FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29596
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5911.9968914718205162No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2300.7771320448709285No Hit
CATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTCT930.3142316529260711No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG690.23313961346127857No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT610.20610893363968105No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA600.20273009866198133No Hit
GTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAA450.152047573996486No Hit
GTACAATTTGTTTCTATTAAGCAAGTACCAGTTTACCAATACATGAGTAACTGAAGTGTAACTGTTAAATG410.13853223408568724No Hit
GAAGAACAACAGTAATGATATTGTAAATGCGATTATGGAATTAACAATGTAACCATATGGAAGCAACTTTT390.13177456413028787No Hit
GATTTGAGGATATTCTTAATGATCCATCACAAAGCGAAGTCATGGGAGAGCCACACTTGATGGTGGAATAT360.12163805919718881No Hit
GCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTC360.12163805919718881No Hit
CATCCCTCTCCAGAAGAGGAGAAGAGGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTT350.11825922421948912No Hit
TTCCTACTTCATGGATGTGAAATGCCCAGGATGCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGG340.11488038924178942No Hit
CTTCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACCAACCTCCACAGGCTGGGTG330.11150155426408975No Hit
GTCTCATTGTGCCATGAGGTAAATGTAATGTTTCAGGCATTCTGCTTGCAAAAAAATCTATCATGTGCTTT330.11150155426408975No Hit
GTAGTAGGGACTCTGAAAGAGTTCATACTGTCTAACCTGTTAATTCCAATCACATTGAAAACAACTCATAT330.11150155426408975No Hit
CTTCCAATTTACATACTCTGCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGAAA330.11150155426408975No Hit
GGATATATACTTGAATGGAAATACATGTACTATTAGCAATCATATTTCATTTATCCCTGTAATTAGACAAG320.10812271928639006No Hit
GGTGGGACGACAGTGAAATCTAGAGTAAAACCAAGCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTT320.10812271928639006No Hit
GTTTTATCTTATCTACACATGGGGGACTGAAGAGTTCGAGAGATCCAAGAGGAAGAATCCAGCTGCCTATG320.10812271928639006No Hit
CCTTTTGGCCCTGCAGCATGTCATGCTCCCAGAATTTCAGCTTCAGCTTAACTGACAGACGTTAAAGCTTT310.10474388430869036No Hit
GTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAGCAGCACTA310.10474388430869036No Hit
CTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTCT300.10136504933099066No Hit
ATATAATTCTGGGTTTGTTTCTTGGGGAGTCAGTGAAACAAGTTTAAACATTTGTTAATAATTATTGAAGC300.10136504933099066No Hit
GAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGA300.10136504933099066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)