FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18347
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2531.3789720390254536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1170.6377064370196762No Hit
CTTATAAGACTCTTCCATCAGTTTCAGAAACGTTTCCAACGTTAAGATCGATGATTGAGGTGCTAAACACA440.2398212241783398No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAG410.22346977707527116No Hit
GTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGG390.2125688123398921No Hit
CATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTCT340.18531640050144438No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG340.18531640050144438No Hit
GTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAA330.17986591813375485No Hit
GTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAAGAGAATTAATGTGCGTATTGAGCACATTAAGCA320.1744154357660653No Hit
GTACCTGGGTTCAACTAAAGCGCCAGCCTGCTCCACCCAGAGAAGCACACTTTGTGAGAACCAATGGGAAG310.16896495339837575No Hit
GCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGA290.15806398866299667No Hit
GTCCCGAACCCCTGGTAATAGAATTGTTTACCTTTATACCAAGAAGGTTGGGAAAGCACCAAAATCTGCAT290.15806398866299667No Hit
GTGTGTACTTAGTTGCTGAGGAGAAAAACAATACAGGGCACAATTTCACGAAACTATTAGGTTTTAAGCTG280.15261350629530715No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT280.15261350629530715No Hit
GATCAGGACACTTAGCAAATGTAAAAATAAAATCTAACTCTCATTTGACAAGCAGAGAAAGAAAAGTTAAA270.1471630239276176No Hit
AGGATAATGTGCATTGAGAATATTTTGAAAAATAATTGACTCAAATTTTATTTCTTGGTCTTTTGCTGTTT250.13626205919223852No Hit
AACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAAC250.13626205919223852No Hit
CTATAATGGGATTCATTGGCTTCTTTGTGAAATTGATCCATATTCCTATTAATAACATCATTGCTGGTGGC250.13626205919223852No Hit
ACAAAGAAGATTGTGCTAAGGCTTGAGTGCGTTGTGCCCAACTGCAGATCTAAGAGAATGCTGGCTATTAA250.13626205919223852No Hit
CATATATGCGAATCTATAAGAAAGGTGATATTGTAGACATCAAGGGAATGGGTACTGTTCAAAAAGGAATG250.13626205919223852No Hit
GTGTTTATGTCATGCCAATTGTATAAACATGATCTTATATATTTGTGATTTTTTTTCTCCCTCCCAGATAG240.13081157682454897No Hit
GGATTATTGGAAATTTGTTATAATGAATGAAACATTTTGTCATATAAGATTCATATTTACTTCTTATACAT240.13081157682454897No Hit
AGAATAATCTACGTGACTGGTTGCGCGTAGTTGCTAACAGCAAAGAGAGTTACGAATTACGTTACTTCCAG240.13081157682454897No Hit
GATTCGCAAACTTCCCTTCCAGCGTCTGGTGCGAGAAATTGCTCAGGACTTTAAAACAGATCTGCGCTTCC230.12536109445685945No Hit
ACTTCATGGATGTGAAATGCCCAGGATGCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTT230.12536109445685945No Hit
CACTTCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACCAACCTCCACAGGCTGGG230.12536109445685945No Hit
TCCCACTGATGGCTCAGGGCCATCGATGCACCCCTCGGAGCTAATAGCGGAGATGAGAAACAGTGGGTTTG230.12536109445685945No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCG220.1199106120891699No Hit
GGTTTTTCTTTCACTACTTTACATTCTTGGATTTTGTTGTTTTGCTGAGTGATTTGGGAGTTGAGGTGAGT220.1199106120891699No Hit
GTATTGAAACTTTTAAACAATACTGAAGAAAAAAAAAACTTTTCCGACATCTGTTCTTGGTCTTTTGTGAC220.1199106120891699No Hit
GTGACAAACACTCCTTTGGCCTGTTGAATTTGCTGAAGAACATCACCTAAAGTCTGCACACGAGCCCATTT220.1199106120891699No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGA220.1199106120891699No Hit
AAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGAT220.1199106120891699No Hit
GTCCAAAACAAAGAAACATGTCAGCAGGGCCTATGGTGGTTCCATGTGTGCTAAATGTGTTCGTGACAGGA220.1199106120891699No Hit
CCATAAAGCTATCAGAAGAAATCCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGGGAGATGC210.11446012972148034No Hit
GTTTATGTCATGCCAATTGTATAAACATGATCTTATATATTTGTGATTTTTTTTCTCCCTCCCAGATAGGA210.11446012972148034No Hit
CTCCAGATGTGCGCATTGACACCAGGCTCAACAAAGCTGTCTGGGCCAAAGGAATAAGGAATGTGCCATAC210.11446012972148034No Hit
GTTTAAGCCTGAAGAACTGGTTGACTACAAGTCTTGTGCTCATGACTGGGTCTATGAATAAGAGGTGGACA200.10900964735379082No Hit
ACATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTC200.10900964735379082No Hit
GTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAAGAAA200.10900964735379082No Hit
TTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC200.10900964735379082No Hit
CACCCATAGTAGGCCTAAAGGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAA200.10900964735379082No Hit
GTGCAAACACATCTTCTGTTAAACCCATTGGTATACACAGAATATTCCTGTGCCCACACTTAATGTCAATC200.10900964735379082No Hit
GAGTATGTGATTGGTAGTAAGAGACACACAGAATGTTTATGAAGAAATTGCATTTTCTTTTCCTTTACATT200.10900964735379082No Hit
AGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAAT200.10900964735379082No Hit
CACACAAATATTGCTTGGACTACCTATAAATGGACTTGCTTCTTGTGTTTGAAACACTCGTGTGCATGAGA190.10355916498610127No Hit
GATCAGGACATCCCGATGGTGCAGCCACTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATC190.10355916498610127No Hit
CTCAAGCCCAGAAAGATGAATTAATCCTTGAAGGAAATGACATTGAGCTTGTTTCAAATTCAGCGGCTTTG190.10355916498610127No Hit
CTCTGAATCTGTTATGAACACGTTGGTTGGCTGGATTCAGTAATAAATATGTAAGGCCTTTCTTTTTAAAA190.10355916498610127No Hit
GTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCT190.10355916498610127No Hit
TACCGAATCCGTGTGCGGCTGTCCAGAAAACGTAATGAGGATGAAGATTCACCAAATAAGCTATATACTTT190.10355916498610127No Hit
GTGGTTATCACTAGGCTAAAACTGGACAAAGACCGCAAAAAGATCCTCGAACGGAAAGCCAAATCTCGCCA190.10355916498610127No Hit
GTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGA190.10355916498610127No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGC190.10355916498610127No Hit
ATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTATGTACTTAGCTGTACTATAAGTAGTTGGTTCGTAT190.10355916498610127No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATT190.10355916498610127No Hit
CATGGAACCTAGCTACTTCCTGGGAGGTGGGGCGGGGACTACGGAGAAGGTGCAGCCAGGCTGTGGCAAAG190.10355916498610127No Hit
GAGTGAGAACTTTCCCTACCGTGTTTGATGAATGTTGTCCAGGTTCTATTGCCAAGAATGTGTTGTCCAAA190.10355916498610127No Hit
CAAAAAGATATATAAACTCTCTTCACAAGAAGAAAGTATCGGGACATTATTGGATGCTATCATTTGTAGAA190.10355916498610127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)