FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086
Sequences flagged as poor quality0
Sequence length66
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26824.677716390423573No Hit
ATACTGGAGGGATTTCATTTTTAATTCATCTTTATGAAGATTTAGAACTCATTCCTTGTGTTTAAA161.4732965009208103No Hit
ATTATATACACTATACTGGAGGGATTTCATTTTTAATTCATCTTTATGAAGATTTAGAACTCATTC80.7366482504604052No Hit
GTGCACACCTGTGGTCCTAGCTACTTGGGAGGCTGGAATGGGAGGATGCACTTGAGCCAGGGAGGT80.7366482504604052No Hit
ACTTACAGTTTCCTTTAAAAAAAAAAAAAAGTAAACCTGGCAGCTATAGAATACACTATGTGCATT60.5524861878453038No Hit
CAGTGGCACTGTTAATATCCGCACAAGAGACAATAAGGTCCACGGGGAACGCACCATTTCTGAAAC60.5524861878453038No Hit
ATTTTATTGTTTATTTTGTGTTTAATGCACAGCTACTTCACACCTTAAACTTGCTTTGATTTGGTG60.5524861878453038No Hit
GATTCGAAATGCACAGTTAGCACAGTATAACTTCATTTTAGTTGTTGGTGAAAAAGAGAAAATCAG60.5524861878453038No Hit
CTGGGCAATGTAGTGAGTCCCCATCATTGCAAAAAGTAAAAAATTAGCTGGGCATCATGGTGCACA60.5524861878453038No Hit
GTTTAAAGGGAATGTTTAATTGAGAAATAAACATTTGTGTACAAAAAAAAAAAAAAAAAAAAAAAA60.5524861878453038No Hit
GTCATAGCTATATGTGTTTCACTTTACTTACATTTTAAAAAATCAATTATCAGCAAATCACAGTAC50.46040515653775327No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.46040515653775327No Hit
ACACTATACTGGAGGGATTTCATTTTTAATTCATCTTTATGAAGATTTAGAACTCATTCCTTGTGT50.46040515653775327No Hit
CTTTAATATTTATTAAAGGAAAGAAATTGCCTTATGAGATCATCCTCATGTTAAACCTTGGATTAA40.3683241252302026No Hit
GATGAGGAACCGGAAAGCCCGCCAGAAAAGAAAACATCTACAAGCCCCCCACCCGAGAAATCTGGG40.3683241252302026No Hit
GGGCAATGTAGTGAGTCCCCATCATTGCAAAAAGTAAAAAATTAGCTGGGCATCATGGTGCACACC40.3683241252302026No Hit
GTCCCCATCATTGCAAAAAGTAAAAAATTAGCTGGGCATCATGGTGCACACCTGTGGTCCTAGCTA40.3683241252302026No Hit
ATGCCAAATTCATGGCAGACATTGATCTGGATCCAGGCTGTACATTGAATAAAAAGATTCGAAATG40.3683241252302026No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3683241252302026No Hit
AGATTCCTCAAAATATTTTCTGTTAATAAATTGCCTTCATGTAAAAAAAAAAAAAAAAAAAAAAAA40.3683241252302026No Hit
CAGTAAATGCCAGTAGTGACCAAGAACACAGTGATTATATACACTATACTGGAGGGATTTCATTTT30.2762430939226519No Hit
GTACCCATGTAGACTAGCAAAATAGTATGTAGATGTGATCTCAGTTGTAAATAGAAAAATCTAATT30.2762430939226519No Hit
TATATACACTATACTGGAGGGATTTCATTTTTAATTCATCTTTATGAAGATTTAGAACTCATTCCT30.2762430939226519No Hit
GATTATATACACTATACTGGAGGGATTTCATTTTTAATTCATCTTTATGAAGATTTAGAACTCATT30.2762430939226519No Hit
TCCCTACACGACGCGGGCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGC30.2762430939226519No Hit
TCTTTATGAAGATTTAGAACTCATTCCTTGTGTTTAAAGGGAATGTTTAATTGAGAAATAAACATT30.2762430939226519No Hit
TTATAAGACCAAGCGAGTCCTCCCTCCCAATTGGAAATATGAATCATCTACAGCCTCTGCCCTGGT30.2762430939226519No Hit
TTCATATCCATCTATTTGTGGTTTCCATCTGATTTTTGACATAGATGATTATTGTTGTCTGTGAAT30.2762430939226519No Hit
TGATTATATACACTATACTGGAGGGATTTCATTTTTAATTCATCTTTATGAAGATTTAGAACTCAT30.2762430939226519No Hit
GGCTAAGGAGAAGCGCCAGGAACAAATTGCGAAGAGACGCAGACTTTCCTCTCTGCGAGCTTCTAC30.2762430939226519No Hit
GTAATAAAATTTTAAAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2762430939226519No Hit
GCCGAGTGTGGTGGCACATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTG30.2762430939226519No Hit
TGTTTAACCACTTTGCTGCTAAGATTTTGCCGTCCCATTCCCATTTTTTCCTTTACAATTTTAAAC30.2762430939226519No Hit
AGCTACTTGGGAGGCTGGAATGGGAGGATGCACTTGAGCCAGGGAGGTCGAGGCTGCAGTGAGCTG30.2762430939226519No Hit
ATCTTCTTCATGGCTGCCTTTGCCTTCTCCAACGGCTACCTCGCCAGCCTCTGCATGTGCTTCGGG30.2762430939226519No Hit
CAATAAAGCACAGGCCTTACCGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2762430939226519No Hit
TTTTAGTTGTTGGTGAAAAAGAGAAAATCAGTGGCACTGTTAATATCCGCACAAGAGACAATAAGG30.2762430939226519No Hit
GCTGAGATGGGAAAGGGCTCCTTCAAGTATGCCTGGGTCTTGGATAAACTGAAAGCTGAGCGTGAA30.2762430939226519No Hit
CTACTTGGGAGGCTGGAATGGGAGGATGCACTTGAGCCAGGGAGGTCGAGGCTGCAGTGAGCTGTG30.2762430939226519No Hit
CTATACTGGAGGGATTTCATTTTTAATTCATCTTTATGAAGATTTAGAACTCATTCCTTGTGTTTA30.2762430939226519No Hit
GTGTCAAGCCCATTCAGATAAGCTATAATCTGGTCTTTAAGGAACACAACTTTAAAACTGCAGCTT30.2762430939226519No Hit
CTCTTGCCATCACCTAGCTGCCTGCACCTGCCCTTCAGGGAGATGGGGGTCATTAAAGGAAACTGA20.1841620626151013No Hit
GTAGTGACCAAGAACACAGTGATTATATACACTATACTGGAGGGATTTCATTTTTAATTCATCTTT20.1841620626151013No Hit
CCTATACACACGCACACACGCACACACACACACGGTTTCCTGTGCTACTTCTGGCACTTAGTAATT20.1841620626151013No Hit
GCCCCACAGGACGGCATCAGAATTAATGTAACTACACTGAAAGATGATGGGGACATATCTAAACAG20.1841620626151013No Hit
GTGTTTAATGCACAGCTACTTCACACCTTAAACTTGCTTTGATTTGGTGATGTAAACTTTTAAACA20.1841620626151013No Hit
CACCTGGGCCATGTTCCTGACTACCTGGTTCCTCCTGCTCTCCGTGGCCTGGTACGCCCTCACAAG20.1841620626151013No Hit
CCCTAAGAGAATGTCAATCATATAATGATAATTTGTGAAAGCTTTGAGAATCAATCATCAGTAAGT20.1841620626151013No Hit
GATTTAATTATTGTTGAATTAACAATTAAAATGAATCCCTTTGGAGGGATGGCATTGAATAACTTA20.1841620626151013No Hit
CCTTTAAATGGGTCAGAAGTTGTCTTTGACATTTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1841620626151013No Hit
CCCATGTACTAATAAAAGAAAGTCTTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1841620626151013No Hit
GGTTCGCGCGATCGGATGAGCTGACCCGCCACTACCGGAAGCACACAGGCGCCAAGCCCTTCCAGT20.1841620626151013No Hit
GGTGCACTCCAGCCTGGGCAATGCAGCAAGTCTCTGTCATGAAATGGATTGAAATTGAATGGGAAT20.1841620626151013No Hit
GCAGTAAGATAATATAAATTTTGTTGAATGTATTAACATCATATGGTCTGAAAATGTGGGTTTTTA20.1841620626151013No Hit
GCTTTGTGGTTGTGTACTCTTTTTCTTTGTTTTTTTAATAGATGGCATTGGCCGGGCATGGTGGCT20.1841620626151013No Hit
CAATTGGCCATTTAAGTTTAGTAGTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCA20.1841620626151013No Hit
TGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCGGAGGTTGCA20.1841620626151013No Hit
CTATAAACCCTAATTTTTTTTATTTAAGTACATTTTGCTTTTTAAAGTTGATTTTTTTCTATTGTT20.1841620626151013No Hit
CTTAGAAAGGAGCAGAGTAACAGATATGTTTACAGATCAAGAAAAGCAACTTATGGAAACAACTAC20.1841620626151013No Hit
GTGTATCATCGAGAAAGGAGAAGAACACTGTGGACATCTAATTGAGGCCCACAAGGAATGCATGAG20.1841620626151013No Hit
CAGTGATTATATACACTATACTGGAGGGATTTCATTTTTAATTCATCTTTATGAAGATTTAGAACT20.1841620626151013No Hit
TTGTTACTCCACGTGTCCTGCAGCACAAACGGCGGCGTATTGCTCTGAAGAAGCAGCGTACCAAGA20.1841620626151013No Hit
CCTCTGGATGAGAAGAGCCTTGCTTTATTACTCAATTATCTTCACGATTTCCTAAAGTACCTGGCA20.1841620626151013No Hit
GTTTAATGCTTGCAATCTGAGCTCTTGAACAAATAAAATTAACTATTGTAGTGTGAAAAAAAAAAA20.1841620626151013No Hit
ATATTATAGTGATAATTTTGTATTTTCACAAAAAAAAAGTTAAACTCTTCTTTTCTTTTTATTATA20.1841620626151013No Hit
CTGCTGTGTGAATAAATAATTCCTGAAGAATGAAGAAGATTAATTTTGGGAGTTCTTTGACGAACT20.1841620626151013No Hit
ACTTCACACCTTAAACTTGCTTTGATTTGGTGATGTAAACTTTTAAACATTGCAGATCAGTGTAGA20.1841620626151013No Hit
GAATAATGAAATGCGTGTGGTGCACTCCAGCCTGGGCAATGCAGCAAGTCTCTGTCATGAAATGGA20.1841620626151013No Hit
GTATTTCAAATGCAAGAAATGAAGAAATAAATCTTTGGCTCACAAAAAAAAAAAAAAAAAAAAAAA20.1841620626151013No Hit
AGGCTGCAGTGAGCTGTGATTGCACTACTGTGTTTCATCCTGGGTGACAAAGTGAGACCCTGTCTC20.1841620626151013No Hit
GTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1841620626151013No Hit
GGGTTGTAAAAGAAAAGAAAGCAGAATGTTTTATCATGGTTTTTGCTTCAGCGGCTTTAGGACAAA20.1841620626151013No Hit
GTGAATAAATAATTCCTGAAGAATGAAGAAGATTAATTTTGGGAGTTCTTTGACGAACTTTGATAT20.1841620626151013No Hit
CCTGTGGTCCTAGCTACTTGGGAGGCTGGAATGGGAGGATGCACTTGAGCCAGGGAGGTCGAGGCT20.1841620626151013No Hit
ACCTATCTCCCCTTTTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1841620626151013No Hit
GTTACTGTCATTCCCATTCCTTTTCGTTTAGAATCAGAATAAAGTTGTATTTCAAATATCCAAAAA20.1841620626151013No Hit
TGATAAATGTGTACAGGGTCCTTTTGCAATAAAACTGGTTATGACTTGATCCAAGTGTTTAACAAT20.1841620626151013No Hit
GTAAAAAATTAGCTGGGCATCATGGTGCACACCTGTGGTCCTAGCTACTTGGGAGGCTGGAATGGG20.1841620626151013No Hit
GTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGA20.1841620626151013No Hit
TAGTGAGTCCCCATCATTGCAAAAAGTAAAAAATTAGCTGGGCATCATGGTGCACACCTGTGGTCC20.1841620626151013No Hit
GATGTAGCCCTGAGGTAGTTCATGAAAATGCTGTGCACTCATTCCATGGAATAAATGTTGGAAAGC20.1841620626151013No Hit
TTTTTCTTCTCTCTATAATAAATCATTTCTTATCTTAACATTTTAAAAAAAAAAAAAAAAAAAAAA20.1841620626151013No Hit
AACATGGAGAAGCCCCGTCTCTACTAAAAATACAAAATATATATATATCAATATATATTGATATAT20.1841620626151013No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAAC20.1841620626151013No Hit
CCATTGCACTCTAGCCTAAACAACAAAACAAAACTCTATCTCAAAATAAAAAAAAAAAAAAAAAAA20.1841620626151013No Hit
GTAATGATGTTGAAATGGAAACTGAAGCTAATTTGGATACAAGAAAAAGGTCTCACAAGAATCTTT20.1841620626151013No Hit
GCTATACCATTAGGCAATCAAATATATGAAGGGAATGTCTCCTTATAAATTATTTCATCTAACAAA20.1841620626151013No Hit
TCATTGCAAAAAGTAAAAAATTAGCTGGGCATCATGGTGCACACCTGTGGTCCTAGCTACTTGGGA20.1841620626151013No Hit
GTTAATATCCGCACAAGAGACAATAAGGTCCACGGGGAACGCACCATTTCTGAAACTATCGAGCGG20.1841620626151013No Hit
TTCCTTGTGTTTAAAGGGAATGTTTAATTGAGAAATAAACATTTGTGTACAAAAAAAAAAAAAAAA20.1841620626151013No Hit
GTTCCTGGAGAAGCTAGAGCCTGATTGTAGGCTACTACTCATCAATTAACTTCTACAGTGGAGACT20.1841620626151013No Hit
GTATTTCAAATGCAAGAAATGAAGAAATAAATCTTTAACTCACAAAAAAAAAAAAAAAAAAAAAAA20.1841620626151013No Hit
TCCCTACACGACGCGGGAAGAACAACTGATTCAAAATAAGGTACAGATTTATTTGTGAATATTTTG20.1841620626151013No Hit
GGGCATGGATACGTCAAATCCCTCCCCAGAGAAATCTTATAAATGTTAGAGACGCATCAGAAGTGA20.1841620626151013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)