FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12530
Sequences flagged as poor quality0
Sequence length71
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG5364.277733439744614No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3182.5379090183559456No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA1881.5003990422984836No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT1601.2769353551476457No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT1301.0375099760574622No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC1090.8699122106943337No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC850.6783719074221868No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCC760.6065442936951316No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC720.5746209098164405No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.5666400638467678No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.5107741420590582No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC630.5027932960893855No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA620.4948124501197127No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.4868316041500399No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT570.4549082202713488No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC540.43096568236233046No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.4229848363926576No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.3671189146049481No Hit
GTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAA380.3032721468475658No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.295291300877893No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC370.295291300877893No Hit
GAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTA360.28731045490822027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330.2633679169992019No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA330.2633679169992019No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.24740622505985635No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGC310.24740622505985635No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.24740622505985635No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA300.23942537909018355No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAG270.21548284118116523No Hit
CCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.20750199521149243No Hit
CAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTAT260.20750199521149243No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.19154030327214686No Hit
AATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAA220.17557861133280128No Hit
CGTTGAAGTCTTCAAAATGCTTGCTCCAGTTTCACTGATACCTGCTGTTTCTGAATTTGATGGAACATGTT220.17557861133280128No Hit
GCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATT210.16759776536312848No Hit
TCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGG210.16759776536312848No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCA210.16759776536312848No Hit
ATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.16759776536312848No Hit
CCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.1516360734237829No Hit
CTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAA190.1516360734237829No Hit
GGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTA180.14365522745411013No Hit
ACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.13567438148443736No Hit
ACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTA170.13567438148443736No Hit
CATACCTGCAGCTGCTTTTCTAACAAACTGTTGATCAGCAAAAATAAAGGGGCTACAGAAACACTCATTTT170.13567438148443736No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACAC170.13567438148443736No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC160.12769353551476456No Hit
CCTGGGGGCCTTGGCTTTGATCTACAATGAAGCCCTCAAGGGCTGAAAATAAATAGGGAAGATGGAGACAC160.12769353551476456No Hit
ACATGCACATACCTGAAAATGTACAACACAATGCTTGTCAATGGAGCAAGGACCAAGTTAACTTATCACCA160.12769353551476456No Hit
GTCTGTAGCTGTATATAAAGCGCATGTAGAGCTACAGATCCAGATACACACACTTGTGTATATATGTACAT160.12769353551476456No Hit
CTCTAGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.12769353551476456No Hit
GAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTA150.11971268954509177No Hit
CCAGGTGGAAGTGGGCTAACTGGAGCTAAACTTCAGGACTGTATGAGCCGAGCAGTTACAAGACACAAAGA150.11971268954509177No Hit
TCTGTGATAAGATGGCCATTTAATTTTTAAATTACACACAGTGCATGGAAAAAAAAAAAAAAAAAAAAAAA150.11971268954509177No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCT150.11971268954509177No Hit
GCAGAACAATGTGGAACACAAGGTTGAAACTTTTTCTGGTGTCTATAAGAAGCTCACGGGCAAGGATGTTA140.11173184357541899No Hit
GTTACATTTAAACTATATCCATGGATATCAGTCTGCTTTGGACTCCTCTGCTAGTGTTACAGATGGAAATA140.11173184357541899No Hit
CCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC140.11173184357541899No Hit
TTTCAAATTCAGCGGCTTTGATTCAGCAAGCCACAACAGTTAAAAACAAGGATATCAGGAAATTTTTGGAT140.11173184357541899No Hit
CCTATGTGTATCAGCTAATTGGAGATGATGTGAAAGTAGAACGAATCGAATACAAAAAACCTTAAAGCCAG130.10375099760574621No Hit
GGTCAATAGTTTCCCAATTTCAGGATATTTCGATGTCAGAAATAACGCATCTTAGGAATGACTAAACAAGA130.10375099760574621No Hit
GGAGAAGGGTCTCTCTAGCAGTCAACTCCATTTGTAATAAAGCCCTAGCACTCCAAAAAAAAAAAAAAAAA130.10375099760574621No Hit
CATCTCATATTTGGAATGACTATTAAAAAAACAACAATGTGCAATCAAAGTCCTCGGCCACATTGTGAACT130.10375099760574621No Hit
CCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.10375099760574621No Hit
GTGCCAGGTGCACAGTTCCAGGTGTCAGGAAAGCTAGCGAGTGCTGGGGCCGGGCGGTAGAGAGCGGGGCT130.10375099760574621No Hit
TACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCC130.10375099760574621No Hit
CTTTAAGGATGGTTTAGAAATGGAAAAATGTTAACAAATGTGGCAATTATTTTGGATCTATCACCTGTCAT130.10375099760574621No Hit
ACTTAGCCTTTGGATTTAGAAGTAAAAATAAAGTATCTCTGACTTTCTGTCAAAAAAAAAAAAAAAAAAAA130.10375099760574621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)