FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002
Sequences flagged as poor quality0
Sequence length71
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51217.055296469020654No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG170.5662891405729514No Hit
GGCTTCTGCTTTTGTTTTAAGGGATTAACTTCCCTGTCAAGTCCAAGAAGACTTGCGTATGAGAAGATTAC150.4996668887408394No Hit
GCTTCCAGTGGTGGCCGTGGACTTGGCTCGGAACTTAGTGGCACCAGAGTAACTCTAGTCAGTTACAGTAA130.4330446369087275No Hit
AGCAGGGTTGGGGGAGAGGCTGAGGAGAGTGTGACATGTGGGGAGAGGACCAGCTGGGTGCTTGGGCATTG130.4330446369087275No Hit
CACCCTGGGAGACAGTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCATAGCCGTGGGGTGACTTCCC130.4330446369087275No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC110.36642238507661556No Hit
GCATTTTTTCCAGAGTGGTGCAGTACCACTGAGGACTGTTGCTGTATTGATTAGGAAAAGAGACAGAGTAA110.36642238507661556No Hit
AAGTTTGTAAAATATCAGAGGATATATATATATTGTTTCTACGACATGGTATTGCATTTATATCTTTTTAC110.36642238507661556No Hit
GCCATGTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGAAGAAGAAGAAGCCTTT100.3331112591605596No Hit
GGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATACTTCACAACAATCCT100.3331112591605596No Hit
GGTTGGCTCCATGACCAAGATCTATGGGGGACGTCAGAGAAACGGCGTCATGCCCAGCCACTTCAGCCGAG100.3331112591605596No Hit
GGCCTTCGGAAAGAGAGAGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTGGCAG90.29980013324450366No Hit
GCAGTTGAGGTAGTGCCTAGTGTGTGAATTTGAGGTCATTTTCTAAACTGGCCGGGCACAGTGGCTCATGC90.29980013324450366No Hit
AATTTGTTATAATGAATGAAACATTTTGTCATATAAGATTCATATTTACTTCTTATACATTTGATAAAGTA90.29980013324450366No Hit
TTGCAAGAGATGGAGTAGAAGAAGTTTTGAAATTTGTATCCTAAATTAGCTTCAAGTAAGTGCCTAAAGAG90.29980013324450366No Hit
GACTGGGGAAACTTCCCCCTGCCTGGTACTGAAGTCCTGCTCTGCTCAACAGCTCTAGGAAGCCCATTTCA90.29980013324450366No Hit
AGTTCTTTTACCAAGATTTTTGTGTGACCATGTTGACTTCATTTGGATTGCCCTCTTTCAATTTCCTTGTG90.29980013324450366No Hit
GGAGCAGGGAAGTACAGAGTGGGATGGTCAGGACCTGGGCCATTGCAACCAAAATGGGGACTTCCTGGGTA90.29980013324450366No Hit
GTGCCCCACAGCAGAACCCGTGGGCGCTCGTGTTGTGCGGGCCCTGCTCCTCTGCCCCGAAACCACTGGCT90.29980013324450366No Hit
GTGTTTCAGATATTTCTCTAATTATGTACAACCTAAAATGTTGGTGTTTTGTATGGATCACAAGTGCAGCA90.29980013324450366No Hit
CCAAGATCTATGGGGGACGTCAGAGAAACGGCGTCATGCCCAGCCACTTCAGCCGAGGCTCCAAGAGTGTG90.29980013324450366No Hit
GATCCTAGTGAGGGATTGATGAATGTTCTAAAGAAAATTTATGAAGATGGAGACGATGATATGAAGCGAAC90.29980013324450366No Hit
GGGTGACCACCTGACCAACCTCCACAGGCTGGGTGGCCCGGAGGCTGGGCTGGGCGAGTATCTCTTCGAAA90.29980013324450366No Hit
GAGGATTCCTCTCACCCACTGAGAGTAGGTTCCTGAAATTCTCCTGCATCACATCTTGGTACAGCTGCCTC90.29980013324450366No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA80.2664890073284477No Hit
GCAGTATGCTGTATAGTAATGGCCTCAGCATCCTCTTCATACAGTAGCTGGGGAAAATGCCAGAATGTAGT80.2664890073284477No Hit
CATTACATAATATTTTGATTCTGTTACGTGTGTCTATAGATTGTTTCATTCTAATCAAGACCTAAGAAGGT80.2664890073284477No Hit
TTTAAAATCAGTACTTTTTAATGGAAACAACTTGACCAAAAATTTGTCACAGAATTTTGAGACCCATTAAA80.2664890073284477No Hit
GTGTAAAGTAATCTTGGAATATAAAGAATTTCTTCAGGTTGAATTACCTAGAAGTCTGTCACTGACTTGTG80.2664890073284477No Hit
TCTGATAGAATCCCCTGAAAGCAGTAGCCACCATGTTCAACCATCTGTCATGACTGTTTGGCAAATGGAAA80.2664890073284477No Hit
GTGCCTGCTTCTGGGGGCAGTGACCTTGTGAACCACTCATTTTTATGCAAGTGGCATCCCTAAAACCTGAG80.2664890073284477No Hit
GAGTTCGAGTGAGAACAGCCTGGTCAACATGGCGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCAG80.2664890073284477No Hit
GGAAGTTGAACAGTTTGATGTCGCAACTCACCTGCAAACAGCCCCGGAGCTAGTCGACAGAGTCTTTAATA80.2664890073284477No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGG80.2664890073284477No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA70.2331778814123917No Hit
AAATATTACTTCTCATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.2331778814123917No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.2331778814123917No Hit
TTGTATTACTTACTGCAAGAGATATTTCATTTTCAGCACAGTGCAAAAGTTCTTTAAAATGCATATGTCTT70.2331778814123917No Hit
CTCCATTCAGATCATGAAGGTGGAGGAGATCGCGGCCAGCAAGTGCCGCCGGCCGGCTGTCAAGCAGTTCC70.2331778814123917No Hit
GGCTGCTGCCCGCGACATTGTGCAGTTTGTACCCTACCGCCGGTTCCAGAATGCCCCTCGGGAGGCATTGG70.2331778814123917No Hit
GAAGAAAAAAGACAAAGATGACAAGGAGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.19986675549633579No Hit
ATATAACTTCTCAACCCCCTAGCCTATTTCATGCTTCTAGCCCCCTTGTATTCTTCTAACAAAATTTCCAG60.19986675549633579No Hit
GTCCCATATCTCAGTGTCTCCATCTGCTAGGGACAATCTTACTTCTTCAATAAAAGTGGAAACAATAAGAA60.19986675549633579No Hit
GGAACAGGCTTACTGATAAGAAGCTGAAACAAGAAATTCTCTTCATTTTATCCCAGTGCTATTTTATTGAT60.19986675549633579No Hit
GATTCAAAGTGGGCAAAGGCCAAGAAAGGAGAGGAAGCTTTATTTACAACCAGGGAGTCTGTGGTTGACTA60.19986675549633579No Hit
GCTTTGGGCAAGTGATTTCACCTCCATGTGCCTCAGTTTGCTAGTCTGTACAATGGGATGGTAATAGCACC60.19986675549633579No Hit
CACCATTGCAGATGCTTAAGTCAACTATTTTAATAAATTGATGACCAGTTGTTAAAAAAAAAAAAAAAAAA60.19986675549633579No Hit
CCTTCTAAATCTAAGATTCTGCTGAGGAGCCCCCGCTGGTCCCTCTGGGCATGCTGTGCTCGGACGGAAAG60.19986675549633579No Hit
GGCTGACCAGGCTGAGGCCCGCAGGTCTAAGACCAAGAAAGCACGCAAGCGCCGTGAAGAGCGCCTCCAGG60.19986675549633579No Hit
GTTTCTCAAACCAGGAGTTTATATTTATTTTTTAGAAAATACACACTTTTCAGGAGAAACCTGAGCATGAT60.19986675549633579No Hit
GTTTGGAGGGGAGGGAGAATTTATTAATAAAAGAATCTTTAACTTTAAAAAAAAAAAAAAAAAAAAAAAAA60.19986675549633579No Hit
GCCTTTTCAGAAGCGGCTGCGCACGGACTGCTGGAGATGGTGGAGTCCGTCATGCCGGTGGTGGTGTGCAC60.19986675549633579No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.19986675549633579No Hit
CTTTGTACAGTGTGATGCTCGAGCAATTGGCTCTGCTTCAGAGGGTGCCCAGAGCTCCTTGCAAGAAGTTT60.19986675549633579No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG60.19986675549633579No Hit
GTTACATTGTCTTACTCTCTTTTACTTCTCAGACACTTCCCCCACCCTCATAGAACCTGTTGCATGCAACT60.19986675549633579No Hit
CTTCCACGCTACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAA60.19986675549633579No Hit
ACATAAGCCATTCCCACCCTCCTAGGTTCACATCCAGGGCTGTGTCTTCCTTGGGGGAGGAGATGGTGTCG60.19986675549633579No Hit
AGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTCACAGGACTCAACATACTAGTCACAGCCCTAT60.19986675549633579No Hit
TGTGTTGCCTCTGTTACTGTTTGTATTGAATAAAAACATCTTCATGTGGGCTGGGGTAGAAACTGGTGTCT50.1665556295802798No Hit
CCATGTCAGACGCAGCCGTAGACACCAGCTCCGAAATCACCACCAAGGACTTAAAGGAGAAGAAGGAAGTT50.1665556295802798No Hit
GCATTTTGAATTACTTTTCAAAGAATACTGTTCATATGCATTGTTTTTGTGTTTCAAACTAAATACAGGCA50.1665556295802798No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT50.1665556295802798No Hit
CCTTTGATCCCAGCCATAAAGTACCTGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1665556295802798No Hit
CCTCCCTTCATTGTACTTTATCATTAAAAATCAACTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1665556295802798No Hit
CCAGAAAATTGCAGCAGCTCTAATGAGATTATTCTGTCGCGTGTTCAGGATCTTATTGAAGGACATCTTAC50.1665556295802798No Hit
ACGCGGGCATTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1665556295802798No Hit
GCCTTTTAAAAAACCCACAAAATGCTGATTCAGTTCAAAATTAATGCAAATGTTTCAAAACTGGGTTTCTG50.1665556295802798No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1665556295802798No Hit
GGATGTTATAATAAACATATTGTTACTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1665556295802798No Hit
CCACCATCCGCAGAGATGCCCCTGCTGGCCGCAAAGTGGGTCTCATTGCTGCCCGCCGGACTGGACGTCTC50.1665556295802798No Hit
GGCCATTGGCCTCCTCGTGGCCATCTTCCACAAGTACTCCGGCAGGGAGGGTGACAAGCACACCCTGAGCA50.1665556295802798No Hit
CGCGCCTCCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTAAAAAAAAAAAAAAAAAAAAAAA50.1665556295802798No Hit
GGGTTATACTGGTGTCCGATGTGAACACTTCTTTTTAACCGTCCACCAACCTTTAAGCAAAGAGTATGTGG50.1665556295802798No Hit
CCTTCACTGAACATGCCTAGTCCAACATTTTTTCCCAGTGAGTCACATCCTGGGATCCAGTGTATAAATCC50.1665556295802798No Hit
GGTTTCAATGTGGGCTGCAGCTTTTTGTGCTCCTTCTCTGGTTTGCAGTGTAATGAGTTCATAAGCTAAAT50.1665556295802798No Hit
CCGCTATGCAGGGTAGCACTGGGAACAGGAGACCCACCTGAGGCTCAGCCCTAGCCCTCAGCCCACCTGGG40.13324450366422386No Hit
GTATCCTCAGTTGACTCATTCACTCAGTTCACACAAAAGATGTTGGACAATTTCTACAATTTTGCTTCATC40.13324450366422386No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAG40.13324450366422386No Hit
GCCTGGCCAACATGGTGAAAACCCATCTCTACTAAAAATACAAAAATTAGCTGGGCGTGGTGTCATGCGCC40.13324450366422386No Hit
GATCTCATTGATGTGATGTTACATTCCCTTTAATCTGCCAACTGTGGTCAAAGTTCATAGGTGTCGTACAT40.13324450366422386No Hit
CACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAAT40.13324450366422386No Hit
AGACCATCCTGGCTAACATGGTGAAACCCCGTCTTTATTAAAAATACAAAAAAAATTAGCTGGGCATGTTG40.13324450366422386No Hit
ATGTTAAGAAATATATTTATTTGCTAATATATTTATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.13324450366422386No Hit
GGGCCTGGGCCCCGTGGAGCCTCAGTGCCCGTTTGGCCTGCTGCTCTCGCTGACAATAAAGAGCCCTTGCG40.13324450366422386No Hit
GCGTTTAAAGGCTGCTATTACTATACGATCTCTAATAAATCCAGATAGATATATCATTGTGGTGGAACATG40.13324450366422386No Hit
GTATATCTTTTAACTAGGAACTTTTTACAAATTAGCATTTTTCTTCCAAGAGTCATGCATATAATTCTTAG40.13324450366422386No Hit
CAATAAAGGATGGTCTTGGGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.13324450366422386No Hit
GGTCCTACAGGCAGGTGCTGGGAAAGGCCTGGCCAGCAGGTAGCCTGTGTGTTTGACAAACAGCAGCTGGC40.13324450366422386No Hit
GATTTCTGAATCATGTTCTAGTTGCTTGACCCTGCCACATGGGTCCAGTGTTCATCTGAGCATAACTGTAC40.13324450366422386No Hit
GCACAAACTTGCTGCTGCTTAGATCACTGCAGCTTCTAGGACCCGGTTTCTTTTACTGATTTAAAAACAAA40.13324450366422386No Hit
GGACAGGCTGCATTGGGGCCAAACACAGAATCAGCAAAGAGGAGGCCATGCGCTGGTTCCAGCAGAAGTAT40.13324450366422386No Hit
GTACAGAAATCATTAAATTATTAAGTTGTACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.13324450366422386No Hit
GAGTGTGACAGACGACTCAATAACCTCATTTGTTTCTAAACATTTTTCTTTGATAAAGTGCCTAAATCTGT40.13324450366422386No Hit
TTCCTGCCTTCTTATATTAATAAGAAAAATAAAACAAAATAGTGTTGAAAAAAAAAAAAAAAAAAAAAAAA40.13324450366422386No Hit
GTGTAGAGTTCTTATGAGTTTTTTCTTTGGTTGTTTGTTTATTTGTTTATGCAATCATTGTTAAGTTGTTG40.13324450366422386No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.13324450366422386No Hit
GCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTTTGGATAAAAAAAAAA40.13324450366422386No Hit
TTTCTGGAAGTGTGAAAATGTTAATGTTCAACAAGCAACATTAAAAATAGATTTGAAACGAAAAAAAAAAA40.13324450366422386No Hit
AGCCTAGACCACGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCATCTCAAAAAAAAAAAAAAAA40.13324450366422386No Hit
GAATGAGAGTTTGGATGGATGAAAAGTAAACCTAGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCAC40.13324450366422386No Hit
GGAGCATTTTGGATTTTCAGATATGGCATGCTGAACCAGTAAGTATATAATGCAAATATTCCAAAATCCTA40.13324450366422386No Hit
GCAAAGAATCCAGGAAAGAATTGCCAGAGAAGAAATTAAAAAGAAGATATTATATGAAGGTACCCACCTCG40.13324450366422386No Hit
GCAAAGAATCCAGGAAAGAATTGCCAGAGAAGAAATTAAAAAGAAGATATTATATGAAGGTACCCACCTCA40.13324450366422386No Hit
GGAGATGAAGATGAGGAAGCTGAGTCAGCTACGGGCAAGCGGGCAGCTGAAGATGATGAGGATGACGATGT40.13324450366422386No Hit
GGGAAATCATTAGAAAGGAGGCTGTAGAATATTTGCCGAGCCTCTACTGTATACCAGGGGCTAACTCACCA40.13324450366422386No Hit
ATCCAAGTGTTATACCAACTAAAACCCCCAATAAACCTTGAACAGTGAAAAAAAAAAAAAAAAAAAAAAAA40.13324450366422386No Hit
CATGCTGCAACCATGGTATACAACAAAAATACATTTCTTTGGTGATTGAAATTAAGGCCGTATTTACAATG40.13324450366422386No Hit
GTTACAGGGATCATGTAAACTTGCTGTTTTTGTTTTTTCCTGCCGGGTGTTGTATGTGTGGTGACTTGCGG40.13324450366422386No Hit
CTCTTGTAGGTTCCATCAATGGTGAGCACCAGCCTGAATGCAGAAGCGCTCCAGTATCTCCAAGGGTACCT40.13324450366422386No Hit
ATATGAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.13324450366422386No Hit
GGCAAGAGGGTTGCTTAGGCCCAGGAATTCAAGGCTGCAGTGAGCTGTGATCACACCACTGCACTCCAGCC40.13324450366422386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)