FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7438
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAG3054.100564667921485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1361.8284485076633505No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA761.0217800484001076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400.5377789728421619No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT250.3361118580263512No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCC240.32266738370529713No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAA240.32266738370529713No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.30922290938424307No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC210.282333960742135No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT210.282333960742135No Hit
GCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGC210.282333960742135No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA200.26888948642108096No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT200.26888948642108096No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAGCACCCCCTCTACCCC190.2554450121000269No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC190.2554450121000269No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAGCA140.18822264049475665No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC140.18822264049475665No Hit
CTTACAAGGTTTTCAACAAAAGTGAAGTTTGCTAAAAGTTAACAGTGTAACATGTATTATGGTAACTTCTA120.16133369185264856No Hit
GCTTTGGGAGTCTAAGGTGGGAGGATCACTTGAGGCCTGGAGTTTGAGACCAGCCTGAGCAACATCTCTCA120.16133369185264856No Hit
ACTCAATATAATGTTAATATTACTGTAGCACTTACTAGCATTTCTGAAGGTTGGTCTTGAGATAAGATTGA120.16133369185264856No Hit
GAACAGTGAATACTAGTGGAATTGTTTGGGCTGCTTTTAGTTTCTCTTAATCAAAATTACTAGATGATAGA120.16133369185264856No Hit
CCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC120.16133369185264856No Hit
GAACTAGAGAAACATGGTTATAAGATGGAGACTTCATAGCATCCAGAAGAAGTGTTGAAGTAACCTAAACT120.16133369185264856No Hit
CTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG110.14788921753159454No Hit
GTATAGGAGTGGGTGGGGTGTTGAGCCACCCATCAGACCCTCTTTAGCTGTGCAAGATAAAAGCAGCCTGG110.14788921753159454No Hit
ACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.14788921753159454No Hit
ACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAGGG110.14788921753159454No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.14788921753159454No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAGCACCCCCTCTACCC110.14788921753159454No Hit
CTCCTGCCTCCACCAGTTCAAACTCAAATTAAAGGCCATGCCTCTGCTCCATACTTTGGAAAGGAAGAACC110.14788921753159454No Hit
GTCGAGGGCAAGGACTCTGCGTGCATTCTCCACTGCCGTCTACTCGGCCACTCCGGTCCCGACACCTAGCC110.14788921753159454No Hit
GTATTACATCTTGACTTGTAATCAATTATGAATATTTCTTGATATTTAATGTATAGGACATTTATTTATAC110.14788921753159454No Hit
TGTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCC110.14788921753159454No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.14788921753159454No Hit
ACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTA110.14788921753159454No Hit
TGCACATACTCTAAAAAGTACTCATCCCTTGTTTTCTGCTCATTTCTTCCAGCCTGAGGAACAGACGTCAG100.13444474321054048No Hit
GTAATAAAAGTTTACAACTTGATTTTTTCAAAAAAGTCAACAAACTGCAAGCACCTGTTAATAAAGGTCTT100.13444474321054048No Hit
CACACAGAGAAGAGGTGAAAGCAGTGGATAGGCAAAATAAAGGCAAAGGTTGAAGTGATGCAGCCTCAAAC100.13444474321054048No Hit
GCTCTCCACCTCCAGTGTGGTTATGTCATTATTATTAGTGCCTCTGTTAACTTTTGTTATTTTAAATAATG100.13444474321054048No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.13444474321054048No Hit
ATCCAGGAGGCTGCGCGGCCCCGGCTCCTCAGCTCCCTCAGTGTGCCCCGTGGTGTCACCGGGACTCCAGG90.12100026888948642No Hit
GTGTGAGCCTGCGCAGGACAGTTTCAGGTGGTTTTAAAGAACACGTGGAAATCCCTTGAATTTAGGACCTG90.12100026888948642No Hit
GCTACATCTCCAAGATGTTCCTGCGCGGGGACTCAGTCATCGTGGTCCTGCGGAACCCGCTCATCGCCGGC90.12100026888948642No Hit
CACTTAAGAAAACCGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.12100026888948642No Hit
GTTTTAAGTTATTAGTTTTTAAAATCAGTACTTTTTAATGGAAACAACTTGACCAAAAATTTGTCACAGAA90.12100026888948642No Hit
GAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTT90.12100026888948642No Hit
CACCATTGCAGATGCTTAAGTCAACTATTTTAATAAATTGATGACCAGTTGCAAAAAAAAAAAAAAAAAAA90.12100026888948642No Hit
CATCTCATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.12100026888948642No Hit
GTCTTAATATTTTGTGTAATTAATACAGTATCTCCGAGTGAACATACGATTTGCATTAGAGAAAGAACACA90.12100026888948642No Hit
TTACTCCCCCTCTAAACACTGCTGCTGCCTTAATTTTAGAAAGCAGCTTACTAGTTTACCCTTGTGGTATA90.12100026888948642No Hit
TAATAAGACATAACGTTTTTCCTTTAAAAAAATTTAAGTGCGTGTGTAGAGTTAAGAAGCTGTTGTACATT90.12100026888948642No Hit
GTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCT90.12100026888948642No Hit
GTGTGATATTTTTCATTATTTTAGGACGCCAACATGAGACCTGTAATAAAATATGTAATGGGGTTGAAAGC90.12100026888948642No Hit
TTCCCTACACGACGCGGGGAGTGGTTGAAGTGGCAGCTAAAATTTGTAAGTCTGTATGTAATATCTGAAGC80.10755579456843238No Hit
GACCAGAGATCCTTGCTGCTGCAGTTCCTCCCTCTTCCCTAAAGAACAATAGGGAACAAGTAGAAGTGGTA80.10755579456843238No Hit
TTCCCTACACGACGCGGGATTTGCTCGCAGTATCCTAGAATCTTTGTGCTCTCGCTGCAGTTCCCTTTGGG80.10755579456843238No Hit
AAGCAAGCATCTAAAAAGACTGCAATGGCTGCTGCTAAGGCACCTACAAAGGCAGCACCTAAGCAAAAGAT80.10755579456843238No Hit
TTTTAATGGAAACAACTTGACCAAAAATTTGTCACAGAATTTTGAGACCCATTAAAAAAGTTAAATGAGAA80.10755579456843238No Hit
CTGCCCTGGCTCTCCTAGAAGGTGGAGGACAGACACAGGAGAAATAAAAAAAGATGATGCTGCAGGAATCC80.10755579456843238No Hit
TTAAAAAACTTACTACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10755579456843238No Hit
GGACATGACACCAGTGGCATATCACGGCCATGGGGTCTCAGCATTCCGCTGCTGCTCGCCCCTCCTCCTGC80.10755579456843238No Hit
CCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTCCCCCTCAAACCTAAAAA80.10755579456843238No Hit
ATCCATTACAGAATCCCATGCAGTGATTCCAGGATTTGAAATTGTATGATGTGTTACATAAGAATTTATTT80.10755579456843238No Hit
GAAAGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAA80.10755579456843238No Hit
GAGTGGAAGTGCCATGTGTCACTTCTAGGCAAAACTTAGGAGCCAGTGCTAATATATATAATAATGGAATA80.10755579456843238No Hit
ATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGT80.10755579456843238No Hit
TGAGGAGGGGTACCGCAAGCTCATCGACCAGAAGAAGGACAAGCGCCTGGCCTACCTCTTGCAGCAGACAG80.10755579456843238No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA80.10755579456843238No Hit
ATCCAGGTGGGCTCTAAATCAAATGACAGGTATCTTTATGAAGGAGACACAGAAGAGACACACAGAGAAGA80.10755579456843238No Hit
CAATTAAGAGAGCTCAAATAATGGGGTTTATGCCAGTTACATACAAGGATCCTGCATATCTCAAGGACCCT80.10755579456843238No Hit
GGGCTACAATGCAGTATACTGGGTTGGATGAAAGCACTGGCATGGTGGTCAACAGCCCAACAAATAAGAAT80.10755579456843238No Hit
GGCCTGAGCATCCATCTTCAGCACAAGTTCTGCTCAGAAGGAAAGGTGTATTTGTCCATGTTGGAAGACAC80.10755579456843238No Hit
GGTAGCTGTAGGTCTTAGAAATGGCATCAAAGGTGGCCTTGGCGAAGTTGCCCAGGGTGGCAGTGCAGCGG80.10755579456843238No Hit
ACCCATTACAATCTCCAGCATTCCCCCTCAAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10755579456843238No Hit
CTTTTTATTAGATAACGCTTAAAGGAAGTCAGTACATAAAACTGCCAAAAAAAAAAAAAAAAAAAAAAAAA80.10755579456843238No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAAAAAAAAAAAA80.10755579456843238No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGATATGTCTCCATACC80.10755579456843238No Hit
GGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAAA80.10755579456843238No Hit
GTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCC80.10755579456843238No Hit
CTTTTGGACTTCGGCAGTCTGTCCAACCTTCAGGTCACTCAGCCTACAGTTGGGATGAATTTCAAAACGCC80.10755579456843238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)