FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666
Sequences flagged as poor quality0
Sequence length71
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42025.210084033613445No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT150.900360144057623No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA140.8403361344537815No Hit
GGTCGATGAAACAGGTGTAGAAGTTAAGGACATAGAATTGGTCATGTCACAAGCAAATGTGTCGAGAGCAA130.78031212484994No Hit
ACATAACATTTTAATAGATGAAATAAATACTCCAGTACATGCAAGGTAAAAACTTGACGTTGGGGGAAAAA130.78031212484994No Hit
TAGTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGAC120.7202881152460985No Hit
GGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTT120.7202881152460985No Hit
TGAAAAAACATTCCATGCTCAATGATAGAATCAATATTGATAAATGACCATACTGCCCAAAGCACTTCACA120.7202881152460985No Hit
ACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCGT120.7202881152460985No Hit
GGGGCTGCCCCAGGCGCGCAAGCGACAGCGCCTCACGCACCTGAGCCCCGAGGAGAAGGCGCTGAGGAGGA100.6002400960384154No Hit
CCCCGTCTCTACTAAAAATACAAAAATTAGCTGGCATGGTTGCACACGCCTGTAATCCCAGCTACTCAGGA90.5402160864345739No Hit
CTGGCAACAGCTTTGCAGAAGCTGGAGGAAGCTGAGAAGGCAGCAGATGAGAGTGAGAGAGGCATGAAAGT90.5402160864345739No Hit
GTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCA90.5402160864345739No Hit
GTTTGTTTCAATTGGCCATTTAAGTTTAGTAGTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCT90.5402160864345739No Hit
ACTTTGTGAGATCCACACCTGCCCACCAGAGAACAAACCCCCTTTGACTGTAATTTTCCATTACCTTCCCT80.4801920768307323No Hit
GACCGGACGATTCAGTTTCACATTGCCATATCCAGTGAAAATCAAAGTTAGATTTTCCTTTTTTCTTCAGA80.4801920768307323No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGG80.4801920768307323No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA70.42016806722689076No Hit
GCTCCTTCTCTGGTTTGCAGTGTAATGAGTTCATAAGCTAAATTTTCAGAAAGAATTTTGTTTAAGATTAT70.42016806722689076No Hit
GCCTGTCAGGTAGCCAGGACTACAGGTACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAA70.42016806722689076No Hit
GTACCACATAACCTGGGGTGAGTAGTAGGTTATGCTATCTCTATTTACACATAGAATACACTATATGATGT70.42016806722689076No Hit
TCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAAATCAGTACTTTTTAATGGAAACAACTTGAC70.42016806722689076No Hit
GAATCACTTGAACCGGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCACTGCACTCCAGCCTAGGCAA70.42016806722689076No Hit
GGCCAAACTGCCTTAATTCTGAGTGCTGTGTGTTCTGCAGGTGACAGTTTCCTGAGGAGAGGGGTGTATCC70.42016806722689076No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGGA60.36014405762304924No Hit
GCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGTGGAGAAGAAAATTGACAAATACACAGAGGTCCTCA60.36014405762304924No Hit
ATGTTTGTCTCATTACCTTTTTGTATAGTTTATTAAAGTATTAATATAGTTTTAATAAGTAAATATTTTTA60.36014405762304924No Hit
GCTTCCCCCATAGAAGGATTTCATGAGCAGATCAGGACACTTAGCAAATGTAAAAATAAAATCTAACTCTC60.36014405762304924No Hit
GCACTCTAGCTGGGCTGACAGAGTGAGACTGTCTCAAAAAAAAAAAAAGAACACATCATAGAACTTGGGAT60.36014405762304924No Hit
GGCTGAGGTGAGCAGATCACCTGAGGTCAGGAGTACGAGACCAGCCTGGCCTACATGGTGAGACCCCGTCT60.36014405762304924No Hit
GTATTAAATAAATGACTTATTTCTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.36014405762304924No Hit
GAGGAGAAGAAAGATGAGAAGAAGGAGGAGTCTGAAGAGTCAGATGATGACATGGGATTTGGCCTTTTTGA60.36014405762304924No Hit
GCCTGTAATTCCAGTACTTTGGGAGGCCGAGGCGGGTGGATCACAAGATCAGGAGTTTGAGACCAGCCTGG60.36014405762304924No Hit
GCACTTTGAGAGGCGGAGGTGGGCGGATCATGAGGTGAGGAGATCGAGACCATCCTGGCTAACACAGTGAA60.36014405762304924No Hit
GTGCAATATCCATTAAAGAAATTGAAGCCGAAAGCAGGAGGAGCTTTAGACCAGGAGTTTGAGACCAGTCT50.3001200480192077No Hit
GGAGTTTGAGACCAGCCTGACCAACATGTTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCCCAGCG50.3001200480192077No Hit
GCTGTGAAAGGCCAGCTCTCGGCAGCCCCTGTGGTTTCAGCGCTGCCGCTCTGTGTCATTCAGGTTGTGCA50.3001200480192077No Hit
GTAGTCCGCCACAGGGCTCTGAGCTGCACTGGCCCCGGTGCTGGCATCTGGTGGAGCGGACCCACTCCCCT50.3001200480192077No Hit
CCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGTCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAA50.3001200480192077No Hit
GCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTGAAAGTTCATATCTGGAGCCTGATGTCTTAACG50.3001200480192077No Hit
GATTCAGAATCTGAAGAAGAGAGAGAGAAAAGCAGTTGTGATGAAACAGAATCTGATTATGAGCCAAAAAA50.3001200480192077No Hit
CAGTTGCGGTGGGTGTCATCAAAGCAGTGGACAAGAAGGCTGCTGGAGCTGGCAAGGTCACCAAGTCTGCC50.3001200480192077No Hit
GTGGACCACTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAAATCAGTACTTTTT50.3001200480192077No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG40.24009603841536614No Hit
AGGCAGCACCTAAGCAAAAGATTGTGAAGCCTGTGAAAGTTTCAGCTCCCCGAGTTGGTGGAAAACGCTAA40.24009603841536614No Hit
GTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAAATCAGTACTTTTTAATGGAAACAACT40.24009603841536614No Hit
GGATGGTATTAATCTTGTGTAGTCTTCAACTGGTTAGTGTGAAATAGTTCTGCCACCTCTGACGCACCACT40.24009603841536614No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.24009603841536614No Hit
GGTCTAGTGTGTTCTTAGTGGTTATACTGGGAAGTGTGTGGAGATTTAGGTGCTGCTCTGCTGCTCTGGAT40.24009603841536614No Hit
GGATCACTTGAGGCCAGGAGTTTGAGACCAGCCTGGGCAACACGGTAAAATTTTTTGTATTCTGTACAAAA40.24009603841536614No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTGGCC40.24009603841536614No Hit
TTTTAATGGAAACAACTTGACCAAAAATTTGTCACAGAATTTTGAGACCCATTAAAAAAGTTAAAAAAAAA40.24009603841536614No Hit
GCCATATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA30.18007202881152462No Hit
GGCCTGATTGATAACGTGATCCGGCGGAGGCCGATCGCGACGGCTGGTTTGCATCGCCAGCTTCTATATAT30.18007202881152462No Hit
GAGTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTTCAGTCATGGCATTCGCAGTGC30.18007202881152462No Hit
GAGCTGCTGGGGCCCAGCGACAAGAGCCAGGCAGCCCTACAGAAGGCTGGGGAGGTGGTCCCCCCCGCCAT30.18007202881152462No Hit
CGGTGGGTGTCATCAAAGCAGTGGACAAGAAGGCTGCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAA30.18007202881152462No Hit
GGGCAGGGAATCCTGGAGCACTTGTTCCGGGATGGTGTGGTGAAAGAGGGGATGAGGGAAAGAAATGGGGG30.18007202881152462No Hit
GACTCACTCCCCTCTGCAAATACCTGCATTTCTTACCCTGGTGAGAAAAGCACAAGCGGTGTAGGCTCCAA30.18007202881152462No Hit
GGCCAGGTCTCGGGGGCTCCGGGAGGTGCTGCCTGCCAGGGGAAGCTGGAACCAGCAGCAAGGAGCCCGGG30.18007202881152462No Hit
ATTTAATTGCAGTGTACAAAATTGTGTTTGTATATAGAATAAACTGTCTGTTGACAGCGAAAAAAAAAAAA30.18007202881152462No Hit
CCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACAGAATCGCCGGACAGGTGGCAG30.18007202881152462No Hit
GGGAAAAAAACTCGCACATCTGGTGTCAAAAGTGTTCTGTGTGAGGGTGTAAAGAAACAAGTGTATTTCTT30.18007202881152462No Hit
TAATAATCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18007202881152462No Hit
GATTAAACTTTGCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18007202881152462No Hit
CATACTCAGTACAGACCAGTCTGCAGCTCTTGTTGTGTATTTAGTAAACATGTGATACCAATGAACTATTT30.18007202881152462No Hit
CCAGCTCCTTGGACCCCACCTCCCATGCTCTCACTCTCATCCCCGTTCTCTTGTCCACACAGCTCTTCCAA30.18007202881152462No Hit
AAAAATAAATGTTTTGATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18007202881152462No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18007202881152462No Hit
GGGAAGGTCTCTCTCCATAACCCCCGTCCCTCCTTCTTTGGCCTCTTATCTTCTTTTCTTCCTTTCTAGAA30.18007202881152462No Hit
ACATGTTTATATACAAGTCACAAATTTACATTAAGAAGTAAATTAAGGCATTTTCCCCAATGTATAGAAAC30.18007202881152462No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18007202881152462No Hit
GGCCATCTACCCCTGCTAGAAGGTTACAGTGTATTATGTAGCATGCAATGTGTTATGTAGTGCTTAATAAA20.12004801920768307No Hit
GGGCTTCTCTCGCACCCCCAGCACCTCTGTCCCAAAACCTCATTCCCTTTTTTCTTTACCCAGGATTGGTT20.12004801920768307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA20.12004801920768307No Hit
GCTGCCCCACATGCATGTCTGCCTATGCACTGAAGAGCTCTTGGCCCGGCAGGGCCCCCAATAAACCAGTA20.12004801920768307No Hit
GCTGCCCCACATGCATGTCTGCCTATGCACTGAAGAGCTCTTGGCCCGGCAGGGCCCCCAATAAACCAGTG20.12004801920768307No Hit
CCCTAGCAGCACTCCACCTCCTATTCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
ATCCAAAAGAGCAATAAAAAGTTTTCAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
AACCAAGAGTAACATGAAAGGTTAGTAGCCAAGAACCAAGTGACGTTACAGGGAAAAAATTGAATACAAAA20.12004801920768307No Hit
GCTGTGAAAGGCCAGCTCTCGGCAGCCCCTGTGGTTTCAGCGCTGCCGCTCTGTGTCATTCAGGTTGTGCC20.12004801920768307No Hit
GCACTTTGAGAGGCGGAGGTGGGCGGATCATGAGGTGAGGAGATCGAGACCATCCTGGCTAACACAGTGCA20.12004801920768307No Hit
GGTGAAGAAAGCTAAATGCTGAGAAACTGAGCATCTATTGTCGTGTTTAAGCTTAGCTGGGTCCTTTCTAG20.12004801920768307No Hit
CTACAAGCATTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
ACTGAAGGTTTTACAGACATGTCTGTGGCACCACAGAACACGAACGGTCCCAATGGAAACCCCACGCCCAA20.12004801920768307No Hit
GGATTGACAAATCAGAGTTTGTTTTTGTTTTTTTGTTTTTTACTTTCCATAAAGTTCGTTTACCAGCATAC20.12004801920768307No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA20.12004801920768307No Hit
CCCTACACGACGCGGGGGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
TTATTATGTCCTACAAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
GCTACCACTCAGCACGTGTAAAATTTTTTAAATTTATAAATATTAAAATTTTAAACTTAAAAAAAAAAAAA20.12004801920768307No Hit
GTGAGCCAAGATTGTTTCACTGCACTCCAGCCTGGGTGAAAGAGTGAGACTCTGTCTCAAAAAAAAAAAAA20.12004801920768307No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
AAATAAAAGATCTTGACTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
GCCCACCTGTACCCAGGTGAAATAAACAGCCATGTTGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
ACGCGGGTGTTGGGACGTGCACCTGTAGTCCCAGCTTCTTGGGAGGCTGAGGCAGGAGAATTGCTTGAACC20.12004801920768307No Hit
CCCCCATCATGGGAATAAATTAATTTTCTCAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
GTACTTGTAGAGTGGTGCTGCTTTAATTCATAAATCACAAATAAAAGCCAATTAGCTCTATAACTAAAAAA20.12004801920768307No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACG20.12004801920768307No Hit
TCCCTACACGACGCGGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
GCTCTTTGTATGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
ATACTGGCCTCTGTGATTACATAGATCAGCCATTAAAATAAAACAAGCCTTAATCTGCCAAAAAAAAAAAA20.12004801920768307No Hit
GGGTTTTTAGCTAGTGTTTTTCCTCGCTTTTAATGACCTCAGCCCCGCCTGCAGTGGCTAGAAGCCAGCAG20.12004801920768307No Hit
GTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCC20.12004801920768307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT20.12004801920768307No Hit
CTCTTAATCAGTGGTGGAAGAACGGTCTCAGAACTGTTTGTTTCAATTGGCCATTTAAGTTTAGTAGTAAA20.12004801920768307No Hit
GGCTGTGGCCACCTGATGACCCTTTCCCTTTTTATTAAACCAAACACACCTATTTCCCAAAAAAAAAAAAA20.12004801920768307No Hit
GTCCTATACTAGACTGTAAGCTCTTTGAGGGCAGTCTGTCAGATTTATCTTTGTATCTTCCCCAGCGCCTA20.12004801920768307No Hit
GCATATTTTTTTGGAGTGCAGAATATTGTAATTAATGTCATAAGTGATTTGGAGCTTTGGTAAAGGGACCA20.12004801920768307No Hit
CTATTGTAATCTCTATTGGAGATTACAATAATTAAATCAATAAATAACTAAAACTTAAAAAAAAAAAAAAA20.12004801920768307No Hit
GGTCCCTTTTGTGCTTCCTTCCCCTCAGGTAGCCTCTCTCCCCCCGGGCCACTCCCGGGGGTGCGGGGGTT20.12004801920768307No Hit
TCCCTTGGGAGATCAATCCTCCGTCCTCCTGCTCTTTGCTCCCTGAGAAAGATCCACCTACGACCTCAGGT20.12004801920768307No Hit
CCCCCATCATGGGAATAAATTAATTTTCTCAATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
GTCTGGAGACAGGAGTGCAAAAAAGTCCACATCACACACCTGGCCAAAACTCGAGTACTATATTTTAAACA20.12004801920768307No Hit
GCCTATAATAAACTAAGTATGTGGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
GGGTACCCTTTGGGGAGCAGGAGGCCAATAAAAAGTTCAGAGTTCAAAAAAAAAAAAAAAAAAAAAAAAAA20.12004801920768307No Hit
GGTCCCTTTTGTGCTTCCTTCCCCTCAGGTAGCCTCTCTCCCCCCGGGCCACTCCCGGGGGTGAGGGGGTT20.12004801920768307No Hit
GTCAGAATTGTATTTTGGATTTTTACACAACTGTCCCGTTCCCCGCTCCACAGAGATACACAGATATATAC20.12004801920768307No Hit
AATATTGTTGTAATAGTCTGTTTCAATCTTAAAAAGAATCAATAAAAACAAACAAGGAAAAAAAAAAAAAA20.12004801920768307No Hit
GAACTAAGGTGTCTACCATGATTATTTTTCTAAGCTGGTTGGTTAATAAACAGTACCTGCTCTCAAATTGA20.12004801920768307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)