FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688
Sequences flagged as poor quality0
Sequence length66
%GC20

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72242.77251184834123No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.5924170616113744No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC90.533175355450237No Hit
AGACCAGCCTAGCCAACATGGCGAAACCCTGTCTCTACTAAAAATACAAAATTAGCCAGGCATGGT50.2962085308056872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC50.2962085308056872No Hit
TTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAGGGTTCGAGGGCACGAG50.2962085308056872No Hit
TGCTCGGGACGCTGAGGTGGGAGGATTGCTTGAGCCCAGGAGTTTGACACCAGCATGGGCAATATG40.23696682464454977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.23696682464454977No Hit
GTCAATGATGTGCCTTCTCCATATGAAGTCAGAGGTTTTCCTACCATATACTTCTCTCCAGCCAAC30.17772511848341233No Hit
GTCTACTGTTCTATCAATTGATTTCAAACCTTCAGAAATAGAAGTTGGAGTAGTGACAGTTGAAAA30.17772511848341233No Hit
GGTCCCAGGTGTGACTTCTCAGACTCTCAAACCTCAAGGCTATATAATAGGCAAACAGAGTTGATA30.17772511848341233No Hit
GGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACAGAATCGCCGGACAGGTGGCAGCTGCC30.17772511848341233No Hit
ATTTGTAGGTAGTGAGACAAAATTGATGATCCATTAAGTAAACAATAAAAGTGTCCATTGAAAAAA30.17772511848341233No Hit
CTGTGCGTGCTGACCATGGTGAGCCCATTGGCAGGGGTACCAAAGTGATCCTCCATCTTAAAGAAG30.17772511848341233No Hit
CCTGGGGGGTGGAGGTTGCAGTGATCCAAGATTGCGGCTCTTCACTCCAGACTGGGTGAAAGAACG30.17772511848341233No Hit
CACAGAAATTGTCAGCCAAGGAGGACAGTATTCATATTTTGAATGAGGAATATGAAACCAAATTTA30.17772511848341233No Hit
GCCCAGACCCTGTGCCCATGGAGCCCAGTGTGGTTGAGGTCATCTGCGAGCGCGGCTAGGCGCAGC30.17772511848341233No Hit
GCATGACCTTTCTGAGTACGATGCTGAGTATTCCACGTGGAACAGAGATTATCAGCTGTCTGAAGG30.17772511848341233No Hit
GATGAATTTTATAATCGTTACATCACCAAGGGAAATCTTTTTGAGCCAGTTATAAATGCACTTCTG30.17772511848341233No Hit
GATTTTTGGTAGTCCATTGAAGAAGGGAGTTTGAAAGTTGTTGTATACTGTTAACGATTGTCTGCC30.17772511848341233No Hit
CGCTCTTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17772511848341233No Hit
GACTGGGTCTATGAATAAGAGGTGGACACAACAGCAGTCTCCTTCAGCACGGCGTGGTGTGTCCCT30.17772511848341233No Hit
CCACAAGATCCTCATCGACAGATATGATGGACGTGGTCACCTGCATGACCTTTCTGAGTACGATGC20.11848341232227488No Hit
GAAAAAGTGGCGGAAAAGCTAGAAGCTCTCTCGGTGAAGGAGGAGACCAAGGAGGATGCTGAGGAG20.11848341232227488No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAA20.11848341232227488No Hit
GCACTCCAGCCTGAGCAATATAGCAAGAGCCTATCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11848341232227488No Hit
GCTGCCATTCTTCTGTAACAATGTTATCAGTAATGCTTTAAACTCCAGCACCTGGTTATGCATTTG20.11848341232227488No Hit
CCCTTGGCCGCCAGTTCCGTTCTCCTCACGGGCCGAACGGAACAAGGGGTCCAGCTTGCGGGGGAC20.11848341232227488No Hit
GCTCAAGACCAGCCTGGCCAACATGATGAAACCCTGTCTCTACTAAAAATACAAAAATTCAAAAAT20.11848341232227488No Hit
GTTTCATGCCCATCATCCTAAAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCC20.11848341232227488No Hit
CCCTACACGACGCGGGCTTCCGGTCTTTCTGGTCTCGGCCGCAGAAGCGAGATGACGAAGGGAACG20.11848341232227488No Hit
GATAATATACAAAGCAGTGTACCTAACAACCAAGCAATACACAGAAAACAACAGAAACGAGAGATT20.11848341232227488No Hit
CATTAATTCAATTAAAACCATTACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11848341232227488No Hit
ATGCTAACCATGGTTGCCAACTACATCTGTCCCTTCCCATCAATCCCAGCCCATGTACTAATAAAA20.11848341232227488No Hit
GTCTGAAATTGAAGATGAGACTTTTAATCCTTCAGAAGATGACTATGAAGAGGAAGAGGAGGACAG20.11848341232227488No Hit
CATGTGGGAAGTGCTACTTTTACCTTCTGATTAGTAAGACATTTGTGGAAGCAGGAAAAAACAATT20.11848341232227488No Hit
CATTTGAATGCATTTTGTTTGGTATTGTATTTATTCAATAAAGTATTTAATTAGTGCTAAGTGTGA20.11848341232227488No Hit
CTTTGGGAGGCCGAGGCAAGCGGATCATTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACGTGG20.11848341232227488No Hit
GAGGAGAAGAGGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTG20.11848341232227488No Hit
AATAAAGTCTCTTTTTCACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11848341232227488No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11848341232227488No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11848341232227488No Hit
AAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11848341232227488No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGC20.11848341232227488No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11848341232227488No Hit
GCCGAAATCGCACCACTGCACTCCAGCCTGGGTGACAGAGGGAGACTCTGTCTCAAAAAAAAAAAA20.11848341232227488No Hit
CTCCAGAAGCATGAAGACTGGCCTGAAGCCTTACTGTTTTATAGAGCCAGGCACTGTTGCTATTTG20.11848341232227488No Hit
CTTTTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11848341232227488No Hit
GATCATGCTGTTGCACTGCAGCCTGAGAGACAAGAACGAAACTCTGTCTCAAAAAAAAAAAAAAAA20.11848341232227488No Hit
ATATACAAGTATTTGCTTTTATCAAAATAATTCCAATAAAGCATTTTAAAGTAAAAAAAAAAAAAA20.11848341232227488No Hit
GTCGGAAGCTGGCATATCGGCCGCCCAAGTGACCCTGTCCAGTGTCTGCTTGCCATCCTGCCAGAA20.11848341232227488No Hit
ACGCTCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11848341232227488No Hit
GTGCATACCAGTTATGGACCCATAACAGTTTATTTTCTCAATAAAGAAGATGAAGGTGCCATGTAT20.11848341232227488No Hit

[OK]Adapter Content

Adapter graph