FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4502
Sequences flagged as poor quality0
Sequence length71
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116825.94402487783207No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG390.8662816525988449No Hit
GGGCTTACTTTGGGGTTGAGAGGGAAATGACTACAGTGGTAAATAAGGCGGAATCAGGATGACTGTCATTT180.3998223011994669No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT170.37760995113282986No Hit
GCCCCTGTGGCTGCTGCCACCACAGCTGCTCCTGCTGCTGCTGCAGCCCCAGCTAAGGTTGAAGCCAAGGA150.33318525099955576No Hit
GAAAAGAAGAAAAGAGAAATAGCAGAAAAGGAAAAGGAAAGAATAGAACGTGAAAAGGAAGAGCTAATGGA130.28876055086628166No Hit
GTATAGCTGATAAGATTCTCTGTAGAGAAAATACTTTTAAAAAATGCAGGTTGTAGCTTTTTGATGGGCTA130.28876055086628166No Hit
GGTAAAGGCAGACCGAGATGAATCCTCACCATATGTTGCTATGTTGGCTGCCCAGGATGTGGCCCAGAGGT120.26654820079964464No Hit
GTTTTTGTAACCATATGATTGAAAAGAAGAAAGTCTATGCTTAGGCCAGTCAGTACACCCAATTTTAAAAA110.24433585073300756No Hit
GCATATGAGGATGATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAATGGGCCAGTGAATA110.24433585073300756No Hit
GAGTATCAGCAAGAGCTGGAGAGGGAGCTTTTTAAGCTCAAGCAAATGTTTGGTAATGCAGACATGAATAC100.2221235006663705No Hit
GGAGCTGATGTTGCACCTAAACATTCCAACCCTTAAAGCTAAAACAGCACAAAAAAATTTCACTTTTGAAA100.2221235006663705No Hit
AGACTCACTCACTGCACTTCTGCTCAGTTGGCTTCTGCATCGGGATCACACAGCCATCAGCAGGACTGCCC100.2221235006663705No Hit
GTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGA100.2221235006663705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.2221235006663705No Hit
ATATAAAATTGTATTTACAGCTGATGGACTCAATTTGAGCCTTCAAACTTGTAGTTATCCTATTATATTGT100.2221235006663705No Hit
GTAGTCAAGTGCATATAAGGTTTTGAATGTTACATCTTATTTTTGGATTTTTATTTTATCATTATGGAGTA100.2221235006663705No Hit
GGTGAAGGTTCTAAGACGTGGGATCGTTTCCAGATGAGAATTCACAAGCGACTCATTGACTTGCACAGTCC100.2221235006663705No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA90.19991115059973344No Hit
CATGATCGTACTTACGCACAATCTCTGTTCAGGAAAAAAAAGATCCTGATTTTCCAGCTCCCTTCCTACTT90.19991115059973344No Hit
GATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGAAAACTGAAACCGGATCCCTT90.19991115059973344No Hit
GCATTGCCTGGCCTTCCTGTCTAGTCTCTCCTGTAAGCCAAAGAAATGAACATTCCAAGGAGTTGGAAGTG90.19991115059973344No Hit
GTTAAGCTGCTATAGTAAGTTACTGGGCATTCTCAATACTTGAATATGGAACATATGCACAGGGGAAGGAA90.19991115059973344No Hit
GGTTCTACTAGCTCCCTGCCCCTCGCCCTGCAGAGAAGAGAACCATGCCAGGGGAGAAGGCACCCAGCCAT90.19991115059973344No Hit
GGTTTGTTTTTCCTGCTGTTGATTGTTACTAGTGGCATTAACTTTTAGAATTTGGGCTGGTGAGATTAATT80.1776988005330964No Hit
GCCTGGTGGAGAACAACGAACGGGTGTTTGACCACAAGCAGGGCACCTACGGGGGCTACTTCCGAGACCAG80.1776988005330964No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1776988005330964No Hit
GTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTTCAGTCATGGCATTCGCAGTGCCC80.1776988005330964No Hit
GGAAGCAGCGGGATAAGCTGATAAAGGAAGGAAAGTACACCCCTCCACCTCACCACATTGGCAAGGGGGAG80.1776988005330964No Hit
GGCATTCCCAGCTCTGGCTTAACTGGATGCCATAAGACAACCCTGGTTCCTTTGTGAACCCTTCTGTTCAA70.15548645046645934No Hit
AGGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGT70.15548645046645934No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15548645046645934No Hit
ATTTAATAGTGCTTGCTTTTCCTCTGGGCACACCATTTTGATCATTAACCAGAGTACCTCTACTCTTAGCA70.15548645046645934No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15548645046645934No Hit
GGTCATGGGCATTGTTCAAGCCTGAGTGGCAGCCGCTCTTGCTCACCTGTTGAGGATGGGCTGGCCCACTG70.15548645046645934No Hit
ACTTTATTACTGGTTATATACATTTGGCCATTATGATGTGCAAGCAATTGGAAAAAAAGTCAAGTAAATGC70.15548645046645934No Hit
GGTTAAATAATGAGAAGGGTATTGCCTCAGTAGTGGGAAGTATTTAGATTTAGACCAAGGGTGTCCTGTCT70.15548645046645934No Hit
TGCATACACAGACAGAGGCTCTGCCAAAAGAGAGAGGCAGCAGATACAGATATTAGCAAATGACTCCTCTC70.15548645046645934No Hit
CTTTTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15548645046645934No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA60.13327410039982232No Hit
GGAGCCACTCAAAGTCTCCCGAAAGGTCTAAGAAGAGCCACAAGAAGAGCCGGAGAGGGAATGAGTAATGG60.13327410039982232No Hit
GGTGGATGTTTACCGTGTGTTATATAACTTCCTGGCTCCTTCACTGAACATGCCTAGTCCAACATTTTTTC60.13327410039982232No Hit
AATACAGTGAATTTACCCTAAAATGTCCAATCTGTATTTATGTACCTTGTCAGTGTTTTGCTGTTGGTTTT60.13327410039982232No Hit
ACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTCCCCCTCAAACCTAAAAAAAAAAAAAAA60.13327410039982232No Hit
CTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13327410039982232No Hit
GATCATCGATCATAGTATATACTAGTTACTCATAAACTGTAAAACCTAGAAGAAATGGATAAATTCCTGGA60.13327410039982232No Hit
GACCATGTGTGATCAGACTGCTATCTGAATAAAATAAGATTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAA60.13327410039982232No Hit
CGTTTGGTCTGTTTTTAGATTCTTGAAGAATTCAATAGTCTTTCAAGATGTTTAATGTGTTTAAAGCTGGG60.13327410039982232No Hit
GCATTAGAGGAACTGTAAAAATCTGCCACAGAAGGAACGATGATCCATAGTCAGAAAAGTTACTGCAGCTT60.13327410039982232No Hit
CCTGATGAGTCACAGGCTGCGGTGGGCGCTGCGGTCCTGGTGGGGCCGTGCGGTGAGGGCCGCGCGCTGGG60.13327410039982232No Hit
CTTCTGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13327410039982232No Hit
CCCTCACAGAACATGCTCAGGCCGGGCATGGTGGCGGCTCACACCTATACTCCCAGCACTTTGGGAAGCCA50.11106175033318524No Hit
CCCTGGGAGTGGGTGGAGGCAGCCAGGGCTTACCTGTACACTGACTTGAGACCAGTTGAATAAAAGTGCAC50.11106175033318524No Hit
GATTTTATAGAATAGAACCTATAGCTTCTACCATGAGTTATATGAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11106175033318524No Hit
CCAGTATTACTGAATTGTGTTTTTGCGTACAATTATGCACTGCATAATAATGTTTCAGTGAGCAATGACCT50.11106175033318524No Hit
GCGACTCATTGACTTGCACAGTCCTTCTGAGATTGTTAAGCAGATTACTTCCATCAGTATTGAGCCAGGAG50.11106175033318524No Hit
GGTTCAATTCAGTGTATATAACTGAACACACTCATCCATTTGTGCTTTTGTTTTTTTTTATGGTGCTTAAA50.11106175033318524No Hit
CATTCCAGTTTTTTAAGAAGGGACAAAAGGTGGGTGAATTTTCTGGAGCCAATAAGGAAAAGCTTGAAGCC50.11106175033318524No Hit
GGCAAGGAGGGGAATAACCCTGCAGAAAATGGAGATGCCAAAACAGACCAGGCACAGAAAGCTGAAGGTGC50.11106175033318524No Hit
CTGTAACCTGCAGTACCAGTGGATCGTTCTTGATTTTGTTTTCATTAGTGTCATTTCTTTGTCATTGAGGA50.11106175033318524No Hit
GGCTGACTGGGCCACAGCCCCGATGATTCTTAACACAGCCTTCCTTCTGCTCCCACCCCAGAAATCACTGT50.11106175033318524No Hit
GCGACGCAAGATGGCAGCCACCACGGGCTCGGGAGTAAAAGTCCCTCGCAATTTCCGACTGTTGGAAGAAC50.11106175033318524No Hit
CGGAGATGTGTATAAGAGACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11106175033318524No Hit
GTCCCGGGGCATCAGGAGAAAGGCTGGGTCTTGGGACCTTGTCCTCCCCAGTTGGCCTACTGTTACACATT50.11106175033318524No Hit
ACGCTCATTAAACAGCCTCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11106175033318524No Hit
CATTAACATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11106175033318524No Hit
TTTGTAAATAAAAGGAAAAACTCCGGGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11106175033318524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)