FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15805
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3972.511863334387852No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG840.5314773805757672No Hit
ACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATT500.3163555836760519No Hit
GTTCTACACCATTACTCAATTCTTAAAAATTAGAAATTGCTGTAGCAGTATTCACTATAACTTAACACTAC460.29104713698196777No Hit
CTCTAATTAATTGTAATCTGAATAAAGTGTAACAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAA410.25941157861436254No Hit
TATTAAAAAGGAAACTAATTGGACCATTTTCTATTTGTCTATTTTATACAAAAAGGCTACACAATTGTTAC410.25941157861436254No Hit
ATATTAAAAAGGAAACTAATTGGACCATTTTCTATTTGTCTATTTTATACAAAAAGGCTACACAATTGTTA370.2341031319202784No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC330.20879468522619424No Hit
GTGTAACAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCCTTTTTAA320.2024675735526732No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA300.18981335020563112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280.17715912685858906No Hit
ATGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTT280.17715912685858906No Hit
GTTACACTTTATTCAGATTACAATTAATTAGAGTGATTATGAATTAATTAGTGTTCTACACCATTACTCAA280.17715912685858906No Hit
GGTGGATCTATTTCATGTTTTTGATCAAAAACTATTTGGGATATGTATGGGTAGGGTAAATCAGTAAGAGG280.17715912685858906No Hit
CTATTTGGGATATGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTA260.16450490351154698No Hit
GGTTACAACAACTTTGGGATAAAAGGCAACTGGTAAACTGTCCAAAACAAGGTTCCAAATAACACCTCTTA260.16450490351154698No Hit
AATTAGTGTTCTACACCATTACTCAATTCTTAAAAATTAGAAATTGCTGTAGCAGTATTCACTATAACTTA260.16450490351154698No Hit
GCAAAACAGAATCCAGAATCTCTCAAATGCATCATTCAAAATCCCAAGTATCCAATCAAAAAACACTAGAC250.15817779183802594No Hit
GGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTTTTATCCCAAAG240.1518506801645049No Hit
GATTAAGATGAATTGTGAAATTTACATCAAGCAATTATCAAAGCGGGCTGGGTCCCATCAGAACGACCCAC230.14552356849098388No Hit
CCCTATCTCTACTAAAATAACTAAAATAAGCCAGGTGTGGTGGCACGCACCTGTAGTACCAGCTACTTGGG230.14552356849098388No Hit
GTTAAGGACAATGAGCTCATTACCAAGCCAGTTATTGATTCTCTGGTTCCAAAATAGAACAGTACAGGAAG220.13919645681746284No Hit
CACGTGTTCTTAACAATTATGCTTGGATTGTTCATGAAAATTTCATAAGACATTAAACAAAGCTAGCCATC210.1328693451439418No Hit
CCTATAATCCCAACACTTTGGGAGGCTGAGGTGAGCACTTGAACCCAGAAGTTGAAGACCAGCCTGTGCAA210.1328693451439418No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT210.1328693451439418No Hit
GATGAATGTGTCTTCCATTTAGCTAGAGACTGTAGCATAGCCTCTTTAGGTGTTGGTGGATCAGTAAAAAC200.12654223347042076No Hit
CCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATTAAAAAGGAAACT200.12654223347042076No Hit
GGAGAATCAGCAGAACCTGTAGGATCTTATTTGGAATTGACATTCTCTATTGTAATTTTGTTCCTGTTTAT200.12654223347042076No Hit
CTTTATTGCAGCATCGGCAAAGGTCAGATTTCTGAAGCTGGTGAAGATTGGGCAGCATTTCCATGTGAAAT200.12654223347042076No Hit
ATATTGTATATTGCCATATCGTCTGGTGAAAGGGTTAAATTACTTCACCTCTTGCACTTTTAGATGCAAAT200.12654223347042076No Hit
AAAATGGTCCAATTAGTTTCCTTTTTAATATGCTTAAAATAAGCAGGTGGATCTATTTCATGTTTTTGATC200.12654223347042076No Hit
TTGTATTACTTACTGCAAGAGATATTTCATTTTCAGCACAGTGCAAAAGTTCTTTAAAATGCATATGTCTT200.12654223347042076No Hit
TCAGTCTTCCTTACACAATAATCATGAAAACTGAACAATGAAGTTCTTTAACATGTACAAAAACCTGTCAT200.12654223347042076No Hit
GCATTTACATAGTCCATGATAGTTTACTTGTGGGATTATTTGGTTAATCTTTGCCTTTAACACCAGGGTTC190.12021512179689971No Hit
TTACAAAATATTTCCTCAGTACTTTGCAGAAAACACCAAACAAAAATGCCATTTTAAAAAAGGTGTATTTT190.12021512179689971No Hit
AAGTAAATAAGCTGAAAAACTAAAAAGCTCCTCTTTTTATCACAGTTCTTCCACATAAGGCTGATCAACAT190.12021512179689971No Hit
ACCAAAAAAGCATTCCCTATTTCCAACTGCAGTATGTATAAATACATATCACTACAAAATGTATAGAAAGT190.12021512179689971No Hit
AATCTGAATGATCCTACTCCTTTGGAGTAAAACTAGTGCTTACCAGTTTCCAATTGTATTTAGCTTCTGGT190.12021512179689971No Hit
CTATAAGGCTCATGTCATAGTTCAGCACCAAAAAGATCTACACAAAACTGTTTAACCAATCTTCTTATCTA190.12021512179689971No Hit
AGCCAAAAATCATCTATACAAGTAAAAAGTGTGTGGGTCAGGCATGGTGGCTCCCACACCTGTAATCCCAG180.11388801012337868No Hit
TATTTATACTTATTATCAAAGTGATTGCATATTGAGGCACAGAGCTTAAAGAGGAAATATATATTACTTAT180.11388801012337868No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT180.11388801012337868No Hit
ATAGAATACACTTTGAACTGTGAGGTATGGCAGCTCCCTATGAGAGAGAGCTAACATGTAAACCTGAATAT180.11388801012337868No Hit
GTTCTACATTGATGTGATTGAAGTCCCTCCGCAGGGTTCCTCTGGGGCCCTTCACGATAACTGTGCGTCCC180.11388801012337868No Hit
GTTTTGGATTCTTTTGGGTTTTTGTTTTGTTTTGAAGATCAGACAGTGATCACTCTGAAAGATTGAAGCCA180.11388801012337868No Hit
ATTTAACGTGGCAAAGGGCAACATTTAACATAATTCAACTGCTTTTACCTAAATACGCTTACTGCTTAAGT180.11388801012337868No Hit
GTCTTACATGATAATTTTCCCTGGTAACAACTTAGTAAGTGATATATCTTTTTTCCTAAATTGCTTAAATA180.11388801012337868No Hit
TTTTTGAAGTGAAATTTAACTTTTTGTGCAAGTAGTACTATTATACCCATCTTCAGTGTCTTACTTGTACT180.11388801012337868No Hit
GTAAAATGCAAACATCAATCTTTCAAAATACAAGATCAGAAGGTAGTTTTCTTCTTTTTTGTCTATTTGTG170.10756089844985764No Hit
CTATTGGATGTGATTCTAAAAGCTTTTATTGAGCATTGTCAAATTTGTAAGCTTCATAGGGATGGACATCA170.10756089844985764No Hit
ATACTGAGGAGTGGGGATGGCCAAGCCTTGGCTGTTGAGTAGGCACTGCCCAGTTGTGCTGTATGGGATGC170.10756089844985764No Hit
CATCGTATCACAGCAGGTTACAACAACTTTGGGATAAAAGGCAACTGGTAAACTGTCCAAAACAAGGTTCC170.10756089844985764No Hit
TGCTTATTTTAAGCATATTAAAAAGGAAACTAATTGGACCATTTTCTATTTGTCTATTTTATACAAAAAGG170.10756089844985764No Hit
ATCCTGAATGGTCTGTCATTGTCTGCCTTTAAAATCCTTCCTCTTTCTTCCTCCTCTATTCTCTAAATAAT170.10756089844985764No Hit
CCAATGAGTTAAGGGAAACTAGTTGTCTCAGCCAGGCTTAGTGGCTCACACCTATAATCCCAGCACTCTGG170.10756089844985764No Hit
CCTCTCCCATCCTCAAAAAAGGGCCATTTTATGATGCATTGCACACCCTCTGGGGAAATTGATCTTTAAAT170.10756089844985764No Hit
TAATTAGAGTGATTATGAATTAATTAGTGTTCTACACCATTACTCAATTCTTAAAAATTAGAAATTGCTGT170.10756089844985764No Hit
ATAAAAATCTTTAGTGACATAAGCCTTACAATCGTATACAACATTCACATGGCAATATTAGACAGTTAAGC170.10756089844985764No Hit
TAGTTTACTCAGCTTCACTGTTACATCCTAGATGAGTATTGTATTCAAAAATACTGGGCCTTAAGTCTTCA170.10756089844985764No Hit
GTATCATATGACCTTTGATTATGAAAAAATCAGAATAAAAATCTTTAGTGACATAAGCCTTACAATCGTAT160.1012337867763366No Hit
ATTGTTTCCTGTTTAATGCATGGGCTGAAATCACTAGGATCAATTTCCTTCTTGAAGAAAGAGGAGAGGAA160.1012337867763366No Hit
GTCCAAAGTCAGGTGGAGTTCCAATGTATGAAAAGCTTGAAAAATCTACCTTAAGGAGACTGAATATCAAT160.1012337867763366No Hit
AAAATAGACAAATAGAAAATGGTCCAATTAGTTTCCTTTTTAATATGCTTAAAATAAGCAGGTGGATCTAT160.1012337867763366No Hit
GGCGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCATTGTGGCGTGTGCCTGTAATCCCAGCT160.1012337867763366No Hit
TCATTGTTCAGGAACCTCAAGGTAGACAAGATAGCTCCCAGAAAATCATCCATTGGAATTTTCCCTAGGCA160.1012337867763366No Hit
GGTAAAAGGTCACCAAATGTTTATGAGAGAGGAAATAAGAATAAAAAAGACATTTAGTTCTCAGGAACAAG160.1012337867763366No Hit
GATACATCCTATGGGTATTAAAAAGCCAATAGAATATTATGAATAATTTTATGCTAATAAATTTAACAACT160.1012337867763366No Hit
CTTAAAATAAGCAGGTGGATCTATTTCATGTTTTTGATCAAAAACTATTTGGGATATGTATGGGTAGGGTA160.1012337867763366No Hit
CCTGTAATCCCAGCACTTTCGGAGGCTCAGGCGGGCAGATCATCTGAGGTCAGGAGTCCGTGACCAGCCTG160.1012337867763366No Hit
TCCGAGACCAGCCTGGCCAACATGGTGAAACCCTATCTCTACTAAAATAACTAAAATAAGCCAGGTGTGGT160.1012337867763366No Hit
GATGTTACCTGCTTGTGAGTATCAATTACTTAGTTTTTAAATTTTTAAGGTATTGCCTACTTTTCTTTAGA160.1012337867763366No Hit
CTGGTAAACTGTCCAAAACAAGGTTCCAAATAACACCTCTTACTGATTTACCCTACCCATACATATCCCAA160.1012337867763366No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT160.1012337867763366No Hit
ACCAAGTACTGTTTGCTTTCCTTTGTTTCAGATGGAGGAGATGTATAAGAAAGCTCATGCTGCTATACGAG160.1012337867763366No Hit
CCCAGAATGATATCTCATAAAACAAAGGCAATGAGGTCTCTTAAGACCTGGATTGGCCAGGCGTGGTAGCT160.1012337867763366No Hit
CAGTAACAGGTCAAGCTCTTTAGCATTCTTTGACTTTTCCCAAAATATAGATATGACATTCTCCTTTATCT160.1012337867763366No Hit
GGTAAACTGTCCAAAACAAGGTTCCAAATAACACCTCTTACTGATTTACCCTACCCATACATATCCCAAAT160.1012337867763366No Hit
GTCTTGGTGTTTGTATGAAATTCTGAGGCCTTGATTTAAATCTTTCATTGTATTGTGATTTCCTTTTAGGT160.1012337867763366No Hit
TTTTCTATTTGTCTATTTTATACAAAAAGGCTACACAATTGTTACACTTTATTCAGATTACAATTAATTAG160.1012337867763366No Hit
CCAGAAGGTCACGTGGAAATGTCTCGGGACTTGGGTCCCGGAGTGCCCGTGAAGCGTGTTTTTGCTCCTGA160.1012337867763366No Hit
CATATGGAAATACAATTATAAGACCAAGGAGACACTGAAAATCAATGGTAAAGAAAAACCTATTCAATAAA160.1012337867763366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)