FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564
Sequences flagged as poor quality0
Sequence length71
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56021.8408736349454No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG210.8190327613104524No Hit
CATCGAACAGTACGCACTGAGGGAAGAGCACATTGATAAGGTGTTCAAACATAAGGAACTGCAACAGCAGC170.6630265210608425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160.62402496099844No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA150.5850234009360374No Hit
GCGCAAGATTGATCAGAAAGCTGTGGACTCACAAATTTTACCAAAAATCAAAGCTATTCCTCAGCTCCAGG150.5850234009360374No Hit
ACTGTGGACTAGTAGAAAGTTCATATTCTACCTCTAAAAACAGGTAGTGGTAACCTGACTCTTCTAATCTT140.5460218408736349No Hit
CTTGGTAAATACGGCATCATCTGCATGGAGGATTTGATTCATGAGATCTATACTGTTGGAAAACGCTTCAA130.5070202808112325No Hit
GGAGACTGGCTTTCACAGAGGTTTGGGTTGGGTTCAGTTTTCTTCAGAAGAAGGACTTCGGAATGCACTAC120.46801872074883No Hit
CTTTTTAAATTTTTTTGTACTATGTTTGGTTTTATTTTCCTTCTGTTAATCTTTTGTATTCACTTATGCTC120.46801872074883No Hit
GGATACAAGATTAACATACACAAATCAGTAGCTTTTCTATACACCAACAGCGACCAAGTGGAGAATCAAAT110.4290171606864275No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT110.4290171606864275No Hit
GTGTTTAACAATTGGGGCTGTTAAGTCTGACCATACATCACTGTGATAGAATGTGGGCTTTTTCAAGGGTG90.3510140405616225No Hit
TCTTAGAGCAGTAGCTTGCAGTTTCAGTTGAGTTCTACTTAGAAATTCTTTTTAGCTAGTGGGCATGTGAA90.3510140405616225No Hit
GCACCACCGTAAACAAAGCGGAACTTTCATATGGACGTGCAAATCCTGTGTGTCCTTGGAATCCTAGTGTT90.3510140405616225No Hit
GGGGTTGGCTCCATGACCAAGATCTATGGGGGACGTCAGAGAAACGGCGTCATGCCCAGCCACTTCAGCCG90.3510140405616225No Hit
GGCCAACATGGCAAAACCCTGTCTCTACTAAAAAAACAAAAATTAGCTAGGCGTGGTGGCATGTGCCTGGA90.3510140405616225No Hit
CATTGTGCCATGAGGTAAATGTAATGTTTCAGGCATTCTGCTTGCAAAAAAATCTATCATGTGCTTTTCTA90.3510140405616225No Hit
GTCATAGGCTTCTTTCAGTCTTTATGTCACCTCAGGAGACTTATTTGAGAGGAAGCCTTCTGTACTTGAAG90.3510140405616225No Hit
AGTTCTACAAAATATTTATCAAATGAATGTTAATTTTTTTTTAATTCCCTGCTAAAGACGTTTTCATTAGT90.3510140405616225No Hit
GTTGATCCGCAGGGAGAAGAGAAGTGTAAGGCTGCCAGGAAACAAGCTGTGAGGCTTGCGCAGAATATTCT80.31201248049922No Hit
GGGCCCTCCCTGCACTCCCCTCTTGCTGCGTGTTGATTTGGAGGCACTGCAGTCCAGGCCGAGTCCTCAGT80.31201248049922No Hit
CACTAAACCAAGGTCATGAGCATTCGTGCTAAGATAACAGACTCCAGCTCCTGGTCCACCCGGCATGTCAG80.31201248049922No Hit
ATTTTTATCATACAGTAGATTCCATCCATTCACTATACTTTTCTAACTGAGTTGTCCTACATGCAAGTACA70.27301092043681746No Hit
CCTCCCATCTGCCTTGAGATTCCCTCGTCTTCACTTAAAAGCCAGAGTGCAAGTCATGATTTGCGGGAGGG70.27301092043681746No Hit
GGCTGCAGAGAGACTAAGGGCACTTCCTGAGGTTCATTACCACCTGGGGCAGAAGGACAGGGAGACAGCAA70.27301092043681746No Hit
GTTAAAGTCAAAGGGGCATATATAGAGTAAGGCTTTTGTGTATTTAATCCTAAAGGTGGCTGTAATCATGA70.27301092043681746No Hit
CATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGATGGG70.27301092043681746No Hit
ATATATACATATCTAGGGCTAGTACTTAGTTTCACACCCGGGAGCTGGGAGAAAAAACCTGTACAGTTGTC70.27301092043681746No Hit
GCTGGATGTGGTGGTGCACACTTGTGGTCCCAGTTACTCAGGAGGCTTAGGCAGGAGGATGGCTTGAACCT70.27301092043681746No Hit
GTACATAGACCAGTGACAATAAAAAGCAACCACAAAAGCAAGTCTGTAAAATAACTAGCTAACATAATGAC70.27301092043681746No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT70.27301092043681746No Hit
GTATGACTGATGATCCTCCAACAACAAAACCACTTACTGCTCGTAAATTCATTTGGACGAACCATAAATTC70.27301092043681746No Hit
GTCAGGAACATTTGACTGCTTCCATGTTGGCATCTGCCCCTCCTCAAGAGCAAAAGCAAATGTTGGGTGAA70.27301092043681746No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA60.234009360374415No Hit
CGTTCTGCTGTCAATCTGAAAATGCCAGTGCTGTGCCTTGGAAAGAATGTTTGGCTTTAATTTAAGGGTTT60.234009360374415No Hit
TGCTTCTCACCGTGCAGAGCTGCACTTGGCCTCAGCTGGCTGTATGGAAATGCACCCTCCCTCCTGCCGCT60.234009360374415No Hit
GGAAAAGCATTTGATAAAATTCAACATCCCTTCATGATAAAAAAAAAAAACTTAAACTGAGTATAGAAGGA60.234009360374415No Hit
GGTCCAATTTATATATAGCTGAGTCCACCTCAGGACGAGGCCAGTGCCTGAAACGCATCCGCTACCATGGC60.234009360374415No Hit
GGGCGCGGTGGCTCATGCTTATAATCCCAGCACTGTGGGAGGCCGAGGCGGTCAGATCACGAGGTCAGGAA60.234009360374415No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACAC60.234009360374415No Hit
CAATCAATCTTCTATTGATGAACATGATCACAAATATGACTTTTTCTTATAGCTGACTTTGCTATTAAATG60.234009360374415No Hit
GGGTCACAGACTGGGATGAGCAACGGGCTGAAGGCACGTTTCCCGGGAAGATCTGAACTGGCTGCATCTCC60.234009360374415No Hit
ATCACACCCCATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.19500780031201248No Hit
GTGAAGAGGAAGAGGTCGATGAAACAGGTGTAGAAGTTAAGGACATTGAATTGGTCATGTCACAAGCAAAT50.19500780031201248No Hit
GGCTGGGAGCCATGAACATGGAGAATATCCTTGGATGCTGCATTCATAGGAGAATTGAATAATTTCTATCA50.19500780031201248No Hit
GGACCAGGGGCTGCTGGGGATGTGTGAGGGGGAAAAGCGCAAGCTGGTGATCCCATCCGAGCTAGGGTATG50.19500780031201248No Hit
GATATGCTCATGTGGTGTTGAGGAAAGCAGACATTGACCTCACCAAGAGGGCGGGAGAACTCACTGAGGAT50.19500780031201248No Hit
ACATGGAGCCAAAGACAAAGATGACTGAAAGAACTTTAGCTAAAATCTTCCAGTTACATTGTCTTACTCTC50.19500780031201248No Hit
CTACTCTGAAGCTAGACATGTGCTTTGAAATGATTATCATCCTAATATCATGGGGGAAAAAATACCAAATT50.19500780031201248No Hit
ATTATGAACTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.19500780031201248No Hit
GTAATCCTAGCACTTTGGGAGGCTGAGGCGGGCGGATCACCTGAGGTCAGGAGTTCAAGACAAGCCTGGCC50.19500780031201248No Hit
CGACGCTCTTCATTTCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.19500780031201248No Hit
TTGTTACAGCAGTGCAAAATAAAATCCTATGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.19500780031201248No Hit
GTTACATTTTTGCTTTTAACCAATTAAAGAAGCCAATGGCATTTTAGTTTTATATTGTGTTTTCCACTAGT50.19500780031201248No Hit
GTCTAGAGATATATATACTTGTGGTTTTCAAATTGGACTTTCAAAATTAAATCTGTCCCTGAGAGTGTCTC50.19500780031201248No Hit
ACACTTGGTGTACTGGGTTAATGGCTAGTACTGTATTGGCTCTGTGAAAACATATTTGTGAAAAGAGTATG50.19500780031201248No Hit
CTTGGTATATAAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.19500780031201248No Hit
CTAGAATTATGTTACTGTGTCTTATTGGTTCAATGATGATTTCTCTGGCATATTAGACTTTTATTTTTTCC50.19500780031201248No Hit
AGTGTGTTTTGAGATGAAGGTTACAGAGAAGATCCCAGTAAGGCATTTATATACAAAAGATATTGACATAC50.19500780031201248No Hit
CCTCCTAGAGGACCCAGCCGCCATCAGCCCAACGGGAAGCAACCTCCCAACCCAGAGGAGGCAGAATAAGA50.19500780031201248No Hit
CATTAACTGTCAGGACAATGAAGGCCAAACAGCTCTACATTATGCCTCTGCCTGTGAGTTTCTGGATATTG50.19500780031201248No Hit
CAGCTAGAAACAGTTGACTGACTAAATGGAAACTAGTCTATTTGACAAAGTCTTTCTGTGTTGGTGTCTAC40.15600624024961No Hit
GGACCTCACTCAGAAAGACTAAAGGAAATGTAATTTATGTACAAAATGTATATTCGGATATGTATCGATGC40.15600624024961No Hit
GATCACGCCACTGCACTACAACCTGGGTGACAAGAGTGAAACTCCATCTCAAAAATAAAAAATTTAAAGAA40.15600624024961No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC40.15600624024961No Hit
GTGTAAAATATTTTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15600624024961No Hit
GATAATACAGGAAAAACGGGGTTACTAGTAGCCGCCCATAGCCTGCAACCTTTGCACTCCACTGTGCAATG40.15600624024961No Hit
ATCCAAAAGAGCAATAAAAAGTTTTCAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15600624024961No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15600624024961No Hit
GTCCAATAAACCTCTTCCTTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15600624024961No Hit
CATCTTGGCCCTCCTCAGCTCCCTGCCTGTTTCCCGTAAGGCTGTACATAGTCCTTTTATCTCCTTGTGGC40.15600624024961No Hit
GACATGTGAGTAAGATAAATGTATACAATTGTGGATTTAATTGTGAAATGTTCTTTCACTTGTAAGTTTCA40.15600624024961No Hit
AGGTTTTAATGATGATGTTCTACTTCAGATAGTTCACTTTCTACTGAATAGACCAAAAGAAGAAAAATCAC40.15600624024961No Hit
GAACTCTCTGTGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTCACAGGACTCAACATACT40.15600624024961No Hit
GCTACTTGGGAGGCTAAGGTGGGTGGATCACTTGAGCCTGAGAGGTTGAGGCTGTGGTGAGCCATGGTCAC40.15600624024961No Hit
ATTTATGATGTTACGGTGGTTTATGAAGGGAAAGACGATGGAGGGCAGCGAAGAGAGTCACCGACCATGAC40.15600624024961No Hit
TTATTATATGTTCTACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15600624024961No Hit
ACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAGGG40.15600624024961No Hit
GCACTCAAGGGCAAAAAGGGAAAGGAAGAGAAGTCAAAAGGGAAGGCTAAGCCTCAAAATAAATTCGCTGC40.15600624024961No Hit
GCACTTTGGGAGGCCAAGGCGGACGGATTACCTGAGATCAGGAGTTCGAGACCATCCTGGCCAGCATGGCG40.15600624024961No Hit
ATTTTGAGGAGATGAGGAAGGCTGGTATCTTTCAGAGTGTAAAGTAATCTTGGAATATAAAGAATTTCTTC40.15600624024961No Hit
ATCCTACTCTCTTATTAAAAAGATTTTTGCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15600624024961No Hit
GGACTCATCATCAATAAACACTGTTACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15600624024961No Hit
GTTGCTAATATGCAGAAGGGATGGGTGATACTTCTTGCTTCTCATGATGCATGTTTCTGTATGTTAATGAC40.15600624024961No Hit
GTTGAAATTAAGAACACTTGTACCAGTGTTTATACAAAAGATAGAGCTGCTAAATGTAAAATTCCAGCCCT40.15600624024961No Hit
CTCTTCTCCTGACCTGCCAATAAAAATTTATGGTCCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15600624024961No Hit
AAATTCTACTTCCTGTCCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15600624024961No Hit
GGCACAGACCCGGGCAGAGGGACAGCGCCAGGCCCAGGAGTATGAGGCCCTGCTGAACATCAAGGTCAAGC40.15600624024961No Hit
GGCGCAGTCTCAGCTCACTGCAACCTCCGCCTCCCGGGTTCAAGAGAGTCTCCTGCTTTAACCTCTGGAGC40.15600624024961No Hit
ATCCACCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGGTGTCATCAAAGCAGTG30.1170046801872075No Hit
CAACAACCTTTGCAGTTGTTGGTGGAGTATTCTACAGACGTCTGCCAAGTCTAGGTGGGTTAGAGCCTGTT30.1170046801872075No Hit
GAGACGCCTACATGACCTGCCTGCCGAGAGGAACCACCCTCTTCAGGGCCTCATCTCTGCTGAGAGCTGAA30.1170046801872075No Hit
GAAGTGGAGCCAGAGCCAACTGAGGACAAGGATTTGGAAGCTGATGAAGAGGACACTAGGAAAAAAGATGC30.1170046801872075No Hit
TTGTTTCACACATACATCAAACAGGCCAAAAAAAATAAACAGCAACTTCATAGACAAAAAAAAAAAAAAAA30.1170046801872075No Hit
ATATAAGGGAGAAATACATGGTTTTTGTGGAAATCATTATATGTTTGCTTTAGATTTTCTTCTGTTTTCAC30.1170046801872075No Hit
GTTGAAAATACAGCTACAATCTTTTTCTGCTCTCCTCCCAACTCATCTTGCCCCTCCCTACCTCTGAACAG30.1170046801872075No Hit
GTATGATATTCAATAAAATTTTCTAAATAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
CTACATTCCTGAAACAATTTTTAGGTTTTTTTTGTACTAGAAAATGCAGGCAGTGTTTTCACAAAAGTAAA30.1170046801872075No Hit
ACACGACGCGGGACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
TCCCTACACGACGCGGGTTTTCATAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
GCATTTATTTTTAGTACATTTTATTTTTTCATAAAATTGCTAATGCCAAAGCTTTGTATTAAAAGAAATAA30.1170046801872075No Hit
GGAGAATGGTGTGAACCCGGGAGACGGAGTTTACAGTGAGCCAAGATCGCAGCACTGCACTTCAGCCTGGG30.1170046801872075No Hit
GCTCGCCATCCTGCCCGAGGCGGCTCGGGCCCGGCGGATCCGCCGCCGCACGGATGTGCGCATCACTGGCT30.1170046801872075No Hit
CCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
GAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGATGGGAGCGCCCG30.1170046801872075No Hit
TTACATCTGCTGATGCAAATACATATAACCTTATTGAGTTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
GCTCTTCCTACTGTCTTATGAAGAAGGTTCCTTGCTTCCCCTTCACCTTCTGCCATGATTGTAAGTTTCCT30.1170046801872075No Hit
GTTCGAGCGTGCCAGAGGCCGACGGGCCAGCCGAGGCTACAAAAACTAACCCTGGATCCTACTCTCTTATT30.1170046801872075No Hit
ATGTTTGTAGTGCCAGCTACTCAGGAGGCTGAAGCTGAAGCATAGCTTGAACCCGGGAGGCGGAAGTTGCA30.1170046801872075No Hit
GCTTGATGGTGACTCTGCAGGAGACTCTGAAGTGACTGCTGGGAAACCCTTTGGGAGACCTGACCTGGGGC30.1170046801872075No Hit
GCTTACAGCATAGTTGGCTTAAATGAAAATAAAATGATATGCTTATACATTTTAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
TATTTATCTTTCTGGGTATTTTTATAGCCCTTAATAAAAAATATTAAAATAGAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
TGGCTGAGGGTTCTGCTGTCCTGTGCCACCCCATTAAAGTGCAGTTCCCTCCGGGCCAAAAAAAAAAAAAA30.1170046801872075No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
CCTCTACTCTTTGAGACATCACTGGCCTATAATAAATGGGTTAATTTATGTAAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
GCACTTGTTGCTTTGCTGAAAACTATGGTTAACCCTATATAGGTGTGGGAAATTTTTGTCACTGCATAATA30.1170046801872075No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit
CCAAAAAAGCAAGAAGATTCTGTCACTGAGTTAACAGTGGAAGATTCTGGTGAAAGCTTGGAAGACCTCAT30.1170046801872075No Hit
CTGTATACCTATAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1170046801872075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)