FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35413
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG1380.38968740293112697No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAG1190.33603478948408777No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG940.2654392454748256No Hit
ATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTA830.23437720611075027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.23155338435037978No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGA780.22025809730889787No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATT730.20613898850704543No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAA710.20049134498630444No Hit
CATTTATACTACTCATTTTTCTTGAGCACCTACTATGAGCTGGGGATCTGCAGATAGAGCAGAAGGATGAA700.19766752322593395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG660.18637223618445203No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAG640.18072459266371108No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTA610.17225312738259962No Hit
GATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTT590.16660548386185864No Hit
TTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAA530.14966255329963574No Hit
ACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGT530.14966255329963574No Hit
TTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGA510.14401490977889475No Hit
GTTTAATATCTATCTTTATAAACAAGCTCCATAAAGTTAGGGACCATATTTCTCTTCTCCAGGTTTGTAAT480.1355434444977833No Hit
GGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGC480.1355434444977833No Hit
GCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGC460.1298958009770423No Hit
CTTTAAAGGATTATAAATAATCCATTTAAAAATTCAAGTACACACATCAGTGTTGGTTACTATGCAGAGAA450.12707197921667185No Hit
ATATATAGTAGTAAGGGTTTTATGAATGTTGATTATTTTGTGCCAACAGCCCAGAATTGTCACTTATATGT440.12424815745630136No Hit
CTTTAATGTTAGTCATTTATTAGAAAGATCCTTTATCCTGATTTGCTTAAACCTTTCAATAAATTGCACTT430.12142433569593088No Hit
AGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGG430.12142433569593088No Hit
AGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAG420.11860051393556038No Hit
GTTGAAAGAAGTACTGATGACTTTTCAAAACAAATGAACCACCGTAGCTGACAGAGAACCGTATCGTAGAG420.11860051393556038No Hit
GTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATAT410.11577669217518989No Hit
GCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGA400.11295287041481943No Hit
TCCTAATATCATGGGGGAAAAAATACCAAATTTAAATTATATGTTTTGTGTTCTCATTTATTATCATTTTT400.11295287041481943No Hit
ATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGG380.10730522689407845No Hit
GTATCTATGTCTCTGAAAAAGGAACTGTTCAGCAGGCTGATGAATAAGATCTAAGAGTTACCTGGCTACAG370.10448140513370796No Hit
TTCTTATACACCACCAACAGACAAACAGAGAGCCAAATCATGAGTGAAGTCCCATTCACAATTGCTTCAAA370.10448140513370796No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCT370.10448140513370796No Hit
GTCTATTTAGTGTGCATGTTTTTCCTTAATGATGTATTTGATCTGACTTTTTTCCTTCTCAAAAGAATCAT360.10165758337333747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)