FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1394.1319857312722945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1203.56718192627824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG541.6052318668252081No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA441.3079667063020213No Hit
GGCACCAAAGTAGTTCTAGATGACAAGGATTATTTCCTATTTAGAGATGGTGACATTCTTGGAAAGTACGT170.5053507728894174No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA160.47562425683709864No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT160.47562425683709864No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGG140.4161712247324614No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAG110.3269916765755053No Hit
ATATTTAGTTTTTCAAACAGTTTGTTGGGCAGCTAATAGTGTGAACCAGGTCATTTTTGTATTGAGTAAAA100.2972651605231867No Hit
GTTTCAGGTGGTTTTAAAGAACACGTGGAAATCCCTTGAATTTAGGACCTGGTTAACCAGAAAGATAAGAC90.267538644470868No Hit
TTCGTAGGATGGGTGAGTCAGATGATTCCATTCTCCGATTGGCCAAGGCCGATGGCATCGTCTCAAAGAAC90.267538644470868No Hit
TTGCAAAGGTATGGGATAGAGGGGTCAGATGCAGATCTCTACTGTAAAATGGGCTCCCTGGTATCTCCTGT80.23781212841854932No Hit
GAGAATACCTGTTTAGTGTTGCACTTTAGACTGGTGTCTGTTTTGTTAATGCAGCTGTGCCACAAATTCTC80.23781212841854932No Hit
GGATTACTTGGCTGCCATAGCATAACAATGAAGTGACTGAAAAATCCAGAATTTCAGATAATCTATCTACT80.23781212841854932No Hit
ACAAAGCAGTGCTTTGTCCCATTGCTGTGATTTTTAGTCAGACTTTACTCAGTCTGAAATGCAATTAACAT80.23781212841854932No Hit
GGTTCATGTTCCTACCTAAGTCCTGGAAACTAAATATATTTTGCCAACTTTGGAAAAAGAATTATTCTTGG70.2080856123662307No Hit
GTAAGGTTTATGTAGGCAATCTTGGAAACAATGGCAACAAGACGGAATTGGAACGGGCTTTTGGCTACTAT70.2080856123662307No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACC70.2080856123662307No Hit
GGATATTGCTGCATATCGAGCTAAAGGAAAGCCTGATGCAGCAAAAAAGGGAGTTGTCAAGGCTGAAAAAA70.2080856123662307No Hit
GAGCAAGCAGAAAGCCAGTAACTTCGCTCTGTTAGAGGTGGAGGATTTTCCTATGGTTCCCCCCATTTCCT70.2080856123662307No Hit
GCAAAAATGTCAATGCAAAGAAGAAATAAAATCTAGACGAATGGAGATGCAGGAGCTGCGGGAGCCGAATT70.2080856123662307No Hit
GGACCAGCCTGGGCAACATGGCAAAACCCTGTCTTTACTAAAAATACAAAAAATTAGCTGGCCATGGGGCT70.2080856123662307No Hit
TCTATCTTCTGAGCCTTCCCCACCGTAAACTATGGACTCTAGTTCAGTTTTATATGCAATGGATCACTATT70.2080856123662307No Hit
GTTTTTAGACCTGTGTTACTAAAAAAAAGATGAATGTCCTGAAAAGGGTGTTGGGAGGGTGGTTCAACAAA60.178359096313912No Hit
ATCCTGTGCTGAAAGAGAAACCAAGAAAAAAGATGACATTCCAGAAGAAGACAAAGGAAATGTCAAACAAT60.178359096313912No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC60.178359096313912No Hit
GTTTACTCAATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.178359096313912No Hit
AGCTGCTAAAAAACGGATCACCGGTTTCCGACTGAGTCTGGGGATTTTGGCCTTGTTGACCCTCCTAGGTG60.178359096313912No Hit
GTATCTTGTGGAATGGGAAGGAGCAACTGCATCCTGACTGTAGGACTGAACATTATGTTCACTGCACTCTG60.178359096313912No Hit
GGTTTGAATGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.178359096313912No Hit
GTAGAATGTGCATGTTGTAGTCCTATGATGATGTAAACTTGGTACTACATAATGACTTGCTCCACACATGT60.178359096313912No Hit
GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGGCC60.178359096313912No Hit
AGTCTGAGATAATACATTTGTAGCCCTCAGCTGGCTGATAGGCAATCACTGCTAAGGTATTAGCTGTTGAG60.178359096313912No Hit
CAGGCTGGGTGGCCCGGAGGCTGGGCTGGGCGAGTATCTCTTCGAAAGGCTCACTCTCAAGCACGACTAAG60.178359096313912No Hit
GCTATGAAAATAGCCGAAGCCAATAAGCTGAAGCTAACCCCTGAATATCTGCAGCTGATGAAGTACAAGGC60.178359096313912No Hit
GTCAAACTGCTTATTCAGGCTAAGTGGGAAATGAATTTGTGCAGTGTGTTCCCTTAATTATCTACTGAATG60.178359096313912No Hit
CATGGTCGGATTAACCCATACATGAGCTCTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGAT60.178359096313912No Hit
GACTTGAATAATATGGGATTTGAGATAATTTTAGAGATTTCAAAAGATAATTACAAATACAGTCATAAATA50.14863258026159334No Hit
TCCAGTATGACCGGACCCTGCTGGTAGCTGACCCTCGTCGCTGCGAGTCCAAAAAGTTTGGAGGCCCTGGT50.14863258026159334No Hit
CTGCCTGCACCCCAAGCTGCAGAGCACCAAGCGCTTCATCAAGTGGTACAACGCCTGGAACGAGAAGCGCA50.14863258026159334No Hit
GCTTTTGCACCCCTGTTTTTTTCACTGCGAGGGAGAAGAGGAGCTCACGAACTTCATAAAGCGTTAGTGTC50.14863258026159334No Hit
CAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATA50.14863258026159334No Hit
CTGATTTCATCCTCAAGTTTCCGCACAGTGCCCACCAGAAGTATGTCCGACAAGCCTGGCAGAAGGCAGAC50.14863258026159334No Hit
CTTTATAGGTCAAGAGCTCTGTTTTAAATACAAAATTTATTGAATATACTTTTTCAAACAAAGTTTCATGT50.14863258026159334No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAA50.14863258026159334No Hit
GTGAAGCTTATCAAGAAGATGGGTGACCACCTGACCAACCTCCACAGGCTGGGTGGCCCGGAGGCTGGGCT50.14863258026159334No Hit
GGGTAGCTGAGTCTGCTTAGGGGACTGCATGGGAAGCACGGAATATAGGGTTAGATGTGTGTTATCTGTAA50.14863258026159334No Hit
CGCCGCCTTTTCCGCTGCGCAGCATCGTTCTTATATGCGATTAACAGAAAAAGAAGATGAATCACTGCCAA50.14863258026159334No Hit
GCTGAACATCAAGGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACT50.14863258026159334No Hit
GGTCAGGAAGGTGTAGTCCTTCAATAGGAAATTGTAATTAAAATATAATTTTATAGAACCATTTTTATGTA50.14863258026159334No Hit
CAGCCCAGAGCAGCAAACACATGAAAGTCAGAAGTCTCTAATATATCATTTGTCTTTTTTCCAGTGAGGTA50.14863258026159334No Hit
AGCGGGGCATCATTGGCTATGATGTTTGAAGACCAATCTTTAACATCTGATTATATTTGATTTATTATTTG50.14863258026159334No Hit
GTTTTAGAGAGATTTTAAAAATATCTTTTAAATTATACTTATGTATCTATTTTGTGATATTTATTAAAAGT50.14863258026159334No Hit
GCACTATGAGTGAAAGAAGTGGAAACAACAAATAATAGAATTAGACCTAGAGACTGTTGGAATTATTAGAT50.14863258026159334No Hit
GATAGAAACAAGATAGAAAATGAAAATATTGTCAAGAGTTTCAGATAGAAAATGAAAAACAAGCTAAGACA50.14863258026159334No Hit
CTATTGAAATGGCATCAACATGATGCTGCCCATTCCACTGAAGTTCTGAAATCTTTCGTCATGTAAATAAT40.11890606420927466No Hit
ACCACAGACCTTCGCCTCGCCCCGCCGGTTCCTCACCCTCGGGGAGCAACATGGATAATCTCAGTGATACC40.11890606420927466No Hit
GAGCCGAGATCATGCCACTGCACCCCAGCCTGGGCAACAGAGCAAGACTCCATCTCAAAAAGGAAAAAAAG40.11890606420927466No Hit
GATTATTTTATTGCTTCAAAAGCAGTGGACTGTCTTTTGGATTCAAAGTGGGCAAAGGCCAAGAAAGGAGA40.11890606420927466No Hit
ATATCTGGCTGTCCTTTTTATAATGCAGAGTGAGAACTTTCCCTACCGTGTTTGATAAATGTTGTCCAGGT40.11890606420927466No Hit
GCTTGTAATATGTTGTGTTAGAAATGCATATTTTATTCAGCTTTTTTTCTGAGCTGGCTCCAAAAAAAAAA40.11890606420927466No Hit
GATTTGGGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11890606420927466No Hit
TCTTAATACTCACCAATAAATTCTACTTCCTGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11890606420927466No Hit
GGAAGAAATTGAAATCCTGAACAGACCAATAATGAGTTATGTAATTGAATCAGTAATAAAAATTCTACTAA40.11890606420927466No Hit
TGTTATGATAGGACATAGTAGTAGCGGTGGTCAGACATGGAAATGGTGGGGAGACAAAAATATACATGTGA40.11890606420927466No Hit
GACTCAGACAAGGATGAAACAGGCGCCTATTTAATCGACAGAGACCCCACCTACTTTGGGCCTGTGCTGAA40.11890606420927466No Hit
GTGATAATGAGTAAACTAAAACTATTTTCAGCTTTTCTCAATTAACATTTTGGTAGTATACTTCAGAGTGA40.11890606420927466No Hit
GAATTATTTTCATTACAACCAGAGTTATCCTCCCACATACAATGTGAAGGACATGCTTGTGCCGACTGCAG40.11890606420927466No Hit
GATCCATTTCATAAAGTATGATTTGCCCAAACCTGTACCATTTCCGTATTTCTGCTGTAGAAGTAGAAATA40.11890606420927466No Hit
GTCTTGGTGTTAGTCTCCTGCACTCATTAATTTGTGTTATTGTGGATGAAAATATTTACTCTTATGCATTA40.11890606420927466No Hit
CTGCTACACCTCAGCCCGGGGCTGCACTGCCACCCTGGGCAACTTCGCCAAGGCCACCTTTGATGCCATTT40.11890606420927466No Hit
GTGGACAACACATTTCAAAGAAGATCAAGTTTGTACATAACACTAGAGGCATTTCTTATCAAAAGGATTGG40.11890606420927466No Hit
CCTTCTGAGGCACCTCCAGCTGCCCCCGGCCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTGTGAT40.11890606420927466No Hit
GTTAAGTTGTGATCAGTTTAAAATAATGGGTTATAAGATGTTATTTGCAAGCCTCATGGTAACCTCAAATC40.11890606420927466No Hit
GCCATGAAGAACTACACGTAACATCATCATTTGTATTAATTGCACAACTCCAATGCTAAAGGTTGGATTGT40.11890606420927466No Hit
CAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTAT40.11890606420927466No Hit
GCTTTTTGGGGTAATTTGAATTACTTTTTTAATGACTGGGGTTCCATTTGACTGTTTGCATTGAGATTGCA40.11890606420927466No Hit
GTTCTAATAATGTCCTTAAATTTTATTTCCAGTTCCTGTTCCTTGGAAAATCTCCATTGTATGTGCATTTT40.11890606420927466No Hit
GTTTTGTATAAATTTGTCATGTTGAACAGCATTTTAGCATGGTAAGTTCCCTTAGCTATATGAATTTTGGC40.11890606420927466No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACGCTATAG40.11890606420927466No Hit
CCCCATCTGGCTGACAGCCTCTGCAGTCCGTGGGCTGGCAGTTTGTTGATCTTTTAAGTTTCCTTCCCTAC40.11890606420927466No Hit
GGTTAATGACTTATGAGCAAGCTGGTTTGGCCAGACAGTATACCCAAACTTTTATATAATATACAGAAGGC40.11890606420927466No Hit
GCCTGGTTTTTCTCAATACGCCTTTAAAGGTTTTTAAATTGTTTCATATCTGGTCAAGTTGAGATTTTTAA40.11890606420927466No Hit
GGTATTTTCTGTGGCCCTGGGAGCTGTCTGTCTTTCCCTTACCCCCAAGGATGCCAGGAAGACGTCCACCA40.11890606420927466No Hit
ATATTATAGTGATAATTTTGTATTTTCACAAAAAAAAAGTTAAACTCTTCTTTTCTTTTTATTATAATGAC40.11890606420927466No Hit
ACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAA40.11890606420927466No Hit
CCTAATTAAGGTTGATGACAACAAGAAACTAGGAGAATGGGTAGGCCTTTGTAAAATTGACAGAGAGGGGA40.11890606420927466No Hit
TTTTTGAGTTTATTCTGATTGATTTTTTTTCTTGGTTTCTGGATAAACCACCCTCTGGGGACAGGATAATA40.11890606420927466No Hit
AGAATTAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11890606420927466No Hit
ACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCT40.11890606420927466No Hit
GATTCAAATCCGCTGACTGAAATACTTAAGTGTCCTACTAAAGTGGTCTTACTAAGGAACATGGTTGGTGC40.11890606420927466No Hit
GCATCTGAATCTTCAGAAGATAACTGTCACCCTTTTTACCCATTGCTTGGAAGTGGATTGTGATCCTTGAT40.11890606420927466No Hit
GGTGGGAGCCCTCACAGGCAAGGGGGCTGTTGGATTTCCATTTCAGGTGGTTTTCTAAGTGCTCCTTATGT40.11890606420927466No Hit
CTTTAGTTGAACCCAGGTACCTGGGTTCAACTAAAGCGCCAGCCTGCTCCACCCAGAGAAGCACACTTTGT40.11890606420927466No Hit
GTTAATACAAGGCAAATCATATCTTGGATCAGAGGCAGATGTTTGGAGCATGGGCATACTGTTATATGTTC40.11890606420927466No Hit
GCGGGCAGGCCGGGAGTGTACTGTGTGCAGCTGACCCAAGGCAGCCACATCTGCGTTTGTCCTTTGAGAGG40.11890606420927466No Hit
GATTGCAAGGGAGCATGGTATTAGGTTTTTTGAGACTAGTGCAAAAGCAAATATAAACATCGAAAAGGCGT40.11890606420927466No Hit
TCTTCCAGTTGCACTATTCTGAGGGAAAATCTGACACCTAAGAAATTTACTGTGAAAAAGCATTTTAAAAA40.11890606420927466No Hit
CTGCCCAAATGTCAATCTTAATAGATGAATGGATAAACAAAATATCTGTATGTACAATGGAACATTTTCCA40.11890606420927466No Hit
CATCTTGGTCAGATTTGAACTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATAAG40.11890606420927466No Hit
GCTTCCTAGATAGAAACCAAAGCAGTGCAAGATTCAGTTCAAGGTCCTGAAAAAAGAAAAACATTTTACTC40.11890606420927466No Hit
GCTGTCCTCCATCCCACCCCTCTTACTCCAGCCTCGTGTTGTAAATAAAGTTTCGGTGGTCATGCTGAAAA40.11890606420927466No Hit
GTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAA40.11890606420927466No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGC40.11890606420927466No Hit
GCAGCGAAATGGGCATCAGCCGGTCAGCAGTGCTGGTGGTCGCCTACCTGATGATCTTCCACAACATGGCC40.11890606420927466No Hit
GCATGCACGGGCAATGACGTCCCAGGGACATGGTCTTCCTCCCTCCCACTTCAGCTCTTCCATACTCCTCC40.11890606420927466No Hit
TGTAATTTTGTATCTTGATGTCTTTGTTAAGACTGCTGAAGAATTATTTTTTTTCTTTTATAATAAGGAAT40.11890606420927466No Hit
ACTGATCAGTTTTGAGAGATCGTTAATGCCCTTGAAGTGGTTTTTGTGGGTGTGAAACAAATGGTGAGAAT40.11890606420927466No Hit
CCTCAATGTATTTTAAATCTGTTGCTGTCATCATTAACGGTATATGACATATAAAAGCAAGTTAAAATTTA40.11890606420927466No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11890606420927466No Hit
GTGCATGAGCGAGATGAGGCAGCCTATGGGGAGCTCAGGGCCATGGTGCTGGACCTGAGGGCCTTCTATGC40.11890606420927466No Hit

[OK]Adapter Content

Adapter graph