FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20520
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3781.8421052631578945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1100.5360623781676412No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG460.22417153996101363No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA450.21929824561403508No Hit
GGTTATGATTCTGAAAGCAAGGAATTTAATGCAGAAGTACATCGGAAGCACATCATGGGCCAGAATGTTGC430.209551656920078No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG400.1949317738791423No Hit
GTTTAGTAGTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTT390.19005847953216373No Hit
GACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGG360.17543859649122806No Hit
CATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTTTGCAAAGGCCCTGGCCAACGTCA360.17543859649122806No Hit
GGCAATACAAATATTATCACATAGCGTCAATTTATTTGTGAATATTGAAAGCTCCAAAAAAGAAAAAAAGT350.1705653021442495No Hit
TAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAA330.16081871345029242No Hit
GATGAAATGATGGTTGCAATTTTTGAGTACATTGACAGACTTTTCAGTATTGTAAGACCAAGAAGACTTCT300.14619883040935672No Hit
CATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATA300.14619883040935672No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC290.14132553606237816No Hit
AAGTAAGGCTTCAAGGAAGTCTTGCTTAAATAAGCACTGAAGGCTTCATTTAATGCTTAAGAAGAAAATTT290.14132553606237816No Hit
CTACTGATGAGAACATTATCTGCATATGCCAAAAAATTTTAAGCAAATGAAAGCTACCAATTTAAAGTTAC280.13645224171539963No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCACTATTAAAGGTTCGTTTGTTCAA280.13645224171539963No Hit
CCAGAGAGGTGGGGCTGGATGTCTCCATCTCTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTT280.13645224171539963No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT280.13645224171539963No Hit
GACTCACTTCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACCAACCTCCACAGGC280.13645224171539963No Hit
CCTGTATAGAGCCACCATGATTCTTACAGTTGGTGGAACAGCATATGCCATATATGAGCTGGCTGTGGCTT270.13157894736842105No Hit
TGGCAAATGTGGCTACCCTGCCAAGCGCAAGAGAAAGTATAACTGGAGTGCCAAGGCTAAAAGACGAAATA260.1267056530214425No Hit
GAATCCGTGTGCGGCTGTCCAGAAAACGTAATGAGGATGAAGATTCACCAAATAAGCTATATACTTTGGTT250.12183235867446393No Hit
GTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGA250.12183235867446393No Hit
CGCGGGGGGAGCCTCATCTGCAATGTAGGGGCCGGTGGACCTGCTCCAGCAGCTGGTGCTGCACCAGCAGG250.12183235867446393No Hit
AGCATGCTGTTGGCATTGTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAAGAGAATTAATGTGCGT240.11695906432748539No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAA240.11695906432748539No Hit
CATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTCT230.11208576998050682No Hit
GGCAAAGGAGATCCTAAGAAGCCGAGAGGCAAAATGTCATCATATGCATTTTTTGTGCAAACTTGTCGGGA230.11208576998050682No Hit
GTCTATCTATCTGGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAG230.11208576998050682No Hit
TTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAA230.11208576998050682No Hit
ATCATAAGTAAAATGGGAAACTTGCCTCTGTTTTTTGCAAGGGGAGGTAAAGAGTGTTTAGTAATTACCTA220.10721247563352826No Hit
CTTTAGTGCTTTGTGCCTGCAAATTTCAAGACACCTTCATCTAAATATATTCAAGACTGCATGTCATCAAG220.10721247563352826No Hit
ATTACACACAGTGCGCTCCTTGTGCAAGCTTATCTTCCTGTCCAGTCTGCTATCGAAACTATAGAGAAGAA220.10721247563352826No Hit
GTCTGGGCTGTGTCCCTGCCGGGATTCCCAGCCTTCGGGGTCAGCCGTCGAGCCACTTGCTTTGCTCAGTT220.10721247563352826No Hit
ATTATAAGGTGGATGAGAATGGCAAAATTAGTCGCCTTCGTCGAGAGTGCCCTTCTGATGAATGTGGTGCT220.10721247563352826No Hit
GACCGAGATGAATCCTCACCATATGCTGCTATGTTGGCTGCCCAGGATGTGGCCCAGAGGTGCAAGGAGCT220.10721247563352826No Hit
GCACAAGGAAGACACACAAAACACCTGGGAAACTGAGAGAACAGCAGAATGACCTAAAGAAACCCAACAAT220.10721247563352826No Hit
GTCCGAGAGCCAGAAGAAGGAGAGGCAAAAGAGTGACAGAAGGAAGTCAAGGCACCATTATGACTCGGATG220.10721247563352826No Hit
GTACTTAGCTGTACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTT210.10233918128654972No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGG210.10233918128654972No Hit
GTATGACTGATGATCCTCCAACAACAAAACCACTTACTGCTCGTAAATTCATTTGGACGAACCATAAATTC210.10233918128654972No Hit
ATACATACATTTATCAAAAGGAGACTGTATAAGCAAATTATGGCTTAGTCATACAATGAAATACTATGCAG210.10233918128654972No Hit
GTCCTGCAGCACAAACGGCGGCGTATTGCTCTGAAGAAGCAGCGTACCAAGAAAAATAAAGAAGAGGCTGC210.10233918128654972No Hit
GCCCTGGAGGGGCTGAAAATGGTGGAAAAGGACCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAG210.10233918128654972No Hit
GAATGGTTTGGTGGCCACTGAGGTGTTGATGTGGTTTTATGTCGGAGAGATTATAGGCAAGCGGGGCATCA210.10233918128654972No Hit
GCTGAAGCCTCAGATTCCTTCTAAACACAGCTATCTAGAGAGCCACATCCTGTTGACTGAAAGTGGCATGC210.10233918128654972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)