FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6120
Sequences flagged as poor quality0
Sequence length71
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180329.46078431372549No Hit
GCCTACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA570.9313725490196079Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320.522875816993464No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG260.42483660130718953No Hit
AGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACT160.261437908496732No Hit
GCCACATGGGTATTTTATTATAGTTTTAATTTGCATGTCTCTGAACCTAAGTGAGATTGAGTATTTTTTCA160.261437908496732No Hit
GCCCGCTCGGGTATGAAGATCGGGCGGATTGAGGATGTCACCCCCATCCCCTCTGACAGCACTCGCAGGAA160.261437908496732No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA150.24509803921568626No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.24509803921568626No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC150.24509803921568626No Hit
GCCTACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTTAAAAA130.21241830065359477Illumina Single End PCR Primer 1 (100% over 58bp)
GTTTTTAGGAAAGTAAGCTTTAAGCTGCTTTTACCAGTTGAACTCCAAAAATAATGTAGGAGAGAGATGGC120.19607843137254902No Hit
GAGAAAGCCAAGATCCACTACCGGAAGAAGAAACAGCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGT120.19607843137254902No Hit
CTCCTGGATGTTTTGCTCTGTTTTGATACCTGTAATATAGGGAAACACAACTTTTTTTGGGAAAGCCCTTT120.19607843137254902No Hit
GGTTGAAGCCAAGGAAGAGTCGGAGGAGTCGGACGAGGATATGGGATTTGGTCTCTTTGACTAATCACCAA120.19607843137254902No Hit
CTCTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCAGAAGAGGAGGTTG110.17973856209150327No Hit
GTGAGTGTAAATTAGTGCGATGAGTAGGGGAAGGGAGCCTACTAGGGTGTAGAATAGGAAGTATGTGCCTG110.17973856209150327No Hit
GGTGGGGCCTCTGGGCTGTTTGCTGTGGAGCAAGGCTAATTCCTGAGCCCTTGGGGACGACAGCTCCAGGA100.16339869281045752No Hit
CAAAAAGAGAGATTATGCTAACCCCAGTAGACTGTAAGCTCCTTGAGGGCAAGGATTGTGTTGGAATTTTT100.16339869281045752No Hit
CCTTTGAATTGTGAAGGGTAATAACAAGATGAATACCTGTGGCTGCTTCACTAAAGTTGAAAAACAAGAGC100.16339869281045752No Hit
ATTTGGGCAAGAGATGCGCTTATGTATATAAAGCAAAGAACAACACAGTCACTCCTGGCGGCAAACCAAAC100.16339869281045752No Hit
ACGCGGGGGAAGCTGAAGAAAAAGCGCCAGTGAAGAAATCTATACGAGATACTCCAGCCAAAAATGCACAA100.16339869281045752No Hit
GGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAAC100.16339869281045752No Hit
GACGATGAGCTTGTGTATAACATTCACCTGGCTGTCAACTTCTTGGTGTCATTGCTCAAGAAAAACTGGCA100.16339869281045752No Hit
GATAATAGTGGATCTCAACCAAAACAGAAGTCAGATACTGTGCTTTTTCCAGCAAAGGATCTCAAGGAAAA100.16339869281045752No Hit
GTTAAAATCTCTAGGAGTGACCACGAAGTTTCATAGTTTTCCAAATGTTTACCATGAGCTAAGCAAAACTG90.14705882352941177No Hit
CACCCCGGGAAGGCGACAGTGCCTAAGACAGAAATTCGGGAAAAACTAGCCAAAATGTACAAGACCACACC90.14705882352941177No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA90.14705882352941177No Hit
GGAAAAGTAACTCGGGCCCATGGAAACAGTGGCATGGTTCGTGCCAAATTCCGAAGCAATCTTCCTGCTAA90.14705882352941177No Hit
GTACTCGTACACTATTTTTTTGTACTGCTGGTCCTGTACCAGAAACATTTTCTTTTATTGTTATTTGCTTT90.14705882352941177No Hit
GATAGAAAATGAAAATATTGTCAAGAGTTTCAGATAGAAAATGAAAAACAAGCTAAGACAAGTATTGGAGA90.14705882352941177No Hit
GTGTAGAGAAGAACATAAATGACCTGATGGAACTGAAAAACACAGCACGAGAACTCTGTGAAGCATACATA90.14705882352941177No Hit
GGAGAAAAATGAAAGGCCTTTAGATTGAAAAGAAATGAGTAAAACTGTCTATTCACAGATATTATCATCTA90.14705882352941177No Hit
GTCTGCCCATTGATAGAGGCCAGATTGTCTTGGAAGTTCCAAAGTTGCAACGATTTCTGGCTAGTGCCACG90.14705882352941177No Hit
GTGTCTAACTTCATGGCTATGGCCTTTTGGAGTCTCATCTGACATAATGAAAAGTAATCACTTGAAGAGAA90.14705882352941177No Hit
GTGTTGCTGCCTGAGTGCCGGAGACGGTCCTGCTGCTGCCGCAGTCCTGCCAGCTGTCCGACGATGTCGTC90.14705882352941177No Hit
TAATGAAGCTGAGGTTTTATATAACCGATCCTTAAACGAATATAAAGCATTAAAGAAAGATGATGAGCAGC90.14705882352941177No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCTCTCCCTACAGCCAATAGGCAG90.14705882352941177No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC80.130718954248366No Hit
GGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACAC80.130718954248366No Hit
TTTCCCTACACGACGCGGGTGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.130718954248366No Hit
TGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAAAATGGCGTGAACCCGGGAGGTGGAGCTTGCAG80.130718954248366No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTAT80.130718954248366No Hit
CTATATCACTTAGACAACTGTGTTTCATTTGCTGTAATCAGTTTTTAAAAGTCAGATGGAAAGAGCAACTG80.130718954248366No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG80.130718954248366No Hit
CCTTTCTCCGACCTTCTGGTGTGGCCGCCCTGGGGGCCAGGCCCTCGCCATCCTCCCCATCCTTGTTCCTC80.130718954248366No Hit
CCTGCCCTCCCCTCTCTGCACCGTACTGTGGAAAAGAAACACGCACTTAGTCTCTAAAGAGTTTATTTTAA80.130718954248366No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCAGAAGAGGAGGTTGCC80.130718954248366No Hit
GGTTACCAGCGTTTGAATTTAATAATGTATATTCTTTCATGCTTTTTTCTGTGCACTTACCTAAGTGTGAA70.11437908496732027No Hit
GGATGAGCTGACTCCCTTAGAGCAAAGGAGAGACGGCCCCCATTACCAAATACCATTTTTGCCTGGGGCTT70.11437908496732027No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.11437908496732027No Hit
GTGTGATGTAATTTGATTGATTCTACCACCCCTCCAGAAATATGAACGTATAGTCAGATTCTCAAAACTGT70.11437908496732027No Hit
GAGCTTGCCTCCTTTGAATGACCTAGAGCACAGGGAGGAACTTGTCCATTAGTTTGGAATTGTGTTCTTCG70.11437908496732027No Hit
GAGCACTGTATGGTATCCTTTTGCTTTATTCACCAGCATTTTGGGGGAACATTTCTTTTACATTTTAAATA70.11437908496732027No Hit
TCATTGGAGCCACTGCCAACTTCAGCCGCTTCACCCTGTGGGGTCTGGAGACCATCCCTGGCCCGGATGCC70.11437908496732027No Hit
GCCCCAGAGCCTGGGAAGGAGGCCGCTATGCAGGGTAGCACTGGGAACAGGAGACCCACCTGAGGCTCAGC70.11437908496732027No Hit
CTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTT70.11437908496732027No Hit
CACCAAACAAAAATGCCATTTTAAAAAAGGTGTATTTTTTCTTTTAGAATGTAAGCTCCTCAAGAGCAGGG70.11437908496732027No Hit
AAATGAATGAAATGAAGCGAGAAGAGAAGTTTAGAGAAAAAAGAATAAAAAGAAACAAACAAAGCCTCCAA70.11437908496732027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)