FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002880981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002880981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16953
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2481.4628679289801214No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG530.31262903320946145No Hit
CATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTCT330.1946558131304194No Hit
CTCCTGTATAGAGCCACCATGATTCTTACAGTTGGTGGAACAGCATATGCCATATATGAGCTGGCTGTGGC300.1769598301185631No Hit
GATCAATACAAGTTCTACTCAGTGAATGTGGATTACAGCAAGCTGAAGAAGGAACGTCCAGATTTCTAAAT300.1769598301185631No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCACTATTAAAGGTTCGTTTGTTCAA280.16516250811065888No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT280.16516250811065888No Hit
CATATATGCGAATCTATAAGAAAGGTAATATTGTAGACATCAAGGGAATGGGTACTGTTCAAAAAGGAATG280.16516250811065888No Hit
TCATCACACTCTGATAATGAAAGTCTTGGTGGATTTTCTATTGAAGATGTCCAAAAGGAAATTAAAAGAGG270.15926384710670677No Hit
TAATAAGATTGAGGATCATTTCATTGATCTTCCTAGAAAAAAGATCTCTAATTTCACTAATAAGAACATGA260.15336518610275468No Hit
GATCTATACTGTTGGAAAACGCTTCAAAGAGGCAAATAACTTCCTGTGGCCCTTCAAATTGTCTTCTCCAC250.14746652509880256No Hit
CTACAGTGGCAATAATATTGAACTGGGCACAGCATGCGGAAAATACTACAGAGTGTGCACACTGGCTATCA250.14746652509880256No Hit
ACTACAGAGTGTGCACACTGGCTATCATTGATTCAGGTGACTCTGACATCATTAGAAGCATGCCAGAACAG250.14746652509880256No Hit
GAATATGGAGGCACCAAAGTAGTTCTAGATGACAAGGATTATTTCCTATTTAGAGATGGTGACATTCTTGG250.14746652509880256No Hit
GTATGATTCACAGTATAAACATTATAATCTGGAATCAACCTAAATGTTCATTAGCAGATGAATGGATAAAG240.14156786409485048No Hit
TATTTAAAGAGCATCTGTGCAGAAAACTACAAATGAATCATGAAATAAAGAAAAAATAGACTTAAATAAAG240.14156786409485048No Hit
GTAAAAGGCCCCGTGCGCGAGGGCGACGTGCTCACCCTTTTGGAGTCAGAGCGAGAAGCCCGGAGGTTGCG240.14156786409485048No Hit
GTTAGGAAGTTGTGTTTGTGTTTCAAGCAGAAAAACTGAGCTCCAAGTGAGCACATTCAGCTTTGGAAACT230.1356692030908984No Hit
TGGTCCTCCTGCCTCAGCTTCCAAAGGCACAGGCCAAGTTGTAGCTTTGTCCCTTGCCATCATGCCCAACA230.1356692030908984No Hit
GGAGAAGAGGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCC230.1356692030908984No Hit
GATTCAAAGTGGGCAAAGGCCAAGAAAGGAGAGGAAGCTTTATTTACAACCAGGGAGTCTGTGGTTGACTA230.1356692030908984No Hit
GAGCCCAGGACGTTGATGCTACAAACCCAAATTATGAAATTATGTGTATGATCAGAGACTTTAGAGGAAGT230.1356692030908984No Hit
GTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCC220.12977054208694627No Hit
GTACCAGCGTGTGTACCAGTCCCTCTGCCCCACATCCTGGGTCACAGACTGGGGTGAGCAACGGGCTGAAG220.12977054208694627No Hit
GGTTATAGCTGCCATAAGCATTATGAAGAATGGCACTACAATAAGTGCGATACAATGGTATAAATTAAATT220.12977054208694627No Hit
TGAGAATTCTTTGCCACTTTTCCCACCCCAAAACAGCATGGGGCCTGACATCTTCTGCCCTGGTCCCCTTT220.12977054208694627No Hit
CTATGGTGTATACCTAGAGGACTCAGGCCACACTCTTAGAGGTCTCTTCATTATTGATGACAAAGGAATCC220.12977054208694627No Hit
CTGTAAATCCTCAGGGAACAGATGATCTGGACAATGTAGCTGATTCAAAGGGACGGAAGCCTCTTAGCACT220.12977054208694627No Hit
CCCAAGGACAGCAATTTTGCTGGTGACCTGGTCCGGAACTTGGAAGGAGCCAATCAACACGTTTCTAAGGA220.12977054208694627No Hit
GGTTCCCACCTTACTACATGCGGAGACCCTATGGGCGTCGACCACAGTATTCCAACCCTCCTGTGCAGGGA220.12977054208694627No Hit
GTGCAGGAGCTCCAGAAGCGCTTGGACAGGCTGGAGGAGACAGTCCAGGCCAAGTAGAGCCCCGCAGGGCC220.12977054208694627No Hit
CTTCATGAGAGATTTGCAGTGTTAGCCAAATAACATAAAAATGAAAGTAGCATATATCTCTTAGAATGGCT210.12387188108299416No Hit
ACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCAT210.12387188108299416No Hit
GCAAATTGAGCACATCATTGAAAACATTGTTGCTGTCACCAAAGGACTTTCAGAAAAATTGCCAGAGAAGT210.12387188108299416No Hit
ACTTAACTTACAGGAAGATAAAGAGGATGAGAGCATCAGAAAAACAGAAATCATAGATTTCTCAACAGATG210.12387188108299416No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG210.12387188108299416No Hit
GAAACACACAGTCACATTCGAATTCGGGTGGCATCCTCCGGAGAGAGAGAGAGGAAGGAGATTCCACACAG210.12387188108299416No Hit
ACACAACACCAGGACTTAAGACAAATGGGACTGATGTCATCTTGAGCTCTTCATTTATTTTGACTGTGATT210.12387188108299416No Hit
CCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTC210.12387188108299416No Hit
GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGGCC210.12387188108299416No Hit
AGAATGTGCAGTGTCTTGTAACAACTAATTATAATGCAAATTAGGGCTACATTGTAATCTGCTTTGTTAAT200.11797322007904205No Hit
AAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGTATGAAGA200.11797322007904205No Hit
GTCAAGGCCTTCTTGGCTGATCCATCTGCCTTTGTGGCTGCTGCCCCTGTGGCTGCTGCCACCACAGCTGC200.11797322007904205No Hit
AAGAACACACAGAGCCAAAAAGCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGATGCTCACAATAAA200.11797322007904205No Hit
GATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAA200.11797322007904205No Hit
TCAATCTACTTCTCCCGCCGCCGGGAAAAAAGGCGGGAGAAGCCCCGGCAGGTTTGAAGCTGCTTCTTCGA200.11797322007904205No Hit
GATTCAATGTGTACTTACCAGTTTACTGTGTGGTTTTATCTTCAGGTACAGATAGTTTGTGGTACTACTTG200.11797322007904205No Hit
CCTTTTGGCATTTCATCTTCTAAAATGCTCTGGATTTTTAAGATAACACTTTAAAATGTGCCTTTTAAAAT200.11797322007904205No Hit
GTATATGTATTTTTTTAAGTGACCTCCTCTCCTTCCACAGACCCCACATGCCCAAAGGCCTCGGGACTTCC200.11797322007904205No Hit
GTCTTAAATAAAGAAGAGTAAACTTATTTTTTATAGAGGGTGAAGGATGCTGGAAGGGTAAGGATTTAGGA190.11207455907508997No Hit
ACGCGGGCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA190.11207455907508997No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA190.11207455907508997No Hit
GTTCTATACCTTTTAATGAATTGACTTTCATAAATTGGTTATGTTGGTGGGCAAGTTCTTTAAGCTGGAAA190.11207455907508997No Hit
GTGCATGACAGCGGCAATCTTTTCTTTGGTCAAAGTTTTCTGTTTATTTTGCTTGTCATATTCGATGTACT190.11207455907508997No Hit
CCTATTTTCTGCTTGCGTTTTTGAAACAGGAGGTGCACGTACCACCCAATTATCTATGGCAGCATGCATGT190.11207455907508997No Hit
ATGCTATGTTGGCTAAAACTGGTGTCCATCACTACAGTGGCAATAATATTGAACTGGGCACAGCATGCGGA190.11207455907508997No Hit
TAGTAGGCCTAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAAAAAAAAA190.11207455907508997No Hit
AGTTCGAACAGCACACTATCAGAGCTAAACAGATACTAGCTACTGTGAAAAACATAATGGATTCAGTAAAC190.11207455907508997No Hit
CTACTATTGATTCTAGTTCCAGTTGGTGGACCAACTGGGTGATCCCTGCCATCTCTGCAGTGGCCGTCGCC190.11207455907508997No Hit
GTGGCCCAGAGGTGCAAGGAGCTGGGTATCACCGCCCTACACATCAAACTCCGGGCCACAGGAGGAAATAG190.11207455907508997No Hit
CTTCGGGAGAACCTGGAGGAGGAGGCCATCATCATGAAGGACGTGCCCGACTGGAAGGTGGGGGAGTCTGT190.11207455907508997No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCT190.11207455907508997No Hit
ATAAGAAAGGTGATATTGTAGACATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTAC190.11207455907508997No Hit
ACTTTTAATAACAGTCTCTCTACATGACTTAAGGAACTTATCTATGGATATTAGTAACATTTTTCTACCAT180.10617589807113785No Hit
GACCATTTTCGCCTACTTTACGGGTTCTAAGGACGCCGGGGGGAAAAGCTGGTGCCCCGACTGCGTGCAGG180.10617589807113785No Hit
CTCTAGACTTTTTCTTAATTTTTAGAAATGTAAGTGGACTGAACAGAGGAAAACCAAAACACAGCTGGTCA180.10617589807113785No Hit
GGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTT180.10617589807113785No Hit
GTACCTGGGTTCAACTAAAGCGCCAGTCTGCTCCACCCAGAGAAGCACACTTTGTGAGAACCAATGGGAAG180.10617589807113785No Hit
GTTCGAAAGCATCTTGAGAGGAACAGAAAGGATAAGGATGCTAAATTCCGTCTGATTCTAATAGAGAGCCG180.10617589807113785No Hit
TCCCTACACGACGCGGGGGGAAGCATCTTGATCAACACAAGAACCCCAGCTGGGAGGTGGTGGTGTAGGAA180.10617589807113785No Hit
AGTCCTGAGACCTGAAGATTGTAATATTCATGTCTGTGAAGCTTTTAAACATTACACTTGAGATCAGTCAT180.10617589807113785No Hit
ATACATTGATCAGGAAGAACTAAACAAGACCAAGCCTATTTGGACCAGAAACCCTGATGACATCACCCAAG180.10617589807113785No Hit
ACATGATCTGCAAGAGTATTTACATTCTTTATTGAATTTGATTATATGAGACATTTTAAATTTTCATTTGT180.10617589807113785No Hit
CCCCTGCTGGCCGCAAAGTGGGTCTCATTGCTGCCCGCCGGACTGGACGTCTCCGGGGAACCAAGACTGTG180.10617589807113785No Hit
GCGTGACAAACCTTCCGTTGAGCCTGTGGAAGAATATGATTATGAAGATCTGAAAGAATCTTCCAATTCTG180.10617589807113785No Hit
GTGGATATCCCCTTGGACAAAACTGTCGTCAATAAGGATGTTTTCAGAGATCCTGCTCTTAAACGCAAGGC180.10617589807113785No Hit
GTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCCGATCACCTCTGAGACCCACCT180.10617589807113785No Hit
GTGCTTACATTTGTTAACAGATTGTAAACCACATTTATTTACATTGATGAATGGGCTGTTGAATAATCATT180.10617589807113785No Hit
CAATGACAGGGGAGAAAGTCAAGACAGTGGTTCAGTTGGAAGGTGACAATAAACTGGTGACAACTTTCAAA170.10027723706718575No Hit
GTCTTCAAGTTCAAAATTAGTATAATTCCAAAATGTCTTTTGTCCTCCAAATCAGCCAATACATTTTAAGA170.10027723706718575No Hit
GACTGGGTACCTTACATCAATGGCAAGTTTAAGAAGGATAATTAATTACACAAACCCTTCACAGACTGCTC170.10027723706718575No Hit
CATTTGTAGAAGGTGGAGATGCTGGCAACAGGGAGGACCAGATCAACAGGCTTATTAGAAGAATGAACTAA170.10027723706718575No Hit
GGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAA170.10027723706718575No Hit
ATTTATCCTCTCCATGATGTCTTCGTTAGAAAAGTAAAAATGCTGAAGAAGCCCAAGTTTGAATTGGGAAA170.10027723706718575No Hit
GACAGGGGAGAAAGTCAAGACAGTGGTTCAGTTGGAAGGTGACAATAAACTGGTGACAACTTTCAAAAACA170.10027723706718575No Hit
CTGTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGCAGGGCCTATGGTGGTTCC170.10027723706718575No Hit
GGTGTGCCTGATGTGGTAATAATGATGGTGATGATTAAGAAATTTTATGTAATATGAACCCATAGTGGCTT170.10027723706718575No Hit
GGGGTACCAAAGTGATCCTCCATCTTAAAGAAGATCAGACAGAGTACCTAGAAGAGAGGCGGGTCAAAGAA170.10027723706718575No Hit
CAAGTCTTGTGCTCATGACTGGGTCTATGAATAAGAGGTGGACACAACAGCAGTCTCCTTCAGCACGGCGT170.10027723706718575No Hit
AGACAGGGTTTTACCACGTTGGTCAGGCTGGTCTCGAACTCCTGACCTCGTGATCCGCCTGCCTCAGCCTC170.10027723706718575No Hit
ATGCTCAAGTTAGTAGAACAGATCAATATATCCCAAGACTGGTCAGACTTTGCTCTTTGGTGGGAACAGAA170.10027723706718575No Hit
GAGTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTTCAGTCATGGCATTCGCAGTGC170.10027723706718575No Hit
GGGTGGACTGCGGCTACCCCCATGTCACCCCCAAGGAGTGCAACAACCGGGGCTGCTGCTTTGACTCCAGG170.10027723706718575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)