FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19083
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6913.621023948016559No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1400.7336372687732536No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA1330.696955405334591No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC1110.5816695488130798No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT860.4506628936749987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540.282974375098255No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA490.2567730440706388No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA470.2462925116595923No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC440.23057171304302257No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT430.22533144683749934No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC380.19913011580988313No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT380.19913011580988313No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.19913011580988313No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT370.1938898496043599No Hit
TATTTATATTTTGCCTGTTAAAAAGAAAGTGAGCAGTGTTAGCTTAGTTCTCTTTTGATGTAGGTTATTAT310.16244825237122046No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC280.14672745375465074No Hit
GTTTAATAGAAATCCTTAAAAAAGTAAGCCAACAAACAGAAAAGACAACAACAGTGAAATTCAACAGAAGA280.14672745375465074No Hit
AGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA270.1414871875491275No Hit
GCTCTGAAGAAGCAGCGTACCAAGAAAAATAAAGAAGAGGCTGCAGAATATGCTAAACTTTTGGCCAAGAG250.13100665513808102No Hit
GAATATGCACAGCACATGCAGTGCTAGCATCTAAACTAATACAATAAGCAATTACTAAAGCTACAGTGACT250.13100665513808102No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1257663889325578No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA240.1257663889325578No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1257663889325578No Hit
AGTTAGGCTTAATTTTTTTATCTAATGATCATCATGAAATGAATAAGAGGGCTTAAGAATTTGTCCATTTG220.11528585652151128No Hit
GGTTATTAAGGATAAACTGTTTAATCAAATTAACATCTCTTAAACAGTCTCTGAGAAGTACTTTACCTGAA220.11528585652151128No Hit
GAAGGATGGTCTTTTCAATAGCTGATTCTGGATCAACTGGTTGTCCCTGTGGAAAAAATGAACTTTAACCT220.11528585652151128No Hit
GTTCTAGATGACAAGGATTATTTCCTATTTAGAGATGGTGACATTCTTGGAAAGTACGTAGACTGAAATAA220.11528585652151128No Hit
CAACAGTGTGATTGACAGAAAATTGGATGGCATGTGCCTGGTCAGAAAATGTCATCTGTTTACAAAATAAC220.11528585652151128No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.11528585652151128No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCA210.11004559031598807No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC210.11004559031598807No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.11004559031598807No Hit
GCTTAAAGTTGTGTAATACTAAAATCACGAGAACACCTAAACAACAACCAAAAATCTATTGTGGTATGCAC200.10480532411046481No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAAT200.10480532411046481No Hit
AGATGGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCCTGGGAGACAGTGATAAT200.10480532411046481No Hit
GCGGCAAAAGTAGTAGAAAGCAAAAGGACAAGAACAATAACTTCTCAAGATAAACCTAGTGGTAATGATGT200.10480532411046481No Hit
CTTATGTATTTATGAATGGAAAAAGTTTATAATGCAAATTTCACTCATTAAAAAACTTAGGTACAAATTAC200.10480532411046481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)