FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5074
Sequences flagged as poor quality0
Sequence length71
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1663.2715806070161606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230.4532912889239259No Hit
ATAATTGACAGGATTATTGGAAATTTGTTATAATGAATGAAACATTTTGTCATATAAGATTCATATTTACT140.27591643673630273No Hit
TTATTATAGCTATATTCTATATTTTCAGAAAGTAGAGACATGAAAGACATTTTTAAAAACTCAATGCAGTT130.2562081198265668No Hit
GTTGGAAACTCCGTTGTGCTTTTTTCCACATGGAGGAGAAAGAAGAAGTCAAGAATGACCTTCTTTGTGAC120.2364998029168309No Hit
TAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTTTTTTTTCCTTTTTTGTCTAT110.21679148600709497No Hit
AATTATGAGAAGCAGTCTTCATAAGAAGAATTACATTTTTATATGTCTGTTAAGAAATTAAAAAAAATTTT110.21679148600709497No Hit
GCCCTGGAGCCAGGGCAGAGGAATGTAGGGCCTGCATGGAGAAGGGTTCTGCCCTGCCTGAGGAGGAGGAC110.21679148600709497No Hit
GTACCAGGTCATGTCCATCCCTTGGTACATATATGCATTTGCTTTTAAACCATTTCTTTTGTTTAAATAAA100.1970831690973591No Hit
AGGAAATACTGATCACCTGTGCTTCTAAAGGCATTTGCTTTTCCTCTGGATTCCTCTACAAACTAGGCAAA100.1970831690973591No Hit
TAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTA100.1970831690973591No Hit
GTCCTGCTTAGTTTTTACTTGCTGGATGATACCATAATGTATCAAGGAGCGTCCATGGATACAAGATAAGA100.1970831690973591No Hit
GCAGACAATAAATACAATAATAACTATGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.17737485218762317No Hit
TCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATAAGTTAACTTCCAATTTACATACTCTGCTT90.17737485218762317No Hit
CCTTAGATAGGAATTTGGGCAAGGTAAAAGAATAAGAATTTAGTCCTTCATTGTATACCGGAATTTGTGAG90.17737485218762317No Hit
CCTCAATAAAGTTTGTGTTTATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.17737485218762317No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG90.17737485218762317No Hit
AGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAA90.17737485218762317No Hit
GTGTAATAAGACATAACGTTTTTCCTTTAAAAAAATTTAAGTGCGTGTGTAGAGTTAAGAAGCTGTTGTAC80.15766653527788727No Hit
CCTCATTCCTACCTACACTGGAAGGAGCGCTCTCACTGTGGAAGAGTTCTGCTAACAGAAGCTGGTCTGCA80.15766653527788727No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT80.15766653527788727No Hit
TTGCACCATTGCACTCCAGGCTGGGCAATAAGAGCAAAATTCCATCTCAAAAAAAAAAAAAAAAAAAAAAA80.15766653527788727No Hit
GTTGTCTCTGATGCAGCTTATACGAAATAATTGTTGTTCTGTTAACTGAATACCACTCTGTAATTGCAAAA80.15766653527788727No Hit
TATTGTGTTTATCAATACAAATGTGGAGTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13795821836815136No Hit
CTATAGTCCCACCTACTCAGAGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTG70.13795821836815136No Hit
GACCCTGTCTCAATAAAAACAAACAAAAAAACAACTTTCAGAACATTAGGAATAGAGGGCAACTTCAACTT70.13795821836815136No Hit
ATTAAGTTCTGCCTTTGTTTGGGTGTTTTGTTGATTTATGGAAGATTCAGCCCTTTGTTATATTAATATCA70.13795821836815136No Hit
GTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTTTTTTTTCCTTTTTTGTCTATGAAGTTGCTG70.13795821836815136No Hit
ATAAGTTGCTTTGTTATATTTTATTTGAATTGAACTACGCTAGGCCTAAATGCCAATAAAATATACTTTTC70.13795821836815136No Hit
TGATTACCCCGGCCATGCTAGAAGAGGAAGAACAGCTTGAAGCTGCTGGACTAGAGAGAGAGCGGAAGATG70.13795821836815136No Hit
TCCCTACACGACGCGGGCATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13795821836815136No Hit
ATTTAAATAAAGAATTTGTCATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13795821836815136No Hit
TATTTGGGGTATTTATCTTTCATTCGTTGTCTGTGTAACAAATAACTTCTGCCATATGGGTTGTCTGCACA70.13795821836815136No Hit
GTGTTATCAACATACAATGGCTTTATCAAGATGTAACCCTGTTGTAAGTCAAGGAACATCTATATGACCAA70.13795821836815136No Hit
CCTGAAAAGTAGGAAGCAGAAGAAAAAAGACAAGCTAGGAAACAAAAAGCTAAGGGCAAAATGTACAAACT70.13795821836815136No Hit
TCCCTACACGACGCGGGATGCCAAACCAATTTTGGGTCGAGTGCATTTAACGAGAACAAAACAAAAGGCTC70.13795821836815136No Hit
CACCATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGG60.11824990145841545No Hit
GATTCCAGGGTGTGTCTTTACCTGTTCACTTAAGAGCACATTTTACAATATGGGATAAGCTATTGGAAAGA60.11824990145841545No Hit
CATGTAGAAATGTTCTTTGTGTATTTTTTCTACAGAGGATTTTCTCTGATTTTATTTTCTTTGTTTCTGAC60.11824990145841545No Hit
CCTGTAATCCCAGCATTTTGGGAGGCTGAGGCAGGTGGATCACTTGAGGTCAGGAGTTCAAGACCACCCTG60.11824990145841545No Hit
CCTAAGAAATTTACTGTGAAAAAGCATTTTAAAAAGAAAAGGTTTTAGAATATGATCTATTTTATGCATAT60.11824990145841545No Hit
ATTATGTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11824990145841545No Hit
ATTTGAGTTCAAATATTTTTGAAATATAAAAATTGGTTGTATTTTTTAAAGCTATAATTCTTGTAGACATT60.11824990145841545No Hit
ATTCTGATTGTAATGGGTCCAAAAGAGGATGGAAGGGGAAGGATTGGAAGCAAAGCAGATTGATAACTCTT60.11824990145841545No Hit
CTTCCGATCTAGTTGGTTCGGGGACGGAAGTGTAGGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAAAAAA60.11824990145841545No Hit
GAGTAGGGCTTTTTTGTTTTGTTTTTTTTTAAAGGTGCTTGCTTGTTTAATGTAAATAATAGAAAGCCTTA60.11824990145841545No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC60.11824990145841545No Hit
GAGGGAAGTTGTTAGCAATAAATGTCTACCTTAAGAAAAAGAAAGATTTCAAATAAACAGCCTTACTTTAC60.11824990145841545No Hit
GGCCATTTAAGTTTAGTAGTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGG60.11824990145841545No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA60.11824990145841545No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGA60.11824990145841545No Hit
GGTTCGTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11824990145841545No Hit
ACATTAAACTGTTTGAAATCACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11824990145841545No Hit
CCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACA60.11824990145841545No Hit
TAACTATGCAGCTCATGCTGTGAAATCCAGGGTCCAGGAACACCCAGCCTCTGCTGAGTTTGTTTTAAAGG60.11824990145841545No Hit
TGATCATGTTTCCTGTGTTGTAGATTATGTAACTGCATGGCTTACATGAGGGGTCCTCATGTAAGTGCAGC60.11824990145841545No Hit
ACCCTTCACAGACTGCTCTGGTGCCTAGTGGTGCTAGCTCCTCCCACCTCAGCACCTGCTGCATCTGGAGC60.11824990145841545No Hit
CCTATAATCTCAACACTTTGGGAGGCTGAGGTCAGAGGATTGCTTGAGCCCAAGAGTATGAGACCAGCCTT60.11824990145841545No Hit
AATTAGGGTGTGGTGGCATGTGACTGTAGTCCCAGCTACTCGGGAGGCTGAGACCAGAGGATCACTTAAGC60.11824990145841545No Hit
CTGTAGGAAAGACCACATGTTAGGCCACAGAACAAGTCTTAACAAATTTAAGAAGACTGAAATCATAGTAA60.11824990145841545No Hit
GCTCTAAGCACTCATTTGGTAATAGCTTGTAAAAACACACCTGTAATCTCAGCCACTCAGGAAGCTGCGGC60.11824990145841545No Hit
AACATTTGGGATAGTCGTCTTTAAAAGACTTGGTGTTATTTACAGTGTTTGTTTTGATAACATTTGGCTGG60.11824990145841545No Hit
GATGGAGACCATCCTGGCTAACACAGTGAAACACAGTCTCTACTAAAAAATACAACAACAACAAAAAAAAT60.11824990145841545No Hit
CACGATTAGTAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11824990145841545No Hit
GACTAATACAGATGGTTTAAATAATTTCTCAGAAATGTTACTAAATCGTATCAGTCCATTTCAAGCCAATA60.11824990145841545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)