FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5601
Sequences flagged as poor quality0
Sequence length71
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1212.1603285127655774No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571.0176754151044456No Hit
TTTGTAAGACTAATTATGGTAACTTAGCTTAGTATTCAATATAATGCATTGTTTGGTTTCTTTTACCAAAT160.28566327441528294No Hit
CCTACACGACGCGGGCATTCTTTTTTTGTTTGTTTGTTTGTTTTTTGACTCCGTTACCCAGGCTGGAGTGC140.24995536511337263No Hit
CTGTCACCAAATTTGTACCTCTAAGTACATATGTAGATATTGTTTTCTGTAAATAACCTATTTTTTTCTCT140.24995536511337263No Hit
ATGTACACCTGATAGCAAGAAGCTAATTCATATGCTTTAAACCAATGAAGGCTTGTCAAAGAGATTTAGTT100.17853954650955187No Hit
ATAATATACTTAGCATAGTATCTGGTACTTGTAAAGTAATTAATAAATGTTGGATCTTATTTTTGTTCTAA100.17853954650955187No Hit
CCATGGACGTGGGCGAACTTCTGAGCTACCAGCCCAATAGGGGCACAAAACGTCCCCGGGATGATGAAGAG100.17853954650955187No Hit
CATTATTAACAAAAAGATGAAAATACCCTATCATCTGGAAATGAAAAACAACCTATTAACTCTTGGATGAA100.17853954650955187No Hit
TTCCCTACACGACGCGGGAAAGTGAAGAACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.17853954650955187No Hit
GGCTTTACTATGTATTAGCCTTTTGTTGGACTGTGTATGTATTTTACCATATGGGTTTTAATGATAATGGT100.17853954650955187No Hit
GCTAGAAGAGGAAGAACAGCTTGAAGCTGCTGGACTAGAGAGAGAGCGGAAGATGCTGGAAAAGGCTCGCA100.17853954650955187No Hit
CATATTTATGCTGTCTGAAGGTCACGATCATGTTACCATATCAAGCTGAAAATGTCACCACTATCTGGAGA100.17853954650955187No Hit
CTAGGAGGCAGAGGTTGCAGTTTGAGACACTGCACTCCAGCTTGGGTGACAGAGCAAGACTCTGTCTCAAA100.17853954650955187No Hit
TCCCTACACGACGCGGGACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.16068559185859668No Hit
CTTTTTTAGCTGCAAGTCAAGGACTAGGTTGATGATGCCCATGACCTGTAATTGTAAAGAAGCTTGGACAT90.16068559185859668No Hit
GTAGTATGTTTGTAAGCTATTCTGACAGAAAAGACAAAGGTTACTAATTGTATGATAGTGTTTTTATATGG90.16068559185859668No Hit
GTCCATACCTTGGGTGTTTGTATTTGGACAACCACAGACATTGCCAAGAAATGTATCTGTTCTACTTGCAC90.16068559185859668No Hit
GTTGCAATATGTGAAATCAGAGGACCAAAGTACAGATGGAAACCATTTCCTACATCACAAAAACCCAAGTT90.16068559185859668No Hit
TTGATGAGGGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.14283163720764147No Hit
ATTGTAAAGAAACTATGCTCTATACGTTCAACGAATTAAAAGGTGGAGAACTTCAGCAGAAATTTGACACT80.14283163720764147No Hit
TGTCTGTGTGTTTAAATGTTTACCAGCAAATTGTCTTATCATGTTAATGAGAATGTTCAATGCCTGTGTGG80.14283163720764147No Hit
CGGGTGTATTATCCAGGTACTCGTACACTATTTTTTTGTACTGCTGGTCCTGTACCAGAAACATTTTCTTT80.14283163720764147No Hit
GAACTAAGAAGACGAAGGAGAGGAAGAAGAAAGAAGAAGAAAATAAGGAACAAGAAAGAAGGAGAAGAAGA80.14283163720764147No Hit
TATTTATGCTGTCTGAAGGTCACGATCATGTTACCATATCAAGCTGAAAATGTCACCACTATCTGGAGATT80.14283163720764147No Hit
GTCCATCCTTGTTATAAAAAATTTTTTTACAATTGTAATTATATTGATGTTCATATTGTGTAAAATAACTC70.12497768255668631No Hit
ATATTGACATTTGTAGTTGAGCGGGGGGTTTGGTTTGCTTTGGTTTATATTTTTTCAGTTGTTTGTTTTTG70.12497768255668631No Hit
CATTGGCAGGGGTACCAAAGTGATCCTCCATCTTAAAGAAGATCAGACAGAGTACCTAGAAGAGAGGCGGG70.12497768255668631No Hit
TCTCACTACTCTGGAAACATAACTGGCTACATAGAAATACTGACTAATATAAAAACAATGCTTATCTCTAA70.12497768255668631No Hit
TTCCCTACACGACGCGGGCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.12497768255668631No Hit
GCTTGATTTAAATACTGTGAACTCTAATAATGTGGAAAATATTTTTCAACTTTAATTTTCTGAAGTATAAA70.12497768255668631No Hit
AAAAGAAAGAAGAAAATAATAAGGAACAAGAAGAAAGAGGAAGAAGAAAGGAGAAAAGAAAGGAAGAAGAA70.12497768255668631No Hit
GGTGGCAGGTGCCTGTAGTCCCAGCTACTCAGAAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGG70.12497768255668631No Hit
GTACACACATGAATACAAAGGACCAAAAGCAGACTTAAAGAAAGATGAGAAGTCTGAAACCAAAAAGCAAC70.12497768255668631No Hit
GGATTTGTTCCTTGGTAATTGTTTGGTGTGTCATCCTGTAAATATTCATGATAGTCTGTTTATATCCTTTT70.12497768255668631No Hit
GAAATAAACTGCTTTCTTAATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10712372790573112No Hit
ACCACTCACCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTCCCCCTCAAA60.10712372790573112No Hit
GTTCAACCTAGATTTGGTAGAGGACATAGCTGAAATCAAAGGAAAAGTTGCAAACCCCAGAATCAGCATTT60.10712372790573112No Hit
GTATGTTTTGTTTTGCAGACTTTTTGCATACTTATTTCTACATGGTTTAAATCGACTGTTTTTAAAATGAC60.10712372790573112No Hit
TCTCTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGAAAATAAGAATCTGAATAT60.10712372790573112No Hit
GTAAGAAATATCCACAGTGAAGCACCGAGAAATGAAAGGATGAAAAAAACATAGTGTAATGGAGACAAGGG60.10712372790573112No Hit
CTATAAAGCACTTAGAAGAAAACATAGGAGTAAATCTTCATGACTCCGTGTTAGTTAGGCAAGGCCTTCAA60.10712372790573112No Hit
TCTTTGCAGATGAAAATAAAGGTGTTTTTGGTTAACTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10712372790573112No Hit
GGATACTAGGGGAAAACAGATGCTATCTCAACCTTTCCTGCAGCTCACATTCATTTCAACTCATATTTTTT60.10712372790573112No Hit
CCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAAC60.10712372790573112No Hit
TATTTCAAGTCTTTAGATGTGAATAATTTGAGCTTGGTGTATACATTTCTTGTGACTTGCGTAACTTTTAA60.10712372790573112No Hit
ACACGACGCGGGATTTCAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10712372790573112No Hit
CTTCTCCTCCATGGTCTGGAAGCGGCCATGGAGGAGAAGAAAGCATTCATGGGACCACTGAAGAAAGACCG60.10712372790573112No Hit
GAATAAAGTGATGAAGTTGCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10712372790573112No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10712372790573112No Hit
ATTTATAATTGCCAAAAAGTAGAAACAACTGAAATGTCATCAGCAGATAAATTGATTAAAAAAAAATGTGG60.10712372790573112No Hit
CATTTGTAGAACAAGTAGTAAATGAAAAAGTCAAACACTTAGAAGATACCTTAAAAGAACTTGAATCTCAA60.10712372790573112No Hit
GTTTATAGCTACTGTGACAGAAACAGAAAAGCAGTGGACAACACATTTCAAAGAAGATCAAGTTTGTACAT60.10712372790573112No Hit
CCCTGTCTGACCTTATGTTTGGCCTCTCCCTCAAAGTCACTAGAGATGAGTCATGATGAGCATAAAAAGCA60.10712372790573112No Hit
CATCTGATCTGTATAAAAATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10712372790573112No Hit
TTTTGAGACTGCAGCCTATTAATAAAGTTAACATAACTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10712372790573112No Hit
GATAAATTTAAACCTGAAAAGTAGGAAGCAGAAGAAAAAAGACAAGCTAGGAAACAAAAAGCTAAGGGCAA60.10712372790573112No Hit
CCCTTATGGAGATCACTGGGAGGGAACTGAAATTAATAATAATAGTAATATATTAGTGTTGTAATATGATA60.10712372790573112No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)