FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003
Sequences flagged as poor quality0
Sequence length71
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15014.955134596211368No Hit
GCATTAGAGGGCGGAAGCGCTATCCGAGCAGGATGCGGTTCGTGGTTGCCTTGGTCCTCCTGAACGTCGCA121.1964107676969093No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAA111.0967098703888334No Hit
TTTAATGACTTTCCCTGCTGTTACCTAATTACAAATTGGATGGAACTGTGTTTTTTTCTGCTTTGTTTTTT100.9970089730807578No Hit
CTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGT80.7976071784646062No Hit
GCCATAAGCGGTATAAAATCCAAAAAAAGGTTCGAGAACATCATCGAAAATTAAGAAAGGAGGCTAAAAAG80.7976071784646062No Hit
ATATAATTCTTTTGACTTTGTTTCTCTTGTGTAGTGGTAAAATGGTAGCTGGTGCTTATTGAGATTTGCTG80.7976071784646062No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG70.6979062811565304No Hit
GCCCCTGTGGCTGCTGCCACCACAGCTGCTCCTGCTGCTGCTGCAGCCCCAGCTAAGGTTGAAGCCAAGGA70.6979062811565304No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.6979062811565304No Hit
ATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGT70.6979062811565304No Hit
CATATTAGCATCATCAACCCTCTAATTCACCTTATGGGGGAAATGCTTCTTTTTGTTTGTGATAGCTATTT60.5982053838484547No Hit
GCACTGGGAGACCAGCAGGACTCCTGAGAACTGTGCTGGTGGAGAGGTCCTAGAGCCGGCGAGCGTTTGAG60.5982053838484547No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC60.5982053838484547No Hit
CCCATCTCTACTAAAAATACAAAAATTAACTGGGCATGGTGGTGGGCCCCTGTAATCCCAGCTACTTGGGA60.5982053838484547No Hit
GAACAGAAAACTGCTGGCTGTTGATTTTGTCTGGTTCCTTTGCCAACATCTGGGCACACCCTTTGCCCAGA60.5982053838484547No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC60.5982053838484547No Hit
GACAGGGAGGGTATTCTCAGCAAAGGAACTAGTGTAAGTACAGACACTAAGGCAAGAAAAAATATAGTTCT60.5982053838484547No Hit
GAGTGTGGCCCGCCGGGTCCTCCAAGCCCTGGAGGGGCTGAAAATGGTGGAAAAGGACCAAGATGGCGGCC50.4985044865403789No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC50.4985044865403789No Hit
CGGAAGGGTCGCGGCTTTGGCTCCGAAGAGGGGTCCCGAGCGCGGATGCGTGAGGATTATGACAGCGTGGA50.4985044865403789No Hit
ATATTAAATATTTGGTTTCAAAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.4985044865403789No Hit
ACACGACGCGGGTATGCATGAGCTGTGTAAATTTTGTGAATATGTATTATATTAAAACCAGGCAACTTGGA40.3988035892323031No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA40.3988035892323031No Hit
AAGATGAGGTTACAAATTTTGTTAAGTTCAGCCATTATTACTTTTGGTATCCCAGAACATGACAAATTATG40.3988035892323031No Hit
CTGAGAAAATAGGATATAGTTTTGATTTTTTTAATTGTAAAATATTTTGGAGGGAGAACAAAATCTTTTAA40.3988035892323031No Hit
GGATATAGTAGTTACTTGTGACAAGAATAATTTTGGAATAATTTCTATTAATATCAACTCTGAAGCTAATT40.3988035892323031No Hit
GGGAAGGGAAATAGGGCACCATGGTCCGGTGGTTTGTAGTTCCTTCAAAGTCAGGCACTGGGAGCTAGAGG40.3988035892323031No Hit
GGGCTGGGGTCCTCCTGTGCTATTTGTACAAATAAACCTGAGGCAGGAAAAAAAAAAAAAAAAAAAAAAAA40.3988035892323031No Hit
AGATACTCCTCGAGAAGAGCAACTCCAAGACACATAATTGTCAGATTCACCAAAAAAAAAAAAAAAAAAAA40.3988035892323031No Hit
GTATATGTATTTTTTTAAGTGACCTCCTCTCCTTCCACAGACCCCACATGCCCAAAGGCCTCGGGACTTCC40.3988035892323031No Hit
TCCCTACACGACGCGGGAACTCACAAAAGACAGATTAACTGGAGAAAAGGCATACAATTTTTACTGATGTA30.29910269192422734No Hit
GACCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACAGAATCGCCGGACAGGTGGC30.29910269192422734No Hit
ATATGTTATAACTTATCCTTTAAAAACTATTTTAAACTTTATCCTTTCAGCTTTACTTAGTGCGATGTTTT30.29910269192422734No Hit
GGCAGAGAGTGGGGCACAGGCAGGCCCTTGGGTGCTAAGGGCTGGGCTGCATGTGGGTAGCCCGAGCTCCT30.29910269192422734No Hit
TTCCCTACACGACGCGGGAAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29910269192422734No Hit
ATGCTGAACTTAAGGGTTTAGATGTAGATTCTCTGGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAG30.29910269192422734No Hit
GGTTAAGTTAAAATAAAACCAAGGGATATCTTGCATAATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29910269192422734No Hit
GGCTAACACGGTGAAACCCCATCTCTACTAAAAATACAAAAAATTAGCCGAGTGTAGTGGCAGGCGCCTGT30.29910269192422734No Hit
CTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29910269192422734No Hit
GCCTATACAAAGAGGTATCTAGAAGAGCAGAAGACAGAGAATGGAAAAGATAAGGAACAGAAACAAACAAA30.29910269192422734No Hit
GCTGTAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGGAACAAGGACCAGGAGGTGAACTTCC30.29910269192422734No Hit
GTAGTGAGCTGAGATTGTGCCATTGCCCTCCAGCCTGGGCAACAAGAGTGAAACTCCATCACAAAAAAAAA30.29910269192422734No Hit
GTCCAGGAGTCTGTGGCTGCTGTGAACCCGGATTACACCACAGTACTCCAGCCTGGGTGGCAGAGCTAAAC30.29910269192422734No Hit
GTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCT30.29910269192422734No Hit
GATTTTAATTATTGGAATGCACAATTTTTTTAATATGCAAATAAAAAGTTTAAAAACTCAAAAAAAAAAAA30.29910269192422734No Hit
ATGTATGTAGTGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29910269192422734No Hit
ACTCCCAGGAGAAGCCAGCGATGGAGAAATGGAGAGCGCAGGACTTCTCCCCATGTTATGGCTGATCAGTC30.29910269192422734No Hit
GACATGGTAGGATCGCTTGAGCCCAGAGGCTAAGGCTGTAGTAAGCAGTGAGCCATGATTGTGCCACTGTA30.29910269192422734No Hit
TCTGGAGCCTGATGTCTTAACGAATAAAGGTCCCATGCTCCACCCGAAAAAAAAAAAAAAAAAAAAAAAAA30.29910269192422734No Hit
ATTTGAAATAAAGGTATAGTAACCTTAAAAAGAACATTATAACTGATTGTTGTGAATGGGGTGAATTTGTT30.29910269192422734No Hit
GAATTTACATTATACAACACTGAAATGTTTAAGAAAGTCTATGTAGACATCTTTCTCAATTTTCAATTCTC30.29910269192422734No Hit
CTCTTCCTGTTTTGTACATAGATTTATTTTTCAGTTCCAAGAAAGATGAATACATTTTGTTAAAAAAAAAA30.29910269192422734No Hit
GGCATGGTGGCGGGCACCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGA30.29910269192422734No Hit
ACTCAGGAGGCTGAGGTAGGAGAATTGCTTGAACCTGTAAAGGTGGAGGTTGCAGTGAGCTGAGCTCGCAC30.29910269192422734No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCC20.19940179461615154No Hit
GTGTGCTACTAACAGATTATAATAAATTGTCATCAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.19940179461615154No Hit
CTGATGCTGCCTCTGCCTTCTGATGCTGGACTGGCCTTGCTTCTACAAGTATGCTTCTCCCACAGCTGTGG20.19940179461615154No Hit
GGAAGAATGTTTATTCTTCAGTCCAAGGAAGCAATTCACAGTCAGCTGTTAGAGAAGCAGAAAATAGCAAA20.19940179461615154No Hit
GGCATGCACTGGGGGGCTGGAGGAGCTGGGCTGGACCCTTCCCACCTGAGCATGCTGACCCCCTTCCTACC20.19940179461615154No Hit
CGTCCTTGCTGCTTTGCCACCCCATTCCCGTCAATAAAGTGGTTTGAACCCAAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
ATATATTAGTTTTGTTTAAATAATTTCATGCCTTTTGTATCTAGGAAACTTTTTCACATAAATTAAATACA20.19940179461615154No Hit
CAGAGGCAGAGGATGCTGCAAGAGAGAAAAGCTGCAAAAGAGGCCGCCGCTGCCACCTCCTGAGGCAGCTG20.19940179461615154No Hit
TGTGAGAACATTAGGCCCCAGCAACACGTCATTGTGTAAGGAAAAATAAAAGTGCTGCCGTAAAAAAAAAA20.19940179461615154No Hit
GCATTAGAGGGCGGAAGCGCTATCCGAGCAGGATGCGGTTCGTGGTTGCCTTGGTCCTCCTGAACGTCGCC20.19940179461615154No Hit
CTCTACGTTTGGTTCAATTAACCAGTAGGTTACAGTTATTGAAAATTAAAGTACAGTTTAAAGCTCAGAAA20.19940179461615154No Hit
GTTCTGTTTTAAGTAACAGAATTGATAACTGAGCAAGGAAACGTAATTTGGATTATAAAATTCTTGCTTTA20.19940179461615154No Hit
TGTTCATCGTAAACACCAACGTGCCCCGCGCCTCCGTGCCGGACGGGTTCCTCTCCGAGCTCACCCAGCAG20.19940179461615154No Hit
AACGCTCACTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
TACTCGGAGGCTGAAGCAGGAGAATGTCTTGAACTCGGGAGGCAGGTTCAGCAGTTGCAGTGAGCTGAAAT20.19940179461615154No Hit
CCTCCCAGAGCCTTGAGATAGAACTCCAGTCCCATCTCAGCATGAAAGAGTCTTTGGAGCACACTCTAGAG20.19940179461615154No Hit
CTCTTGGATGATGTTTGCCGTCAGCATTCACCAAATAAACTTGCTCTCTGGACAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
TGATACAACTATGCAGATTTTATAAATGTGCAATAAAAGTATTTGTTTTAAAAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
AATGAGGAACAGAATAATCAAGAATGAAGTTAAGAAGCTTCAAAAGGCAGCTCTCCTGAAAGCTTCTCCCA20.19940179461615154No Hit
ACTCAGGAGGCTGAGGTAGGAGAATTGCTTGAACCTGTAAAGGTGGAGGTTGCAGTGAGCTGAGATCGCAC20.19940179461615154No Hit
GCATTATCTTAAATAGTGAAGATGGAGAAATATTCAATAATGAAGAGCATGAATATGCATCGAAAAAAAAA20.19940179461615154No Hit
CCACTGAGGAAGCCACTGCCACCATGGGAGCTACTTCTCGGTTCCCTCCCCGATGGTCTACCTCGAAGGGA20.19940179461615154No Hit
TTGCATGATTCTCCAGTATATTTGTAAAAAATAAAAAAAAACTAAACCCATTAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
CCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC20.19940179461615154No Hit
CACCTCTACCAAGTACACTCGTGATATCTATAAAGAGCTCCTCATCTCCTTAGTGGCCTCACTCTTCATGG20.19940179461615154No Hit
TCCCTACACGACGCGCGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC20.19940179461615154No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGC20.19940179461615154No Hit
GCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGA20.19940179461615154No Hit
GAGCAAGACTTTGTCTCAAAAAAAAAAAAAGAGAGAGACAGAGAAGGAGAGAAAGAGAAAGAAAAGTAGGC20.19940179461615154No Hit
GTTCTGTTTTCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
ATTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTC20.19940179461615154No Hit
AGCTATATGAAAATAATCATAAGAATTAAGAGTTCCAGGCTGGGCGCGGTAGCTCACACCTGTAATCCTAG20.19940179461615154No Hit
GGCTGGACCTGCCCTGTCCTCAGCACGCGTGTGCAAAAGTGAACAATAAATCATTTCAAAGATAAAAAAAA20.19940179461615154No Hit
GCTTAAGTACTTATTGTGGTAGTGAGTCCTACGGTATTTCAGTAAAAAGGAATTCATGGCATAAAAAAAAA20.19940179461615154No Hit
ACTGTGAATATGAGTATGCCAGCTGCATACGATGTAACTAATCATATTTAAATATATTTCACTTTCTCTTT20.19940179461615154No Hit
GACTTGTACAGAATCCTGTTTTGTCACTTCTGCCCACATGTCCCAAATGCCAACAGGACCAAGACCATAAA20.19940179461615154No Hit
ACCATATAATAAATCACCTCTTCCGCTGTTTTAGCTGAAGAATTAAATCAAAAAAAAAAAAAAAAAAAAAA20.19940179461615154No Hit
GGTGGCACGTTCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCATGAGAGAGAATCACTTGAACCCAGGAG20.19940179461615154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)