FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50991
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.6020670314369202No Hit
CTTATATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA2130.4177207742542802Illumina Single End PCR Primer 1 (100% over 58bp)
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG2030.39810947029868016No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGA1520.29809182012512014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1450.28436390735620015No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.2608303426094801No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAG1280.2510246906316801No Hit
AGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAG920.18042399639152007No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG850.16669608362260008No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.15492930124924006No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAG730.14316251887588005No Hit
ATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTA670.13139573650252007No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCT610.11962895412916004No Hit
GATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTT590.11570669333804005No Hit
CTTATATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCATTAAAA550.10786217175580005Illumina Single End PCR Primer 1 (98% over 58bp)
AGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGG550.10786217175580005No Hit
GCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGC550.10786217175580005No Hit
TTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGA540.10590104136024003No Hit
GAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAAGAAAAAAGATAAATTTAAACCTGA530.10393991096468004No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)