FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002881914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002881914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28124.84526967285588No Hit
CCTTATTAATGAATGTCTTTGACTTAAATCTAACCAAAAACTGCAACATTATTCTTTGTACATTTTCATTA110.9725906277630416No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.8841732979664014No Hit
GTGTCAGACTACTATCCAAGGGCAGGCCAGAAATAAGCGGAAACAAGAGACCAAGAGCTTAAAGCAGAAGG80.707338638373121No Hit
CCTGTGAGACCCCCAATGCTGGAACAAGAGAGGACCCCACTGACACCCCAGCCACAGATAGTAGCAGGGCT70.618921308576481No Hit
GTGTAGGGAGATGCTTGCTGCAGCTCTTCGAACAGGGGATGACTACATTGCAATTGGAGCTGATGAGGAAG60.5305039787798408No Hit
GAGGTTGGGAGTTCAAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCAACTAAAAATACAAAAATTAG60.5305039787798408No Hit
CTGCTGCAGCAGCGGCACTACAAGCCAAATCAGATGAGAAGGCGGCGGTTGCAGGCAAGAAGCCTGTGGTA60.5305039787798408No Hit
TCCCTACACGACGCGGGAGGATGATCAGGTCACAGTGTGTACGTAAGAGTCAGCTAATTATGGATATTGTC60.5305039787798408No Hit
GTCACCTCTTCTGCAATCATGACCTCTTGATGTCTCCATGGTGACCTCCTTGGGGGTCACTGACCCTGCTT60.5305039787798408No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG50.4420866489832007No Hit
AAATTAGGTCATGTACATTTTCATATTAGACTTTTTGTTAAATAAACTTTTGTAATAGTCATAAAAAAAAA50.4420866489832007No Hit
GATCTCAAACTCCTGACCTCGTGATGCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACATGCGTGAGCC50.4420866489832007No Hit
GTGTGCATGTTTGTTTTTATGTTCCTTTAGAAAACATTGACTGTTACCATTGAATGAGATGGATCAGTGGA50.4420866489832007No Hit
GAACCTGTAGAAGATGACCTCGTTCCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAA40.3536693191865605No Hit
GATAGAAAAAATGAGCCAGACATGGTGGGGCATGTCTGCAATCCCAGCTACTCGGGAAGCTGAGGCAAGAG40.3536693191865605No Hit
TCCCTACACGACGCGGGCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTCGATGCTCTTAGCTGAG40.3536693191865605No Hit
GCCTACCCCTCCTGCGCTGCCCCAGAGCCTGGGAAGGAGGCCGCTATGCAGGGTAGCACTGGGAACAGGAG40.3536693191865605No Hit
GGGTTTTCTGTGCTCAGACTGTAGCCTGTAGCTCTTGGCCTGTGTAGAGCCCCCTCCTGTGCCCTCAGTGG40.3536693191865605No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATAAA40.3536693191865605No Hit
TTCGTAGGATGGGTGAGTCAGATGATTCCATTCTCCGATTGGCCAAGGCCGATGGCATCGTCTCAAAGAAC40.3536693191865605No Hit
ATAATAAATGGGTTAATTTATGTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3536693191865605No Hit
ACGCGGGAGTTTGAGCAAGTCTGGAGAATGTGCGCGCACATATGGAGGGGGAGCTCCCCGGCCCTCGCTGC40.3536693191865605No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC40.3536693191865605No Hit
GAACATTGTCTACAAATAGTTGAGTGTGTTGAGTGGGGTACTGAACAGGTTAACAAGATAGACACGTGTTG40.3536693191865605No Hit
GGCACATACCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCGGAGG30.2652519893899204No Hit
ATATCCAAAGACAACCATCATTCCCCCTAAATAAATTAAAAAAACTATTAAACCCATATAACCTCCCCCAA30.2652519893899204No Hit
TCCCTACACGACGCGGGAGACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2652519893899204No Hit
CATCTCTTGAAGCCAAACTCCACCTTCTGTGCTTTTTGCTTGGGATAATGGAGTTTTTCTTTAGAAACAGT30.2652519893899204No Hit
GATATCTATTTATATTTAAAGTTTATGTTTCATGAACTGCAGCTGCAGGATTCTGGCATTTTGCATGCCAT30.2652519893899204No Hit
GTTTAGAGGCGGATTTGGTCGTGGACGTGGTCAGCCACCTCAGTAAAATTGGAGAGGATTCTTTTGCATTG30.2652519893899204No Hit
GGGATCCAGTGTATAAATCCAATATCATGTCTTGTGCATAATTCTTCCAAAGGATCTTATTTTGTGAACTA30.2652519893899204No Hit
GCGGAAGTACGCGGTCAAGGCCTGAGGGGCGCATTGTCAATAAAGCACAGCTGGCTGAAAAAAAAAAAAAA30.2652519893899204No Hit
CTTGGTAAATACGGCATCATCTGCATGGAGGATTTGATTCATGAGATCTATACTGTTGGAAAACGCTTCAA30.2652519893899204No Hit
CTCAAGTAGAGAAGGAGGACTTCAGTGACATGGTGGCTGAGCACGCTGCCAAACAGAAGAAAAAAAAAAAA30.2652519893899204No Hit
GTAATCCTAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAATCCAGGAGGTGGAGGGTGGAGGTTGT30.2652519893899204No Hit
TCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCCTCTTGCTTTCTTTTCTACCTTCAAAAC30.2652519893899204No Hit
GTGTACTTGGCTAAATGTAAGACATCTGGCATCATTTGCAGCACTGTAACACCTTCAGTCTCAGTTGTGCA30.2652519893899204No Hit
CTCCTGATTAATACAATGGAAGTTTCTGGGCATTTACAATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2652519893899204No Hit
GTGGTGGTGTGCACCTGTAGTCTTAGCTACTCAGGAAGCTGATGCAGGAGGGTGTCTTGGGCCAAGGAGTT30.2652519893899204No Hit
TTCCTAAGCTGTCCTTTTGAGGCTTAGTCAGTTTATTGGGAAAATGTTTAGGATTATTCCTTGCTATTAGT30.2652519893899204No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT30.2652519893899204No Hit
GTCTGGAGTACTGCCTGCGTTGCAGTGAAAGTAATGTTGTGAGGTTGATGAAAAGTAAACGACTGCATTAT30.2652519893899204No Hit
GTACATAGCTGTTTGAAATGCCAGAATGACTTCTGACATTCCAAGTTTTTCACAAAATATATTTTATCTGT30.2652519893899204No Hit
GCCAGGCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTGAAAGTTCATATC30.2652519893899204No Hit
TTTGTTTAACACATACCCGTGCCTGCACAAGCCGAATAATAATGACATCGATATGAATTTACTGCAAAAAA30.2652519893899204No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA20.17683465959328026No Hit
TAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACACCTATCCCCCATTCTCCTCC20.17683465959328026No Hit
CCCTATCAGGAGTTCACTGACCACCTCGTCAAGACCCACACCAGAGTCTCCGTGCAGCGGACTCAGGCTCC20.17683465959328026No Hit
TCCCTACACGACGCGGGGTCATCTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
GGTCCGCAAGGCAAGGGGGAAGAAAAGGGTCCCCTAGTTGAGGATAGTCAGGAGCGTCAATGTGCTTGTAC20.17683465959328026No Hit
GGAGCTTAGTGTTCGCTGTGCTCATGAAGAGGATCGCCTTGAAAGACTTCAAGCTCAACTGGAAGAAAGCA20.17683465959328026No Hit
GTCTGCAGCGGGTCCCTTTTGTGCTTCCTTCCCCTCAGGTAGCCTCTCTCCCCCTGGGCCACTCCCGGGGG20.17683465959328026No Hit
TTCCCTACACGACGCGGGGGTTGAAGCTCATGTCAAGAAAGTACAAGAAGCAGCACGAGCCTCTGGGAAAG20.17683465959328026No Hit
GTGCACAGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
GACCGAGATGAATCCTCACCATATGCTGCTATGTTGGCTGCCCAGGATGTGGCCCAGAGGTGCAAGGAGCT20.17683465959328026No Hit
ACGCGGGGACGAGAAAGATTCTGTTTTGTATATAGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
GTTTGTGAAGAAACATTAAAACAAGTTAAATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
GAGTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTTCAGTCATGGCATTCGCAGTGC20.17683465959328026No Hit
GGTGGAAGAAGTGGGGTGGGACGACAGTGAAATCTAGAGTAAAACCAAGCTGGCCCAAGGTGTCCTGCAGG20.17683465959328026No Hit
GGGAGGCAGAGCTTGCAGTGAGGCGAGATCCCGCCACTGCACTCCAGCCTGGGCGACAGGGCCAGACTCCG20.17683465959328026No Hit
CCAATATGGTGAAACCCCATCTCTACTAATATAACAAAAATTAGCCAGGTGTGGTGGTGTGCACCTGTACT20.17683465959328026No Hit
GGATTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
GAATTCATGGACGACACGAGCCGATCCATCATCCGCAATGTAAAAGGCCCCGTGCGCGAGGGCGACGTGCT20.17683465959328026No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA20.17683465959328026No Hit
CTGGGAAATCAGAGGTACGTGATCTGTTTGTGGCTGAGAGACAGTTTGCAAAGGAACAACATACAGATGGG20.17683465959328026No Hit
GATTCCTTTCTCTCTCGTTTTTGACCCCCATTGGGTGTATCTTGTCTATGTACAGATATTTTGTAATATAT20.17683465959328026No Hit
AAGCAGTTATTAATGAAGTTTGTAGGCACAGACCGCTGAATTTGGGTCCCTTTGTGGTACGTGCTTTCCTT20.17683465959328026No Hit
GGATCACTGGAATGTGGGGATTCTGAAACAGAAATGAAACTGTCCTTTTGACAACTCTCTTATATAATAAA20.17683465959328026No Hit
GATCGGGTGTTACAGGTACAAGTAGGTATGTTGCAGAGGAAAATAAATATCAAACTGTATACTAAAAAAAA20.17683465959328026No Hit
CCTGTATTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
TCCCTACACGACGCGGGGGAAAAAAGAAGAAAAAAAAGAAAAAACATAGAAAAAGAGAAAAAAAAAAAAAA20.17683465959328026No Hit
AAACCATGCTGGGTTAATAAATTGCCTCATTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
ACTAAAGATTAACAGGTTATAGTTTAAATTTGTAATTAATTCTACCATCTTGCAATAAAGTGACAATTGAA20.17683465959328026No Hit
GAGGCATATGGGATTTATACAGATTCACTTGTAAATGTTGGATTGGGGATTTTTGTGTAAATTTTCTCAAA20.17683465959328026No Hit
CAAGCCTCTGTGTCGACTTCATGCGCAACTGCGTCCGCTGCTGAGGCCATTGCTCCATCTCACCTCTGCTC20.17683465959328026No Hit
GTGTAAGGAAAAATAAAAGTGCTGCCGTAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAA20.17683465959328026No Hit
GCATATTCTGATTTTATTAAAATAAAAAGTTGAACTGCACAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
GTGGGGCAACTGAATCTGCTTTGCAGCAAAGTCACCCTTACAAAGAAGCTAATATGGAAACCACATGTAAC20.17683465959328026No Hit
GAAAATAAATAGGGAAGATGGAGACACCCTCTGGGGGTCCTCTCTGAGTCAAATCCAGTGGTGGGTAATTG20.17683465959328026No Hit
GGTCGCATAAATTTGTCTGTGTACTCAAGCAATAAAATGATTGTTTAACTAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
GCTGCCAGGCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTGAAAGTTCAT20.17683465959328026No Hit
GTCCACAGGGAAAGCGAGTGGTTGGTAAAAATCCGTGAGGTCGGCAATATGTTGTTTTTCTGGAACTTACT20.17683465959328026No Hit
GGGTCTGGTGGCTCATGCCTGTAATTCCAGCACCTAAAAAAAAAATTATAGTATAATGGGCCAGGGGCAGT20.17683465959328026No Hit
GTCCTACACATTCAAGACAAAGCCTACAGCAAGAACTCTGAGTAAATAATGAAGTGAATGCAATGCACATT20.17683465959328026No Hit
GGGAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17683465959328026No Hit
AATTTATTGGGGTGACCTTCTTGGGGACTCAGGGGCTGGTCTGATGGAACTGTGTATTTATTTAAAACTCT20.17683465959328026No Hit
CATAACTACTTGAGAGGCTGAGGCAAGAGGATCACTTGAGCCCAGGAGTTTGAGGCTACAGTGAGCTATGA20.17683465959328026No Hit
GGTTGCGCCTGCCCTCGGGCCCTCACGGAGGCGGGGGTTCCAGGGCACGAGTTCGAGGCCAGCCTGGTCCA20.17683465959328026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)