FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11694
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10989.389430477167778No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1361.1629895672994697No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT940.8038310244569864No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA650.555840602018129No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG530.4532238754917052No Hit
GTTCTGGTTGCTGGATTTGGTCGCAAAGGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGT390.33350436121087734No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCACTATTAAAGGTTCGTTTGTTCAA390.33350436121087734No Hit
CATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTCT380.3249529673336754No Hit
GAACTGTGATGTGATGTGGAAATACTGATGTTTCCAGTAAGGGAATATTGGTGAGCTGCATATATAAATTT320.2736446040704635No Hit
GCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTA270.23088763468445359No Hit
GGTTCCAGGACATAGTCTGAGGCAAGATGGAGGGTATGAGGGGCCTTCACACTTCACTTCATCCCTTCTAC260.2223362408072516No Hit
GCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAG250.2137848469300496No Hit
GAGTTATACTCTGTTTGCTACATCATAATGCTGGTTTTGCTGACTTTTTGTTTTTTTATATATTTATAAAA240.2052334530528476No Hit
GTCCTGCCCCCTCCACTGCTGCTGCTCCAGCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAG240.2052334530528476No Hit
GGCGTCTTGCAATCTCTGAAGATCATGTGGCCTCAGTGAAAAAATCAGTCTCAAGTAAAGGCCAACCAAGC230.1966820591756456No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC220.18813066529844363No Hit
GGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGATGC220.18813066529844363No Hit
GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGGCC220.18813066529844363No Hit
AACGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAG220.18813066529844363No Hit
GTAGGGGCCGGTGGACCTGCTCCAGCAGCTGGTGCTGCACCAGCAGGAGGTCCTGCCCCCTCCACTGCTGC220.18813066529844363No Hit
ACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAA210.17957927142124167No Hit
GAGCATTGGATTTGCTAAAGGAGCTTAAGAATATTCCTATGACCCTGGAATTACTGCAGTCCACAAGAATC210.17957927142124167No Hit
GCCATCAACGAAGTGGTAACCCGAGAATACACCATCAACATTCACAAGCGCATCCATGGAGTGGGCTTCAA210.17957927142124167No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA200.17102787754403967No Hit
GAGGGACTGGAGGCCAGCGGTGTCCACCCTTGCCCTCAGCAAGAGAGAATGCATTCTTAAAAGAAAGCTGT200.17102787754403967No Hit
TTCGTAGGATGGGTGAGTCAGATGATTCCATTCTCCGATTGGCCAAGGCCGATGGCATCGTCTCAAAGAAC200.17102787754403967No Hit
GACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGG200.17102787754403967No Hit
AACTGGATCCTGGCAGCGAGGAGACCCAGACGCTGGTTCGAGAGTACTTTTCCTGGGAGGGGGCCTTCCAG200.17102787754403967No Hit
CGTCTACGTTGAATTATTCAGAGAATTAAGCAATAAAAGCTCACACCTTATTGTCAACAGTGTTTTTATTT200.17102787754403967No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.17102787754403967No Hit
GCCTTTTGCCCGCTCTTGCTCCAGAAAAAACAAAACAAGGATCTGTTAGCTCTTTAAAAGAAGAAAAACAC200.17102787754403967No Hit
GCTGTCCACGGAGATGCCTCGGCAGTGGAATCCAATTTCTTTCCAGCCACAACCTACCCCATGGGTCGACC190.1624764836668377No Hit
CCCTCAGTTGCTGCTCCGACGTGGACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTACGAGTG190.1624764836668377No Hit
CTTCGAGGGGTTCGTGCTGTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGCAG190.1624764836668377No Hit
GGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCACTATTAAAGGTTCGTTTGTTCAAC190.1624764836668377No Hit
GGGCATTGCTATTTATTCCTCGTCGGGCTCCCTTTGACCTTTTTGAGAACAAGAAGAAAAAGAACAACATC190.1624764836668377No Hit
AGACTGGGCTTTGGACAATCTAGAGGTAATTTATATCCGCCCCAGGTGGAGCAACATGCGATTCTGGAGGC190.1624764836668377No Hit
GACCTGTCAGGCTACATGGAGTATTACTTGTATATTCCCAAGAAAATGTCCCACATGGCAGAAATGATGTA190.1624764836668377No Hit
GCTGTATTGACTTGTTCAAGAATAACTGATATGCCTTCACTCAGAAGAAAAGAAATGAATGTGAAAGAAAG190.1624764836668377No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC180.1539250897896357No Hit
GTTTTATTGTGTTTTTAATTTATTTATTAAGATGGATTCTCAGATATTTATATTTTTATTTTATTTTTTTC180.1539250897896357No Hit
GCGTCTTGCCACCGGCTTCCCTTTGTAAATTGAGCTCTGCACGCTGGGGAGTTTGTTGGGTGAACTTTGAG180.1539250897896357No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGA180.1539250897896357No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATT180.1539250897896357No Hit
CCCAAAGTACTGGGATTACAGGCGTGAGCCACCGCGCCCAGCCAGAGTCAAATGATTTCTCTAAAAGCCAA180.1539250897896357No Hit
GGATTTAAGAAAGATTGGTTGCAAGCAGATATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGC170.1453736959124337No Hit
GCGCTACAATTACCTAAAACCTCGGGCAGAAGAGGAGAGGAGGATAGCAGCAGAAGAGAAGAAGAAGCAGG170.1453736959124337No Hit
GCCTCCTGCCCAGGCTCCGGACATGGACATCTTCCAGCAACAGATCTCGAGAAGACAGCTGGCTAAAATCC170.1453736959124337No Hit
CATACAAATTTTTTGAGGTTATCCTCATTGATCCATTCCATAAAGCTATCAGAAGAAATCCTGACACCCAG170.1453736959124337No Hit
GTGTGAATAAATGCCCAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.1453736959124337No Hit
GGAAACAGGTGCTGAGCCCAAGAGACTCTGGTACCCACTGCTGGCTCATGAAGGAAGAATTATTCCTTATA170.1453736959124337No Hit
GAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAG170.1453736959124337No Hit
CTGTATTCAGAACACACTTGGCAGATGGAGGAAGCATCTGAGTTTGAGACCATGGCTGTTACAGGGATCAT160.13682230203523174No Hit
GCCCAGCGAAGGAGTTCTTTCCATAATTCAAGCATGAGATGATGGTGTCCTGGACTAAGGTGGTAGCAATG160.13682230203523174No Hit
GCCATTTGAAAATATTTTGTCCAAAGATCTATGGGCAGAAGTAATATATGCACTTCTAGGCCTGGTTTCTG160.13682230203523174No Hit
ATTCAGCGGCTTTGATTCAGCAAGCCACAACAGTTAAAAACAAGGATATCAGGAAATTTTTGGATGGTATC160.13682230203523174No Hit
GGAGATCGAGACCATCCTGGCCAACATGGTGAAATCCCGTCTTTATTAAAAATACAAAAAAATTAGCTGGG160.13682230203523174No Hit
GTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGTACAATGTACACA160.13682230203523174No Hit
GGCTTTGTAAATTTAAATATGTAAGTGCTATATAAGTCAGGAGCAGCTGTCTTTTTAAAATGTCTCGGCTA160.13682230203523174No Hit
GTATTGCTCTGAAGAAGCAGCGTACCAAGAAAAATAAAGAAGAGGCTGCAGAATATGCTAAACTTTTGGCC160.13682230203523174No Hit
ATTTAAAAACTTTTTTGTGCCTGTAGTCCTTGCTACTCGGGAGGCTGACGTGGGACGGTCGCTTGAGCCTG150.12827090815802974No Hit
GGGCTTTGGCCATCAGAGGAGGATGTTTTCTCCCCAAGACTTGGAAAAATAACAGCAAGGAAAACAGTGGT150.12827090815802974No Hit
CAGTCTCTGAGAAGTACTTTACCTGAAAGACAGTATGAGAAAAATATTCAAGTAACACTTTAAAACCAGTT150.12827090815802974No Hit
GGTTGTCAATGGCCTCATGCTGGGACAGGCCAGGATTCACGTAAATCGCCTGGAGCAAGCTGTTGTAAATT150.12827090815802974No Hit
GATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTA140.11971951428082776No Hit
ATCTATACTGTTGGAAAACGCTTCAAAGAGGCAAATAACTTCCTGTGGCCCTTCAAATTGTCTTCTCCACG140.11971951428082776No Hit
TCTTGAGGCTGAGGGCGGGTGGTACAGATGTGTATGGGAAACCCCAACCCCTATATATTGTAAATAGATGG140.11971951428082776No Hit
CATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGATGGG140.11971951428082776No Hit
GATAAATAGAACTATCTGCATTATCTATGCAGCATGGGGTTTTTATTATTTTTACCTAAAGACGTCTCTTT140.11971951428082776No Hit
GCTGCCATACGTGTTCAGTGTGAATAGTGTTTAAGTTGAAAATATTGTAAAAAAATTATATTTTTTCAAAA140.11971951428082776No Hit
ACCTTCAGTGTTCGAATCATTGACAAAAATGGCATCCATGACCTGGATAACATTTCCTTCCCCAAACAGGG140.11971951428082776No Hit
CTTGATAGCCTCTTAAGGAAAATTTGCTTCCAAATTTTAAGCTGGAAAGTCACTGGAATAACTTTAAAAAA140.11971951428082776No Hit
ATGTTAGAAGCCTTGGAACGAGTATAAATAATGGCTGTTCAGCAGAGAAACCCATGTCCTCTCTCCATAGG140.11971951428082776No Hit
CCCCCCCAATCGGACTGCCAAATTCTCCGGTTTGCCCCGGGATATTATAGAAAATTATTTGTATGAATAAT140.11971951428082776No Hit
ACTTTCAAAAACATCAAGTCTGTGACCGAACTCAACGGCGACATAATCACCAATACCATGACATTGGGTGA140.11971951428082776No Hit
CAGGACTTAAGACAAATGGGACTGATGTCATCTTGAGCTCTTCATTTATTTTGACTGTGATTTATTTGGAG140.11971951428082776No Hit
CATCCTCTCAAAAGCATGCAAGTGCTTGAAATTTAAATATTTCCCAGATCTAAAACAACTTGTGACTACCT140.11971951428082776No Hit
GCTTTCTGCGATGTGCGTGTCTGTTAGAATAGGCTCTCTACCCAGCTAGAACACCTTCCAGACACTTGCTG140.11971951428082776No Hit
AGGACTTAAGACAAATGGGACTGATGTCATCTTGAGCTCTTCATTTATTTTGACTGTGATTTATTTGGAGT140.11971951428082776No Hit
GGATGGTTGAAATGGAAATGAGCCCCGATCCGTCTTTTGGGGTCAAAGGGGTCATCCTGTCAGCTGAACTC140.11971951428082776No Hit
GACCGAGATGAATCCTCACCATATGCTGCTATGTTGGCTGCCCAGGATGTGGCCCAGAGGTGCAAGGAGCT140.11971951428082776No Hit
CTCTCTGTTGATGGTGAAGATGAAAATGAGGGAGAAGATTATGCCGAATAGACCTCTACATCCTGTGCTTT140.11971951428082776No Hit
GAAAAAGAAAGCAGCAGAGAAAAAAGGGAGCGGTCTCGTAGCCCAAGAAGACGCAAATCCAGATCTCCTTC140.11971951428082776No Hit
GAATCCGCAAACTTTTCAATCTCTCTAAAGAAGATGATGTCCGCCAGTATGTTGTAAGAAAGCCCTTAAAT140.11971951428082776No Hit
AGGTTCTTCCCTTTTACATACTGCTGTCTATGTGGCTGTATCTTGTTTTTCCACTACTGCTACCGCAACTA130.1111681204036258No Hit
GAATATGCTAAACTTTTGGCCAAGAGAATGAAGGAGGCTAAGGAGAAGCGCCAGGAACAAATTGCGAAGAG130.1111681204036258No Hit
GCATTCACAAGAAGGTGGCCCGGACCATCGGCATTTCTGTGGATCCGAGGAGGCGGAACAAGTCCACGGAG130.1111681204036258No Hit
GCGGAAGCGGAGAGCAACAGCGCGCCGGTAACAACCAGCCCCGTATCCCCTCCTTCTGTACCTCAAGCTAG130.1111681204036258No Hit
GTAAAGAAAATCATACAACTCAGCATCCAGTTGGCTTTTTAAGAATTCTGTACTTCCAATTTATAATGAAT130.1111681204036258No Hit
GGTCCGGAAGCAAATCGAGTCCATATAGCTACATTCCACCCTTGTATCCTGGGTCTTAGAGACCCTATCTC130.1111681204036258No Hit
GGTTTGTGAGAGCGAGGCTGAGCCTACAGATGAACTCTTTCTGGCCTGCCTTCGTTAACTGTGTATGTACA130.1111681204036258No Hit
AAAAAATCCAACGGGTGCATTTTCATTCATATGAAAGACTTCTCATAGTACTTTTTTTTCCTTTTTTTAAA130.1111681204036258No Hit
GGATCATGTGTTTAAAAAAAGAAAGGCTACGATGACTGGGCAAGAAGAAAGATGGGAAACTGAATAAAGCA130.1111681204036258No Hit
GCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGTGGAGAAGAAAATTGACAAATACACAGAGGTCCTCA130.1111681204036258No Hit
CCACTGCTGCTGCTCCAGCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGAT130.1111681204036258No Hit
GCCCTGTCCTGTGGCTGTGATGATGGAAATGTTCCCTGTGTTGTTCAGTATGGTAGTCACTGGCCACAAGT130.1111681204036258No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG130.1111681204036258No Hit
CGTGAAGAGGTCTGGCCGCTACCACTTTGTGCCTGGAACCACAGCTGTCCTGACCCAGACAGTGAAGCCAC120.1026167265264238No Hit
GAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAAATCTAGAGTA120.1026167265264238No Hit
GGTGAGAGGCTCTGCTTCCTGCTGCCGCTCTGCAGCCTGGACCTGTGGACCCTGGTTGTAAAGAGTAAATT120.1026167265264238No Hit
CTTGCCCGGCTGTGATTGCTGCCAGGCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGC120.1026167265264238No Hit
GGGCCGGAGACGCTGCAGACCCGCGACCCGGAGCAGCTCGGAGGCGGTGAATAATAGCTCTTCAAGTCTGC120.1026167265264238No Hit
GTGTGGGCATAGGATTTTTTATGGAAGGATAAAATTTGAGAGTTAAGCTAATTCCTGTTCTGGAATAGTCT120.1026167265264238No Hit
ATTCCACGATGCCAAATTCATGGCAGACATTGATCTGGATCCAGGCTGTACATTGAATAAAAAGATTCGAA120.1026167265264238No Hit
GGTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATT120.1026167265264238No Hit
GGATGTGTTAATGGGGTGACTTTGTAGCATGGTATGTTGTCTTGAGTTAACTGTAGTGGGTGGGGAGGTCC120.1026167265264238No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC120.1026167265264238No Hit
TCCCCAATGCAGAAATCTGAAATGCTTCAAAATCCCAAACGTTTTGCATGCCTGCATGATGTTCAGAGGCC120.1026167265264238No Hit
ATGATGATTTTGATGATGAGGAAGCTGAAGAAAAAGCGCCAGTGAAGAAATCTATACGAGATACTCCAGCC120.1026167265264238No Hit
GACTTCGAGGGGTTCGTGCTGTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGC120.1026167265264238No Hit
GGCAGGGGTGGTGGTGCAAAGTTATCAGTAACTCTGGGTTTCTCTAAGCACTTGGTGCTGTCAGAGATGCT120.1026167265264238No Hit
CCTCCAAAATATAGGGAACTATTGGCTAAAAAGGAAGGGATCACAGGGCCTCCTGCAGACTCTTCGAAACC120.1026167265264238No Hit
AATTTGGAGCAGAGTGAACAGCATATGCAAAGGTGTGGAAATGTGGAAATGCATGGCCTGTTAAAGCTGCC120.1026167265264238No Hit
GCAGTATTATTGTCTTTGTGGAGTTGACTAATGATAATTTAAAAATCCTGTAATGGATTTCTACTAAAATA120.1026167265264238No Hit
GGATGGACTCTTGCACATCACTACCTGCAGTTTCGTGGCTCCCTGGAACAGCCTGAGCTTAGCTCAGCGCC120.1026167265264238No Hit
GAGGAACGGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC120.1026167265264238No Hit
GGTCGGCAAATGCATTGGCCTCCTGGTTAAGCACGTTTTTGGGGATAATTTTCTCTTCTTTAGGCAATGAT120.1026167265264238No Hit
GTTGCATAGGTCTCCCTCTCTGGGCACCAGGACTCAGTCCAGCCCAAGACTACTCTGGGCAGCTCCCATCC120.1026167265264238No Hit
CAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGATGCTATAAAA120.1026167265264238No Hit
GCTCCAGGGCTACCTGCGATCTGTGTTTGCTCTGACGAATGGAATTTATCCTCACAAATTGGTGTTCTAAA120.1026167265264238No Hit
GGGGCTGAAAATGGTGGAAAAGGACCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGG120.1026167265264238No Hit
GGCTTTACTTTTTTTAACTGGTGCAGTGGGAAAATAGGACATTACATACTGAATTGGGTCCTTGTCATTTC120.1026167265264238No Hit
GGTCGTAAGGGCTGAGGATTTTTGGTCCGCACGCTCCTGCTCCTGACTCACCGCTGTTCGCTCTCGCCGAG120.1026167265264238No Hit
GGTCCACGTTTGCATCTCCTTCGAAATTATAAACTCCTGGGAGTGCAGGGATGTGTCTCATACATTCTTCC120.1026167265264238No Hit
GATTCACCCAGGTTCAGACACCTCACATTCAACTAGATCACATTTCTTATAATTGTGATGTTAGAAAGTCT120.1026167265264238No Hit
CCTGCAAGTGAAACATTTGAAACGATTATACTTTCTACATAAGACATGGTTGGGACATCAGATACTTACAA120.1026167265264238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)