FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13669
Sequences flagged as poor quality0
Sequence length71
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4883.570122174262931No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA1761.2875850464554832No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1250.9144780159484966No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT1080.7901090057795012No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC860.6291608749725657No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC730.5340551613139219No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT640.4682127441656303No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC540.3950545028897506No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA500.3657912063793986No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC460.33652790986904674No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC460.33652790986904674No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT350.25605384446557905No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.2414221962104031No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320.23410637208281515No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA310.22679054795522718No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT290.21215889970005122No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.1975272514448753No Hit
AACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTAC260.1902114273172873No Hit
TAGTGAGACCTTGTCTTTACAAGAAATTTAAGAATTAGGGCATGGTGGCATGTGCCTGTAGTCCCAGTTAC250.1828956031896993No Hit
CTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC250.1828956031896993No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.1828956031896993No Hit
ATGTTATACACATGACTTACATGTGCAATTTTTAGAAATATCAAATTAGGTATAAGATTTTGAAGAAAGAA230.16826395493452337No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGC220.1609481308069354No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA210.1536323066793474No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC210.1536323066793474No Hit
CACGCATTGGGCGCGCCGGGACTGTGAGACGACAGGCTGTGGATGTGTCCCCCCTGCGCCGTGTGAACCAG210.1536323066793474No Hit
CAATAACAACATGTGCAAACCAAGCACATTACCCTGATACGGTCCTTGACAGAAAGCTCTTTCGGTTAGCT200.14631648255175944No Hit
ATGTTGAGTGGCCCAGGTCAGTTTGCTGAGAACGAAACCAATGAGGTCAATTTTAGAGAGATACCTTCACA200.14631648255175944No Hit
TCTCCTTTGGCATGTTTAATTGTGATATTTGACAGACATCCTTGCAGTTTAAGATGACACTTTTAAAATAA190.13900065842417147No Hit
GATAAAACCCGTCTCTACTAATACAAAAAAAATTAGCTGGGTATGTTGGTGCAGGCTTGCAATCCCAGCTA190.13900065842417147No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCA190.13900065842417147No Hit
GTGCATTATAGGAAATTGAAAAACACAAGCAAAGAACAAAGTCATTCACAATCACAAGCAGTTTATAGTTT190.13900065842417147No Hit
CTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG180.1316848342965835No Hit
GGCTGTGGAGGAGTGGAATATTGAGAAGGCTGAGGAACTCAGCAACCAGCTAGCTACTCGAGAGCTTGGTG180.1316848342965835No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCC180.1316848342965835No Hit
TCATCAAGGAGTAAGACAATGTCAGATTTTCAGTTTTTAAAAGATGATTTCTAATAAAGAATTATAACTGT180.1316848342965835No Hit
ATTATTGTGTAAGGAAGACTGATGTAGATGTTCTGTGCTGTCCTGGACCATGTTAATTACACTTACGACGT170.12436901016899553No Hit
CTGCATGTCTCTGGACTTAGTATAAGCAATTTTTCCTTCAAAAATAAAATCTTATCAGTCACCACTAAATC170.12436901016899553No Hit
GTACAGTCCACCTGAATTAGGGCCATGGTTTTTACTTTTCCCTTCATGGTCTCTTTTAGCAAGTAACAGGC170.12436901016899553No Hit
CTATGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGATCGA170.12436901016899553No Hit
CTTTATAGCAGTGTGAAAACAGACTAATACAGAAGTATATGTTAACTTTCTCTGCTGAGCATGTTGGTGCT170.12436901016899553No Hit
TTCCAGGGCACTGAAAAACACTAGAAGGAAGTGCCCTGATAAACTACAGCATTTAATACTTTAGACTCACT170.12436901016899553No Hit
GTACAAATCTGTAAAGGAGGGCTTTATCGTCAGCACAGAGCAGGAAAAACCTAAATAGAATGAAGGCAGCT170.12436901016899553No Hit
ATCCATTTCACTGTGTGTGGATGGATAGCAGAGGGTACCACGCAGTCCTTGAGGCAGTCCTGTGTGATTCC170.12436901016899553No Hit
GGCTAACACGGTGAAACCCCGTCTCTACTACAAATACAAAAAAATTAGCCGGGCGTGGTGGCAGGCACCTG160.11705318604140758No Hit
GTATAATATCTTGCAGAAATAGTAGCAAATTTAAAGAAGCATGTATAAGCATTTCTTTAACTGACAACCTA160.11705318604140758No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGATATGTCTCCATACC160.11705318604140758No Hit
TCTTAATACACTAGAAAGGGGAAATAAATTAAAGTACACAAGTCCAAGTCTAAAACTTTAGTACTTTTCCA160.11705318604140758No Hit
GCCTTTTTGTATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCCTTTTTAATATGCTTAAAATAAGC160.11705318604140758No Hit
ACAACAGAGAGACTGTGGCAGAGGCAGGACTGCAGATCTATGGAAATTGCCTGGAAGAGTCAGCTGTAAGG160.11705318604140758No Hit
CTTCTAGATCCTGTGTGTAGGCACAACATCCAATTTTATGGGACTGAGACTGTACAGTATGTATCATGATT150.10973736191381961No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10973736191381961No Hit
CTTTACATTCAGCAAGTTTAAATCCTTACATGATTTTTGAAACCTTATTAACTGAAAATATTGCTTGCTGA150.10973736191381961No Hit
GAGCATAAGTCTACTGGGTGCCTTTACATGCCAGAGGCTGATGCTGCACTGTTGATGTCATGTGAGGAAAT150.10973736191381961No Hit
GAGAAAGGAGGAAAAAAGCTAGAATTCTATGCACTACTACACAGGGGCCTAGCACCCTCCAGCTTCCAGCA150.10973736191381961No Hit
CTCCTGCTGTTCTTTCTCTGTGCCCCCTAATTGCTTGGTAATCTAAAATTATTCTGACTTTCCACATACTG150.10973736191381961No Hit
GTATGTTAAGTCTGTTTTTGAAAAGTAAAATGAATACGAGTTCACAATCACGAAATACAGATTGTTAAAAG150.10973736191381961No Hit
GAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTA150.10973736191381961No Hit
TCTGTTCAGCGGGAGACACACATTTGGAACTAACCTTCTGAGCTTCCTCAAAAAGGCTATCAACAAAATAT150.10973736191381961No Hit
GGTATACATTCTCTTTGTCATCTGGACAGAGCTTCATGTGGTGAGGGCAATTTTAAGGCACTGTATAGACC150.10973736191381961No Hit
CTTATAACCCCAGCTGTGGTCTCTGAGAGACTGAAGATTCGAGGCTCCCTGGCCAGGGCAGCCCTTCAGGA150.10973736191381961No Hit
GGTCTCTCTGGATAGTTGTAGCACCTTTCCTTATAGAATTCTATTACCAGGCCGAGCCTGGTGGCTCACAC150.10973736191381961No Hit
GCATTAAGAATCTGTCTCTTTGCCTGGTGTGGTGGTTTACACCTGTAATCCCAGCACTTTGGGAGGCCAGG150.10973736191381961No Hit
ACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTA150.10973736191381961No Hit
GAGGGAATAAGGAATAAATTTCAAAAAATGTACAATTTCCTTTAGAGAAGTTTCAGAACCAAGACTAATTT140.10242153778623163No Hit
GTCTTAAAAGCATAAAATATTTGGGCCAGGTAATTACGCCTGTAATTCTAGCACTTTGGGAGGCCGAGGTG140.10242153778623163No Hit
CTCCTTTCCTTCCCAGGCCCTTTGGCTTCTGATTTTTCTCTTTTTTAAAAACAAACAAACAAACTGTTGCA140.10242153778623163No Hit
GGTTTTTCTTTTTTCCCACAGAGCTCGGTGGTGTTGATTCCATACAGTTTTTGTTCAGACAGGAAGGGATA140.10242153778623163No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAAT140.10242153778623163No Hit
ATAGGGGACAGAAATCAGGTATTGGCAGTTTTTCCATTTTCATTTGTGTGTGAATTTTTAATATAAATGCG140.10242153778623163No Hit
AACTTTCCCTGTTCAGAGCATCAGATGAAGACTTCATTGGGTTTTATAGTGGCTTTCTGATTTTTGGTAGT140.10242153778623163No Hit
TGGCTACACAATAATGCATTGCCTCTATCATGTTAGAACGTGCATTAGACTCAAATACAAAAACCATGAAA140.10242153778623163No Hit
GAAGAATTATCTGCTTTGAGAAATAAAGAAATTAGAAGGTGTAAAAAAAAATTTTTCAAACCCCAAATAAT140.10242153778623163No Hit
TTGGATAACAGTCTTGCATGTTTATCATGTTACAATTTAATATTCCATCCTGCCCAACCCTTCCTCTCCCA140.10242153778623163No Hit
CTAAAAATACAAAACTTAGCTGGGTGTGGTGGTGGATGCCTGTAGTCCCAGCTCCTCAGGAGGCTGAGGCA140.10242153778623163No Hit
ATATAAATCATGAGGCTTCTAAACATCCTTTTTAAAGACAGCGTGTCACTCATGAAATTGGATTAAGTAAA140.10242153778623163No Hit
CACGCACTCTGATTTCACCTACTTAACTAAAAGTTGCAGCAAAATCCCTATTACAGGCCAGGCGTGGTGGC140.10242153778623163No Hit
AGGATACAGAAATCTGCTGTGTTTTGACTAGTTATCCCTGTTTTATTTTTTTAGATGTGCTTAATTTTATG140.10242153778623163No Hit
GCAGTCAGGTGGGCGGTGTGTCTTTCCAGAAGGTCACGTGGAAATGTCTCGGGACTTGGGTCCCGGAGTGC140.10242153778623163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)