FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19668
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5963.0303030303030303No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA1780.9050233882448646No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1630.8287573723815336No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT600.3050640634533252No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC570.28981086028065894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG560.2847264592231035No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC530.26947325605043726No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA520.26438885499288184No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT460.23388244864754934No Hit
ACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATT440.22371364653243847No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC390.19829164124466137No Hit
TGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTTT340.1728696359568843No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT340.1728696359568843No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC320.16270083384177345No Hit
GTCCAATTAGTTTCCTTTTTAATATGCTTAAAATAAGCAGGTGGATCTATTTCATGTTTTTGATCAAAAAC310.15761643278421802No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC310.15761643278421802No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT300.1525320317266626No Hit
CATGAAATAGATCCACCTGCTTATTTTAAGCATATTAAAAAGGAAACTAATTGGACCATTTTCTATTTGTC300.1525320317266626No Hit
GTATATATAATGGACAAATAGTCCTAATTTTTCAACATCTAGTCTCTAGATGTTAAAGAGGTTGCCAGTGT300.1525320317266626No Hit
ATAGATGACAAACGATGTAAATAATTTTGTAAGAGGCTTCAAAATGTTTATACGTGGAAACACACCTACAT250.1271100264388855No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA240.12202562538133008No Hit
GTACTAAATAGATTGTCTTCTAGAATTTCCTGTAGTCTGAATTATGTAGTATTGTTTCACATGTTCCAGTG240.12202562538133008No Hit
GGGAATCCACAACAGACTTTCTCTAGAAAACACTAATGATGGACAACTTCTTGGTGTCATCAATGAGTTGG240.12202562538133008No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC240.12202562538133008No Hit
TATCTATCCTGTGTGATAGTTTTGTTTGTTGTTGTTTTTTAAGGTAAAAGTCCTACTTCAGACCTTGAAGT230.11694122432377467No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.11694122432377467No Hit
AAATAGATCCACCTGCTTATTTTAAGCATATTAAAAAGGAAACTAATTGGACCATTTTCTATTTGTCTATT220.11185682326621924No Hit
AGTCAAGTGCCTAAGTTTTTAATATTGGACTTGGGTATTTATATATTGTGCATCAACTCTGTTGGATACGA210.10677242220866383No Hit
ATGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTT210.10677242220866383No Hit
TACATGAATTTAATACACGTGTTCTTAACAATTATGCTTGGATTGTTCATGAAAATTTCATAAGACATTAA210.10677242220866383No Hit
CCTCTAAGCTGCTTCCATCAGTCATTCATTCACAGCAGCCCCAACACAGAAAAACCTTCTGCCTGTACAGA200.1016880211511084No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1016880211511084No Hit
ACATGAAATAGATCCACCTGCTTATTTTAAGCATATTAAAAAGGAAACTAATTGGACCATTTTCTATTTGT200.1016880211511084No Hit
CAAATATAAGGCAAAGAGGAACTGCTGGCCACGAGTACGGGGTGTGGCCATGAATCCTGTGGAGCATCCTT200.1016880211511084No Hit
GAAAAGGGGAACACAAGGTGGTCGGAAAGGCCTTAAACTCCTCCTTGACATACAAAACCGGTTTTTACAAA200.1016880211511084No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1016880211511084No Hit
GTATATTTCTAGATATTGATTAGTGTAATCGATTATAAAGGATATTTATCAAATCCAGGGATTGCATTTTG200.1016880211511084No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1016880211511084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)