FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305
Sequences flagged as poor quality0
Sequence length71
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46320.086767895878523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG241.0412147505422993No Hit
GTGGTATAAACAAGAAAAATCCAACAATACATTTGGATGCACCAGAAATACATTAGCACCCTCCCAAATCC170.737527114967462No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG150.6507592190889371No Hit
GTGCCTTAAGAAACAAAGCCCTGTCCTAATTTATCTAGCTTGTCAGTCCGGTCTTAGAGATACCCTCTTTC150.6507592190889371No Hit
GGTACAGGGATTACAGCATTACCAGCCCAGTTCTGTGCCAGGTGCTGAAACTAGTTTTGTGGTAAATACAG140.6073752711496746No Hit
GTGTATCTTGTGAAAATATCCTTCAAGCATGAAGAAGAAATAAAGACTGTCAGACAAACAAAAGCTGAGGG120.5206073752711496No Hit
CCTGGTATAGTCTGTTAAAAGAAAGACTTCAGCCAAATTTATTTAAAGTGTTTAATTGAGCAAAGAAAATT120.5206073752711496No Hit
GGAACAGCTCTTTGGACACTAGGAGAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT100.43383947939262474No Hit
GGTCTTGAACTCCTGACCTCAAGTGATCCTCCCACCTCAGCCTCCCAAAGCGCTAGGATTACGGCCATGAG100.43383947939262474No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT90.39045553145336226No Hit
GGAGGAGGTTGATGTAATGGTGACTCTTTTCTGATCAGCACCTTGGCCGTGATTCCCAAGGTCCCAGCCAA90.39045553145336226No Hit
GCTTAAAGTTGTGTAATACTAAAATCACGAGAACACCTAAACAACAACCAAAAATCTATTGTGGTATGCAC90.39045553145336226No Hit
GAAGGTTAAACAGAAAGGAAAAGCAAATAAGATCATGAGCTTACAACTAAAGCAATTGTCTAACTAATTTG90.39045553145336226No Hit
TCCTGTGGGTAGATCAGTGGATGAGACACTACGTTTGGTTCAAGCATTCCAGTACACTGACAAACACGGAG80.3470715835140998No Hit
CTTGGGCAGGGGAGCATGTGGCTTTTAGAAAAAGTAAAATTATGTGAAATTTTCTGGTTACATGGAAAAAA80.3470715835140998No Hit
GTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAAGAGAATTAATGTGCGTATTGAGCACATTAAGCA80.3470715835140998No Hit
CTGTAAATGCTCAATAAATATTGATGATGGGAGGCAGTGAGTCTTGATGATAAGGGTGAGAAACTGAAATC80.3470715835140998No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA70.3036876355748373No Hit
GACCGAGCTGCGGCTGCTGTGGAACCTGATGTTGTTGTTAAACGACAAGAAGCTTTAGCAGCTGCTCGACT70.3036876355748373No Hit
GAGTGTAGTCTCAGCTTAAAGTTGTGTAATACTAAAATCACGAGAACACCTAAACAACAACCAAAAATCTA70.3036876355748373No Hit
GGTGGTAGTCATAACATAGTTCATGACATTATCTATGTGAGATCAAGAAGGCAGTAGCATCAAAACATGCA70.3036876355748373No Hit
GTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTTCAGTCATGGCATTCGCAGTGCCC70.3036876355748373No Hit
ACCACGCTGGTGCCCACCGCCTGGCCAGCCAAGCCCTGCCGATCAGACATGTGGGCTCCCCGAAGCCCAGC70.3036876355748373No Hit
GATGTTTAGGAATGCTCTTCAGATGTGAAATTTTCTTTTTGTTTTTGCTTTTTGGCTCGTAAATTGGATAT70.3036876355748373No Hit
GGAGTTCAAAACCAGCCTGGCCAACATGGCGAAACTGTCTCTACTAAAAATACAAAAAAATAGCCAGGCGT70.3036876355748373No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC60.2603036876355748No Hit
GCTGGTGCAGACGAAGCGGCGGGCTCTGGAGAAGATTGACCTTAAGTTCATTGACACCACCTCCAAGTTTG60.2603036876355748No Hit
GCTATGACGTGTCGGACTCAGGCCAGCTGGAACATGTGCAGCCCTGGAGTGTCTGACCCAGTCCCGCCTGC60.2603036876355748No Hit
GTTTGCTTTGTCCACAATTTCCTTAAGACCTCTTCAGAAAGGGATTTGTTTGCCTTAATGAATACTGTTGG60.2603036876355748No Hit
CATAGAAAATACTTAAAAAATACTAAGTTAATATTTTTAAAAATATATTTATGTATATACATGTGTAGATG60.2603036876355748No Hit
TTCGTAGGATGGGTGAGTCAGATGATTCCATTCTCCGATTGGCCAAGGCCGATGGCATCGTCTCAAAGAAC60.2603036876355748No Hit
TGTATAAAGTGTGTTTGAACATTATGCCAAATATCAAGATGTGAAGGACTAATTCAGGATGCAAAAACGTT60.2603036876355748No Hit
TACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT60.2603036876355748No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.2603036876355748No Hit
TTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGATGGGAG50.21691973969631237No Hit
ATATATAAAAACTTTGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.21691973969631237No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA50.21691973969631237No Hit
GTAATCCCAGCTACTTGGGAGGCTGAGGCATGAGAATTGCTTGAAACCAGGAGGTGGAGGTTGCAGTGAGC50.21691973969631237No Hit
GTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.21691973969631237No Hit
AGTTTATTGTATTCATTGGAATATGTGACGTAAGCAATAAAGGGAATGTTAGACGTGTAAAAAAAAAAAAA50.21691973969631237No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.21691973969631237No Hit
CTGTTTGTTTCAATTGGCCATTTAAGTTTAGTAGTAAAAGACTGGTTAATGATAACAATGCATCGTAAAAC50.21691973969631237No Hit
TTTATACACTTCTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.21691973969631237No Hit
CCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACAAGGTCAGGAGATCGAGACCATCCTGGC50.21691973969631237No Hit
CGTGCCGGTCCCCGATGTCACCGCCGCCGCCGCTGCTGCTGCTGCTGCTGCTGAGTCTGGCGCTGCTGGGC50.21691973969631237No Hit
GCCCTTCCTTCGTTTCCAGGCCTCTCCCCAGGCTTGCCATCAGCCTTCTTTACTTTTTGAGCCTCTGATTT50.21691973969631237No Hit
CTCTTGAGAAGGGGAGGATAAGTAGGCTGGGAAACTTCAAAGCCTTCCCAGTCCCAGCACCTGCCTTTCTC50.21691973969631237No Hit
GCTGTTCTGATAATAGGTTCTTAACATTGTATGTATATGTGTTTGCTTTGGGAGGAGGTGGCACTGTGTTA50.21691973969631237No Hit
GTATTTAATTAGTGCTAAGTGTGAACTGGACCCTGTTGCTAAGCCCCAGCAAGCAATCCTAGGTAGGGTTT50.21691973969631237No Hit
GGACACTTAGCAAATGTAAAAATAAAATCTAACTCTCATTTGACAAGCAGAGAAAGAAAAGTTAAATACCA50.21691973969631237No Hit
CATCCTTTTCCAGACACTGTAGATGCTATAATAAAAATAGCTGTTTGGTAACCATAGTTTCACTTGTTCAA40.1735357917570499No Hit
CGTACTGGGAAAGGGATTTTCAGCCCTCAGAATCGCTCCACCTTGCAGCTCTCCCCTTCTCTGTATTCCTA40.1735357917570499No Hit
GGTGGAGAGCTCTATGAAGAGTGGCTGGAACTTAGAAATGGTTGCCTCTGCTGTTCAGTGAAGTGAGGAAT40.1735357917570499No Hit
GGTGAGGGTGGTATTAGCATCTTTTTTATGTAGAAAAAATTGAGTTAATGGGGTGGACTGGGTTGGGAAGA40.1735357917570499No Hit
GGCATGAGAATTGCTTCAATCCGGGAGGTGGAGGTTGCAGTGAACCGGGATCACGCCATTGCACTCCCCCT40.1735357917570499No Hit
GAGCAGATGGGAGGACCCCGAGGCCAGACGCACACTGTCCGAACCCTCTGCTCAGCCGCCTGGGACCCACC40.1735357917570499No Hit
GTTTCTCCCACTGCCTCCTCTCCAGTGGTCTCCCAGGTGCCAGACCCAAAAGCTTTTCCTACAGTGATACC40.1735357917570499No Hit
GATTGTATGAGAATAAACTTGTTATTATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1735357917570499No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1735357917570499No Hit
CTTGTAAATACACCACCTCACTCCTGTTACTTACCTAAACAGATATAAATGGCTGGTTTTTAGAAAAAAAA40.1735357917570499No Hit
CTTTCATTTTTTGTAGTGACAGGAGTCTTGCTTTGTTGCCCAGGCTGATCTCAAACTCTTGGGCTCAAGCG40.1735357917570499No Hit
GTCTCTATGAATAAAGAAAAGCAAATAGAATCTTATCAGATAAGTGCTTTTATAAGTCTAAATATATTCTT40.1735357917570499No Hit
CCTACTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGAAATTATAACAAACTATCTCTCAGACC40.1735357917570499No Hit
CTCCCACTCCCACACTCACTCTTGCAGAGCCTGTGTCTTTAAGCAGCTGGCGTGTTACATCTCCATTTAAG30.1301518438177874No Hit
GTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGA30.1301518438177874No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
GGTACAGGGATTACAGCATTACCAGCCCAGTTCTGTGCCAGGTGCTGAAACTAGTTTTGTGGTAAATACAA30.1301518438177874No Hit
CCCAGACGCTGGTTCGAGAGTACTTTTCCTGGGGGGGGCCTTCCAGCATGTGGGCAAAGCCTTCAATCAGG30.1301518438177874No Hit
TTTGTAACCTAACCTATTGACCTGCATGTTTTTTCTTTACCCCAATTCATTACATGGAGGCTCAATCTTGA30.1301518438177874No Hit
AACTAGATATAACACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
AGCATGGTGGTACATGCCTGTAATCCCAGCTATTCAGGAGGCTGAGGCAGGAGAATCACTTGAACCTGGGA30.1301518438177874No Hit
ATTTTGAGTGGCAAAATAAAGAATAGCAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
ATCTAAAGAAGAGAAGGCTGTGGCCAAGTATCTTCGATTCAACTGTCCAACAAAGTCCACCAATATGATGG30.1301518438177874No Hit
AAGGTGCTGTTTGTAGACTTAACACCCAGTGAAAGCCCAGCCATCATGACAAATCCTTGAATGTTCTCTTA30.1301518438177874No Hit
GTTGCTGTGACTACAGGTGTGCACAACCACACTTGGCCACAGTTTTGCTTTATTTTTCTCAATTAAGAAGA30.1301518438177874No Hit
CCTTCTCTCTCTCTCTCTGTCTCTCTGGGTGTCTCTCTCTGTCTCTCTTTCTGGCTATCTCTCTGTCTGTC30.1301518438177874No Hit
GAATCTTTTAATAAGGTAGAAAGTTGCTTTATTTAAATTTGTACTGTAGAATGTTTGTGATACAGTATTTG30.1301518438177874No Hit
GTATTAAATGAAATCTTTTGAGTTTTTAGCCAAAAATTGGCATTTTTAAAATACGAAAATTTCCTTGGAAT30.1301518438177874No Hit
CAGTATGGTAAGTGCAGTAACGAAACATAAATATGCACAGAACTTTCCAGAAGAGGTGACCATTGGTCTTA30.1301518438177874No Hit
CAATAAAGGCTTGCATAGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
CCATTTGGAAGCTTGAGAGAAACCAAAATTAAAGAGAGAAAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
GGAACCTTCTTAATCTCTTCTGTGGGATTTTCAAAATGCTAAAGACTCACACTGCAGCAATCATCCCAGAT30.1301518438177874No Hit
AATGATGAGTGGGCGAGCCCCGGCTGGCAGGGGGCACATTGTCACTGGATCAGCGCCACAGCTGGGCAGCC30.1301518438177874No Hit
CATATTCTGATTTTATTAAAATAAAAAGTTGAACTGCACAGTCTCCTTTGTTGTTGTCAATTGTGGTTTAT30.1301518438177874No Hit
CCAGTCAAGTTTTTGGAAGGCGAGCTTATTCATGATCTTTTAACCATTTTTGTGAGTGCTAAATTGGCATC30.1301518438177874No Hit
GGAGGGAGGTTTGTGAGAGCGAGGCTGAGCCTACAGATGAACTCTTTCTGGCCTGCCTTCGTTAACTGTGT30.1301518438177874No Hit
TATTTACCCTATAGCACACCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
CACTAAAACAACTTTTTCCATTTTTAATAAAACGCCAAAAATATCACAACCCACCAAAAAAAAAAAAAAAA30.1301518438177874No Hit
GTAGAAAAAGCACAAGAGAGAAGCTTATCTATCAAACTGTCTGCATTAGTGCAATTAAGAACCGAAATACG30.1301518438177874No Hit
GCTACTTGGGAGGCTGAGGCAGAAGAATCACTTAAACCTGGGAGGCAGAGGTTGCAGTGAGCCGAGATCTG30.1301518438177874No Hit
CCTTAATATATGTTTTATGGAGATTATACATATTATAGATCTATATATGTAATAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
GTAACACACTTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
CTGCAAAGGGGCGTCGGACAAGTGCAGCTGCTGCGCCTGATGCTGGGACAGCCCCGCTCCCAGATGTAAAG30.1301518438177874No Hit
ATATACATTTTGTCCATAAACGTTATGCTGAATAGTTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
GAGGATCACCACGCTATTCTCCCCGTCATAGCAGATCTCGCTCTCGTACATAAGATGATTGGTGACACTTT30.1301518438177874No Hit
GGGCACGTCCGTGGTGCTAATCTGCCCGCAGGACGGCATGGAGGCCATCCCCAACCCCTTCATCCAGCAGC30.1301518438177874No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1301518438177874No Hit
GAACCTGAGGAGCCCCAACAACTTCCTGTCCTACTACCGCCTCACACGCTTCCTCTCCAGAGTGATCAAGT30.1301518438177874No Hit

[OK]Adapter Content

Adapter graph