FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7459
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216729.052151762970908No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG320.4290119318943558No Hit
AATTAAGGTTGATGACAACAAGAAACTAGGAGAATGGGTAGGCCTTTGTAAAATTGACAGAGAGGGGAAAC200.2681324574339724No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC180.24131921169057513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180.24131921169057513No Hit
GGTTTAATACAGAAAAAATTGACAGGTATTATAAATTGTGGATCCAGTTACTTGCTTATTTAATTTGTAAA180.24131921169057513No Hit
GGACCCTTCAGCCACTACAACAGAATTTTTTAAATTGACAGTTGCAGAATTGTGGAGTGTTTTTACATTGA170.2279125888188765No Hit
GTTGAGAAGGATACAGTTTGTTTTTAGATGTCCTTTGTCCAATGTGAACATTTATTCATATTGTTTTGATT170.2279125888188765No Hit
TGTATGTCTTCTGGTGGGAGACTGTGAGGATCCCAGGATTCAGTATTCCTGGCCCAGAGGGCCCTTGCTGG150.2010993430754793No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA140.18769272020378067No Hit
CTTTAGAATACTCCAAGAGTTAGGGCAGCAGAGTGGAGCGATTTAGAAAGAACATTTTAAAACAATCAGTT130.17428609733208206No Hit
CCATTAATGAATTAGTCTAATCATGTTTTCTGAAAATATAACCAGCCATTGGCTATTTAAAACTTGTAATT130.17428609733208206No Hit
CCCCCGCTGTGGAGCCCATTACAAGCTGGTGCCCCAGCAGCTGGCACACTGAGCACCTGCACTAAATTACT130.17428609733208206No Hit
GAAATAAGGAGTCCAGAGACTACGCTTAACTTTCTATGCATGCATTTGAAAGCTGTTTTTACCTGATAATG130.17428609733208206No Hit
GAATTAGTCTAATCATGTTTTCTGAAAATATAACCAGCCATTGGCTATTTAAAACTTGTAATTTTTTTAAT130.17428609733208206No Hit
GAAATTAATATATTGAAAACATGTTTGAACCCTTATTTTAATTGCACCATTAAAACATTTGACTTAAAAAA130.17428609733208206No Hit
GTCCGTGCTCCTCCAGCTGCCCCAGTGAGGGCCCACCCTGCCTGCACCTCCGCGGGCTGACTGGGCCACAG120.16087947446038342No Hit
CACAACTACGGGATTGGCGCCGCCGCCTGCGTGTACTTTGGCATAGCGGCCTCCCTGGTCAAGATGGGCCG120.16087947446038342No Hit
ATTTGAAGATGACGAGGGTGCTGTGGAAATCTCAGACTGTGCTTCTTGTGCAAGTAATCAGTCTTTGACAA120.16087947446038342No Hit
GATCACGCCGTTGCAGTCCAACCTGAGCGTCCGAGCGAGACTCTATCTCAGAAAATAAAGACAGAATGAAA110.1474728515886848No Hit
CGAGCCTTAATGCTTCAAGGTGTAGACCTTTTAGCCGATGCTGTGGCCGTTACAATGGGGCCAAAGGGAAG110.1474728515886848No Hit
CTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAAAC110.1474728515886848No Hit
CCCTTATTTTGTGCCAGACACTGAGCTAGATACTAGAAATATAATAATGAACAAGCAAATATGGCAATAAC110.1474728515886848No Hit
GTTTTTAAACAAACTATAGAACTCTTCATTGTCAGCAAAGCAAAGAGTCACTGCATCAATGAAAGTTCAAG110.1474728515886848No Hit
TTCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGG110.1474728515886848No Hit
CCCTTTTGTGCTTCCTTCCCCTCAGGTAGCCTCTCTCCCCCTGGGCCACTCCCGGGGGTGAGGGGGTTACC100.1340662287169862No Hit
CTACTAAAAGTACAAAAATTAGCTGGGCATGATGGCACGCACCTGCAGTCCCAGCTACTTGGGAGGCTGAG100.1340662287169862No Hit
AGTTTCAACTACTTGGGAGGCTAAGGTGGGAGGATTACTTGAGCCCAAGAGTTCAGGGTTGCAGTGAGCTA100.1340662287169862No Hit
AGTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACC90.12065960584528757No Hit
CTTAAAGAATAAACTATGTTCTAACTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.12065960584528757No Hit
CCCCTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAAGCACGAGGAGAACCTGAA90.12065960584528757No Hit
GTATATTGTGTTTGTGTATTTACGCTTTGATTCATAGTAACTTCTTATGGAATTGATTTGCATTGAACACA90.12065960584528757No Hit
GAATCAAAAGCAGCCCTGTTTTCCAAATACCTAAAAACGACGACATTCCTGAGCAAGATAGTCTTGGACTT90.12065960584528757No Hit
GGGGACCTGCCCCCTCCTCAGGCATCTGGGAGGGCCCTGCCCCCATGGGCTTTACCCTTCCCTGCGGGCTC90.12065960584528757No Hit
AATCATGTTTTCTGAAAATATAACCAGCCATTGGCTATTTAAAACTTGTAATTTTTTTAATTTACAAAAAT90.12065960584528757No Hit
GGGTTGATGCTCATCATTTATTGAGGGGAAGCCACTTAATAAGGAGTCAGACCTAAAAGGGGGTGGGGGAC90.12065960584528757No Hit
AGATAGCCCTGTCCTGGTGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTAAATT80.10725298297358896No Hit
GAGTGGGGGTGCTGGCAAGTGTGGCAGCTGCCTCTGGGCTGAGCACAGCTTGACCCCTCTAGCCCCTGTAA80.10725298297358896No Hit
AGCATACGTTCTAGAAGACAAGGACCCTTTTTAATGTCAAAGTATTGTAAAGACCCCAAGTGATATGTGAT80.10725298297358896No Hit
CTTTGAAAACCTGTTAACCGTAGCCTCTGGACACTGAGACTGGAAGGAGAATAAAGGATGCTTGTTGTTTT80.10725298297358896No Hit
GGATATCTGATGTTTTCAAATACAAGAAATGTACAATTTATCCTTTGAGAGAGAATCATGAAGAAAAGAAG80.10725298297358896No Hit
CGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGAGGCGAGGGTTCGAGG80.10725298297358896No Hit
TTCGTAGGATGGGTGAGTCAGATGATTCCATTCTCCGATTGGCCAAGGCCGATGGCATCGTCTCAAAGAAC80.10725298297358896No Hit
GCTAGGAGGAAGTTTGGCAGATTCCTATTTAGATGAAGGCTTCCTGTTGGATAAAAAAATTGGAGTAAATC80.10725298297358896No Hit
GGCGCGGGGCGATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTCCAGCTGGTC80.10725298297358896No Hit
GTACTTACCCTTGGCTAAGAACTGCACTGCTTTGTTTAAAGGACTGCAGACCAAGGAGCGAGCTTTCTCTC80.10725298297358896No Hit
CACTTAGATCTGTTTGTTTGATAGGAGATAAAGAACAAAGAGCTGCTTTTGTCAGAGACGTTTTATTACCG80.10725298297358896No Hit
ATGTAATAATGTACAGAGGAAGCTGTTGGTGTTCTAAGACTCTGTGTGGCTGTGCAATTTCTGTACATTTG80.10725298297358896No Hit
CCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGGAGGATAGCTTGAGCCCAGGAGTTCGAGACCTGCC80.10725298297358896No Hit
CTAACGGGAATGCTAATGAGGAAAATGGGGAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAA80.10725298297358896No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA80.10725298297358896No Hit
ACAGCCAACTGATTTTTGACAAAAGTGCCTAGAATACTCACTGAGGAGTTCCTTCAATAAAGGATGCTACT80.10725298297358896No Hit
TACCGCTTCCTTAGAACTTCTACAGAAGCCAAGCTCCCTGGAGCCCTGTTGGCAGCTCTAGCTTTGCAGTC80.10725298297358896No Hit
GTATACATATATATATATTCTACAGTAAAACTGTAGACTGTCCTCGTCCTTGGCATTTTCACTGTTCTGTA80.10725298297358896No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10725298297358896No Hit
GGTTTATCCTGAAGAGTTAAAACCAGTACTAACACAAGCCAAACAAAAATTTCTGCTCCTGTTGCCTGGTT80.10725298297358896No Hit
GTTTAAAAAGACTAAAACGTTCTTTCAAAAGAGGCATCTAAATGTGTTCCTAATTTTGTATATGGGCTTAG80.10725298297358896No Hit
CCAGTTGCTGGTGAAACTGGGCCAATCTGGGGAGGGGAACATCCTTGCCAGGGAGTTTCTGAGGGTCTGCT80.10725298297358896No Hit
CTGTAAATGCTCAATAAATATTGATGATGGGAGGCAGTGAGTCTTGATGATAAGGGTGAGAAATTGAAATC80.10725298297358896No Hit
AGTATGACCGGACCCTGCTGGTAGCTGACCCTCGTCGCTGCGAGTCCAAAAAGTTTGGAGGCCCTGGTGCC80.10725298297358896No Hit
TCCCTACACGACGCGGGGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10725298297358896No Hit
GCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAGCAGCACTAAAAGCAC80.10725298297358896No Hit
TGTCTCTGGTTCTATAGTGCAAATGCTTTTATTAGCCAATAGGAATTTTAAAATAACATGGAACTTACACA80.10725298297358896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)