FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5075
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1783.5073891625615765No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1603.152709359605911No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG470.9261083743842364No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA360.7093596059113301No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT300.5911330049261084No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA200.3940886699507389No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC140.27586206896551724No Hit
CTTATAATCTTGTTTGCGGTGAGGACACTTAAATCTACTCTCTTAGTGATTTTCAAGTATACAGTATGTTG110.2167487684729064No Hit
TTGACGATGGAAATGGTCTCAAGGGAAACAGGAATGTCACTTTGACCCTGTCTTGGAACGTCGTACCAAAT90.17733990147783252No Hit
GCCCATCCCCCAAAAAACCATCCATCCCATCCTAGTGTCTGGTGGTGTCCGGTGGTGTCCATCTTCCATTC90.17733990147783252No Hit
GAACAAGTTGGGAGAATGGAAAGAGAACTTAACCATGAGAAAGAAAGATGTGACCAACTGCAAGCAGAACA90.17733990147783252No Hit
CATGTGCCATAATATACAAACTTCTACTTTCGTCAGTCCTTAACATCTACCTCTCTGAATTTTCATGAATT90.17733990147783252No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG90.17733990147783252No Hit
CCCAGAATATGGAGGCACCAAAGTAGTTCTAGATGACAAGGATTATTTCCTATTTAGAGATGGTGACATTC90.17733990147783252No Hit
ATAGAACTCCAACAACATCAATTTCATTGCACAGACTGACTGTAGTTAATTTTGTCACAGAATCTATGGAC80.15763546798029557No Hit
GATCAACGGGACAGAAAGTTAATAAGGATATCCAGAAATTGAACTCAGCTCTGCACCAAGCAGACCTAATA80.15763546798029557No Hit
GCTTAAAGTTGTGTAATACTAAAATCACGAGAACACCTAAACAACAACCAAAAATCTATTGTGGTATGCAC80.15763546798029557No Hit
GCTCTTTTCAGTTTTTGCTTATACACAATTCATTCTTTGCAGCTAATTAAGCCGAAGAAGCCTGGGAATCA80.15763546798029557No Hit
GGATTCAAATGGGGATTTGCTGCATTTGTGGTAGCTGTAGGAGCTGAATATTACCTGGAGTCCCTGAATAA80.15763546798029557No Hit
GCCAAAACTCCACAGTCAATATTAGTAATTTCTTGCTGGTTGAAACTTGTTTATTATGTACAAATAGATTC80.15763546798029557No Hit
GTGCTGCACCAGCAGGAGGTCCTGCCCCCTCCACTGCTGCTGCTCCAGCTGAGGAGAAGAAAGTGGAAGCA80.15763546798029557No Hit
GAATCAACTAAGTTTGTCTTACTGGAAGTAATATGAGAATTCAATACAGTGGCTATATAAAAATCAAGACA70.13793103448275862No Hit
GCCCCGAGTTGTTTCCTCGCTGATCGATTTCTTTCCTCCAGGTAGAGTTTTCTTTGCTTATGTTGAATTCC70.13793103448275862No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA70.13793103448275862No Hit
GGCCACCGATTGGCGGCTGATCCTGGACTGGGTACCTTACATCAATGGCAAGTTTAAGAAGGATAATTAAT70.13793103448275862No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAA70.13793103448275862No Hit
GCCCGGGGATTAACCAGCGTTATCAACCAGAAGCTAAAGGATGATGAGGTTGCTCAGCTCAAGAAAAGTGC70.13793103448275862No Hit
GAAGAAAAGAAAAAGGAAGAGAGGAGTTGATTATAATGCCGAAATCCCATTTGAAAAAAAGCCTGCCCTTG70.13793103448275862No Hit
GTGTTGATGCACATACAATGTATAAAGACATAATTTGTATAACAATAATAGCAAATAGGAGAAAGGAGGGA70.13793103448275862No Hit
CTCCCAACCTTTTGTAATGCCATTTTCTAAACTTATTTCTGAGTGTAGTCTCAGCTTAAAGTTGTGTAATA70.13793103448275862No Hit
GCAGGGCAATGGGCGTCTATAGGAGGTGGCTCTGCTCTGTTCTGGGGTTGGTCCAAAGTCAGGTGGAGTTC70.13793103448275862No Hit
AGTAAAATACGCCGGAGGGATACTCTTGCTATCAAACTTGGCAACAGACCATCTAAGAAAGAACTAGAGGA70.13793103448275862No Hit
CCTCAGCCTCCTAAAGTGCTGGGATTACAGCTGTGAGCCACCCTGCCCGGCCACTTTTGTATGATTTCTAA70.13793103448275862No Hit
TCCCTACACGACGCGGGGAACCTAGGAAGTATTTTAAATATTTATGAAAATATTTTGTTTTAAAATGAACT70.13793103448275862No Hit
GAAAAAGTATATGGGACCTTAAGCTCACCTTCTTTACTTGTATCAAATGATGACTGGTATACTGGTCTCCC70.13793103448275862No Hit
GATTTTATTGTCTTTATTTTTACCAAAGATTTCCCATAGTTTGAGCATTCAAAGCAATAAAATATAAAAAT60.11822660098522167No Hit
AGGAAGAATCCAGCTGCCTATGAAAATGACAAATGAGCAACGCATCCGGATGACGGTTCCCTGTCTCTGAA60.11822660098522167No Hit
GTTGTGGGACTGCATGCTACTGTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCC60.11822660098522167No Hit
ATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGCCAAGTGGGATGCCTGGAATGAGCTGAAAGGG60.11822660098522167No Hit
CTGTGCTGTTGAATTGCAAAGCTTTGTGGAACAGTTTTAGTAATATATCTGGAAAGGCTTCTGGCACTTAG60.11822660098522167No Hit
CCTGGAGCCAGTCCCACCACGCTCGCGTTTCCTCCTGTAGTGCTCACAGGTCCCAGCACCGATGGCATTCC60.11822660098522167No Hit
GTCTCTCACCACATACAAAAATCAACAAAAATCAACAAAAGGTGGATTAAAGACTTACATGTAAGACCTGA60.11822660098522167No Hit
GTAACAATTCAAACTGGAACCTCAGGACCCGAAGCAAGTGCAAAAAGGATGTGTTTATGCCGCCAAGTAGT60.11822660098522167No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC60.11822660098522167No Hit
ACACCCTGATTCGGCCTGATGGAGAGAAGAAGGCATATGTTCGACTGGCTCCTGATTACGATGCTTTGGAT60.11822660098522167No Hit
TCCTAGAGAAGCACAACTGTCAAGAGTCTTGTACACACAACTTCAGCTTTGCATCACGAGTCTTGTATTCC60.11822660098522167No Hit
CTAAAAATACAAAAACAAAATTAGCCGGGCGTGGTGGCAGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTG60.11822660098522167No Hit
GTAATGTTGATGGCCGCCATCCTCATGACATCATCGATGACATCAACAGTGGTGCTGTGGAATGCCCAGCC60.11822660098522167No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACC60.11822660098522167No Hit
ATAAGGAATTGCACATGAGATGGCACACATATTTATGCTGTCTGAAGGTCACGATCATGTTACCATATCAA60.11822660098522167No Hit
GGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAGAATAGATCTAGGTCCAAAGA60.11822660098522167No Hit
GTTCCCATGCCTGGAGAAGCTAATGCCAACTCATCATGTGATAATTCAATTTGTACAATAAATTATGAACC60.11822660098522167No Hit
GCCCAAGCCTCAGGATGGACAAGAGGAAACCCACAGCTCAGCTTCAGGCTTCTTATGTTTCTGAAAACAGC60.11822660098522167No Hit
CACTGAGGACTGTGAACATTCTTATATTATTTCATGTATATTGAAGAACATTGTTATGCAATGCTTTGTGT60.11822660098522167No Hit
GTAACTATTTCAACTGAGGAACAAGCACAACTTACTAGTACCCCTGCTATTCAGGATCAAAGTGTGGAAGT60.11822660098522167No Hit
GAATGCTGAAATGAGGCCTCTTGGTTGTTGTATGATTTTAATTGGTATAGATGAAGAGCAAGGCCCTCAGG60.11822660098522167No Hit
TGATCCTATACAGAAACTCTTTGTGGACAAGATTAGAGAATACAAATCTAAGCGACAGACATCTGGAGGAC60.11822660098522167No Hit
TTCTTTGCACTTAAAAATTAGCATGCTGCTTTTTGTACTTGGAAGTGTTTCAAAAAATTATATGACCATAT60.11822660098522167No Hit
GTAGGAAGCAGAAGAAAAAAGACAAGCTAGGAAACAAAAAGCTAAGGGCAAAATGTACAAACTTAGAAGAA60.11822660098522167No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGC60.11822660098522167No Hit
GCCTTCTGTACTTGAAGTTGATTTGAAATATGTAAGAATTGATGATGTATTTGCAAACATTAATGTGAAAT60.11822660098522167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)