FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3975
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103225.962264150943398No Hit
ATTGAAATAGCTACATGACCATAATGTGTTTAAAATGTGGTATGCTCTTGAGATATTTAAAGTTTTGGCAG230.5786163522012578No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT200.5031446540880503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160.4025157232704402No Hit
GCCATGGGCCCAGCCCCAAAGGGCAGTGAATGGCCTTCTCTGAAACCCTGCGTCAAGCAGTGGGAGAGGGC150.37735849056603776No Hit
CCTCTTACTTGCTTTCACATTTTGAGTGTCCAGAGGAAACAGAAAGGAATGAGTGTGTGACGTTGCTGCAC150.37735849056603776No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG140.3522012578616352No Hit
GCTTCAGTAGATTCACGGCAGTCTGGTGGAAGCTATTTGGATGCTGAGTGTTCACGACATAGAAGGGATAG130.3270440251572327No Hit
GTCTAGCCTTGTGTTATAGAATGAACTTGCATAATTATATACTCCTTAATACTAGATTGATCTAAGGGGGA130.3270440251572327No Hit
CATTCTAGTATATTCTTGTTTTATCTGCACTATAGATATGTATTGTTACATACCTTCAGCTTGGCAAGGCA130.3270440251572327No Hit
GTGTCACTGAGCTCATCAACGCCATGAAGCAAGTCAAGCACATTCCCGAAAGCAAGCTCACCAGCCTGGCC120.3018867924528302No Hit
CAATTTATGCAACAGATGTCCAGATATGATTCTATTTATGGAAAAAAGTTTATATGTGTTTTACAAATGGT120.3018867924528302No Hit
ATGTTATCTACATTGAACGGGTGCAGCGGGAAAAGGCTAATGGCACAACTGTCCACGTAGGCATTCACCCC120.3018867924528302No Hit
CCTTCACTGAACATGCCTAGTCCAACATTTTTTCCCAGTGAGTCACATCCTGGGATCCAGTGTATAAATCC120.3018867924528302No Hit
CGTCAGAGAAACGGCGTCATGCCCAGCCACTTCAGCCGAGGCTCCAAGAGTGTGGCCCGCCGGGTCCTCCA110.27672955974842767No Hit
GGTACAACTTATGGAAGGATTCATATGCAGAAGCAAGACCTAAGCAAACTACAAACCAGGAAAATGAAGGG100.25157232704402516No Hit
CTTTTAAATGGGTGAAAAATGGTAATATAACAATATATGATATATAAACCTTAAATGAAAAAAATGATCTA100.25157232704402516No Hit
GCTATTCACCTCAGTAGAAAAGGATCTAAGGGAAGATCACTGTAGTTTAGTTCTGTTGACCTGTGCACCTA90.22641509433962265No Hit
GCACTTTGGGAGGCCAGGGTGGGTGGATCATGAGGTCAAGAGATCCGGGCCATCTTGGCCAACATGGTGAA90.22641509433962265No Hit
CCTTTCCTGAAAGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.22641509433962265No Hit
CTATGAGGCCTTTATGAACATTATTAAATTGAGTGACAATATTGGAGAGCTAGAAGCAGTCAGAGATAAGG90.22641509433962265No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA80.2012578616352201No Hit
GGAGAGTGTCAGACTGATCTGGAAGTGAAACACAGTTTATGTACAGGGAAAAGGATTTTATTATCCTTAGG80.2012578616352201No Hit
GTTATCCATCTTGGTGCATCCTGACAAAAATCAAGATGATGCTGACAGAGCACAAAAGGCTTTTGAAGCTG80.2012578616352201No Hit
ACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAA80.2012578616352201No Hit
GATTTGCTCCTGGGTTCCCTAGGCCCTAGTGAGGGCAGAAGAGAAACCATCCTCTCCCTTCTTACACCGTG80.2012578616352201No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA80.2012578616352201No Hit
CCCTGCTTCAGGGACGTCCAGATCGGTGACATCGTCACAGTGGGCGAGTGCCGGCCTCTGAGCAAGACAGT80.2012578616352201No Hit
GGGTGCTCCTGCTCTGCACTCCAGAAACACCCTGACAGGCTCAGAGAGGAGATGGAAACTGACATACCCAA70.1761006289308176No Hit
GGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAAACAAACAACCCCATCAAAAA70.1761006289308176No Hit
CTTGGTAAATACGGCATCATCTGCATGGAGGATTTGATTCATGAGATCTATACTGTTGGAAAACGCTTCAA70.1761006289308176No Hit
TTTTTATACTTTAAACCCCGCTCCTCATGGCCTTGGCCCTTTCTGAAGCTGCTTCCTCTTATAAAATAGCT70.1761006289308176No Hit
GCCTGTTTGGTGGTCTCTTCACACAGATGCGCATGAAAATAAGGATGCCCACATTCATCATTTCTATTCAA60.1509433962264151No Hit
CCCCAGTGAGGACCTCGATGTCCAGCTGCTGTCAGGTCTGATAGTCCTCTGCTAAAACAACACGATTTACA60.1509433962264151No Hit
GAAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1509433962264151No Hit
GTGATTGGGTGGGATTTTTTCCCTTTTTATGTGGGATATAGTAGTTACTTGTGACAAGAATAATTTTGGAA60.1509433962264151No Hit
GGGGAGGGAGAGAACTAATGTTTCTCGTGCTTTGTGATCTGTTCAGTGTCACTCTGTACCCTCAACATATA60.1509433962264151No Hit
GGGCGTTCACTCAGACCATCTGTGCTCTGCCCTGAGTTCAGTTGAGAAAATCCTATTATCAAATTTGGATT60.1509433962264151No Hit
CCATTGAGCAGCTCCCAGACTACAACCGGATCCGTAGCGGCATGTTCTGGCTGCGCTTCTAGGCGGGAAGC60.1509433962264151No Hit
GTTTTCAAATTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1509433962264151No Hit
GGGTTAATGGCTAGTACTGTATTGGCTCTGTGAAAACATATTTGTGAAAAGAGTATGTAGTGGCTTCTTTT60.1509433962264151No Hit
CTTTGATAAAGTTTTTCATGCACAGGCAAAATGTATTCACTAGATTTCTACGTAGTGATCTGCTTTTACTT60.1509433962264151No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAAC60.1509433962264151No Hit
GGAACCAGGGTCCGGTTGCGGCTATTAGATCTTGAACTTACGTCTAGGTTCCTGGGAGCAACAACAGATAC60.1509433962264151No Hit
GCACTTTGGAAGGCTGAGGTGGGCGGATCACCTGAGGTCAAGAGTTCGAGACTAGCCTGACCAACATGGAA60.1509433962264151No Hit
GATTTACTGAAGCAGGAGAGCGGGTACGAGTCTCCACACGATCAGGGAGAATTATCCCTAAACCCGAATTT50.12578616352201258No Hit
TGAGGGGACAGTGCAGCTACAGGATATGCCATCATGGCAGAAAGTTCTATTGGTTGGACAGTGTTGGTCTA50.12578616352201258No Hit
GGTCCTCCTGCCTCAGCTTCCAAAGGCACAGGCCAAGTTGTAGCTTTGTCCCTTGCCATCATGCCCAACAA50.12578616352201258No Hit
CAATAAAGAGAGGCCCAGAGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12578616352201258No Hit
CAGATTTTTATGTTTTCTTTCTCACCCAGGCAATCTTGCCTTCTGGTTTCTTCCAGCCACATTGAGACTTT50.12578616352201258No Hit
CAATAAAGCTAACAATAAACCTTATTTAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12578616352201258No Hit
AGCGAACACAGTGGGCACAGTATAGAGAATCCGCAGAGAGGGAAATAGCTGATTTAAGAAGAAGGCTGTCT50.12578616352201258No Hit
GGCTCAGTGGACTGATATCCCAAAAGGCTGATCATTGAACAAAGACTGAGTGGGGTATTTATAAGCAAAAT50.12578616352201258No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12578616352201258No Hit
ACTATATTGAATGCATGTGTTTTTCAGATTCCAGTTTAATTAAAATCAGTGAGACAGAAAAAAAAAAAAAA50.12578616352201258No Hit
CTTGATGTCAGATTTTATAGTCTTTTTAAATAAATCCATTTAATTAAAAAAAAAAAAAAAAAAAAAAAAAA50.12578616352201258No Hit
CCCTCCCCAGCCCATTCCTGCTGTCAAACAAACAAAACCTTGCAAAGCAAAAAAAAAAAAAAAAAAAAAAA50.12578616352201258No Hit
GGAGTTCAAGACCAGCCTGACCAACACGGTGAAACCCCGTATCTACTAAAAATACAATATTAGCCACGTGT50.12578616352201258No Hit
CTCCTGCAACCTGCTGGGCATGTGGGCCTGGCTGTGTTCAATTGCAAGAACAATTTTTATGAAATGGATTA50.12578616352201258No Hit
CCTAGACCCACAGCCCCTCGGGAGCACCCCACCTCTGTGTGTGATGTAGCTTTCTCTCCCTCAGCCTGCAA50.12578616352201258No Hit
GATAGGTACCTAAAATGTAACATGAAAATACTAGCTTATTTTCTGAAATGTACTATCTTAATGCTTAAATT50.12578616352201258No Hit
CCTTTGGAGGGTGTCTTCTGGGTAGAGGGATGGGAAGGAAGGGACCCTTACCCCCGGCTCTTCTCCTGACC50.12578616352201258No Hit
TTCTAATACATATTAAAAGACATAACTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12578616352201258No Hit
TCAATATCCCTCTTGTAAGTCATCTACTTAATAAATATTACAGTGAATTACAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
CACACACACAGCCGAGGAGGGGATTAACCGAGTGAATGTGTTTCAGGAGCTCCGTCACCAGGAAGCAGAGA40.10062893081761005No Hit
CCCCTGAGAGCGAGGAGACGCCCCTGACGGCCGCGCAGCTCTTCAGCGAAGCCGTGGCGCCTGCCATCGAA40.10062893081761005No Hit
GACTTCAGTCATCCCAGCAATCGCTTGGTTCAGTTTCATTCAGCTCTCTATGGACCAGTAATCTGATAAAT40.10062893081761005No Hit
CCTGTGTTTGCATATTTTTTTGGAGTGCAGAATATTGTAATTAATGTCATAAGTGATTTGGAGCTTTGGTA40.10062893081761005No Hit
ATCCCAGACTGCCCTCGTCCCAGCTCTCTGTCCAACTGTTGATTATGTGATTTTTCTGATACGTCCATTCT40.10062893081761005No Hit
CAGCGATCTGAGCATAAAACTCATTAAACCTGCCCCTATCACTAGTGCATGCTGTGGCCAGACAGATGACA40.10062893081761005No Hit
CCTGTAATCCCAGCACTTTGTGAGGCCAAGGCAGGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTG40.10062893081761005No Hit
ATTAAAAAGAGCCTTACTTTCTTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
GTCTGTTTTTAATACCATATACTTTATATTTCTATACATTTATATTTCTAATAATACAGTTATCACTGATA40.10062893081761005No Hit
TCTCTCCACCATGCACCTTGTGACCCCCTCCTCTGCTGAGGATAACCACCTTTAACTGTAACTTTCCACGC40.10062893081761005No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
AAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
CAACAGGATTCTAGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
GAGTAATGTTTGCCTATAAAATTGTCAGCGTATTTTTACACTATTGGCTCAAGAATGTTATAATGCTAAGG40.10062893081761005No Hit
GCAGTGCCCAGTGATGGCATTACTCTGCACTATAGCCATTTGCCCCAACTTAAGTTTAGAAATTACAAGTT40.10062893081761005No Hit
CCCTTCTTGTCTTCCTTCCCAGCAGCGCCCGCAGCGGGCCATTTACACGTCGAGGCTGGCACCTGGCGCGC40.10062893081761005No Hit
GAGCAAGAAGGAGCTGAAGGAGCTGATCCAGAAGGAGCTCACCATTGGCTCGAAGCTGCAGGATGCTGAAA40.10062893081761005No Hit
AAATAATACATTGTTCTAAGTCTTTTTCATTAAAAATTTAAAACTTTTCCCATAAACTCTATACTTCTAAA40.10062893081761005No Hit
AAACTGGCCTTTCTGCCAATAAAGTTGCATGGTAAATATTTCATTACAGAATTTATGTTAGAGCTTTCATG40.10062893081761005No Hit
GCATCCAACTGTGATGAGCCTATGTATGTCAAGTTGGTGGAGGCCCTTTGTGCTGAACACCAAATCAACCT40.10062893081761005No Hit
ACAATGGGTGCTGAGAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
GCGTCAGATGTGTATAAGAGACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
GTTTATTGAGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
GCCTCCAAGGAGACTCATGTAATGGATTACCGGGCCTTGGTGCATGAGCGAGATGAGGCAGCCTATGGGGA40.10062893081761005No Hit
CTAGGACAAACACCAAAATATGCAACTTTTTTTTGGTGGGAAGAGAGATTGTCCTGTGATTTCTACCCATT40.10062893081761005No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
ACAGCTCAGCTTCAGGCTTCTTATGTTTCTGAAAACAGCTTGGATATTTTAATGCACGTTGCATTAAACCT40.10062893081761005No Hit
CCATATTGGCCAGGCTGTTCTCAACTCCTGACCTCGTGATCCGCCCACCTCAGCCTCCTAAAGTGCTGGGA40.10062893081761005No Hit
GAACAGGAGACCCACCTGAGGCTCAGCCCTAGCCCTCAGCCCACCTGGGGAGTTTACTACCTGGGGACCCC40.10062893081761005No Hit
GAGAGTTTGGCCGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCGAGTGAACACCTCGAGTGGTACGCC40.10062893081761005No Hit
CCCCCAAGAAGAACTGAATAAAGATTGCTGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10062893081761005No Hit
GCTTTGGAATGCTCAAGGCCAAGGCCCAGGCTTTGGTGCAGTACCTGGAGGAGCCCCTCACCCAAGTGGCG40.10062893081761005No Hit
ACTTCTGATTTATTGCAATGTTCAGTCCTAGTTTTTACCTTTATTCTTAAAGCATTCAGTTTTGCTTTCAA40.10062893081761005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)