCAGAATGCAAACTGGGGCACC50.20136931131695532No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA50.20136931131695532No Hit
GTGTTTATGTACTCTTAACAACTCACAGAATAATACATGTTCACTTTATTTTGTAAAATTGGGTTGAGAGG50.20136931131695532No Hit
GTGCTGAGCTCTCACCCTTCCCCCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16109544905356424No Hit
GTTTTTATAAATGTCTTACTCAAGTTCAAACCTCCAGCCTGTGAATCAACTGTGTCTCTTTTTTGACTTGG40.16109544905356424No Hit
CCTGCGGGACATGATCATCCTACCCGAGATGGTGGGCAGCATGGTGGGCGTCTACAACGGCAAGACCTTCA40.16109544905356424No Hit
GCACAACTTTTAGCATTTCAACTACAATGCTTTTCATGGGGAAAGTTCCACATCACCCTCTGGTTACATTT40.16109544905356424No Hit
CTTCCGATCTACCGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16109544905356424No Hit
GCCATGAAGAAGATAGAAGACAACAACACACTTGTGTTCATTGTGGATGTTAAAGCCAACAAGCACCAGAT40.16109544905356424No Hit
ATGAAGTGTATTAAGTAAATAAATAAGTAAATAACCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16109544905356424No Hit
GTAAGGGTTATGTCATTGGAAAATGAGGGGTTGGATTAGAAAGGAAAAAAATGGGTAGGACTTGGACAGAA40.16109544905356424No Hit
GGGTGAGTGTGGCACCCAGAGACTGCTCTTCCTGCCCTCACCCTGCCCCACTCCTACGACTGGGAGCTGAC40.16109544905356424No Hit
CGGGGGCTGGTCCATGGGCGGCTGTGGCGTCAGGATGGAGTCCTAGCTGTGACCTGTGCCCAGGAGGGCGT40.16109544905356424No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA30.12082158679017317No Hit
GGTAACTGTCAAGTCATGATGGAGACTTCAGGTTGTTCTGTATAAAATGCAAAATAAATGTTTTTATTAAC30.12082158679017317No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA30.12082158679017317No Hit
ATGTAGTGCTTAATAAAAATAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
GTAATATTGTAAATACTGGACTCAGGAACTTTTGTTAGGAAAAAATTGAAAGAACTTAAGTCTCGAATGTA30.12082158679017317No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTTGAAATAGGGCCCGTATTTACCCTATA30.12082158679017317No Hit
GCACAAAAAAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
GAGTTGATAAATGTGTACAGGGTCCTTTTGCAATAAAACTGGTTATGACTTGAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
GTTCTAACATATGTAATAATTGCCAGGAGTACAGTGCTCTTGTTGATCTTGTATTCAGTCAGGTTAAAACA30.12082158679017317No Hit
TCCCTACACGACGCGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
TTTACTTTCTGTACAACAAAAAAAGCTTTGTAAATAAAATCTTAACATTTAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
ACATTAGATAATTAAATAGTGAGACATAAATAGAGTGTTTTTCATGGAAAAGCCTTCTTATATCTGAAGAT30.12082158679017317No Hit
ATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGACAACACATAAAAAAAAAAAAAAAA30.12082158679017317No Hit
GCACTGGTGGCCAAGTCCTCCATCCTGCTGGATGTCAAGCCTTGGGATGATGAGACGGACATGGCCCAGCT30.12082158679017317No Hit
AAAAAAAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
GGAGAATTGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATAGCACCACTGCACTCCAGCCTGGG30.12082158679017317No Hit
CTATTGAGCTGTAGACGAACTGTTTCTCTAATTATAAAGCAAACTGTTTGGGATATTCAGGGAAACTACCC30.12082158679017317No Hit
CGCTGCAGCTGCGGGCCGGGCCGCGGGTCAAGCTGCAACTCTGGGACACCGCGGGCCACGAGCGCTTCAGC30.12082158679017317No Hit
CGTTGGTTGGCTGGATTCAGTAATAAATATGTAAGGCCTTTCTTTTTAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
CTTTTACCCCCTTCTTGTCTTTTCCCTTCTATTCTTTACCGTCTGGATATGATTCCTCCATTATTAAACTC30.12082158679017317No Hit
ATAAAACACTAACCATGCATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
GGTGTGGTGGCTGGCACCTGTAATACCAGCTACTAGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGA30.12082158679017317No Hit
TTCTTCTCCCCTGTATTTTCTAACTTTGTACAGTGAATATATAATTATAATTTTACAAATTAAATCTAAAA30.12082158679017317No Hit
GTTTGGATTTTAATTGTGATGCAGAAGTTATAGTAACAAACATTTGGTTTTGTACAGACATTATTTCCACT30.12082158679017317No Hit
CACCAATACAATAAAGGATGAAAGACGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
CCAAGGAGCCGTGTTAGGAGGGGTCGGGGAGAAATCAGGCTCTAGAAGCTCATAAAATTCAACGTGAGGAA30.12082158679017317No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
GCTCACTCACCCGCCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACAC30.12082158679017317No Hit
GTGACTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGTGGATTGCTTGAGCCTAGGAGTTCAA30.12082158679017317No Hit
GGCTTGCATAGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
ATGTTTGGATATATGAAATTACATCCAGAGGATTTTCCTGAAGAAGATAAGAAAACATATGGTGAAATTTT30.12082158679017317No Hit
AAATTTTGGTGCTGCCCACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
GGATGAACAGTACCAGAAGTATAAGTTTATGGAACTCAACCTTGCTCAAAAGAAAAGAAGGCTAAAAGGTC30.12082158679017317No Hit
GGGTTAATGGCTAGTACTGTATTGGCTCTGTGAAAACATATTTGTGAAAAGAGTATGTAGTGGCTTCTTTT30.12082158679017317No Hit
CCTCTGGATGAGAAGAGCCTTGCTTTATTACTCAATTATCTTCACGATTTCCTAAAGTACCTGGCAAAGAA30.12082158679017317No Hit
GTTTAGGTTTTAAGCAAATAAAGTTCCAGTTAATGTGAAACTCTAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
AGGATGGAGATGAAGATGAGGAAGCTGAGTCAGCTACGGGCAAGCGGGCAGCTGAAGATGATGAGGATGAC30.12082158679017317No Hit
AGCCTGGCCAACATAGCGAAACCCTATCTCTACTAAAAATACAAAAATTAGCTGGGTGTGGTGGCACGTGA30.12082158679017317No Hit
GTCATAAAGATGAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
GTATTATATTAAAATACTAAAAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
TGGTCATGCCACTACTGTGTTCTAGTCTGGGCAACAGAGTGAGACCTTCTCTCAAAAAAAAAACCCAAAAT30.12082158679017317No Hit
GAATAAAGGTCCCATGCTCCACCCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12082158679017317No Hit
TTATTAGACATTCTGATGTTTGCATTGTGTAAATACTGTTGTATTGGAAAAGCATGCCAAGATGGAAAAAA30.12082158679017317No Hit
ACATAGCAGACTTTCCCAAATGTATTGATTACAAATAAACAGTTGTTACTTAGCAAGACCTGAAAATATGT30.12082158679017317No Hit
GGCGATGTGCGAGCTGATAGTGAGCGGCAGAATCAGGAGTACCAGCGGCTCATGGACATCAAGTCGCGGCT30.12082158679017317No Hit
GGGCATGGTGGCGGACATCTGTAATCCCAGCTACTCAGGAGGCTGAAGCAGAAGAACTGCTTGAACCTGGG30.12082158679017317No Hit
CGCATATGGCTTTCACACAGCCAGCTCCTGTTTTTGGGATACCACATCTGATGGAACCTGTACCGTAAGAT30.12082158679017317No Hit
GTCCTACGAAGCCCAGGATCCTGAGATCGCCAGCCTGTCAGGCAAGCTGAAGGCGCTGTTTCTGCCGCCCA30.12082158679017317No Hit

[OK]Adapter Content

Adapter graph