FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19782
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5612.8359114346375494No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1410.7127691841067638No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG430.21736932564958042No Hit
TAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAA400.20220402386007483No Hit
CCAAGGCAGTGCATGCATGTTGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAAACATCCACTTAAGT400.20220402386007483No Hit
GGTCATGTTCTTAGAGCTTTTTCTGACATGTTACTTTTATATATTCTTCTTATGAAGTCAAATTGTAAAAA390.19714892326357294No Hit
GACATAGACCTCACCAGTGCTTCATATACCATGATATAATCTGAAAGGGGCAGATTAAAAAAAAAAAAAAA340.1718734202810636No Hit
TGCCAATACTTCTACATTTGAGAAGGGTTTTTTTAGAAATACATTTAGTAAAGTCCCCAAGACATTAGTCT340.1718734202810636No Hit
TCCCTACACGACGCGGGGTGGCACTGGGTGTGTTACTGCGATTACACTGATGATTCTGAGCTGTGCTTCTT310.156708118491558No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA300.1516530178950561No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAG280.14154281670205238No Hit
GCTTGTCAAGCTATAACCACAAAAATAATGAATTGATGAGAAATACAATGAAGAGGCAATGTCCATCTCAA280.14154281670205238No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAAC280.14154281670205238No Hit
ATTGAAAGGCATTTTAGTGTGGTTTATGTGTAATATTAAAGATTATTCAACACCTCTCACATCTTACAGAT280.14154281670205238No Hit
CGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAA270.13648771610555052No Hit
CGTGTGTAGCCTTTTTTAATATCCTTAATGAGTTAAATGACTATGCAGGACAGCGAGAAGTTGTAGCAGAA270.13648771610555052No Hit
GGCCAGAAGAATTGCTTGGGCTCAGGAGTTCGAGCCCATCCTGGGCAGCATGGGGAGACTCTGTCTCTATA260.1314326155090486No Hit
GAACAATACTGTAGACCAAATGGACCTAACTGACGTTTCATCCAACAGCAACTGTATACACATCCTTCTCA260.1314326155090486No Hit
CTATCTATCTGGCTGGCAGGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTG260.1314326155090486No Hit
TCAATGGTGAGCCCACCAGTGAGGATTACTGATGTGGACAGTTGATGGGGTTTGTTTCTGTATATTTATTT260.1314326155090486No Hit
GTGAAATCTAGAGTAAAACCAAGCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCC250.12637751491254676No Hit
TCTACAGACAGTTCAAGGAAATTGCTGCAGTTATTGAAACGGTGTGAAGACGGATTCTTTGGTTGATAAAT250.12637751491254676No Hit
CAATGTAACCATATGGAAGCAACTTTTTTTGGTGTCTCAAAGGAGTAACTGCAGCTTGGTTTGAAATTTGT250.12637751491254676No Hit
GCTGTGCTCCTCCCGCCTGTCCCTCTTCCTGGCGCTCTCTTGTCCTTTAGTCTTTGTTTTGCTTCTTAGTG250.12637751491254676No Hit
ATTTAGAAATAACCCTCCCCCGACTATTGTTTCTGTTACCTATTTACTTTGATAAGGTTTTTTAAAGTTTA250.12637751491254676No Hit
ATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTA250.12637751491254676No Hit
CATAGTAATTAATTAAGAAAATCGAGCACCCTTAATTACCTAGAATGCAGGGTTGTTAGTTTTTTGGTTGA250.12637751491254676No Hit
GATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAG240.12132241431604489No Hit
CTCTTAATCAGTGGTGGAAGAACGGTCTCAGAACTGTTTGTTTCAATTGGCCATTTAAGTTTAGTAGTAAA240.12132241431604489No Hit
GCTACTCAGGAGGCTGAGGCAGGAGGATCAGCTGAACCCAGGAGGTCAAGGCTGCTGTGAGCTGTGTTCGT240.12132241431604489No Hit
GAGATTACAGGCTTGAGCCACTGCACCTGGCCTGAATTACTGACTCTTACTGAGAATAATCATGGAAAAAC240.12132241431604489No Hit
CAGATTACACCTTCCCACTTGCTGAAAAGGTCAAGGCCTTCTTGGCTGATCCATCTGCCTTTGTGGCTGCT240.12132241431604489No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGATT240.12132241431604489No Hit
GTTCCAATCATGAAAAGGGGGGATGATTTTGTAAAAGTGGCATTTCCTGGTCAGTGGTGGTCTTCAAGACG220.11121221312304114No Hit
ATAGAAGTCAAAGGGATGTGGTGAGTATATGGATGAAGGAATTTTATTTTTTTGGCCAAATGTAACTTCTA220.11121221312304114No Hit
AGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTA220.11121221312304114No Hit
CCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGT220.11121221312304114No Hit
CAAAAGCAAGGTTGGAAAATGTTTTATCTTTCTATAGAAAGTTGGGTACAGTATGTAACTGCGGGAAACCC220.11121221312304114No Hit
ATTTGAGGCCAAATTCTTCAAATATTTTTTCTGTTTCTTCTCTTCTGTCTTCTCGTTTGGAGACCACAAAC220.11121221312304114No Hit
GCATTGATTGTCTTATATTTCAAGTTAAGGTCCAAAAAAACTCCAGCTGTGAAAGCAACATAAATGGATTT220.11121221312304114No Hit
ACATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTC210.10615711252653928No Hit
CATGATGATGATGTGATTGATATTGAGGATGACCTTGACGATGTCATTGAAGAGGTAGAAGACTCAAAACC210.10615711252653928No Hit
AAATTATATCACCTGTGTTCTGAACTAAAATGATGGGAGTTCATTTTGTATTTACTTTGCAGTAAGTATCA210.10615711252653928No Hit
GTTACATTCATGAGAATGCTCTTTTCGGAATGATGTTTACATGCCCTTTTACTTTTCAGGTTTATAAAGTC210.10615711252653928No Hit
ATTTTAGATTTTGGATATTGCAATTATAGATGCTCAGCTAGTAAGTATAATGCAAATATTCCAAAATCCAA210.10615711252653928No Hit
TCACTACAGTGGCAATAATATTGAACTGGGCACAGCATGCGGAAAATACTACAGAGTGTGCACACTGGCTA210.10615711252653928No Hit
AATTGGGAGTGGTAGGATGAAACAATTTTGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGT210.10615711252653928No Hit
CTATAAAGGAGGATTTGAACAGAAAATGAGTAGGCGAGAAGCTGGTCTTATTTTAGGTGTAAGCCCATCTG210.10615711252653928No Hit
GCCTGAGGATAGAGGGTTCCTGAAGGAGCTGTTTGGGTAAAGAGAGGATTCCATAATTTTGATGATATCCG210.10615711252653928No Hit
ATCTACGTCTGATTGGTGTACCTGAAAGTGACGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGAT210.10615711252653928No Hit
TACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAA200.10110201193003741No Hit
GTTGGAAGGTGACAATAAACTGGTGACAACTTTCAAAAACATCAAGTCTGTGACCGAACTCAACGGCGACA200.10110201193003741No Hit
GTCTGGAGAGCGAGACCATCCTGGCCAACATGGCGAAACCCCGTCTCTACTAAAAACACAAAAATTGGCTG200.10110201193003741No Hit
CATATAATCAGTATCCTAATGGGTCAGCCAATGGCTTTGGTGCAGTTAGAAACTTTAGCCCCACTAACTAT200.10110201193003741No Hit
GTGTGAGCCTGGGCATTCCCAGAGAGGAAGGGCCGCTGTGCACTGCCCGGCCATCAGAAAGACAGAATTTC200.10110201193003741No Hit
ATTCAAGAGGATTTGGAGAAATTGGAACCTTCATACATTGCTGGTGAGAATCTAAAATGGTACAGCTGGCC200.10110201193003741No Hit
ACCTTGACTACCCTGGCCTGGCCCGTGGTGGCAGTCCAGGGCTATCTGTACTGTTTACAGAATTACTTTGT200.10110201193003741No Hit
CTACTAAAAATACAAAAAATTAGCCGGGCGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCTGGAGGCTGA200.10110201193003741No Hit
GTGCTTGCTCAGGGGCTTGTGGACATGGCTGTTCCTGCCAAAGCTGTAGCAGTTATCCAAAAGTTCTTTCA200.10110201193003741No Hit
GAAAGAGTCTCCCTTCACACAGACCATTATTTACAGATGCATGGAAAACAAAGTCTCCAAGAAAACACTTC200.10110201193003741No Hit
CGACGCGGGAGAAGAAAAAAGACAAGCTAGGAAACAAAAAGCTAAGGGCAAAATGTACAAACTTAGAAGAA200.10110201193003741No Hit
ATTAAAATTAGAACCTGAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.10110201193003741No Hit
GTATTGCTCTGAAGAAGCAGCGTACCAAGAAAAATAAAGAAGAGGCTGCAGAATATGCTAAACTTTTGGCC200.10110201193003741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)