FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4162
Sequences flagged as poor quality0
Sequence length71
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1954.6852474771744355No Hit
GAACAGATAGCCACCAGTTACGGCCATTGTGTGTAACTCCTAAGTACTGTAGTCTCTGGGTTACACACAAT100.2402691013935608No Hit
TCCCTACACGACGCGGGCACTCTTTCCCTACACAACGGCATCCGAGATCGGAAGAGCGTCGTGTAGGGAAA90.2162421912542047Illumina Single End PCR Primer 1 (100% over 27bp)
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.19221528111484865No Hit
TTCCCTACACGACGCGGGCACTCTTTCCCTACACAACGGCATCCGAGATCGGAAGAGCGTCGTGTAGGGAA80.19221528111484865Illumina Single End PCR Primer 1 (100% over 26bp)
AAGCTACGTTGGAGAACTCTGGACTTAACTTACAGGAAGATAAAGAGGATGAGAGCATCAGAAAAACAGAA70.16818837097549255No Hit
AAGCTATTGATTCTGAAGTAGTGGGTTTTGATTGAGTTGACTGTTTTTAAAAAACTGTTTGGATTTTAATT70.16818837097549255No Hit
AGTATAGTTAGTGTTGTGGTAATGCCTGTTTTCATCTGTAAATAGTTAAGTATGTACACGAGGCACTACTT70.16818837097549255No Hit
CATCTAAATAAGACAGACACTTCCATTACCAAAGTTTACTGTTCTGTTTAGTATGTTCAATGAAAAAATCT70.16818837097549255No Hit
GGTAAGAAACCTAGGACCAAAGCACCCAAGATTCAGCGTCTTGTTACTCCACGTGTCCTGCAGCACAAACG70.16818837097549255No Hit
CCTGTGTGCTATCCATGCCAAACGTGTAACAATTATGCCAAAAGACATCCAGCTAGCACGCCGCATACGTG70.16818837097549255No Hit
TCTCAGGGTTATGCTTATTCCCCAATGGAGGTATGACATATAATCTTTTCTGCCTTTACTTATCAATTCAC70.16818837097549255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16818837097549255No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG70.16818837097549255No Hit
GTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAAGAGAATTAATGTGCGTATTGAGCACATTAAGCA60.14416146083613646No Hit
GAACAGCTCATAGGAGAGTCATGATTTTGAATCACCCAGATAAAGGTGGATCTCCTTACGTAGTAGCCAAA60.14416146083613646No Hit
GGATTCAAATGGAATGGAATGGAATTGAGTGGATTTGAATTGAATGGAATGTAATGGTATGGAATGGAATG60.14416146083613646No Hit
CAGTATCTACAACTTATTCTTGTAAAAATTTCTAGCTTAAAAGATAAAAATGAAATGCTATCCAAAAGGTG60.14416146083613646No Hit
ATGTAAGAATGCTCCTAAGCTGAGGGAAGAGAAAAAGTGATTAATGGAAAGGGACATAACAGGAAAAAAAA60.14416146083613646No Hit
GATCTGGTGATTTCATTTCAGTTAATGCTATGTGTCCTTGACTATTTTATTAAACTCTCACCTCCCATGTT60.14416146083613646No Hit
GCTCTGTTTAGTTTGATATTAAAGCAAAATTAAGAGGTCTTAGTTTTTCCTATAGAACTTTTAATATGTCA60.14416146083613646No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC50.1201345506967804No Hit
GATTGTTGCTGCTGGTGTTGGTGAATTTGAAGCTGGTATCTCCAAGAATGGGCAGACCCGAGAGCATGCCC50.1201345506967804No Hit
CCTATATGTGCCCCAGCTACTTGGGAGGCTGAGGTGGGAGGATGGCTTGAGCCCGGGAGTTGAGGCTGCAG50.1201345506967804No Hit
GGCTGAAGAAAAGGAAGACAAAGAAGAAGAAAAAGAAAAAGAAGAGAAAGAGTCGGAAGACAAACCTGAAA50.1201345506967804No Hit
GCCAGGAGTCTACCAAATGAAGAAATAGTGCAGAAGATAGAGGAAGTACTTTCTGGGGTCTTAGATACAGA50.1201345506967804No Hit
GTATGGTATGGTTGCACCACTTGGTAAACTTACTAAAAATAATGAATTGTACACTTGAACTGGGTGAATTT50.1201345506967804No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.1201345506967804No Hit
GCCTTGTGATTGCTGCTGCTTTAAAGGATAAAGTACTCTTTGGGGGATGGGTCTGGTTTGTTTTGTTTTAT50.1201345506967804No Hit
GGCTAAGACTGATCCGTCAATCTTGGGTGGAATGATTGTGCGCATTGGCGAGAAATATGTTGACATGTCTG50.1201345506967804No Hit
CCGCATGGATGGGCTTCCCCAAGGATTTATTGACATTGCTACTTGAGTGTGAACAGTTACCTGGAAATACT50.1201345506967804No Hit
CTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGTAGA50.1201345506967804No Hit
ACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTG50.1201345506967804No Hit
AACAGATGCAATTCCCGGACGTCTAAATCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTC50.1201345506967804No Hit
AGTTATGGCTCAACCCAAAGAAGATGAAGAGGAAGATGATGATGTAGTGGCTCCTAAACCACCTATTGAAC50.1201345506967804No Hit
AAGGATGTATTATTGAGAAGTAAGTTGAAGGGCTTATAAGGAAAAATGTTTTATAACTGAGTAATATATTA50.1201345506967804No Hit
TCCTGACACTAAGGAAATGCTGAAGCTTTTGGACTTCGGCAGTCTGTCCAACCTTCAGGTCACTCAGCCTA50.1201345506967804No Hit
CATAAAATCTAGACAAAAAAGGAAGGAATCGAACCCCCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCC50.1201345506967804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)