FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319
Sequences flagged as poor quality0
Sequence length71
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27020.47005307050796No Hit
GTCTTAGTTACTGATTTCATTTTCAATAAATGTAGGTTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.6823351023502654No Hit
GCTGCCTCAACTGGTAGACGCTCCCAAGAAGTCAGAGGCAGCTGTCGGGGCGGAGGTGTCCATGACCTCCC80.6065200909780136No Hit
CCCTGGGAGTGGGTGGAGGCAGCCAGGGCTTACCTGTACACTGACTTGAGACCAGTTGAATAAAAGTGCAC70.530705079605762No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT70.530705079605762No Hit
CCGCACTCCAGCCTGGGGGACAGAGTGAGACTCCGTCTCAAAAAAAAAAAAATTATTCAGTGAAAGTGGAT60.45489006823351025No Hit
GAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGATGACATGGGCTTTGGTCTTTTTGA60.45489006823351025No Hit
GTTCTGATTTTTTGGTTTGTTTTTTAAGGAACTGTAATGAACAAATGTCAGGATATCCAATGCCAAATAAA60.45489006823351025No Hit
GCTTTGTGGTGACCTGATGTAAACAGTGTCTTCTTAAAATCAAATGTAAATCAATTACAGATTAAAAAAAA60.45489006823351025No Hit
AGATCGAGACCATCCTGGCTAAAACGGTGAAACCCCGTCTCTACTAAAAATACAAATAATTAGCTGGGCGT50.37907505686125853No Hit
GTATAACAATACTAGACTATAGACAAGTAAAAATTTATAAATATTAAGAATGTATATACATGTCACCATGG50.37907505686125853No Hit
GAGGGCCTTCTATGCTGAGCTTTATCATATCATCAGCAGCAACCTGGAGAAAATTGTCAACCCAAAGGGTG50.37907505686125853No Hit
GGATTCAGTATTCCTGGCCCAGAGGGCCCTTGCTGGCTACTGGGTGTTAGTTTGCAGTCCTGTGTGCTTCC50.37907505686125853No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG40.3032600454890068No Hit
GCTATTTTATATTATGACTGCTTTTTAAGAAATTTTTGTTTATGGATCTGATAAAATCTAGATCTCTAATA40.3032600454890068No Hit
GACCAGCCTGGCCAACATGGTGAAACCCCGTCTCCCTACTAAAAATACAAAAATTAGCTGGGTGTGGTGGC40.3032600454890068No Hit
GATGTACATATTTACGAAGCACAGAAGAGAAACCAGAAGGAGGACTTATAATTGGCTATAATTTATAAGAA40.3032600454890068No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA40.3032600454890068No Hit
GGTTTGAAGTTAACTGAAATATGGAGATTGAATTTATACTCAATTCTTGTGAAAAGTCTTGCTTAATAAGT40.3032600454890068No Hit
GTAACTTATATTGAAGACAATTTGATACATAATAAAAAATTATGACAAAGAAAAAAAAAAAAAAAAAAAAA40.3032600454890068No Hit
GTACAGCAGTTACAAGAGGATGTGGACATGGAAGATGCTCCTTAAAAATCTCTGTAACCATTTCTTTTATG40.3032600454890068No Hit
CTCATATCCTGTTCAAGTTAATCAACATCAAGCACATGGGGATGCTTTAGGGTGAGTCTATAGTACAAAAT40.3032600454890068No Hit
GACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3032600454890068No Hit
GGCGCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAACCCAGGAGGCGGAGGTTGC40.3032600454890068No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3032600454890068No Hit
GGTCCCAGCACCGATGGCATTCCCTTTGCCCTGAGTCTGCAGCGGGTCCCTTTTGTGCTTCCTTCCCCTCA40.3032600454890068No Hit
AGGCTTACCCCTCCTGCCTCCCTTCCCCTGCCTGCTGCTGGGGGAGATGCTGTCCATGTTTCTAGGGGTAT40.3032600454890068No Hit
CTACTCAGCATGGAAACAAGATGAAATTCCATTTGTAGGTAGTGAGACAAAATTGATGATCCATTAAGTAA40.3032600454890068No Hit
AGGTTGAAACTATGTAAATGGTTTTTATGGAAATTATCAGTTACAATATTTTAAAGGTGTAGAATGGCATC40.3032600454890068No Hit
ATGCATGGGCCGCTGCTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22744503411675512No Hit
GGTAAAGGCAGACCGAGATGAATCCTCACCATATGCTGCTATGTTGGCTGCCCAGGATGTGGCCCAGAGGT30.22744503411675512No Hit
CCTCTCTTCTCTCCCCCTCAGCCTTTCTCTCGCCTGCCTTCTGAGCTGCACTTTCCGTGGGTGCCTTATGT30.22744503411675512No Hit
AAATAAAGTTAATTCAAATTTTGTGTTAAGTGTTGTTTTTTACAAATCTTTTGGTTTTGAAAGGAATTGGT30.22744503411675512No Hit
CCTCAGTCTGGAGCACATCGGCCGGAGGCGGCTCCGCTCGGCCGGCGCGGCGCAGAAGAAACCCGCGGCGC30.22744503411675512No Hit
CCCACATCAGCAAGGGTACGCTGGGCAAGTTCACTGTGCCCATGCTGAAAGAGGCCTGCCGGGCTTACGGG30.22744503411675512No Hit
GATAAATTTCAATTAGATTTAAAATAAACATTATGTCCACCTTTTAAGTTAATGAAATAAAATTTGAAACT30.22744503411675512No Hit
CCCTTCGCCTGTGTGCCTGGAGCCAGTCCCACCACGCTCGCGTTTCCTCCTGTAGTGCTCACAGGTCCCAG30.22744503411675512No Hit
GCTACCCAGCAGTTCAAGCCGGATGCGCAGTGATGGTTTTGATGAAGAAAGTCAAAGATACTATTGGAGGC30.22744503411675512No Hit
AGGTTGAGCTGAGAAGAGGCCAGTGCACTCAAGCCTGAGCAATAAGAATAAAACCGAGTAGAACAAAATAA30.22744503411675512No Hit
GGCCCCACTTCCAGCCCCTCCATTCTGAGTACACTTCAGAGGGCTCTGGCAGGGTGAGGGGGTCTTGGTGG30.22744503411675512No Hit
GGGTTCGAGGGCACGAGTTCGAGGCCAACCTGGTCCACATGGGTTGAAAAAAAAAAAAAAAAAAAAAAAAA30.22744503411675512No Hit
TCCCTACACGACGCGGGTGGGGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22744503411675512No Hit
GATGATAGTGACGAATGTCTGGCAGTGAGGACACATTTTGGCATTCTTGCTGACTCTGACAGAGTGATTCT30.22744503411675512No Hit
GTGCCAGGATTACAGGCGTGAGCCACTGCGCCTGGCTGAGCATTTGCTGTTAATACGTTTCTGATGCTGTG30.22744503411675512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.22744503411675512No Hit
CTCCTCCCGGGACCCTCCGGGTTCTCTGTCCGCCAAGAAGGTCCGGACTGAGGAGAAGAAGGCACCGCGGA30.22744503411675512No Hit
TGGTTGCAGTGAGCTGAGATGGTACCACTCCACTCCAGCCTGGGTGACAGAGGAAGACTCCATCTAAAAAA30.22744503411675512No Hit
GCTACATTTGAAGCCTGTACAAAAGCTTATCCCTGTAACACATGTGCCATAATATACAAACTTCTACTTTC30.22744503411675512No Hit
CTGATAACTTGTTCCTTTTCCTGACTCCTCCTTGCAAACAAAATGATAGTTGACACTTTATCCTGATTTTT30.22744503411675512No Hit
CAAAAAGCTAAGGGCAAAATGTACAAACTTAGAAGAAAATTGGAAGATAGAAACAAGATAGAAAATGAAAA30.22744503411675512No Hit
GCACTTGTTCCTTGTTTCCTCTGGGTTTTATAGTGATACAGTGGTGTCCCCGGGGCCAGGCGCGCTCTGCC30.22744503411675512No Hit
CTGTCAGATTTATCTTTGTATCTTCCCCAGCACCTAGTGTAGTGCCTTGCACATAATAGGCGCCCAATAAA30.22744503411675512No Hit
AATAATGACTGAGATGGAGATATCTCGAGCAGCAGCAGAACGCAGTTTGCGGGAACACATGGGCAACGTGG30.22744503411675512No Hit
GCCTCACAGATGATGTCCATGTTTTGTGTGAATGAAACTCAAACACTCAAGAAAAAAAAAAAAAAAAAAAA30.22744503411675512No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22744503411675512No Hit
GTGTAAGTGCTTAAAATGGAATAAATTGCTTTTCTACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22744503411675512No Hit
GACCACCACCCGCCGGATAGTGGATGACAAAGTGGTGTCTGAGACCAATGACACCAAAGTTCTGAGGCATT30.22744503411675512No Hit
GTAGATAAATGTCGTCACTTACAGGAAAATTTGAATTCTCATGTTCTGATTCTTTCTCTGCAACTTTCTAA30.22744503411675512No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC30.22744503411675512No Hit
GTACAAGACCACACCGGATGTCATCTTTGTATTTGGATTCAGAACTCATTTTGGTGGTGGCAAGACAACTG30.22744503411675512No Hit
GAAAAAAAATACAAAAATTAGCAGGGTGCCTGTAATCCCAGTTACTCAGGAGGCTGAGGCAGAAAAATTGC30.22744503411675512No Hit
GCCCATTACAAGCTGGTGCCCCAGCAGCTGGCACACTGAGCACCTGCACTAAATTACTCAAAATGTGCTGT30.22744503411675512No Hit
ATGTACTGCTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GGAGCCACTCAAAGTCTCCCGAAAGGTCTAAGAAGAGCCACAAGAAGAGCCGGAGAGGGAATGAGTAATGG20.1516300227445034No Hit
ATTGTACTCAATGGTATCATTACAATTTTCTGTAAGAGAAAATATTACTTATTTATCCTAGTATTCCTAAC20.1516300227445034No Hit
TCCCTACACGACGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GGCTGGCTGGGAGATGTTTCGGGCTGGACACAGGACCCCACTCAGTGATTCTGGGGTTACACAGAGGTATC20.1516300227445034No Hit
AATCATCCCTAATGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
ACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GGCCTGTCTGCAAACCAGTGTGCCGTGCCAGCCAAGGACAGGGTGGACTGCGGCTACCCCCATGTCACCCC20.1516300227445034No Hit
CCATACCTGTGCATGAGCTGTATTCTTCACAGCAACAGAGCTCAGTTAAATGCAACTGCAAGTAGGTTACT20.1516300227445034No Hit
GGCAGCAGCATACAATAAAACTTAGTATGAAACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GGTTGTAGTGAGCCGAAATCACACCATTGCACTCCAGCTTGGGCAACAATAGCGAACCTCCATCTCAAATT20.1516300227445034No Hit
GTCAAGGCCTGAGGGGCGCATTGTCAATAAAGCACAGCTGGCTGAGACTGAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GCGGGCGGCGGAGGCGGTGGGAGCGCCGGAGTTGGATATGCGCACATCGGGTGGTGTCCCCCGCCTGCCGA20.1516300227445034No Hit
TATGACAATTAATGATTAAAGACTGTGATTGCCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GCTACCAAGAAACCAGCCCCTGAAAAGAAGCCTGCAGAGAAGAAACCTACTACAGAGGAGAAGAAGCCTGC20.1516300227445034No Hit
GATGTGTCATGGTCTGATACTCGTAGGACCCTCCGAAAAGATCACCGCTGGGAATCTGGATCCTTATTGGA20.1516300227445034No Hit
TGTGCAAGGGGAGCACATATTGGATGTATATGTTACCATATGTTAGGAAATAAAATTATTTTGCTGAAAAA20.1516300227445034No Hit
ATACAAGACAGGCAAGAACAAGTGGTTCTTCCAGAAACTGCGGTTTTAGATGCTTTGTTTTGATCATTAAA20.1516300227445034No Hit
GAATTAAATTGCAATAATGAGATAAATGGTCAGACTAATGAAACAAATACTGAAATGCAAAGAAATAAACA20.1516300227445034No Hit
GGCTCTCCAGCTGGAGTCCATGGAGAGGCCAGAGGCTGGCGGCCCTGCCTGGGCCTTCGGAGCCTCCTGCC20.1516300227445034No Hit
GGATCAAGCGTGCTTTCCTTATCGAGGAGCAGAAAATCGTTGTGAAAGTGTTGAAGGCACAAGCACAGTGT20.1516300227445034No Hit
CTTTTAAAATAAAATCCTTAACTAAATTAAATTTAACAGTGTTTAATTGAACAAAGAACAGTTCACTAATT20.1516300227445034No Hit
GCTAAAAACAAGACTGGGGCTGCTCCCATCATTGATGTGGTGCGATCGGGCTACTACAAAGTTCTGGGAAA20.1516300227445034No Hit
GAACAGTTTGCCCACACTTAGTTTGTTGGTCTTATGTAAAACATTGGCTCAAAATAAAGTACACACTGATT20.1516300227445034No Hit
GTCTGATGATGACATGGGCTTTGGTCTTTTTGACTAAACCTCTTTTATAACATGTTCAATAAAAAGCTGAA20.1516300227445034No Hit
ACACGACGCGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
CGACGCGGGAACACAGATAGAAACCACACCAACAGAGACTGCTTCCAGGAAAACCTCTGACGTGGTACTGA20.1516300227445034No Hit
GCACTTGTTCCTTGTTTCCTCTGGGTTTTATAGTGATACAGTGGTGTCCCCGGGGCCCGGCGCGCTCTGCC20.1516300227445034No Hit
CTAGGGAGTATGCTGGAGGCAGAGGGCAAGGGAGGGGTGGTATTAAACAAGTCAATTCTGTGTGGTATATT20.1516300227445034No Hit
ATTTTGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
ACTCCGCGCCGATGCCACCGGCCTGTGGGTCTCTGAAGGGACCCCCCCCAATCGGACTGCCAAATTCTCCG20.1516300227445034No Hit
GGACTCCCTCATTCCTAGAAATTTAACCTTAATGAAATCCCTAATAAAACTCAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GCATTCCATCCGCTCTGCAGCCCCAAGCATCTTTCCAGTGGGGCCCCCATTCACGTTGGTCCTCAGGGAAA20.1516300227445034No Hit
GCTAGGGTTTTCAATTCCAATTCTTGTATTAAGTTTTTTCCTTTCAGTTTTAGGTGCGAAAGTAATCAGTC20.1516300227445034No Hit
CTATTGGCCTGCATGTTCCCATCTGGCCAAAGAAACCAGCAATTCTCAACCACTACTGCATATGAGAAGTC20.1516300227445034No Hit
ACCCCATGGCCTCCATGACTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GATTAGTTGTACAACTAGTTAGAAGTTTCAGAATAAACATGCATTTCATAACTGTCAAAAAAAAAAAAAAA20.1516300227445034No Hit
CAAGGTATTTCTTCTTATTGTAGAGACTGCATCAACCCGACATTCCTTTCTTATACCAATGTGAAATTTCC20.1516300227445034No Hit
GAACTTTGGGACTGGGAGTTTTTGGCTGAAATCCTCTGTCATGGGACGAGGGTACAGTAAAGAAGCTCTAT20.1516300227445034No Hit
TTTTAATCCAAATAAATACAGAGTTTGCAAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GGCAAGAAGCCTGTGGTAGGTAAGAAAGGAAAGAAGGCTGCTGTTGGTGTTAAGAAGCAGAAGAAGCCTCT20.1516300227445034No Hit
GGGTAATAAACTTTAATGAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit
GTTTAGCGGAACTTGTATTCAGCCTGACACGCTTTGCCAGGAACAAACCTCATGTGAAAGAAAACAAAATA20.1516300227445034No Hit
GCTTTAAACTGCATTTTTCTAATGGGCTAAACCCAGATGGTTTCCTAGGAAATCACAGGCTTCTGAGCACA20.1516300227445034No Hit
GGGGCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1516300227445034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)