FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225884
Sequences flagged as poor quality0
Sequence length66
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27891.2347045386127393No Hit
CTCTTGTACATGGCCTTTCTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGG26851.1886632076641108No Hit
GTACATGGCCTTTCTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGG11790.5219493191195481No Hit
GGACAGGGTTGCGGCTGCAATGGGGCTTCCCCTTCTTGGGCTTTTTAGCTTTGAGGTTACCCTTTT9670.42809583680119No Hit
CTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAG8680.3842680313789379No Hit
CTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAA8640.38249721095783673No Hit
GACAAGGACAGGGTTGCGGCTGCAATGGGGCTTCCCCTTCTTGGGCTTTTTAGCTTTGAGGTTACC8120.35947654548352254No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7940.35150785358856756No Hit
CTCTTGTACATGGCCTTTCTGGAATACATGGCAGCTCGGGAATACCTGCCAATTCCTCTGACAAGG7200.3187476757981973No Hit
GTACTTCCTCTTGTACATGGCCTTTCTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCT6660.2948416001133325No Hit
TTCTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGC6340.2806750367445237No Hit
GCCTTTCTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGG5670.25101379469108037No Hit
CTTGTACATGGCCTTTCTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGAC5650.2501283844805298No Hit
ATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAATGGGG5450.24127428237502435No Hit
GCAATGGGGCTTCCCCTTCTTGGGCTTTTTAGCTTTGAGGTTACCCTTTTTCACCTTGCCACCAGC4950.21913902711126065No Hit
CGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAATGGGGCTTCCCCTTCTTG4920.21781091179543483No Hit
GAGTACTTCCTCTTGTACATGGCCTTTCTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCT4430.1961183616369464No Hit
GCTGAGTACTTCCTCTTGTACATGGCCTTTCTGGAATACATGGCAGATCGGGAATACCTGCCAATT3820.16911335021515467No Hit
CCTCTGACAAGGACAGGGTTGCGGCTGCAATGGGGCTTCCCCTTCTTGGGCTTTTTAGCTTTGAGG3800.16822794000460412No Hit
GCCTTGGATTTAGCGGCTGAGTACTTCCTCTTGTACATGGCCTTTCTGGAATACATGGCAGATCGG3590.15893113279382337No Hit
GGGTTGCGGCTGCAATGGGGCTTCCCCTTCTTGGGCTTTTTAGCTTTGAGGTTACCCTTTTTCACC3510.1553894919516212No Hit
ACCTTGGATTTAGCGGCTGAGTACTTCCTCTTGTACATGGCCTTTCTGGAATACATGGCAGATCGG3290.14564997963556514No Hit
GGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAATG3250.14387915921446406No Hit
GGCAAGGACAGGGTTGCGGCTGCAATGGGGCTTCCCCTTCTTGGGCTTTTTAGCTTTGAGGTTACC3210.142108338793363No Hit
CTTCCTCTTGTACATGGCCTTTCTGGAATACATGGCAGATCGGGAATACCTGCCAATTCCTCTGAC3100.13723858263533495No Hit
ACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAATGGGGCT3100.13723858263533495No Hit
GTACATGGCAGATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAATGGGG3080.1363531724247844No Hit
GGATAATATCTAGGCATTTTGCGAAGTTTAACCACCCGGGTACCGCCGTTCTTGTCACCACCAACT2690.11908767331904871No Hit
GTACATGGCCTTTCTGGAATACATGGCAGCTCGGGAATACCTGCCAATTCCTCTGACAAGGACAGG2590.11466062226629598No Hit
GATCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAATGGGGCTTCCCCTTC2520.11156168652936904No Hit
GCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAATGGGGCTTCCCCTTCTTGGGCTTTTTAGC2450.10846275079244214No Hit
CTGGAATACATGGCAGCTCGGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAG2400.10624922526606577No Hit
TTCTTGGGCTTTTTAGCTTTGAGGTTACCCTTTTTCACCTTGCCACCAGCATCAACCTTCTTGGCT2390.10580652016079051No Hit
GGGAATACCTGCCAATTCCTCTGACAAGGACAGGGTTGCGGCTGCAATGGGGCTTCCCCTTCTTGG2310.1022648793185883No Hit
GCCTTGGATTTAGCGGCTGAGTACTTCCTCTTGTACATGGCCTTTCTGGAATACATGGCAGCTCGG2300.10182217421331302No Hit
GGATTTAGCGGCTGAGTACTTCCTCTTGTACATGGCCTTTCTGGAATACATGGCAGATCGGGAATG2280.10093676400276248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)