FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290
Sequences flagged as poor quality0
Sequence length71
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26820.775193798449614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.9302325581395349No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG80.6201550387596899No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT80.6201550387596899No Hit
CCTGTTTGAAATCATCTCCCAAGCCATGCTGAATGCTGTGGACCGGGATGCAGTGTCAGGCATGGGAGTCA70.5426356589147286No Hit
ATTGTAATGTATTCTAAATTAATGCAGAACCATATGGAAAATTTCATTAAAATCTATCCCCAAATGTGCTT60.46511627906976744No Hit
CCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAA50.3875968992248062No Hit
GATGAGAAGAAGGAGGAGTCTGAAGAGTCAGATGATGACATGGGATTTGGCCTTTTTGATTAAATTCCTGC50.3875968992248062No Hit
TTTCGCAAAAAGCATTTCACTTGAACCTCTGCAGAAAGAACTGATTCCTCAGCAGCGTCATGAAAGCAAAC50.3875968992248062No Hit
GTGCCAGTATTTTAAAATAGCGTTCTTGTAATTTTACACGCTTTTGTGATGGAGTGCTGTTTTGTTATATA50.3875968992248062No Hit
GTTTTTGAGAAGGAATGAAGTGGGAACCAAATTAGGTAATTTTGGGTAATCTGTCTCTAAAATATTAGCTA50.3875968992248062No Hit
GGACTGCCTTGCTCACCTGTCCCCTCGGCGCGGCCCCGGGGAGCTCCCGAGAGGCCCCCGGGATCGCTGGC50.3875968992248062No Hit
GCTTTGACTCCAGGATCCCTGGAGTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGA50.3875968992248062No Hit
GAAGAAGACCTGGTCCCTCTGGAGGTTGCAGTGGCTGAAGGATGCATCATGTGCTCCTACCCTGCTCTACC50.3875968992248062No Hit
ACGCGGGGATATGAAAAACCATCGTTGTATTTCAACTACAAGAACACAAAAAAAAAAAAAAAAAAAAAAAA40.31007751937984496No Hit
GGGGAGACAGAGCGAAACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.31007751937984496No Hit
GCTGTGAAGAGACTCTGTTTGCTTGTGTTTGTTTGTTTGCAGTCTCTCTCTGCCATGGCCTTGGCAGGCTG40.31007751937984496No Hit
GGGATATATATTTAATTTTCTTAAATTCCCGTTAAACTCTATTTTATGGTTTTGATTTCAGATTGCAAACA40.31007751937984496No Hit
GAGGAGAAGAAAGATGAGAAGAAGGAGGAGTCTGAAGAGTCAGATGATGACATGGGATTTGGCCTTTTTGA40.31007751937984496No Hit
GTCTCTGGTTCTATAGTGCAAATGCTTTTATTAGCCAATAGGAATTTTAAAATAACATGGAACTTACACAA40.31007751937984496No Hit
TCCCTACACGACGCGGGACTACAGCGTTGGGTCAAGGGGCCTTCCTGCCCGCAGAGTTGTCCTTGCAGCAT40.31007751937984496No Hit
GCCCCTGTGGCTGCTGCCACCACAGCTGCTCCTGCTGCTGCTGCAGCCCCAGCTAAGGTTGAAGCCAAGGA40.31007751937984496No Hit
CTGGATGACAGACTGAACCCCATCTCAAAAAATTAAAATAAAATAAAATAAAATAACTATAAAAAAAAAAA40.31007751937984496No Hit
CGATACCAAGTATTTTCTTTGACAAAATGGGATGAAGTGAGAAATCAGTAACAGAAGGAAAACTGGAGACT30.23255813953488372No Hit
GTATTTATAGTAACGTAAGCTATTAATGCTAACTCTTGCATGTCAAGAAACATTAGTCTTAGGAATTCTTC30.23255813953488372No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA30.23255813953488372No Hit
GTGATGGCATTACTCTGCACTATAGCCATTTGCCCCAACTTAAGTTTAGAAATTACAAGTTTCAGTAATAG30.23255813953488372No Hit
CCTTCGGAAAGAGAGAGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTGGCAGCT30.23255813953488372No Hit
GCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGATGACATGGGCTTTGGTCT30.23255813953488372No Hit
GTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCAG30.23255813953488372No Hit
TCCCTACACGACGCGGGTAATAGCTATTGGTCTTCAAGTGGGTTTAGATTTGGTGACATCAGTTTGATATT30.23255813953488372No Hit
GCCACCACTTTATAACCAGATGGTTCCTTTCACAACCCTGGGTCAAAAAGAGAATAATTTGGCCTATAATG30.23255813953488372No Hit
CCTGTATTCCTAGCACTTTGGGAGGCCGAGGCAGGCGGATCACTTGAGGTCAGGATTTCGAGATCAGCCTG30.23255813953488372No Hit
GCATCTGATTACCTTGAATTGGATACAATTAAAAATCTCGTCAAAAAATATTCACAGTTCATAAACTTTCC30.23255813953488372No Hit
CCTTCAAAGCTAAAATATAAAACTATTTGGGAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.23255813953488372No Hit
ACACGACGCGGGGGGCCAAAAGCTTAGTGTTGTGACCTGTTTTTAAAATAAAGTATCTTGAAATAAAAAAA30.23255813953488372No Hit
ATGTGCGTGTGATTACCAGAGAACTACTAAAAAAACCAACTGCTTTTTAAATCCTATTGTGTAGTTAAAGT30.23255813953488372No Hit
GTTCCCATGCCTGGAGAAGCTAATGCCAACTCATCATGTGATAATTCAATTTGTACAATAAATTATGAACC30.23255813953488372No Hit
GATGACTTACACAGACCATCTACAACATAGCTGAATTTCCTGACATGTTCTATACTACCTCTTTCTTAAAG30.23255813953488372No Hit
GCACACCAACATGGCACATGTATACATATGTAACAAACCTGCACATTGTGCATATGTACTCTAGAACTTAA30.23255813953488372No Hit
CTTCTTGGTGTCATTGCTCAAGAAAAACTGGCAGAATGTCCGGGCCTTATATATCAAGAGCACCATGGGCA30.23255813953488372No Hit
ACGCGGGAAAGAAAACAAAACAAAATCCTCCTACACAAATGAGTCCATTAACCAACTTCTGTAATACAGAA30.23255813953488372No Hit
GTACTCCACACTCCAACCTAGGCGACAGAGCAAGACTCTGTCTCTTTAAAAAAAAAAAAAAAAAAAAAAAA30.23255813953488372No Hit
GGTGTGAGGGACTGGAGTTCGAGGTCAACCTGGGCCCCATTGGTAGGAAAAAAAAAAAAAAAAAAAAAAAA30.23255813953488372No Hit
AACTTTTACTTTGTAGATAATATAAAATAAAAACTTAAAAAAAATTTAAAAAATAAAAAAAGTTTTAAAAA20.15503875968992248No Hit
GACTGAAGACAGGCTATTCTCTGGAGAAAAATAAAATGGAAATTGTACTTAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GGTTTCAGTGAGCCTAGATCATGCCACTGCACTCCAGCCTGGGCAACAAGAGTGAAACTCCATCTCAAAAA20.15503875968992248No Hit
AGAGAAAGGTACCTGGGTTCAACTAAAGCGCCAGCCTGCTCCACCCAGAGAAGCACACTTTGTGAGAACCA20.15503875968992248No Hit
CAGCCCTTGAATGTGAAGTGTCATTGGCATGTCTGGCAGTAGTCTCTCATTAACTCCTCAATAAACAACAT20.15503875968992248No Hit
CTCCAGCATTCCCCCTCAAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GGACTCTCGACCCAAACTGCCCCAGACCCTCTCCAGAGGTTGGGGTGACCAACTCATCTGGACTCAGACAA20.15503875968992248No Hit
GCAGAGAAAGCAGGGGGTCCACAGTCATGAAGGTGTGGAAGCACATGGCATGTTCAGGAAATGCCGGAATG20.15503875968992248No Hit
GGTCAAAAAGCTCCAGCCCAGAAAGCACCTGCTCCAAAGGCATCTGGCAAGAAAGCATAAGTGGCAATCAT20.15503875968992248No Hit
GTATCGAATACCTTTCTTGCCCATAAGGGTTTTTACAAAGAACGTGTCTCACTAGGAACTAGGACACTCAT20.15503875968992248No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAAGTAATTCCCCTAAAAATCT20.15503875968992248No Hit
GGATGGTGAAGGGGGACCTGGTACCCAGTGATCCCCACCCCAGGATCCTAAATCATGACTTACCTGCTAAT20.15503875968992248No Hit
GGCCCTCAAGCCCAAGAGCCATCCCAGGCCTGTTCAGGCCTCAGCTAAGCCTCTCTTCATCTGGAAGAAGA20.15503875968992248No Hit
ACGCGGGCGAGGGAAAATAAATACAGTGATATTCTTACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GTTTCTGTATGTTAATGACTTGTTGGGTAGCTATTAAGGTACTAGAGTTGATAAATGTGTACAGGGTCCTT20.15503875968992248No Hit
CCCACCACCTAGTGTAAAATAAAATCAAGTAATACAATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC20.15503875968992248No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
ACACGACGCGGGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GCCAGAGGGAGGGCTGTTATTTCCAGAGGTGCGCTTTTTATGTACTTTTAGCTAGATGTGGCATGCATCTG20.15503875968992248No Hit
GTATTTCAATCATCTGTAATTAAAATGATCATATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
TCATTGAAGTGGACGATGAACGCAAACTTCGTACTTTCTATGAGAAGCGTATGGCCACAGAAGTTGCTGCT20.15503875968992248No Hit
GAGACAGGAGGATTGCTTGAGCCCAGGAGGTCACGACTGCAGTGAGTTATGATCATGACACTGCACTCTAC20.15503875968992248No Hit
GGTGCATTTTACCTTTTGACAAGGAGACTGGCTTTCACAGAGGTTTGGGTTGGGTTCAGTTTTCTTCAGAA20.15503875968992248No Hit
CGACGCGGGAGTCTGAAGATGGCGGCCTCAGCAGCGCGAGGTGCTGCGGCGCTGCGTAGAAGTATCAATCA20.15503875968992248No Hit
GAAGACTATTCTCAGCAATCAGACTGTCGACATTCCAGAAAATGTCGACATTACTCTGAAGGGACGCACAA20.15503875968992248No Hit
GAGGAAAATACAGAACAGTGTGTATATGGTATAGTTTTTGTATTAAGTCAGGGGGAGAATAAGACCATACA20.15503875968992248No Hit
ACACGACGCGGGAAGGGGAAAAAAAACAAAAACAAAAACTTACGATGCACTTTTCTCCAGCACATCAGATT20.15503875968992248No Hit
TAATAAACAGTCTACAGACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GCACTTTGGGAGGCTGAGATGGGAGGATCACCTGAGGTCAGGAGTTCGAGACTAACTAGCCCCTGGTCAAC20.15503875968992248No Hit
GGGCCCGAGAGCCCTGAGACAGCTGAGCCTGGCCTGCCTGGTGACACGGTGACCCCCCAGCCTGACTGTGG20.15503875968992248No Hit
GGCCAGGCCCCCACTTGGCTTGAAGGGACATTTTCAGACTTTTCTTTCTGTCACTTGGAGTGTCTATGCCT20.15503875968992248No Hit
AACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGTA20.15503875968992248No Hit
ACATTCAAATGTGTGCATTAAATATGCTTCCACAGTAAAATCTGAAAAACTGATTTGTGATTGAAAGCTGC20.15503875968992248No Hit
GCCACCGATATTTCTCGCTAAGAAGACAGAGGAAGCAATCCATGGGAACTACTTATCCACAGTTAAACAAG20.15503875968992248No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAG20.15503875968992248No Hit
GAGGCTCTGCTTCCTTAGGTTACAAAACAAAACAGGGAGAGAAAAAGCAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GCTCTAGTGACTGAGTTCATCACAGCAGTGACTTGTAGCAGCTTCATTTAAAAACAGAGCTAGGCTAGCCT20.15503875968992248No Hit
AGAATGGTGTGAACCCAGGAGGCGGAGCTTGCAGTGAGCCAAGATCATGCCACTGCAGTCTAGCCTGGGTG20.15503875968992248No Hit
ACATGTTCATGTTCACCTCTCATTCACCTGTTTTATATGGTTTAAAATTCTCTTTAACAAAATTCAGAAAA20.15503875968992248No Hit
ATAAATCATATTGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GCTAGTTTCAGTAATTTGCCAGGGCTTCGTCTAACACAAAACAGCCAGTCGGTACTTAGCCACCTGATGAC20.15503875968992248No Hit
GAATAAAAAGTATCCTTTGAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
CCATTGCACTCCAGCCTGGGTAATAAGAGTGAAATTCCATCTCAACAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
CCCTCAGGAGGGGAACGTGGTAAAACCCAAGACATTAAATCTGCCATCTCAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
CCCTACACGACGCGGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGG20.15503875968992248No Hit
GCCCAAGGACGGCTCTGTAATAAACACTTTTGAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GTACTAAAAAATGTACTAGATCATTAAGACTTATGTGCTCTTACTGATTGAAAGATTTTTTATGTTTTCCT20.15503875968992248No Hit
GCTCCAGGGCTACCTGCGATCTGTGTTTGCTCTGACGAATGGAATTTATCCTCACAAATTGGTGTTCTAAA20.15503875968992248No Hit
GGGAATTCGGGAAAATAACCAGTTAAACCAGCTACTCAAGGCTGCTCACCATACGGCTCTAACAGATTAGG20.15503875968992248No Hit
CTTCTGTGTTCTCTGCAGTAAACATTGATTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
ACACGACGCGGGGAAAAAAGCAATATACTAAAATATATATACACAGAATGGTTGCTGATTCTGTAAAAAAT20.15503875968992248No Hit
GGTGGATCACTTGAGTTCAGGAGTTTGAGACCAGCCTGGCCAACATGATGAAACCCCATCTCTACATCTCT20.15503875968992248No Hit
GGAATGTACATCTACCTAATAACTTTCTAAAATTTAAATATGTATAATAAAATAAATGTTTTAAATATAAA20.15503875968992248No Hit
GAGGTGGGAGGATCGCCTGAGTCCAGCAGGTCAAGGCTGCAGTGACCTGATTGTGCCACTGCACTCCAGCC20.15503875968992248No Hit
GCTTAGGAGGCTGAGGCAGGAGAATCGCTCGCACCTGGGAGGCAGAGGTTGCAGTGAGCCAAGATTGCGCC20.15503875968992248No Hit
ACACGACGCGGGAGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GTTACTTGCTTTGAGTTGGAAGCGGTTTGCATTTACGCCTGTAAATGTATTCATTCTTAATTTATGTAAGG20.15503875968992248No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAA20.15503875968992248No Hit
GTGGTGTCTGGAGCAGCTGCTCACCAGCCCCTCACCCAGCTAGGCAGCCACAGACCTGGGACACCTCAGCC20.15503875968992248No Hit
GCTACTCGCGAGGCTGAGGAAGGAGAATCGCTTGAACCTGGGAGGCAGAGGTTGCAGTGAACTGAGATCAC20.15503875968992248No Hit
GAGCTGACGATGAAGTTGATGTGGATGGTACAGTAGAAGAGGATCTGGGTAAAAGTAGAGAAGGATCAAGG20.15503875968992248No Hit
AGGTTGCAGTGAGCCGAGATCGCACCACTGCACTCCAGCCTGAGCAAGACTCCATCTCAAAAAAAAAAAAA20.15503875968992248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)