FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53421.716144774298495No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC160.6506710044733631No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT130.5286701911346076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.5286701911346076No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG120.4880032533550223No Hit
GCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATT100.40666937779585194No Hit
ACACTATTTTGTTTTATTTGTCTTATTAATATAAGAAGGCAGGAATGTCAGGCCTCTGAGCCCAGGCCAGG90.36600244001626675No Hit
GACCGCAGCACTGGTTTGGACCGTTACTCTGCACATGGACCAGAAAAAGTATATGGGACCTTAAGCTCACC80.32533550223668156No Hit
GCTTCCTTCAGCAGCGCAGGCGGTGGTCCCTGAGGCCCGTGGAAGGAGTCAAACTTGCGGGAATTTTGCAG80.32533550223668156No Hit
TGGAGAAACCCTGTCTCTACTAAATATACAAAATTAGCTGGTGTGGTGGCGCACGCCTGTAATCTCAGCTA70.2846685644570964No Hit
GTTTAGAAAAATAAATATACAATAAAAGTAAACACATACACACAAAACAGCAAACTTCAGGTAACTATTTT60.24400162667751116No Hit
CTCTAGTAGAACAAAAAACCACATATGCATAAAGTTGGAACCCCCAAAAGGTGACACTTAACAATGTACGG60.24400162667751116No Hit
GATATTTCAAATTGTTTTCAGTGAAAACAAGTCCTTCCATAATAGTAACAACTCCACAGATTTCCTCTCTA60.24400162667751116No Hit
TTCCCTACACGACGCGGGGCATTAACAGTGACTTTGACTTCCACCTTAGAATACCAGAGAAAGAAGAGTCT60.24400162667751116No Hit
CCTTTTCTAGCAGCTGCCAGTTTGGAGCATTGCCCTCTAAGAGCTTTAAAACTATTTTTTTACATGCCTTA60.24400162667751116No Hit
GGAGAGAAGTTTGTCATGCAGGAGGAGTTCTCGCGTGATGGGAAGGCTCTGGAGAGGTTCCTGCAGGATTA60.24400162667751116No Hit
GAGACACAAAGAGAGAGAGACAGAGACACAGAGAGAGAGAGAGAGACAGAGACAGATAAAGAGGAAGGAAG60.24400162667751116No Hit
GTGATCCACCTGCCTCGGCCTCCCAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCCTAGCCTAGATGTT50.20333468889792597No Hit
GAACTCTCTGTGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTTACAGGACTCAACATACT50.20333468889792597No Hit
CCACAGCTGCTCCTGCTGCTGCTGCAGCCCCAGCTAAGGTTGAAGCCAAGGAAGAGTCGGAGGAGTCGGAC50.20333468889792597No Hit
GGGTGACCGAGCATTTCTGCCCCTGTGAATGTGGCACTAACACTGTGCACTGTCTCCACCAAGCAAGGTTT50.20333468889792597No Hit
AGGATAGCATTGGGAGATATACCTAATGCTAGATGACGAGTTAGTGGGTGCAGCGCATCAGCATGGCACAT50.20333468889792597No Hit
CTTTGTGCCCATTGACCTAGACGAGTGGTGGGCGCAGCAGTTCCTGGCGAGAATCACCAGCTGTTCCTAGT50.20333468889792597No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA50.20333468889792597No Hit
GTTTTGAAAAGCTATTAGCCAGGATCATGGTGTAATAAGACATAACGTTTTTCCTTTAAAAAAATTTAAGT50.20333468889792597No Hit
GCACGATGCCATCCTTGAGGACTTGGTCTTCCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAG50.20333468889792597No Hit
GCACCTGGGCAACAAGAGTGAAACTCCGTCTTAAAAAATATAAGAAATAAAAAAATAAAAACCTGTCCCCA50.20333468889792597No Hit
GATTATAGGCATGAGGCACCATGCCCAGCCTGCTTTCTTTTTTAAGTGAATTTTCATCTTTCATTTTCTAA50.20333468889792597No Hit
CCACTGCCACCGAATTTCAAGAGCCCAAGCACAGAGCCCAGAACTAAATTGTGAGGGTTTAAAAAAAAAAA50.20333468889792597No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.20333468889792597No Hit
GCTCCAGGGCTACCTGCGATCTGTGTTTGCTCTGACGAATGGAATTTATCCTCACAAATTGGTGTTCTAAA50.20333468889792597No Hit
GGTAACTTTTTTATATAGTTGTAAAATTCCATTATATTCCATTGCCAAAGAAACATTAAGAACTTTGTATA50.20333468889792597No Hit
TGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTT50.20333468889792597No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAAACATCCACTTAAGTTCTTTGATTTGTACCATTCCT50.20333468889792597No Hit
GGCAGAAAGGGGACTGATGATTCCATGACCCTGCAGTCGCAGAAGTTCCAGATAGGAGATTACTTGGACAT50.20333468889792597No Hit
ACTCCCAGGCCAGTGAGCTTTACTTGCCGTGTAAAAGGAGGAAAGGGGTGGAAAAAAACCGACTTTCTGCA50.20333468889792597No Hit
GAACGAGACTCTGTCAAAAAAAAAAAAGTAGAAACAACAAAAAGTCAAAGAGGGGGATGGAGTTTGGAGTT50.20333468889792597No Hit
ATCCCACCACTTTGGGAGGCTGAGGCGGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGGCAAC50.20333468889792597No Hit
ACAATATTGTATACTACCATAGTGAGCCATGATTTTCTAAAAAAAAAAATAAATGTTTTGGGGGTGTTCTG50.20333468889792597No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGGA40.16266775111834078No Hit
GATATTTTGTGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16266775111834078No Hit
CAGCCAAATCTTAAGTACTGTGTGACCACTCTTGTTGTTATCACATAGTCATACTTGGTTGTAATATGTGA40.16266775111834078No Hit
GAAACTGAGTGAGCAGAGGAAAGGGGACAAGATCCTCCTGAATGCCTGCTGCCCAGGGTGGGTGAGAACTG40.16266775111834078No Hit
CGATCATGTTTCCATGCTGTAAAATGTCTTATTAGCAATACAGATTAAATTTTACCTTGCACTGTTAACTC40.16266775111834078No Hit
TGTTTGCCTCTCTTGAAAATGGCAGGTGCTCTTGTTTTACCCATGTTGGGTCAGCCTGAAACTGCCAACCA40.16266775111834078No Hit
TCCCTACACGACGCGGGGCAGCTTCAGAAAGCAGAAGTCAGCAGAAACCCAGGAATTCAACACAAAAAAAA40.16266775111834078No Hit
CTTTGTCTGTACATACTGGCCTCTGTGATTACATAGATCAGCCATTAAAATAAAACAAGCCTTAAAAAAAA40.16266775111834078No Hit
GGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTT40.16266775111834078No Hit
GCCCCTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAAGCACGAGGAGAACCTGA40.16266775111834078No Hit
AGATAAATTGTAGGGCTTTAGCTTTCTTATTTTTGTCAAAAGTTGGTGTTGACATACATTCCCTCTAATTT40.16266775111834078No Hit
GCTTCTCCCGCCATTGGCCTTAACTGGCCCTCGGGCCCTCTCTCCGCCCCGGACAGGGTGGCACCCACCAC40.16266775111834078No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16266775111834078No Hit
GGGCCTCCAGCAGGGTCAGCCATTCACACCCATCCACTCACCTCCCATTCCCAGCCACATGGCAGAGAAAA40.16266775111834078No Hit
CCCTGGAGCTGCCAGCACCCCCGATCCAGATTCTCCCTGGGTCGGGGACGTTGGTGGTGACACAGACCTCA40.16266775111834078No Hit
GTGTACAAATTTATTGATGTTGTGAAATCAATGTGTACAAGTGGACTTGTTACCTTAAAGTATAAATAAAC40.16266775111834078No Hit
CATCAACGAGGGGAAGCTGTGGAAACACATCAAGCACAAGTATGAGAACAAGTAGTTCCTTGGAGGCCCCC40.16266775111834078No Hit
GGTCTTGAACTCTTGGCCTCAAGTGATCCTCCCACCTCAGCCTCCCAAAGTGTTGAGATTACAGGTGTGAG40.16266775111834078No Hit
GGATTGCATCCTGCTGGGGCTGAACATGCCTGCCAAAGACGTGTCCGACCTACGTTCCTGGCCCCCTCATT40.16266775111834078No Hit
GAATCATTTGGGTCCCAGAAGACAGAGAACAAAAGGATTGGAAAACTCATCTATTTTTTTGTTATTAAATA40.16266775111834078No Hit
ACGCGGGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16266775111834078No Hit
GGCCAAGGTGTGCGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGACCACATGGTGAAACCCCACCT40.16266775111834078No Hit
TGTGCACCACCACACCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCGCCACGTTGGCCAGGATG40.16266775111834078No Hit
CCCTCCTGGGCCTCATTGGGTATCACCTATACAGAAAGGCCAATAGACCCAAAGTCTCCAAAAAGAAGCTC30.12200081333875558No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA30.12200081333875558No Hit
ACCTAGGACATTCTTAGTTTGACAGAGCAAAACACTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
ACTGTTGAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
CTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTG30.12200081333875558No Hit
GGTCATCACTAACCAGATTTACTTGGAGTACATGTGAAAGAAAACGTCAGTCTGCCTGTAAATTTCAGCAA30.12200081333875558No Hit
GTTCCAATCCCTATACATTGAAGCTTTGCTTTTCTACATCATCCCATTTATAAGAAGAGAAGAGCATGTTA30.12200081333875558No Hit
CAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGATGCTATAAAA30.12200081333875558No Hit
GTCATTAACGTTTTTTATTTAGTTTCTTTAGCCAATAGTGGCTGAGTTTCGCACTTGATTTTCAATATTTT30.12200081333875558No Hit
CATTTCCAAACCATCAGCTGCTTTTAAAATAAGATCTCTTTGTAGCCATCCTGTTAAATTTGTAAACAATC30.12200081333875558No Hit
CGCATCACTGCACTCCAGCCTGGGTGACAGAGAAAGTCTCCAACTCGGAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
GGGTTTCAGTTTTTTCATAGATGAATAGTCAATTGATTTAGCACCATTTATTGAAAAGATAATTCTTTCCC30.12200081333875558No Hit
GTCCTATGAAGACCGCTGCCATTGGTGTTGAGAATAATAAAGCTCTGTGTTTTTTTCTAGTGAAAAAAAAA30.12200081333875558No Hit
CTCCAGCCTGGCAGACAGAGTGAGACTTCGTCTCAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
CAACATGGAGAAACCTCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCACACGCCTATAGTC30.12200081333875558No Hit
CTTTAAAATTGTCCCAAACAGAAAAATAATGGCTATCAGAAGTATGTTTTGTTTTAGTGTGAGTTACCGTT30.12200081333875558No Hit
GATCATGCCACTGCACTCCAGCCTGGGAGACAGAATGAGACTCTATCTTCCCCCCCGCAAAAAAAAAAAAA30.12200081333875558No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTCTGTATGTCTCCATCTATTGATGAGGGTCTTA30.12200081333875558No Hit
GAACTCCTTAATACAATTATGAATCTGTGAATTCTCTTAAGTCTTAACATATAATTTAATTTCTAAAATGC30.12200081333875558No Hit
CTTCTGTCTGTCAGGCCTTGAGACTTGAAACCAGAAGAAGTGTGAGAAGACTGGCTAGTGTGGAAGCATAG30.12200081333875558No Hit
GATCCAGTGTATAAATCCAATATCATGTCTTGTGCATAATTCTTCCAAAGGATCTTATTTTGTGAACTATA30.12200081333875558No Hit
GTATAATTATTTGTTACTTTTGATTTGAGAACTCTACAAATAAAAGTGCTGGGACTAGAAAAAAAAAAAAA30.12200081333875558No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
TCCCTACACGACGCGGGCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
GTGACTTTAAGTGAAACAACTACGGTGAAGGGGGTCCTCAAATAACATCATTTTATTATAACATTAGTAAA30.12200081333875558No Hit
GAAAAACAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
ATTCTTACTGCCTTATTTTATTGACAGTCCTAAGAAAAAACAGAAACGTGGTGTGATCTGATCAAACTATC30.12200081333875558No Hit
GTAAAATATAATACCATCTTCTCTGTTAATACATAATTGCTCCAAGCTTCCTGTAAACTATAAGAATATAT30.12200081333875558No Hit
GATGCAAGCATTTTGAACTGGGAGGAGATAAGAAGAGAAAGGGCCAAGTGATCCAGTTCTAAGTGTCATCT30.12200081333875558No Hit
CCTCGCCCCAGCCTCATTCCCCATTCTGTGTCCCATGTCCCGTGTCTCCTCGGTCGCCCCGTGTTTGCGCG30.12200081333875558No Hit
ACGCGGGGAATCCTCTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
GGGTCCACGCCACGTGGCCTGGGCTGCCATCCTGCCGTCCTCCCACTGGCATCCTGGCAAGGGGGCGTTGC30.12200081333875558No Hit
TTATTGTTTGTTGAATATAGTGATATCTTGGAGTCAATAATTTTATATTTAAACAGCTTTATTAATTTACT30.12200081333875558No Hit
GTTTGAAGACCAATCTTTAACATCTGATTATATTTGATTTATTATTTGAGTGTTGTTGGACCATGTGTGAT30.12200081333875558No Hit
CACGGGGTAACGGGTCTAATCGTGTAGGAATAAAGCTGTATTCCAGTGCTTCAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
ACAGATTGTTCCTAAACCAGAAGAGGAGGTTGCCCAGAAGAAAAAGATATCCCAGAAGAAACTGAAGAAAC30.12200081333875558No Hit
ACATGGTAAAACCCCGTCTCTACTAAAAATACAAAAATTATCCAGGCATGGTGGCGCGTGCCTGTAATCCC30.12200081333875558No Hit
GCTATGGGGTGGTCAGGGGATTCCGGGAGGTGCATGGGTATGTTGCAGACCCCCCCAGGACAAGGGTGGCT30.12200081333875558No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGA30.12200081333875558No Hit
AAATTATTTTGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
GCTCAATTTGCTGCTCAGATATTTGGGTTAAGCAACCATTTGGTATGGAGCAAACTGCGAGCAAGCATTTT30.12200081333875558No Hit
TGTTGTTTGTGCAAAAACATAATATTCTTACATCAAACCATACAACAAAATAAATTACAAGTGGATTTACA30.12200081333875558No Hit
GCATATGTTCAGTGCATGCTCACAGCTAGGAATTAAATTAATTTTTATTTATAGAAAAAGAGTGAACTTTG30.12200081333875558No Hit
GGACTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
GATGGGTGACCACCTGACCAACCTCCACAGGCTGGGTGGCCCGGAGGCTGGGCTGGGCGAGTATCTCTTCG30.12200081333875558No Hit
CCTCAAGGGGGCCTGGAACCCGGGTGCTGGGGTCATGCTGCCTCCGTGGCTCCAAGGTGAGGGTCCTCTTC30.12200081333875558No Hit
GCTTTGACTCCAGGATCCCTGGAGTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGA30.12200081333875558No Hit
GAATTGATATTTAGATGGTTTTTAATACTCAGCAGATTGTCTTCCTTTATATTGTGTCTTTTTTATGTTGC30.12200081333875558No Hit
GTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12200081333875558No Hit
GAATGTAATTGAACCCTTACCTTACACCGCGTATAAAAATTAACGCAAAATGAATCAAAGACCTAACTATA30.12200081333875558No Hit
CAATACCATTCAGGACATTGGCATGGTCAAAGACTTCATGACTAAAACACCAAAAGCAATGGCAACAAAAG30.12200081333875558No Hit
GATTCTTCATTCTGTATGTGACTATTTATATAACATTAGATAATTAAATAGTGAGACATAAATAGAGTGTT30.12200081333875558No Hit
ACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGTTGGCAGGCGCCTGTAGTCCC30.12200081333875558No Hit
GTTTTGGACAGTCCAGCAAGTAAAAAAAAGAAATAGTCAAATAAATTATCTGAAAAGAAACAGGTGAAAAA30.12200081333875558No Hit
CCTCTGCCCGCCTCCCGTGAAGAAGAGGAAGAGAAAATGCCTGCTGTTGTAAATGTCTCAGCCCCTCGTTC30.12200081333875558No Hit
GGATGATCTCAACAAGAGCTGTATTTTAAGTATTTAGACAGTTCTTTGTTAGCTGGTTTCTAGTTGGTTAT30.12200081333875558No Hit
GACCAACATGGAGAAACTCCGTCTCTACTAAAAATAAAAAATTAGCCGGGCCTGTAATCCCAGCTACTCGG30.12200081333875558No Hit
CATGGTGAAACCCCGTCTCTACTAAAAATACAATTGTGCCACTGCACTCCAGCCTGGGCAAAAGAGCGAGA30.12200081333875558No Hit
GTCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGA30.12200081333875558No Hit

[OK]Adapter Content

Adapter graph