FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4754
Sequences flagged as poor quality0
Sequence length71
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123425.95708876735381No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG460.9676062263357174No Hit
GAAAATTCTGCATTGAAAGAATGTCTAACTGCTTACTATAATGATCCAGCCTTTTATGAAGAATGCAAAAT190.39966344131257886No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA170.357593605384939No Hit
GGTTAGCATTGCACAAAGCCCAGTTTCTTTCTGTGTTTAAAAAAGATTTTGATCCCCTTGGAATATTAAGA170.357593605384939No Hit
CATATGCACCTTGTGTCTGTACACGTGTCCTGTAGTTGCGTGGACTCTGCACATGTCTTGTGCATGTGTAG160.33655868742111905No Hit
GCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTGATGCCTTGGACAGCAGCAACTCCATGCAAACCATC140.2944888514934792No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.27345393352965924No Hit
GGTTGAAAGCAGTCACATGGTTATAATTATTCCTCCTGTGGGATTCTCCTAATGAACAATATTACTTCTAC130.27345393352965924No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.2524190155658393No Hit
CTTTTAGATCATGTAGCTGAGACATAAATTTGTTGTGTACTACGTTTGGTATTTTGTCTTATTGTTTCTCT110.23138409760201936No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT110.23138409760201936No Hit
GCCACAAGCAGTCATTCTTGACGGCTCCAGTTTTTATTTGACACTTGAGTTTAGTTTTCTCTTTTATAAAC110.23138409760201936No Hit
GGTTCTGGACAGACGTAGGCTGTCAGGGAGTGTTTACTTCGCCTCCACTTCTGTTCCTCCCTGCCCTGGTG110.23138409760201936No Hit
GCTTTTGAAGGAAGTGATGGTAATAAAGTGAAAACATTTGTGCGAGGGGGTGTTGTTAATGCAGCTAAAGA100.2103491796381994No Hit
CCTCCTCCTCCTCCTATTGCTCGGGCTGTAGTGCCCTCGAAACGTCAGCGTGTATCAGGAAACACTTCACG90.18931426167437948No Hit
GCATCCGGCAATGGACTTATCGTAGTTGGGGAAACGGGTGTTCCGAATAATATCCTGGAAGTTATCAGGAC90.18931426167437948No Hit
CTATCTTAGCCCAGTCAGGGAGCTCTGCTTCCTAGAAAGGCATCTTTCGCCAGTGGATTCGCCTCAAGGTT90.18931426167437948No Hit
CAATAAAAGGAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.18931426167437948No Hit
GTATTTTTATAGCCCTTAATAAAAAATATTAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.18931426167437948No Hit
GACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAAGAGTTTACAAAGAAATA80.16827934371055953No Hit
CATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGATGGG80.16827934371055953No Hit
AGTTATATCTACATATCTGTCATCTAAATAAATGGCTTTCAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAA80.16827934371055953No Hit
GAAACCAAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCTGTCTGTGGTTCATGAGAA80.16827934371055953No Hit
CCTTTAGAAGGACTTAGACCTCACATATTAATGTTGAGAAGTTCTGCTTAATTTTAAAATGGTTTCTATAA80.16827934371055953No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1472444257467396No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA70.1472444257467396No Hit
AGGTTTTTATGTGCCTTTTTGTTTTTGTAAAAAAGGAACACCCGGAAAGACTGAAAGAATACATTTTATAT70.1472444257467396No Hit
GTGAGCTGGAAGGATGACACTGAGAGAACAAATCGATTGGTCCTCATTGGCAGAAATTTAGATAAGGATAT70.1472444257467396No Hit
GGTGTAGAAGTTAAGGACATTGAATTGGTCATGTCACAAGCAAATGTGTCGAGAGCAAAGGCAGTCCGAGC70.1472444257467396No Hit
CAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGT70.1472444257467396No Hit
TCCTACCTGTGCCTGACCCTCCGCTCCAGGTACCACACCGAGGAGAGCGGCCGGTCCCAGCCATGGCCCGC70.1472444257467396No Hit
CGTTTGACTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1472444257467396No Hit
ATATCCAGGCTTCTACAGTGGTCACCTATGTGTATCAGCTAATTGGAGATGATGTGAAAGTAGAACGAATC60.12620950778291964No Hit
GGTGGCTGAATTGTCTGAGCCCAGGAGTTCGAGACCATCCTGAGCCACACGGCAAAACACTGTCTCTACAA60.12620950778291964No Hit
AGCTTGTGTATAACATTCACCTGGCTGTCAACTTCTTGGTGTCATTGCTCAAGAAAAACTGGCAGAATGTC60.12620950778291964No Hit
TCTTTACATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12620950778291964No Hit
GTAATACCCTTCACATGGAAGATTAATGAGGGAAATCTTTATATTCTGTATAAAAACAAAAGCAAATTTAT60.12620950778291964No Hit
TCCCTACACGACGCGGGTCGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12620950778291964No Hit
CCCCCATAGAAGGATTTCATGAGCAGATCAGGACACTTAGCAAATGTAAAAATAAAATCTAACTCTCATTT60.12620950778291964No Hit
GATCTGTTTATGTAGTTTGTTTTTAAAATGTGCCAATGCCTGTACATTAACAAGATTTTTAAAAATAAAAT60.12620950778291964No Hit
ATGCCATAGAAGTTTGCAAGAAGTTTATGGAGCGCGACCCTGATGAACTAAGATTTAATGCGATTGCTCTT60.12620950778291964No Hit
CGTTCCTTGTTGCCACTTTGGGTATTCTGAGATTTTCTCTTGCATGCCCTTAGCTTTACAGCTTTTGCATT60.12620950778291964No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12620950778291964No Hit
AGCCTGGCCAATATGGTGGAATCCCCTCTCTACTAAAAATACAAAAATTAGCTGGGCCTGGTGATGTGGGC60.12620950778291964No Hit
CCTAAGGGCATAAAACAAATTAAGAATGGAACAACTGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12620950778291964No Hit
GGGAGGTGGGGGTGAGCCCCTCACAGCCTTGCCCCTCCCCAAGGCTGGCAACCTGCCTCCCATTGCCCAAG60.12620950778291964No Hit
CCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12620950778291964No Hit
CAATAAAGCTATGTCTGATATTTTCCTTCACTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12620950778291964No Hit
GGCCGATGACTGAGCCCCGAGGGCCCAACACAGATCATGGATTTGCGGCCAGCTCTCCTGGGGGTAGGGGG60.12620950778291964No Hit
GTGATGTGAATGGCACCGAAGTCAGATGAGTATCCCTGTAGGTCACCTGCAGCCTGCGTTGCCACTTGTCT60.12620950778291964No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC50.1051745898190997No Hit
GAACAGGCAGAGGTAAAAAGATGATGGAAGGTGTGGTGACTAAGGGCCACGGTTATTGGGTGAAATTTGAG50.1051745898190997No Hit
GTGAAAAGTTTGTGAGTGAAGAATGCTGAGAAGATTGTAATGCTTTGTACAAATAAACGTCTTGTTAAAAA50.1051745898190997No Hit
GGCTTTAGAGAAGTATAGGGGGCTTCTTCCCTGCTGGGCTTACCACACTGCTCTCAGGCCTCAAACCCTTT50.1051745898190997No Hit
TAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC50.1051745898190997No Hit
GCCTTAGACAAGCGCCAAGCCCATCTTTGTGTGCTTGCATCCAACTGTGATGAGCCTATGTATGTCAAGTT50.1051745898190997No Hit
ATCTTATTGTGGGGAGGGGAAAGAAATCCATCTGCTCATGAAGCACTTCTGAAAATATAGGTGATTGCCTG50.1051745898190997No Hit
GGGAAGAGGAGGAGAGGGGAGGGGACCTGCCCCCTCCTCAGGCATCTGGGAGGGCCCTGCCCCCATGGGCT50.1051745898190997No Hit
GTATATTAATGTAAAACAATGTTAATTTACTCAAGTTTTCAGTTTGTACCGCCTGGTATGTCTGTGTAAGA50.1051745898190997No Hit
GTGCAGCTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1051745898190997No Hit
AAATACCTAATGATGTGGGCCTAAATTTGCCTCATTCAGACTGAGACAGGGCTGTTAAAATTCATTTCTAA50.1051745898190997No Hit
AGTCCCACTATGCGCTGCCCCTGGGCCGCAAGAAGGGAGCCAAGCTGACTCCTGAGGAAGAAGAGATTTTA50.1051745898190997No Hit
GAATGAGACTCTGTCTCAAAAATAATTAAAAAGCCTCTGCCCCAAACTCGTTAAAAGATTTTATAACCACA50.1051745898190997No Hit
GGTTTGGGGGGCGGCCGGTGGGGGAATCCCCTGCCTTGGCCTTGCCTCCCCTTCCTGCCACGTGCCCCTAG50.1051745898190997No Hit
CCTTCTGTACTTGAAGTTGATTTGAAATATGTAAGAATTGATGATGTATTTGCAAACATTAATGTGAAATA50.1051745898190997No Hit
GGGTTTACCCTTCGAGAGACGGGGCTTCGCCATCTTTCCCAGGCTGGTCTCAAACTCCTGAGCTCAAGTGA50.1051745898190997No Hit
GTCCCAGCTGCGCGTCGCCAAAGTGACAGGCGGTGCGGCCTCCAAGCTCTCTAAGATCCGAGTCGTCCGGA50.1051745898190997No Hit
GGCTACACAATTGTTACACTTTATTCAGATTACAATTAATTAGAGTGATTATGAATTAATTAGTGTTCTAC50.1051745898190997No Hit
GCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGAGGGCGGATCACGAGGTCAGGAGATCGAAACCA50.1051745898190997No Hit
CTTTGGGGAGCAGGAGGCCAATAAAAAGTTCAGAGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1051745898190997No Hit
AAATTTAAAGCATTCCTTTCTTTAATTTTGTAATTCTTTACTGTGGAATAGCTCAGAATGTCAGTTCTGTT50.1051745898190997No Hit
CATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1051745898190997No Hit
ACTTTGCATAGACATCCTCAGAATCGCAATCAGTCATGGGGTTGCCCTTCCAAGCTGGCAGAAAAGGAACC50.1051745898190997No Hit
CGTGAAGAGCAGCACAACATGGAAAGAGCCAAAATGTCACAGTTCCTATCTGTGAGGGAATGGAGCACAGG50.1051745898190997No Hit
GTTCCACAAGGTTACTGTATACATAGCCTGAGTTTAAAAGGCTGTGCCCACTTCAAGAATGTCATTGTTAG50.1051745898190997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)