FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15538
Sequences flagged as poor quality0
Sequence length71
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA262416.887630325653237No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT370.23812588492727504No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.20594671128845413No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC310.19951087656068991No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.135152529283048No Hit
GTACTTAGCTGTACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTT200.1287166945552838No Hit
GTATTATATAACATATCTTGAGCATTCTTGACAGACTCCTGGCTGTCAGTTTCTCAGTGGCAACTATTTAC200.1287166945552838No Hit
ATGTGGTTTTATGTCGGAGAGATTATAGGCAAGCGGGGCATCATTGGCTATGATGTTTGAAGACCAATCTT190.12228085982751963No Hit
CATGGAAGACTGACTTTTTAATGCTGTTGTTTTGTGCTGAGATTTCTGTCCAAGAACAAAGAATATCTTTC190.12228085982751963No Hit
CTATAAAATTTCTCTTTTGAGTTTTATACCTTTGTAGCATTTTAGATGACATTGTGTTTGTACTTTGTTGT190.12228085982751963No Hit
AATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATG180.11584502509975544No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG180.11584502509975544No Hit
GGAGTAAAAAAAGAAAGTTATTTCTTTGTCTTAAAGAATTTTTAAAAAATTAGTCATGAGACTTATTCATC180.11584502509975544No Hit
ACAATAATCTGTACCTGGAACGAGGCGGTGATCCCTCCAAAGAACCAGAGCGGGTGGTTCACTATGAGATC180.11584502509975544No Hit
GAGTTACCCCAAAGGAATGGAGGAATCAGTAATCATCCCAGCTGGTGTAATAATGAATTGTTTAAAAAACA180.11584502509975544No Hit
CTCATAGGGGCCACTCCCTTGGACAGTGTCCCCTATGAGTTTATATTTTAACCACTGCTTCAAATCTCGAT180.11584502509975544No Hit
CTGCGAGCCCTAGCGCTGCGGCCCCGCTTCCTGGCGGCCGGGTCCCAGGCAGTTCAATTAACCTCCAGAAG170.10940919037199125No Hit
GAAGGAGATTGAGCGTCTGCAGAAAGAAATTGAGCGCCATAAGCAGAAGATCAAAATGCTAAAACATGATG170.10940919037199125No Hit
TGATAGTGGTTTTTGCCTTGAAGGAATTGCTTTGTATTTAGCTTTTCCCCCTCTAGATTTCTAGTTCCTTT170.10940919037199125No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10297335564422706No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA160.10297335564422706No Hit
CTTCTTAATAGAAGAAGTTCTACAAAAAAAACCTAGAGCCAACATATTTAATTGTGAATTTTTGAAAAGCG160.10297335564422706No Hit
AGATGGGTGGATCATGAGGTCAGCAGTTGAGACCAGCCTGAACAACATGGTGAAACCCTGTCTGTACTAAA160.10297335564422706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)