FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685
Sequences flagged as poor quality0
Sequence length66
%GC21

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69941.48367952522255No Hit
GGTTAAGGAACAGGAAGCCTGACCATCTCCCTCCACTACCTCTTCCCTGTGCTGTTACACAGTGTC60.35608308605341243No Hit
GCCCACCTCAGCTTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCTCTAGATGGTAGCTTAC50.2967359050445104No Hit
GTAACTTGCCTAACTAAGTCTCCTGGTTGACAATAGGTCTTAATGCAGTGTTTTCCGAATCCCTCG50.2967359050445104No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC50.2967359050445104No Hit
CAGAGGCTCCAAAAGGAAGAAAAAGAAAACCCAGAACAACAGAACCAAAACAACCAGTGGAACCCA40.2373887240356083No Hit
GTGCGTGAGTTCCTGGAGAAGAACTATACTGACGAAGCCATTGAAACAGATGATCTGACCATTAAG40.2373887240356083No Hit
ACTGTTTTGAGTCCAGAAGTTCGAGATTAGCCTGGGCAACATGGCAAAATCTTATCTATACAAAAA40.2373887240356083No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.2373887240356083No Hit
GAGACTATCTGCACTACATCCGCAAGTACAACCGCTTCGAGAAGCGCCACAAGAACATGTCTGTAC40.2373887240356083No Hit
ACGTCTGGCTGTGACCTCGGTGAAAGCCGACAGGAGAGAGATGGGACCCTCCTCCTCAGTAGTGGC40.2373887240356083No Hit
GGTCTCAGCTTGTATATAGAGATTGTACATTTATTTATTACTGTCCCTATCTATTAAAGTGACTTT40.2373887240356083No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAG30.17804154302670622No Hit
ACTCAGCAGGTTGAGGTGGGAGGACTACTTGAGCCTGAGAGTTCAAGGTTACAGTGAGCTATGATT30.17804154302670622No Hit
AAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17804154302670622No Hit
ACCTTCCCCTGTGACAGAAGATGAGGTTGGCAGCTGATCAGACCTCAATAATTTTATACTGTAATA30.17804154302670622No Hit
ACTTTTAAAGAAAATACCTAACAGATTTTTAATATAGTTATCTCTACCACTTTCTTTTCTAGTTTC30.17804154302670622No Hit
AGAATGGCTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCTGAGATTGCACCATTACACTCCAGCCT30.17804154302670622No Hit
GTGCTGAGATTACAGGTATGAGCCACTGTGCCCAGTTGTAGAAATTAATATAGTAATTTAAAGAAG30.17804154302670622No Hit
CCTATGACCCTGCCAAATCCCCCTCTGCGAGAAACACCCAAGAATGATCAATTAAAAAAAAAAAAT30.17804154302670622No Hit
TGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAA30.17804154302670622No Hit
GCCCAGAGGTGCAAGGAGCTGGGTATCACCGCCCTACACATCAAACTCCGGGCCACAGGAGGAAAT30.17804154302670622No Hit
GTCCTATCCTAAATAAAAAAAGACAAATTCAAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17804154302670622No Hit
GGTGGGAACGACAAACAAGGTTTCCCCATGAAGCAGGGTGTCTTGACCCATGGCCGTGTCCGCCTG30.17804154302670622No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAAC30.17804154302670622No Hit
GGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCG30.17804154302670622No Hit
GGTCTGCAAATGCATTGGCCTCCTGGTTAAGCACGTTTTGGGGATAATTTTCTCTTCTTTAGGCAA20.11869436201780414No Hit
CACATTTGGGCTCTTCGCTGGCCTTGGTGGAGCTGGTGCCAGCACCAGTGGCAGCTCTGGTGCCTG20.11869436201780414No Hit
GTGATCCTCCATCTTAAAGAAGATCAGACAGAGTACCTAGAAGAGAGGCGGGTCAAAGAAGTAGTG20.11869436201780414No Hit
CTCTGAGTCAAATCCAGTGGTGGGTAATTGTACAATAAATTTTTTTTGGTCAAATTTAAAAAAAAA20.11869436201780414No Hit
ACGCGGGGAGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGCCCTCACGGAGGCGGGGGTT20.11869436201780414No Hit
GAGCTGAGATCGTGCCATTGTGATATGAATATGCCTTATATGCTGATATGAATATGCCTTAAAATA20.11869436201780414No Hit
GAGAGCACGAGTTAGTTGTAGTCCGGCTTGCGGTGGGGCTGACTTCCTGTTGGTTTGAGCCCCTTT20.11869436201780414No Hit
GGCCCGGCGGCTGGTGGCCCGGGCCCTGGGACTCCAACACAAAAAGAAAGAGCGGCCTGCTGTCCG20.11869436201780414No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAAT20.11869436201780414No Hit
GCCCTATACTCGCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAAC20.11869436201780414No Hit
GAACATAGGTGTCTTTGTGGTTTTAAAGACAACTGTGAAATAAAATTGTTTCACCGCCTGGTAAAA20.11869436201780414No Hit
CGCCTATAATCCCAGCACTTTGGGAGGCCAAAGTGGGTGGATTGGTTTAGCCCAGGAGTTGGAGAA20.11869436201780414No Hit
GCATTCCAGCCTGAGTGACAGAACAAAACCTTGTCTCTTAAAAACAAACAAATAAACAGCCTGGAG20.11869436201780414No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11869436201780414No Hit
TGTGATCTCAGTTGTAAATAGAAAAATCTAATTCAATAAACTCTGTATCAGCCCCCAAAAAAAAAA20.11869436201780414No Hit
CAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACC20.11869436201780414No Hit
GTAATAAAAGAGATTTGACACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11869436201780414No Hit
ATGCCAATGGCAACAGCTTTGCCACTCGACTTTCCAACATTTTTGTTATTGGCAAGGGCAACAAAC20.11869436201780414No Hit
GTTACTTAGACATGCATCTTTCTATTAAATTTTATCTTAAATTTTAAAAAAAAAAAAAAAAAAAAA20.11869436201780414No Hit
GATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11869436201780414No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11869436201780414No Hit
CTTTAAGGGAGGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11869436201780414No Hit
CAACATATCTCCGGAAACCCGGCGGGCGCAAGCCGAGCGGTTGGCCATAAGAGCCCAGCTGAAACG20.11869436201780414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC20.11869436201780414No Hit
CGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAACTGGCTTTTCTGAAAAAAAAAAAAA20.11869436201780414No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11869436201780414No Hit
CTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCACGAGGTGAGAAGATCAAG20.11869436201780414No Hit
GGTATACTAGGATTTCTTGTCATAAACTATGCAAGTCAAAAGGCAGTCTTAAAAGAAAATTACAAT20.11869436201780414No Hit
GCCTCAGGTCATCCTTTTAATCTTTGCTGACGGTTCAGTCCTGCCTCTACTGTCTCTCCATAGCCC20.11869436201780414No Hit
GTGCTAGCTCCTCCCACCTCAGCACCTGCTGCATCTGGAGCAGCCCAAGCCTCAGGATGGACAAGA20.11869436201780414No Hit
GAACTTGATCGTTGGGGAACCCCAGCCCCTTGGAACTTGGAAGACCCGTGTTTCCTGGACCGCGAA20.11869436201780414No Hit
CTTTAATAAAACGTTTATAGCAGTTACACAGAATTTCAATCCTAGTATATAGTACCTAGTATTATA20.11869436201780414No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11869436201780414No Hit
GTGGTAGCACATACCTGTAATCTCAGCTACGTGGGATGCTGAGGCACAAGAATCACTTGAACCCAG20.11869436201780414No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATA20.11869436201780414No Hit
GACTTGGCAGTTATTCTATACCATTTCCTATCCATTAAAATTTTTAAAAAAAAAAAAAAAAAAAAA20.11869436201780414No Hit
GGTCGGCAAATGCATTGGCCTCCTGGTTAAGCACGTTTTGGGGATAATTTTCTCTTCTTTAGGCAA20.11869436201780414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)