FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10827
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6045.578645977648471No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1531.4131338320864506No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA330.30479357162648935No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG220.20319571441765957No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC190.17548720790616054No Hit
GTTAATAAACAGTACCTGCTCTCAAATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15701487023182784No Hit
GAAGAAGGGCATCAAAAATGTCATGCTGAACACACTCCAGAAGAGGAAATTGATCATACAGGAGCCAAAAC170.15701487023182784No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT170.15701487023182784No Hit
GTAGGGGCCGGTGGACCTGCTCCAGCAGCTGGTGCTGCACCAGCAGGAGGTCCTGCCCCCTCCACTGCTGC160.14777870139466148No Hit
GTCCCACCTGGCTCCCCCTGCTGGTGTCTAGTGTTTTTTTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAA150.13854253255749516No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCT150.13854253255749516No Hit
ACATCATTGTTGGTGGCTGAATACATTTTGGAAGAGAGTTTTTCATCTTAGAGATTGGTGAACAAGTGTGA150.13854253255749516No Hit
CCAAGAGTGTGGCCCGCCGGGTCCTCCAAGCCCTGGAGGGGCTGAAAATGGTGGAAAAGGACCAAGATGGC140.1293063637203288No Hit
AATCTTATGTATTGGTGTAAGAGTTGATGAATGACTTTAGCTGTGTGAATATATAATAGTCAAACTGCAAA140.1293063637203288No Hit
TTCTATTTTAAGTATATTATTTATTGTTGGCAGTTCCTCAGGGATTTCCCTTTTCTCTGTATTGGTCAGTG140.1293063637203288No Hit
ATTTAAAAATAACTTACATTTTTAGTGTGCTTTATGGTCAATGACTGTAAAGTTGAAACTCAAATGAGTCA140.1293063637203288No Hit
TGCATTAACATCAGGCACGATAAAAGCCATGTTGAGTGGCCCAGGTCAGTTTGCTGAGAACGAAACCAATG140.1293063637203288No Hit
GAGTAATGATGTGTTTGACATATAGTGAGTTAGTCACTACTTGACTTCCCTCTATACAGCAAGTCAGTCTC140.1293063637203288No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTC140.1293063637203288No Hit
GCTATCAACTGTATGTTGATCACAGTTTATAAGAAAGAACAAATCAAGATTGGCAATCCTTGCCGATCTTT130.12007019488316245No Hit
TTACAGACATGAGCCACTGTGCCTGTTCTGAAGGAAATTCTTAGCATATAAATAAATAAAGTGTACTGGAT130.12007019488316245No Hit
CAAAAAGAGTATCTTTGTTTTCACTCATGTACTTGTACCTGTCTGGATTATTCATTATTACATGAAGTATC130.12007019488316245No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT130.12007019488316245No Hit
CAATACCTGGACCCCGAGGAGCCTGCTTGCTGGAAAGGCTTTCCTGTCTGATGTGCAGGAGGCAGAATGCC130.12007019488316245No Hit
CCATAGCACATTGAGCTGGGAAAATTCCCTTTTCCCTTCAAACACATAAATCCTGTTGACCATTAGGCTAT130.12007019488316245No Hit
GCGTAACTCCAGACATGATGGAGGAGATGTATAAGAAAGCTCATGCTGCTATACGAGAGAATCCAGTCTAT120.11083402604599613No Hit
GTTTACCCATCTGTAAAATGGGTAAGCATAGCCACTGGTGGGATATTTTGGGATGTCAAGGGGTGGAGGCA120.11083402604599613No Hit
CTACAAAGGTCAGCAAATTGGCAAGGTAGTCCAGGTGTACAGAAAGAAATATGTCATCTACATCGAGCGGG120.11083402604599613No Hit
GTATGAGAACAAGTAGTTCCTTGGAGGCCCCCATCCAGGCCAGAAGGACCAGGTCCACCCAGCAGCTGTTT120.11083402604599613No Hit
ACTTAAAACTATAAACCTCATAGAAGAAAACAGGAAAAATTTTTCGTGGTTTTTGGGTTAGGTAATGGTTT120.11083402604599613No Hit
GTGTTAGCTCAGTGAGCTAGGAAAGCACCAATCGATATTTGTTTCCTTTAGGGATACTTTGTTCTCACCAC120.11083402604599613No Hit
AGCCTGTGTGAGACTCCGTTTCTCTATCTCTAAAATGGAGTCCGCCAGGCGCAGTAGCTCATGCTTGTAGT120.11083402604599613No Hit
ATCCAACTGTGATGAGCCTATGTATGTCAAGTTGGTGGAGGCCCTTTGTGCTGAACACCAAATCAACCTAA120.11083402604599613No Hit
GACGATATGGATGAAAAGACAACCCTACAGCTGCCAAATTCCTTTGATTAAATGTGTGAGCTGGTTGATAG120.11083402604599613No Hit
TTACAAGGTAATACAATGAATAAGTTCATACTATGAAATGACACAACTTTGATGACATGGAAAAATTCCTA120.11083402604599613No Hit
CCACAAAACTCAACAATAAAAACAAACAGTCCAATTAGAAAATGGGCAAAAGACATGAATAGATGTTTCAC120.11083402604599613No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11083402604599613No Hit
TTGGGAGGCCGAGGCAGGCAGATCATGATGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTGAAACCCT120.11083402604599613No Hit
TCATAATACAGATTACTGGCTTTATACAAGAAACCGCAGAGGGCTCTCATCCTCCTGCAAAATGAGGGTAA120.11083402604599613No Hit
CCTATGGTGGTTCCATGTGTGCTAAATGTGTTCGTGACAGGATCAAGCGTGCTTTCCTTATCGAGGAGCAG110.10159785720882979No Hit
TCATTAAACATTGTACATCATTTCAAAACTGTTATCTCATAATCATGTTGCTTGTTTTGAATTATTCTTTT110.10159785720882979No Hit
CATTAATAATATGAGAAATGATACCTGGAGAGAGAGATTATGAGCAAATGTATTGCTTCTTTTAGAGGAGG110.10159785720882979No Hit
TATTTCTTCTTAAGAAGTCTTTCAAGTTCTTCACCAAAAATGTTGACACGTCTCCTGGGAAGCTTATTGTA110.10159785720882979No Hit
GGCTGCCAGTTTCTGCTGATACACAGGTAGGATGGGACCCTTCATGAATATCTGACTTTAATAAGTTGGTA110.10159785720882979No Hit
GGTGGAGAGCTCTATGAAGAGTGGCTGGAACTTAGAAACGGTTGCCTCTGCTGTTCAGTGAAGTGAGGAAT110.10159785720882979No Hit
CTGTCTCCCCAGAGAATCTTGTCAAGGCTTGGCTTTAAGCTTTGTTGGGAAAATCAAAGACTCCAAGTTTG110.10159785720882979No Hit
CTTCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACCAACCTCCACAGGCTGGGTG110.10159785720882979No Hit
GGCTAGTCTCGAACTCCTGACGTCGTGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTG110.10159785720882979No Hit
CACTGGCTATCATTGATCCAGGTGACTCTGACATCATTAGAAGCATGCCAGAACAGACTGGTGAAAAGTAA110.10159785720882979No Hit
GGATATATATTTGTGAAAAATAATTTTTACTTATATTTTTCAGAGGATTTGACACGATAAGCCTCATCTGA110.10159785720882979No Hit
TTATTTGCACAGACTTAAATACAGTTAAATGTGTTATTTGCTTTTAAAATTATAAAAAGCAAAGAGAAGAC110.10159785720882979No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.10159785720882979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)