FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8848
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.5650994575045208No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG490.5537974683544303No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAG300.33905967450271246No Hit
CTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGT270.3051537070524412No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATC230.25994575045207957No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAGGAAGGAATCGAACCCCCCAAA170.19213381555153705No Hit
GTGATTGACTTGACTTTCTAGCTTCCCTTGTCCGGAGGATATTAAAATGCTAGGGTGAGGTTTAGCCATCT160.18083182640144665No Hit
TCCCTACACGACGCGGGCTCAGAATCTAAATTGGACTTCTGTTGTATTCTCACCACTCAATTTGTTTTTTA150.16952983725135623No Hit
AAACCTGACTTTTCTTCAAAATCACCTATTGTATCAGTTATCTATTGCTGCAAGAGGACAGTAGGTGAAGA150.16952983725135623No Hit
CCTTTGTCCAATGTGAACATTTATTCATATTGTTTTGATTACCCTCGTGTTACTACAAGATGGCAATAAAT140.15822784810126583No Hit
GTAGTAAATAGTTCTTTGTAACCTGTGTGAAGCAGCAACCAGCCTTAAAGTAGTCCATTCTTGCTAATGGT140.15822784810126583No Hit
ATATCAGTGTAAATGAGATTAATGAGCCGTGTGGCAATTCCACGAATCTATCCCCTTCCACTCAGTTTCCT140.15822784810126583No Hit
ATTCCATAAAGCTATCAGAAGAAATCCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGGGAGA130.1469258589511754No Hit
ATATATTTTTTTCAGAGAAAGTTATTCGCTCGATTTTTTCTGAATCATAATTAAACTTTATGACAAAAAAA130.1469258589511754No Hit
GCTTTTGACTCTGTATTAACATCTGAGAAACCCTGAATCCTGCAATCAAGTAGAAGTCAACTTCATCTGAA130.1469258589511754No Hit
TCTTTATTGAGCATTTTGGAGACTGAGTTTTTAATATCTGTATATAAGTTGTGTATTTTAAGAATAAATTA130.1469258589511754No Hit
TATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAAAAAAA130.1469258589511754No Hit
CATAAAAGGGCATCAGAAAAATACAAAGACAACCCATCAATATCAGGAGCCTGAGCATGAGTTAAAGCATG120.13562386980108498No Hit
GGATGCTTAGTCATGAATAAATCTGGGAAACGGATTCCTTCCACCTTGGTAATGTTAGTGAGACAAAATAT120.13562386980108498No Hit
GTCTCCTTCAGCACGGCGTGGTGTGTCCCTGGACACAGCTCTTCATTCCATTGACTTAGAGGCAACAGGAT120.13562386980108498No Hit
GTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAATTGAAAACAAAAT120.13562386980108498No Hit
ATATTATACTACTCTAAATAGCATATTTCATCCAAGTGCAATAATGTAAGCTGAATCTTTTTTGGACTTCT120.13562386980108498No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC120.13562386980108498No Hit
ATCACTCTTCATGTGTTCAGAATTACTGTTTTTTTTGTTTGTTTTTCCTTTTTGTCACTGTGTACATTAAA120.13562386980108498No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT120.13562386980108498No Hit
GTATGAACTTAGTGGCAGAATAGAGATGGCAATGGAAGAGTCAGTGAACCTGAAGATAGATCAGTATGGAT120.13562386980108498No Hit
GTCTAGTTGACTTTAATGAATCTTTGGTGTGGAATGAAAAATGTGAACTTCTTACTCATAGTGTGGTTTTT120.13562386980108498No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCC110.12432188065099457No Hit
GTGTAGCACAAGTTATCTGCCAGTGCTAGTTAGCAAACAGAATGGAGCCAAAAAAGAATTGATTTGGGAAG110.12432188065099457No Hit
ATAAATAGCTGTATGAAATTTGTTTCCTGAATATTTTTCTTCTCTTGTACAATATCCTGACATCCTACCAG110.12432188065099457No Hit
TATTATGAGTAAACTCCGAAAATTTTGTTTATCCAAAGGCTCAATGGATTATGTTTCTATTATATACAAGG110.12432188065099457No Hit
GGTGTCTACCATGATTATTTTTCTAAGCTGGTTGGTTAATAAACAGTACCTGCTCTCAAATTGAAAAAAAA110.12432188065099457No Hit
CTATAACGCTGCTTTAGAATGAAGGTCTTCCAGAAGCCACATCCGCACAATTTTCCACTTAACCAGGAAAT110.12432188065099457No Hit
GGTTACTACTAGTATCTACCTCAAAGCGTTGTCATGTGGATTGAGATGATATGTATGAATCACATAAAAGA110.12432188065099457No Hit
ATACAGAAGGCTATCACACTTGTGAAATTCTCTTGTCTAATCTGAATTTGCATTCCATGGTGTTAACATGG110.12432188065099457No Hit
ACAATGGCCAACTGGACATCATCGTGGCAGCTGCCCTGACAGAGCGCTCCTTGATGGGCATGGACTGGAAA110.12432188065099457No Hit
GATAATCATTGCTTATGTCAAATGGAGCTGAATTTGATAAAAACCCAAAATACAGCTATGAAGTGCTGGGC110.12432188065099457No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC100.11301989150090416No Hit
GTACTTAGCTGTACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTT100.11301989150090416No Hit
TGAATGAACAGATTGCAAGATTGGAACAAGAAAGAGAAAAAGAACTGAATCGTCTAGAGTCGTTGAGAAAA100.11301989150090416No Hit
GCCCCTAGTCCGGGGTAGCCTAGGAGGCCGACCCTCTTTGGAGTCCTGCTGTCTGGGTGCCAGGGCCGGAA100.11301989150090416No Hit
AGTCATGTCTGCTTACAGGTGTTATTTGTCTGTTAAAACTAGTCTGCAGATGTTTCTTGAATGCTTTGTCA100.11301989150090416No Hit
CATTGTGTCCTTATTATTAGTTATATAAAACTATGCTTTAGACTTTGTTAGAAACTTCTGCCCCACCTTGA100.11301989150090416No Hit
ATTGATCTCCATACAAATGTTGCCACTGCTGTTTTAGAACATATAAAGGCAAGAAAATTGGATGTATATTT100.11301989150090416No Hit
ATATAAAACAATTACAAAATGAGTGTTGTTTGTAAAACAGTAACATCAAATTGGCTAGAGAGATAAATGTA100.11301989150090416No Hit
GCAGCGGAAGGAGCGGCTGTACCCGCTGCGGAAGTACGCGGTCAAGGCCTGAGGGGCGCATTGTCAATAAA100.11301989150090416No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA100.11301989150090416No Hit
TATCTACTTACAGAAAAGTAAATGAGACATAGATAAAATAAAATCACACTGACATGTTTTTGAGGAATTCA100.11301989150090416No Hit
CTTAGACTCGAAGTTTGATAAAAGTACCTTTTCATGTGGTGACAGTTGTGTGTTGATTGGCTAGGTTTCTC100.11301989150090416No Hit
GTTCAGGTTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAA100.11301989150090416Illumina Single End PCR Primer 1 (100% over 58bp)
TGCCTGTTCATATCCTTCAGTGACATTGAGGAAGCAGTGTTTCTCTTTTTAAAGGAGAATAGTTGTCAACC100.11301989150090416No Hit
GTTGAGAAGGATACAGTTTGTTTTTAGATGTCCTTTGTCCAATGTGAACATTTATTCATATTGTTTTGATT100.11301989150090416No Hit
GTTACGGAAGCGGCGGGAGGAGAAGCTCCTTCGCGACGCCCAGCAGAACTCCTGAGGCCTCCAAGTGGGAG100.11301989150090416No Hit
ACACAATTCCTTACAGAGCACTTATTAAAAAAAAATCTTAAGAGTTAAAAAAAAAAAAAAAAAAAAAAAAA100.11301989150090416No Hit
GAGCTGGAGAGGGAGCTTTTTAAGCTCAAGCAAATGTTTGGTAATGCAGACATGAATACATTTCCCACCTT90.10171790235081375No Hit
GATGAAAGTGTCATGGCACAAAACAAACAGCAATAAAGATGTAGCCATAACACAAAGAATAAAAAGAAGGG90.10171790235081375No Hit
GTGCAAACTTGTCGGGAGGAGCATAAGAAGAAGCACCCAGATGCTTCAGTCAACTTCTCAGAGTTTTCTAA90.10171790235081375No Hit
GATTGGGAATGGTGCTTTTGCCAACTTAAAAGGATTAAAGTAACGGAGATATACACAAATTTTAAAATTAT90.10171790235081375No Hit
GTTCTATTGGATAGTACCATAATCTTTTTATAGTAACTTGGAAATACTATTTGATATTAGATGTTAGACCA90.10171790235081375No Hit
CAAAGAAACATGTCAGCAGGGCCTATGGTGGTTCCATGTGTGCTAAATGTGTTCGTGACAGGATCAAGCGT90.10171790235081375No Hit
ATTCATATGTTAGCATATAGTTTCTTTGCACCCACTATTTATGTCTGAATCATTTGTCACAAGAGAGTGTG90.10171790235081375No Hit
TTCCCTACACGACGCGGGGGAAATGTTTTCTGATAAAACAAACATTTAGTTGAGGTTCTTGATATAAAACA90.10171790235081375No Hit
CTATATGTATTCCTGCCTAAATAAATCATCTATTAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10171790235081375No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC90.10171790235081375No Hit
CCCTACACGACGCGGGGAAGCAGATTTGGGTACCACCCAAGTATTGACTCACCCATCAACAACCGCTATGT90.10171790235081375No Hit
AGGTATCTCTAAGGCACATAGTAGAAAACAAAATTGGTTAATTACTCAAGTTCCTTTCACTGTGATTTGGA90.10171790235081375No Hit
AGGTATCCTCTGTTTGAAGGGCAAGAGACTGGTAAAAAAGAGACAATCGAGGAATGAGGACAATTTTGACA90.10171790235081375No Hit
GTGAGGGACGCCACTGCGGCCACCCGGCTTGCTGCCTTCCTGGGCGCCACTCCCCCAGGCGACCCGACGCG90.10171790235081375No Hit
CATCTAAAGGAGATCATATCTTACAGGCAGCTACATGTTATGGATGAAACACATGTGATTAATCAAGTGAA90.10171790235081375No Hit
CTTTTAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10171790235081375No Hit
GAACTCTCTGGAACCATTAAAGAGATCCTGGGGACTGCCCAGTCAGTGGGCTGTAATGTTGATGGCCGCCA90.10171790235081375No Hit
AGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCCTGCAGTGAGCCGAGATTGCTGCACTGCAC90.10171790235081375No Hit
CCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTCCCCCTCAAACCAAAAAA90.10171790235081375No Hit
CTCCAAGAGTGTGGCCCGCCGGGTCCTCCAAGCCCTGGAGGGGCTGAAAATGGTGGAAAAGGACCAAGATG90.10171790235081375No Hit
GGGAGGAAAAGTAACAATAGTCCACATGTCCCTGGCATCTGTTCAGAGCAGTGTGCAGAATGTAATGCTCT90.10171790235081375No Hit
CCCGTATACATAAAATCTAGACAAAAAAGGAAGGAATCGAACCCCCCAAAGCTGGTTTCAAGCCAACCCCA90.10171790235081375No Hit
CTTCAGACTCTGGTTACAGATTGTCTTGATCTCTTGGATCTCCTCAGATCTTTGGTTTTTGCTTTAATTTA90.10171790235081375No Hit
ATTTACACATTGTTTTATTCACCGTGAACGTTTAGTGGCAGAAAAGTTGTCTCCATGTTTACATAAAATTC90.10171790235081375No Hit
TGATGGCACCTTCCTAAACCCTCATGGGTGGTGTCTGAGAGGCGTGAAGGGCCTGGAGCCACTCTGCTAGA90.10171790235081375No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACC90.10171790235081375No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATT90.10171790235081375No Hit
ATACAGTATATTGTCATGTGAAAGTGTGGAATGGAAGAAATGTCGATCCTGTTGTAACTGATTGTGAACAC90.10171790235081375No Hit
TCCTGACACTAAGGAAATGCTGAAGCTTTTGGACTTCGGCAGTCTGTCCAACCTTCAGGTCACTCAGCCTA90.10171790235081375No Hit
GGGAGGACCAGATCAACAGGCTTATTAGAAGAATGAACTAAGGTGTCTACCATGATTATTTTTCTAAGCTG90.10171790235081375No Hit
GAAATAAACTGTAGAGTTCCATATTCTGGTATTGTGATTATATTGTTTTATATCAAAAAAAAAAAAAAAAA90.10171790235081375No Hit
CTCTTCAAGAAAATGAGGAGGAGGAGATTGGGAACCTAGAGCTTGCCTGGGATATGCTGGATTTAGCAAAA90.10171790235081375No Hit
GCAGTGGGACCAGCAGAGGCTTCGTGACGGAGTTATCAGAGACATTGAGAGGCAAAAAAAAAAAAAAAAAA90.10171790235081375No Hit
CAAAGAAGCTTTTTCACTATTTGACAAAGATGGTGATGGAACTATAACAACAAAGGAATTGGGAACTGTAA90.10171790235081375No Hit
CTTTTTTACTCATTGAAATGTCACGTACTGTCTGATTGGCTTGTAGAATTGTTATAGACTGCCGTGCATTA90.10171790235081375No Hit
ACAGAGGAGAAGAAGCCTGCTGCATAAACTCTTAAATTTGATTATTCCATAAAGGTCAAATCATTTTGGAC90.10171790235081375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)