FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022
Sequences flagged as poor quality0
Sequence length71
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113328.170064644455493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360.8950770760815515No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG310.7707608155146694No Hit
GACCAAGATCATCGAGATGATGCCAGAATTCCAGAAAAGTTCAGTTCGAATCAAGAACCCTACAAGAGTAG210.522128294380905No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA150.37294878170064644No Hit
AATACTCATGTCTCAAGTAAGCTTAAACTACAACTTGTCACATAAAGGAAGTCTTAAGTGGAGTTCACAGA140.34808552958727No Hit
GACTACCTGTATGCTGTGAGGGACCGTGAAATGTTTGGATATATGAAATTACATCCAGAGGATTTTCCTGA130.32322227747389354No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT120.29835902536051717No Hit
CTTTCTCATCTACGTGGCCCTCCTGCGAGTCACTCCATTTATCTTAAAGAAATTGGACAGCATATGAAGAC120.29835902536051717No Hit
TTTAAGGAATCCTGATCCTCAGAACTTCTCTGGGACAATTTCAGTTCTAATAATGTCCTTAAATTTTATTT120.29835902536051717No Hit
GGGGGTAAGCTAAATCATAGTTTCTGACAATAACTGGGAAGGTTTTTTCTTAAAATAACAATGGAATTGGT110.27349577324714075No Hit
CCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACCAACCTCCACAGGCTGGGTGGCC110.27349577324714075No Hit
CCTAATTAAGGTTGATGACAACAAGAAACTAGGAGAATGGGTAGGCCTTTGTAAAATTGACAGAGAGGGGA110.27349577324714075No Hit
CCTTTTAAAATGTAAACATTTTCAGAAAAATGAGGATTGCCTTCCTTGTATGCGCTTTTTACCTTGACTAC100.24863252113376427No Hit
GTCTTTATATGTGATAGTCTTGAGTGGTTTCTTAATTATTTTCCATTCTGTTCGAACCAAGATCATATCTG100.24863252113376427No Hit
AAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.24863252113376427No Hit
ACTTGGGAGGCCGAGGCAGGAGAATCGCTTGAGCCTGGGAGGTGGAGGTTGCAGTGAGCCGAGATCGCACC90.22376926902038788No Hit
GGCAGATCACAAGGTCAGGAGATTGAGACCATCCTGGCTAACATGGGGAAACCCCATCTCTACTAAAAATA90.22376926902038788No Hit
GCCTGACTAGACAATGATGTTACTAAATGCTTCAAAGCTGAAGAATCTAAATGTCGTCTTTGACTCAAGTA90.22376926902038788No Hit
GGCCAACATGGCAAAACGCTGTCTTTACTAATAATACAAAAATTAGTCAGGCATGGTGGTGCACACCTGTA90.22376926902038788No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.22376926902038788No Hit
GACTTGATAAGAGGCGCAAAAGAAAAGAATCTCAAAGTGAAAGGACCAGTTCGAATGCCTACCAAGACTTT90.22376926902038788No Hit
CAAGCGAGTTTTTAAACAAAATGACTGAAGCACAGGAAGATGGCCAGTCAACTTCTGAATTGATTGGCCAG90.22376926902038788No Hit
GTGTAAAAGCAGTCCTACTCTTCCACACTAGGAAGGCTTTACTTTTTTTAACTGGTGCAGTGGGAAAATAG80.19890601690701143No Hit
GTCACTCCTTCCCCTGTGGATTGATGAAATAACATCCTTTGAATGAGTTTGCTCTGAGCCTGTGACTCCTT80.19890601690701143No Hit
GTTTCTGGGGTCTTTAAATTTTAAGAGAGGAACTTATGATATTCTGCTCAACTATAAGGATACACACAACT80.19890601690701143No Hit
TGTCAGGCACTGGTCTAGGTTCTAGAAGCCTATAGAAAATGTGGCAAACAAGGCTCTTGGCCTCATAAAAC80.19890601690701143No Hit
GTTCTTGGTAAGATATAAAATGGAAAAAGGCTAAGTAATATGTGAATATCATATTTTTGAAAGGTAAAAAG80.19890601690701143No Hit
CTTTGGGAGGCCGAGGCAGGCGGATCATGAGGTCAGGAGATGGAGACCATCCTGGCTAACACGGTGAAACC80.19890601690701143No Hit
CCACTGATTTTGGTTTATTCCCTCCAGGGTCTAAAGAGAACAAGAGGTGCTAGCTCTTACCAAAACAGATG80.19890601690701143No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA70.174042764793635No Hit
CCTTGGCCCTGCCATAGGGCAGAGCATGTCTGGCATAGCAGCCTGACTTTTATGCCCTAATCTTGAGTTGA70.174042764793635No Hit
ATTAAACGCCTTTGCCAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.174042764793635No Hit
CTGTCATTGTGTTTCCCAGCCTCTCCACTCCCCCGCCAGAAAGGAGCCTGAGTGATTCTCTTTTCTTCTTG70.174042764793635No Hit
GTGGAATCTATCAGCATTGTTGAAATGCTTAAGACTGCTGCTGATAATTTTGTAATATAAGTTTTGAAATC70.174042764793635No Hit
CCCCAACCTTCTGGTGGGGAGAAATAAACGGTTTAGAGACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.174042764793635No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.174042764793635No Hit
GGTGCAGATCCCGGAATGCCTGAACCAACTGTTTTGAGCCTACTTTGGGGATAAAGGATTATTTGGTCTTC70.174042764793635No Hit
GTGAATAAACACCCTGGCCCATGAGGGCAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.174042764793635No Hit
GATTTTGTGGGAATGATGCTAGCTGCTGCCACCTCAAAAACTTGTGCCACAACTATAGCATATCCACATGA60.14917951268025859No Hit
GGTGGCAAAAACATTGAACTTGCTGTCATGAGGCGAGATCAATCCCTCAAGATTTTAAATCCTGAAGAAAT60.14917951268025859No Hit
CCTCTACCCCCTCTAGAGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.14917951268025859No Hit
GCTTACTATAAGAGAATCAAGGTGCTACAGCCTGGGCTACTGTTTTTCAAACTCATTATCCAGGAAAGGAG60.14917951268025859No Hit
GAATTGCAACCCAGTGAAGAAGTTACCTGGAAGTAAAGACTGGCTAGATTATCGAATGTTCACATTTTAAA60.14917951268025859No Hit
ATTAAAGGAAGCCCTTCTTGTTTGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12431626056688214No Hit
GACTTTCTGAAGCTAGAAAAATGTTGTGTCTCCCAACCACCTTTCCATCCCCAGCCCCTCTCATCCCTGGA50.12431626056688214No Hit
ATTAAATGCTAACTACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12431626056688214No Hit
GGGCCCCAGGATCCACCTAACTGGCCTGGCACCTGGTGCCCCGTGCTGAATGAATGTACAGAGCCAGACAA50.12431626056688214No Hit
ACCATATAATGTAAAAAGATCTACATACAAACAATGCAACCAACTATCCAAGTGTTATACCAACTAAAACC50.12431626056688214No Hit
GGATTATACATACTGTCCAATTTTCATTAAATTTTTGTCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12431626056688214No Hit
GTATTAGGAAATTACTTTCACAGTAAATATCAAAGAAAAAAGATTAAGGGTCTCTTTGCCATGCTTTTCAT50.12431626056688214No Hit
CCTGCCACGCAAAAGAGTTGAAGGTTGCTAATAATTTATACTGGAATCTGGCATTTTTCCAAGCCAAGAGA50.12431626056688214No Hit
TTACAAGCTGGTGCCCCAGCAGCTGGCACACTGAGCACCTGCACTAAATTACTCAAAATGTGCTGTAAAGT50.12431626056688214No Hit
AAATAAAAAGCCTCAGTGACCCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12431626056688214No Hit
AATGTGGCTCTGGCATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12431626056688214No Hit
GACACGCAGCAGCTACAACAACAGCTGAGATCACTTAATAAATGGTGCTAAACTAAAAAAAAAAAAAAAAA50.12431626056688214No Hit
ATACCTGGGACTCATGACGGTACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATAC50.12431626056688214No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA50.12431626056688214No Hit
GCTGACCAACCTTATCCGGACCATCCGTAATGCCATGAAGATTCGTGATGTCACCAAGTGCCTGGAAGAGT50.12431626056688214No Hit
GTTTATTAATGAGGTGACTTTCACCTTAGGACAACTGTTGCATGCCAAGTTTTTTGTGTGTGTGAAACACT50.12431626056688214No Hit
TTATTGTTCTGTACTGATATTTAAATAAAGAGTTCTATTTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12431626056688214No Hit
CTCCTGTCTTGCTAGACAAGGTTGCTGTTGTGCAGTGTGCCTGTCACTACTGGTCTGTACTCCTTGGATTT50.12431626056688214No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12431626056688214No Hit
GTCATTATGCAGTATGTATAAACTTATAAGAATTAAATTGATGTGATAATTTTATGTTTTTATAATTAAAT50.12431626056688214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)