FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931
Sequences flagged as poor quality0
Sequence length66
%GC20

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83543.24184360435008No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.3625064733298809No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.31071983428275507No Hit
GCTTTCCCGGCTTCTCTGTGAGGGGTCCCACCAGCCCAGGGCTGATCCCAGCTGTGTGTCACCAGC50.25893319523562924No Hit
GGGAGGACCAGATCAACAGGCTTATTAGAAGAATGAACTAAGGTGTCTACCATGATTATTTTTCTA40.20714655618850336No Hit
ACAACAGCCTGGGCAACACGAGCAAAACTCCGTCTCAAATAAATAAATAAATAAATAAATAAATAA40.20714655618850336No Hit
GCTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAAAC40.20714655618850336No Hit
CTCAGTGCAGACAAAAAGTGCATCATTGTAGAAGAAGGCAAAGAGATCTTGGTTGGAGATGTTGGT40.20714655618850336No Hit
GGTCCCATGGCCTGCTGGGCTTGGCTCGGCGCCCCCACCCCTGGCTACCTTGTGGGAATAAACAGA30.15535991714137753No Hit
GCTGTACTGGAGCCACCCGCGAAAATTCGGCCAGGGTTCTCGCTCTTGTCGTGTCTGTTCAAACCG30.15535991714137753No Hit
CTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA30.15535991714137753No Hit
GCATCTAAAGTGAAGACTTAAGAAAAACTTAGTGACTACTAGATTATCCTTAGGACTCTGCATTAA30.15535991714137753No Hit
GCCCTCCATCAAGAGCCCTCAACACAAGAGTTTGTTATAAATAGAAATAAACGACAAAAAGTAGAG30.15535991714137753No Hit
GTATATTCACATCTAATAGGTTAATATGTACATTTGGATGATGATTTTTCTTGATAACATTAACAT30.15535991714137753No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC30.15535991714137753No Hit
GTAGCTTATATCAATTTAGATTTCATCATTACTATTTTGCATACTGGAATTTATAAATGTGTAAAT30.15535991714137753No Hit
GGGACCAGAGCTGTGTGCCACCACACCCAGCTAATCTTTTCTTTTTGTTGTTGTTAAAATGGGGTA30.15535991714137753No Hit
CTCCCAAAGTGCTGGGATTACAGACGTGAGCCACCGCGCCTGGCCAAAAAAAACCTTTTTTTAAAA30.15535991714137753No Hit
GCACTCCTTTGGTTATGAAGCCGCTGCCCCCAACACCTGTGGAATTTGTCTTCAGGGTTCTCCTTC30.15535991714137753No Hit
GGGACCCACCAGGGTGCAGCTGGGCTCCAGGGTCCAGCCCACAAGCTGCATCAGGGTCTCTGGGAG30.15535991714137753No Hit
GCGGTGCAGCGTGTGTGCCACCTCATTATTATCTAGCTAAGCGGAACATGTGCTTCATCTGTGGGA30.15535991714137753No Hit
CCGGCGGGGCCTCCCGGGAGGAGAACGGGGAGGTGAAGCCGCTGCCCCGAGATAAAATCAAAGACA30.15535991714137753No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15535991714137753No Hit
CTATTAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GGTGCGGTGGCTCATACCTGTAATCCCAGCACTTTGGGAGGCCGAGAAGGGTGGATCACCTGAGGT20.10357327809425168No Hit
GGGGAGTATTGGCCCTGGCGGAACGCAGCAACCACGAGGGATCCAGCCAGAGGATGCCACGCCAGC20.10357327809425168No Hit
GGGTCACCCTGCCACACTTGTTTCCTAGTGTTTCTGTGGCAGACATTGCTAATCAATTACAGCCCT20.10357327809425168No Hit
ACTCAAACTCCTTTAGTTTTGTAATTCAACCTCATAAGCTGTCCAATAAGCTAAATGAAAACTAAG20.10357327809425168No Hit
TCCTAATATCATGGGGGAAAAAATACCAAATTTAAATTATATGTTTTGTGTTCTCATTTATTATCA20.10357327809425168No Hit
CCCACCACCACAATAAAAATCACCCCCACCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
ATTTAAGTGCGTGTGTAGAGTTAAGAAGCTGTTGTACATTTATGATTTAATAAAATAATTCTAAAG20.10357327809425168No Hit
CTATAATCGTAAACACATGTATTTGAAAACAAAACAAAAAAAGTACTGAAAAACTGATATGATAGG20.10357327809425168No Hit
GGTGATGAGGAAGATGAAGAGGAGAGTGATGCTGTGGTGTCCCAGGTTCTGGATGAGCTGGGACTT20.10357327809425168No Hit
AGGCCGAGGTTGCGGTGAGCCAAGATCGAGCCATTGCACTCCAGCCTGGGCAACAAAAGCGAAACT20.10357327809425168No Hit
GTAATAAATATGTAAGGCCTTTCTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GCTCAGGCAGCTCGAGGCCCCCGACCAACACTTGCAGGGGTCCCTGCTAGTTAGCGCCCCACCGCC20.10357327809425168No Hit
GAACTAATCGCTGATCGTCAGATCAAATAAAGTTATAAAATTGCAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GTCTTGGCTGTGTCTTGCCTTTTAATCATGAACAGTTACATGATTTATAATTTCACTGATTGAGAT20.10357327809425168No Hit
ACAGGTGCAAACTACTGTGCCTGGATGACATGAATAAACTCTGGAGTAGATTTTAAAAGGAGAAGG20.10357327809425168No Hit
CCCCCTGCTGGTGTCTAGTGTTTTTTTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGG20.10357327809425168No Hit
GAATACAGACGAGTCCAACCATGACCCTCAGTTTGAGCCAATAGTTTCTCTTCCTGAGCAAGAAAA20.10357327809425168No Hit
GAAGAATTGCTTGAACACAGGAGGCAGAGGTTGCAGTGAGCTGAGATCGCGCCACTGCACTCCAGC20.10357327809425168No Hit
CCTGTAGTCCTAGCTACTTGGGAGGCTAAGGTGGGAGGTGGCTGAGGTGGGAGGATCACTTGAGCC20.10357327809425168No Hit
ACTTAACTGTGCTGTGCCCCATTTCCTATGAAATAAAACAACCTAATTCATAAAAAAAAAAAAAAA20.10357327809425168No Hit
TTCGTGCACTCTGCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTTTACA20.10357327809425168No Hit
GTTGTCATTCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
CAAATATGAAACAAATGTGTTTTATTTCATGGGAGTAAAATATACTGCATACAAAAAAAAAAAAAA20.10357327809425168No Hit
GTGTCAGTGCTAATGAAACATGTTGGTTGGTTTCTAAAATAAAGCCAAACAAGCCAGCACATGCCG20.10357327809425168No Hit
ATGCTAAACTTTTGGCCAAGAGAATGAAGGAGGCTAAGGAGAAGCGCCAGGAACAAATTGCGAAGA20.10357327809425168No Hit
GTTTTGTAAATGGAAATACCACTACTAAAGACAGAATGCTATAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
TAAATATTCCTTTGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
CTACACGACAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GTATTATGGCAACTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
ACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAGGG20.10357327809425168No Hit
GGATACATTCCTGAACACATACAACCTACCAAGACTGAATTATGGAGAAATGGGAAAGCTTAACAA20.10357327809425168No Hit
GTGGAAGATGAAATGAAAGCTATGGGACTCTTGGAGGATACCCAGTGTCTATTCTGGGTTAGAGAA20.10357327809425168No Hit
ACATAATGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTGGGTGCGGTGGCGGGCTCCTGTA20.10357327809425168No Hit
GGCACAGACCCGGGCAGAGGGACAGCGCCAGGCCCAGGAGTATGAGGCCCTGCTGAACATCAAGGT20.10357327809425168No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGAAATAAAGCTTTTTGAT20.10357327809425168No Hit
CCTCAAACCAGCCCAACCCGAGCTTTTGGACAGCTCTCCAGAAGCATGAACTGCAGTGGAGATGAA20.10357327809425168No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATA20.10357327809425168No Hit
GGCGGGGTAGCTCACACCTGTAATTCTAGCACTTTGGGAGGCTGAGGTGGGCAGATCACTTGAGGC20.10357327809425168No Hit
GCACGCCAAGTACACTTGCTCTTTCTGTGGCAAAACCAAGATGAAGAGACGAGCTGTGGGGATCTG20.10357327809425168No Hit
AAAATAAACTTTGTAAATTAACTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit
GAGTTTGGACTGCCCGTCAGATTGTTGCTGCACATAGTTGCCTTTGTATCTCTGTATGAAATAAAA20.10357327809425168No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10357327809425168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)