FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20417.028380634390654No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT161.335559265442404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.667779632721202No Hit
ACACGACGCGGGTATTATAGATGGAGTAAAGGTCCAGGTTCACACTAGAAGGCCAAAACTTCCGCAAACAT80.667779632721202No Hit
ATGTATGTGAGTCCCAGACAAGCAGGAAGCAGCAGCAAGAAGCAACTAGCACACAGAAACACCCGTGCGTG80.667779632721202No Hit
GGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTGATGCCTTGGACA70.5843071786310517No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG60.5008347245409015No Hit
GAAGCGCTCCCCTTCTTTGGCCAAGTGGATTTCACGGTGTCTGTTTCCCTGAAGTCCCTGGAGATCTAGCC60.5008347245409015No Hit
GAAATAGGTTTTGTCTGGTTGCATATAATCTTTGCTCTTTTTAAGCTCTGTGAGCTCTGAAATATATTTTT60.5008347245409015No Hit
CGCATGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAGTCTCTTGAACCTGGGAGACAGAAGTT60.5008347245409015No Hit
GCTCAGATCAGTAGTGTCAGCCAGAGCTTTCTCTGCAGACATGTAGGAAGTGGGTAGCTATTTTTCCCACT60.5008347245409015No Hit
CCAAGGGTTGACATGGTCTTCCCAGCATGTACTCAGCAGGTGTGGGGTGGAGCACATGTAGGCACAGAAAA50.41736227045075125No Hit
CTTGCCGACGGGGCCCTTATTTACCGGAAGCTTCTGTTCAATCCCAGCGGTCCTTACCAGAAAAAGCCTGT50.41736227045075125No Hit
GCTGCTATGCTGGGACTCATGTATGTCTACTGGACTCAGCTCAACATGTTCCAGACCTTGAAGTACCTGGC50.41736227045075125No Hit
CCAACCAAATTTCTGCATTCGAGTCTTAACCATATTTAAGTGTTACTGTGGCTTCAAAGAAGCTATTGATT50.41736227045075125No Hit
AGGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGT50.41736227045075125No Hit
ACGCGGGACATGGATCACAGTTTGCTTTGATCTTCTTCAATATGTGAATTTGGGCTCACAGAATCAAAGCC50.41736227045075125No Hit
CCAGTGAGCTCAGGTGTCTCAAAGAAGGGTGGAAGTTCTAATGTCTGTTAGCTACCCATAAGAATGCTGTT50.41736227045075125No Hit
GAATTTGGGTGGGAGAAAAGAAAGTGGGTTATCAAGGGTGATTTGAAATTTTCTGCAGCATTAAAGCTGGC50.41736227045075125No Hit
GAGAAAGCCAAGATCCACTACCGGAAGAAGAAACAGCTCATGAGGCTACGGAAACTGGCCGAGAAGAACGT50.41736227045075125No Hit
ACTGTATGAGGACTTTGTGCTGAAAATAGAGTATTTTTTTAAAGTAAGGCTGTCTTGGTTTAAAAGCAGAT40.333889816360601No Hit
GCATCACTGAAGCTGTTCGAAATGGCATACAAAAAGATGGCATCTGAGCGAGAAGGCTCTGGAAGTTCTGG40.333889816360601No Hit
ATTTATACAGACATCATTGTGTTGTATTTTAAGGAAAAATTTCCATTTACAATTTCACATTAACTCATATA40.333889816360601No Hit
GGTCCCTTTAAAGAGCAATCCTGGAAGAAGCAGGAGGGAGGGTGGCTTTGCTGTTGTTGGGACATGGCAAT40.333889816360601No Hit
TTCCCTACACGACGCGGGATGAAAGACTGAAAGCTTTTCCTCTAAGATCAAGAATAAGACAAGGGATCCCT40.333889816360601No Hit
GCTTACTAGGTTTTCCATTGTCACCATTGGATGAACCTCTGGTTTAGCCACAGTTGTGAAAATAAATGGAA40.333889816360601No Hit
GGATTATTGGTAACAAAAAGGCAATTGAACTTCTGATGGAAACCGCTGAAGTTGAACAAAATGGTGGTCTC40.333889816360601No Hit
GCCTTGGCCTCCCAAACTGCTGGGAGATTTTATTTTTTGAAACAGGGTCTTGGTCTGTTGTCCAGGCTGGA40.333889816360601No Hit
GGATTGGTTGAGCCCAGGAAGTCAAGGCTTCAGTGAGCCATGATTCTGCCACTGCACTCCAGCCTAGGAAA40.333889816360601No Hit
ACGCGGGGCCATTAAGAAGAAGGACGAGCTGGAGCGTGTGGCCAAGTCCAACCGCTGATTTTCCCAGCTGC40.333889816360601No Hit
CACGATGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCAGGCGTGGTGGTGGGCGCCTGTAGTCCC40.333889816360601No Hit
TCCCTACACGACGCGGGCAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCACAAAAA40.333889816360601No Hit
GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGGCC40.333889816360601No Hit
ATGTTGAGAATCAAGAGAATATGGTCCTCCATCATTTCTTGGCACCACAACTTCCAAAGGTAGTAGGATAG40.333889816360601No Hit
CTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGC40.333889816360601No Hit
GTAAACTTTACTAATACATTTAAATGGTGGTTAATTGGATAACATAATGAAGAAGAAGGAGGACAAGGACT40.333889816360601No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.25041736227045075No Hit
GCACAGGTGCTGTATGTCTTAGATATACAGCACAGGTGCACAGGTCTTAGATATACAGCTCAGTGAAGCTT30.25041736227045075No Hit
GATTCCAAAAGTAGTGCATGTGGAGAAAGAATCTAGACTTTCTTGTATACATTTTTCTCTTCTCCAGTAAT30.25041736227045075No Hit
GATCTTTTCCAAGGCTGTACAGACATGGCGGCGGCTTTTCGGAAGGCGGCTAAGTCCCGGCAGCGGGAACA30.25041736227045075No Hit
ACCTAGCTCTCCTCAAAAAGGAGCATCAGGAGGAAGTCGATGGCCTACACAAGCATCTGGGCAACACTGTC30.25041736227045075No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA30.25041736227045075No Hit
GCAACAGAGCCTGTCTGTCCCCATGAGCGGAGTCTGTCCTCTGCTCTTCTGCAGTCAGGTCACTGCCTACT30.25041736227045075No Hit
CCTAGCACAGTGCTTTGCACATTCTGAGGATCATTAAGCAGTTGTTGAATATATGAACAGGTACATCATAC30.25041736227045075No Hit
TGTCAATATGTGATATAGAAAAGAGATACGTGAATTTTTTAGCTAAGCTTGACAGATTGAAAGACAAGTGT30.25041736227045075No Hit
AAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG30.25041736227045075No Hit
TACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCC30.25041736227045075No Hit
GTCAGATTGTCATGTTGGAAGCCTCTGCAGAGAACATTTTACAGCAGGACTTTTGCCATGCTATCAAAGTG30.25041736227045075No Hit
ACCATCTCCCAAGCCATGCTGAATGCTGTGGACCGGGATGCAGTGTCAGGCATGGGAGTCATTGTCCACAT30.25041736227045075No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.25041736227045075No Hit
ACGCGGGAGAATTGACAGAGATAGTTACTCTCCCCGGTGGAAGGGAAGATGGGCAAATGATGGTTGGAGAT30.25041736227045075No Hit
GTATGAATTTCTGTCCTTTGTACGACAGCAGACTCCTCCAGGGCTCTGTCCACTTGCAGGAAATTCAGTTC30.25041736227045075No Hit
CATACAATTGTTTTGAAGAATAAAATGCAGTTTATCATTAATGTTTTAGGAGACTTAAAACTTCTCTTGAC30.25041736227045075No Hit
ATATTGATATGTATTACACTTACACATACAAACACATATAAGAGGGGGTGAAAACCGTAGCCTTTGCATTC30.25041736227045075No Hit
CCTCACCGCTCCCAGCAACTGAAAGGACAACACTATCTGGAGCCACCCACTGAAAGGGCTGCAGGCATGGG30.25041736227045075No Hit
ACCTTGGCCTCTCAAAGTGCTGGGATTACAAGCGTGAGCCACTGCGCCTGGCCAAATAATTTTTAAAATTT30.25041736227045075No Hit
GAACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.25041736227045075No Hit
GGATGGTTGTGGCCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.25041736227045075No Hit
TGTCTTCATAGATCATATTTTCTTAGACAATGTTTAAATAGATAAATTGCTAATATTGAGAATGTGTCAAA20.1669449081803005No Hit
GTTTTTATCCTTTTTTTTTGGGTAGCATAGTGAGACCCTGTCTCTACAAAAAGTTAGACGCTGTAACTGAA20.1669449081803005No Hit
GCTATGGGTGATTCAGAATATTATGTTTAGATTCCCATTTGTTAAGTTTATAAGTTTTGTGGGGAATTATG20.1669449081803005No Hit
CAGAGAAGCCATGTTTGCACTTCCAGCTGAGAAAAAATGGCAAATATACTGTAGCAAGAAAAAGGACCAGG20.1669449081803005No Hit
CCCCCACTCAAGTCAGAGAAACATGAGGAATAAAGGTCACATGCAGATGCAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GCATTCAGGTTGGCAGGAACATCATTCATGGCAGTGATTCAGTAAAAAGTGCTGAAAAAGAAATCAGCCTA20.1669449081803005No Hit
GTTTGAACCTGGGAAGCAGAGGTTGCAGTGAGCTAACATCATGCCACTGCAATCCAGCCTGGACAACAGAG20.1669449081803005No Hit
CATTTAAGTGTGTGTTAAACAACTACAGAATACTAAATAAAAAGTTTGGCCAAAACCAACCATGAAGCTGC20.1669449081803005No Hit
ATTTTTGCCCTGAATTAAGTGTTAATTTGATGGAAACTCTGCTTTTAAAATCATCATTTACTGGGTTCTAA20.1669449081803005No Hit
GGTCTACATCAACTATTACGACATGAACGCGGCCAATGTGGGCTGGAACAACTCCACCTTCGCCTAAGAGC20.1669449081803005No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACAC20.1669449081803005No Hit
GTGTGGCTTGGAGCCAATGGGAAGGTGGCTGTGATTGTCAGCAATGACCATGCTGGGAAACTGTGTGGGGC20.1669449081803005No Hit
AGTTATTAGTTTTTAAAATCAGTACTTTTTAATGGAAACAACTTGACCAAAAATTTGTCACAGAATTTTGA20.1669449081803005No Hit
CTTCAGATCAACTCGTTTTTATAATGGTGTTGTTGTGGAACTGAAAGATTGGGGATGATTTGGTTTTTTCC20.1669449081803005No Hit
TCCCTACACGACGCGGGCTTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GGGTTAAAGAGGAATGAAGCCTTTTAATTCAGAAATGGGGCCAGTTTAGAAAGAATTATTTGCTGTTTGCA20.1669449081803005No Hit
GCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTGAAAGTTCATATCTGGAGCCTGATGTCTTAACG20.1669449081803005No Hit
CACCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTCCCCCTCAAACCTAAA20.1669449081803005No Hit
CAAGTGACCCTTCCCTTCCCTCCACCACACCACTCAGGGGCTGGGGCTTCTCTCGCACCCCCAGCACCTCT20.1669449081803005No Hit
GCCTTAGGATGCAGCATGCCTTCAGGAGACACTGCTGGACCTCAGCATTCCCTTGATATCAGTCCCCTTCA20.1669449081803005No Hit
AAACATCAGTTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
TCCCTACACGACGCGGGAGGCGGAGGGTCGGTGATGTGGAACCGTCACGCAAACCCAAGAGGCGGAGGGCC20.1669449081803005No Hit
ATAAAGTGGTTTGAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GTCTCATGGGATTGTCTGGTTTCCCTTACAGGGCCCCCTCCCCCGGGAATGCGCCCACCAAGGCCCTAGAC20.1669449081803005No Hit
GTTGCAAATTAACAACTTGCAAATGAGGTGCAAATAAAATGCAGATGATTGCGCGGCTTTGAAAAAAAAAA20.1669449081803005No Hit
GTTTCTAGGGTAAGGGACCTGCAGGTGTAAGCATAGATGAAATAACTGTCCTGTCACATGTGCAGCAGGCC20.1669449081803005No Hit
GATTATACAGGCAGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GAAACAAATGAAAGGATATAATGAAAGTATTACTTGGAGCCTAAGATGCTAATGCTAGGGAGAAACGGGAT20.1669449081803005No Hit
GGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTGGGCGTGGTGGTGAGCGTCTGTAATCCCAGCT20.1669449081803005No Hit
GAGCTAAAACTGTGAACCTAACTTTCTCTTATTGGTGGGTAATAACTGAAAATAAAGATTTATTTTCATGC20.1669449081803005No Hit
AGATGTCAAAACATTTCGAGAGAGAGGCAGCTTGCAGAAAAGCAGAAAGGTTGAATTCAGGGGTCAGCAAA20.1669449081803005No Hit
GTAATCAACTGAGCTAGTTCCAATAAAGTTAAGCAGGTTTAAATCCACTTTGTGCCTATCTTTTCACTGAC20.1669449081803005No Hit
CCACTGGGGGTACCTGAGCCTGGATTAGAGGGCAGGGGGAGGATATTGCCTAGCCAAAGTGGGTGTTCAAT20.1669449081803005No Hit
CATTCAATTGTTGTTATTATGTCCTACAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
CATATGTGTAATTGGAGCCCCAGAAAAGAGGGAAAGGGGAGAACAGAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GAGTACTGATATGTATATAATAAAAATTATCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GTTTTCTGTACATAAAAATAATTGAAATAATACAAATTTTCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GGCTAACACGGTGAAACCCCATCTCTATTAAAAATACAAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GATTAGGTTATGGTTTAATGTTACAACAACTATTTTTTAAGAAAAACAAGTTTTAGAAATTTGGTTTCAAG20.1669449081803005No Hit
ACGTAGGCCCTGTGCCCTCCGGCCACCTGGATCCCTGCCCCTCCCCACTGGGACGGAATAAATGCTCTGCA20.1669449081803005No Hit
TGTACCTACTGAGTGCTATGTGATTTTTAAAAATGTATTACTGTAGAATGCTTCTGCAAATTCAATAAAGT20.1669449081803005No Hit
GTGCTTAACCTACCTCCCAACACCATTATGGAATACAAAACTCACACCGACAGCATCAAAATGCCAGGCAG20.1669449081803005No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
TTCCCTACACGACGCGGGGATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAA20.1669449081803005No Hit
CTCTTAAGCAGCATGGAAAAATGGTTGATGGAAAATAAACATCAGTTTCTAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
CAAATACACAGAGGTCCTCAAGACCCACGGACTCCTGGTCTGAGCCCAATAAAGACTGTTAATTCCTCAAA20.1669449081803005No Hit
ACTAATAGAATAAAGGCAGTTTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
ATTGAATAAATATCTTAAGCTAAGCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
CTATAAAAGAGCCTGTGATTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit
GCTTAATCCTGTTTACAATGTGAGCTTTTTGTGCGTCTGTGAAATGTTTTACAGTGTTTCTCATCATCTGT20.1669449081803005No Hit
GAATAAAAGTGCACACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1669449081803005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)