FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16400
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4142.524390243902439No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1380.8414634146341464No Hit
CATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTCT460.28048780487804875No Hit
GGTTTTTGTATATTTATTGATGGATCAATAATAATGAGGAAAGCATGATATGTATATTGCTGAGTTGAAAG320.1951219512195122No Hit
GTGGTGTGATCTTAATATATTTGAAAAAAACTTCATTCTCGTGAGTCATTTAAATGTGTACAATGTACACA300.1829268292682927No Hit
GTGTGTTAGAGTGTGGGGGGAAAATTAGTCTTATTTTTCCCTACATGGGATACAACACTGTGAATTCAATC280.17073170731707318No Hit
TGGATTATTATTTCACCTCAAAAGGGAAGGGAATTTTGACGCAAGCTACAACATGGATGAAACTTGAGGAC280.17073170731707318No Hit
TACTCCACGTGTCCTGCAGCACAAACGGCGGCGTATTGCTCTGAAGAAGCAGCGTACCAAGAAAAATAAAG270.16463414634146342No Hit
GAGTCCTGTTTGCTAGAATCCTACTTTATCTTTTAAGTTTTGCTAATTACCCTGCAATGAATGTTGAACAT270.16463414634146342No Hit
GTGTTGTACTCGTAAAACAAGGATCATCGATGTTGTCTACAATGCATCTAATAACGAGCTGGTTCGTACCA270.16463414634146342No Hit
CTTTTCTGTGGATTCATCATCTATGACACACACTCACTGATGCATAAACTGTCACCTGAAGAGTACGTATT260.15853658536585366No Hit
TCCTAAGACAAATTACTCTGAATGATCTTCCTGTGGGTAGATCAGTGGATGAGACACTACGTTTGGTTCAA260.15853658536585366No Hit
CATAAGGATGTTTCAGTTCCTCCATTTAACAGATATGAAGAGCATTTTAAGAGGTGCAACCTCTGGAAGTG250.1524390243902439No Hit
GCTCACCCTTTTGGAGTCAGAGCGAGAAGCCCGGAGGTTGCGCTGAGCTTGGCTGCTCGCTGGGTCTTGGA240.14634146341463414No Hit
GCACATGTTGAATCACTGTAGCTTTCGTAGGACATCTGATTATAATGCTTTTCTTTGTTTCTTTGTCCATA240.14634146341463414No Hit
CTATTAAACATGCTGTACATACTTTTTGGGTTTATTTGGAAAGGAATGCAAAGATGAAGGTCTGTTTTGTG240.14634146341463414No Hit
GAATATGCTAAACTTTTGGCCAAGAGAATGAAGGAGGCTAAGGAGAAGTGCCAGGAACAAATTGCGAAGAG240.14634146341463414No Hit
GATGTGAAATGCCCAGGATGCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGT240.14634146341463414No Hit
GTTCGTGCCAAATTCCGAAGCAATCTTCCTGCTAAGGCCATTGGACACAGAATCCGAGTGATGCTGTACCC240.14634146341463414No Hit
GGCCTGTCTGATGAAGAGATAGTAAAATTTTCTGAAGCAGAACATTGGCTTGATTATTTCCCGCCACTGGC240.14634146341463414No Hit
GCCTGCAGATACGTAATGTGACCACTGTTTTGTGTTGACAATATTGCTTTATACAGTTCTTGTATTTGAAA230.14024390243902438No Hit
GTTATTTCCAGAGGTGCGCTTTTTATGTACTTTTAGCTAGATGTGGCATGCATCTGTGAGCTTTAGATCAT230.14024390243902438No Hit
AGTTAACTTCCAATTTACATACTCTGCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTAT230.14024390243902438No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGA230.14024390243902438No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTC230.14024390243902438No Hit
AAGTTTCCCGTTAAAGGGAAGTGCTTTGAAGATGTGTACCCATTTTTGTAAGTTAATCATGATTATCCTGG230.14024390243902438No Hit
ATACCATGCTGTGTCTGTTATGCGGCAAAGCCGAAGATACTATCAGTATTCTCCCTGATGACCCCCGACAA230.14024390243902438No Hit
GGGCTTTGGAGGACTTGGGACAGCAGGGCCAATTTTTTTGCCCAAGTGCCTAGGCTGCTAACTCACTGACT230.14024390243902438No Hit
CATCTTAGTGGCAAGAGCACATGTCTTGCATTGGTCTCCAGCAGATTTGAACTCATCCTACCTCTGTGCCC230.14024390243902438No Hit
ATATAGCATAGTACCATACCACTTATAAAGTGGAAACTCTTGGACCAAGATTTGGATTAATTTGTTTTTGA230.14024390243902438No Hit
AGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTA220.13414634146341464No Hit
GTGATTGGGTGGGATTTTTTCCCTTTTTATGTGGGATATAGTAGTTACTTGTGACAAGAATAATTTTGGAA220.13414634146341464No Hit
CCCTTCCAGCGTCTGGTGCGAGAAATTGCTCAGGACTTTAAAACAGATCTGCGCTTCCAGAGCGCAGCTAT220.13414634146341464No Hit
GATGATAAGCATGGCCTGCCTTTATGGAGGTTCTGGATGTTCCTGTGGATAACCTCAAGGAGCACGGGACC220.13414634146341464No Hit
GCGTGTACGAAGTGAGGAATTCCTTTCCACCAAGAGCGCTCATTTTAGCGACAATACAGAATTCCCCTTCC220.13414634146341464No Hit
ACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTTTTTTTTCCTTTT220.13414634146341464No Hit
GCATAAGGTTTTTGGCAGTTTTGGATCAATAAATTTTTACTTTTAACTAAAAAAAAAAAAAAAAAAAAAAA210.12804878048780488No Hit
ACAATGCATCTAATAACGAGCTGGTTCGTACCAAGACCCTGGTGAAGAATTACATCGTGCTCATCGACAGC210.12804878048780488No Hit
GACTTGGACCGGAACAAGGACCAGGAGGTGAACTTCCAGGAGTATGTCACCTTCCTGGGGGCCTTGGCTTT210.12804878048780488No Hit
TGCATGATCCCTCATCCCTGAAATTGAGTTTATGTAGTCATTTTACTTATTTTATTCATTAGCTAACTTTG210.12804878048780488No Hit
GTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAAGAGAATTAATGTGCGTATTGAGCACATTAAGCA210.12804878048780488No Hit
GGAAGATGAAGAAGATGAAGATGAAGAAGAAGATGATGATGATGAATAAGTTGGTTCTAGCGCAGTTTTTT210.12804878048780488No Hit
GCGCTTGAGGACTTGGAGGTCCTGTCTAGGGAACTTATAGAAATGCTGGCAATTTCAAGAAACCAAAAGTT210.12804878048780488No Hit
GTATCTGGGACTGGTTTGGGCAGAATATTAAACTTATTTTGTTGCCAATTATTGTTTACCGAGTATAATGT210.12804878048780488No Hit
TCATGAAGGTGGAGGAGATCGCGGCCAGCAAGTGCCGCCGGCCGGCTGTCAAGCAGTTCCACGACTCCAAG210.12804878048780488No Hit
ATTGTAGACATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGG210.12804878048780488No Hit
CCATTATGCAGAATCCACGCCAGTACAAGATCCCAGACTGGTTCTTGAACAGACAGAAGGATGTAAAGGAT200.12195121951219512No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA200.12195121951219512No Hit
GAGTAATTTGCAGTTTGTTTGATTTATACTTTTGTTTATCTACAACCCAATAACAGACATGAGGGATGGCC200.12195121951219512No Hit
GGCCAACATAGCAAAACCCCATCTCTACTAAAAATACAAAAATTAGTCAAGAGTGGATGGTGCGTGCCTGT200.12195121951219512No Hit
TAATGGAAGAAGATGAAGATGCTTACAAGAAACAGTTCTCTCAATACATAAAGAACAGCGTAACTCCAGAC200.12195121951219512No Hit
GATCTACAATGAAGCCCTCAAGGGCTGAAAATAAATAGGGAAGATGGAGACACCCTCTGGGGGTCCTCTCT200.12195121951219512No Hit
CATACTCTTCTTCCTCCCCTGATTATTGCCATTAAGCCTTTAAATTCTAAACAAATTATAATGCATCATCT190.11585365853658536No Hit
GTACAAGCCCCTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAAGCACGAGGAGA190.11585365853658536No Hit
CACGTTAACTGCAGAGTAAATTGCTCTGTTCCCAGTAGTTATAAAGTCCTTGGACTGTTTTGAAAAGTTTC190.11585365853658536No Hit
GTCAAAAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGA190.11585365853658536No Hit
GAAGGGAGCTGTGGATGGAGGCTTGTCTATCCCTCACAGTACCAAACGATTCCCTGGTTATGATTCTGAAA190.11585365853658536No Hit
ATCCATACCTGTGCATGAGCTGTATTCTTCACAGCAACAGAGCTCAGTTAAATGCAACTGCAAGTAGGTTA190.11585365853658536No Hit
GAAATAAAAAAAATACTGATTTGGGGCAATGAGGAATATTTGACAATTAAGTTAATCTTCACGTTTTTGCA190.11585365853658536No Hit
AAATGGTCCCTGTTTGCAGATGACATGATTGTGTATTTAGAAAACCTCATTGTCTCCAGCCCAAAATCTCC190.11585365853658536No Hit
GATTACTGTCAATAAACAGCTTCATGCCTTTGTAAGTTATTTCTTGTTTGTTTGTTTGGGTATCCTGCCCA190.11585365853658536No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG190.11585365853658536No Hit
ACTCAGAGGAGGAGAAGGCAGCAGCCACCCCCAAGAAGACTGTACCTAAAAAGCAAGTTGTGGCCAAGGCC180.10975609756097562No Hit
GCTGCTGCTGCTGCTGCTGCTGCTGCTAAAGTTCCAGCAAAAAAGATCACCGCCGCGAGTAAAAAGGCTCC180.10975609756097562No Hit
AACTAAGCCTGCTGGTCAGGGCTGGCTTTCAGCCTTCCTAAGGCTCCTGGACTCCAGAGGCCAGCGGGGAG180.10975609756097562No Hit
GTAGCATGATCAGTGTATGCGAAAAGGTTTTTAGGAAGTATGGCAAAAATGTTGTATTGGCTATGATGGTG180.10975609756097562No Hit
GATGAGTGATTTAAGTGAGTCACAAGTCACAAAACTTTGCTATTCATAGTTAATCAAATAGAACTGGGTTT180.10975609756097562No Hit
AGTTTGCATATGTTTTCCTATGCAATAGTTGTTTTCCCAGTTATTCAAAGCAGCTTTCTATATGTAGAGAT180.10975609756097562No Hit
GCACGGGGCCTGGGTGACCGCCAATACTTGACCACCCCGTCGAGCCCCATCGGGCCGCTGCCCCCATGCTC180.10975609756097562No Hit
GTTTTTACTATCTAGCTGCCCAACGGGGCCTACTCACTTATTCTTTCACCCCCTCTCCTCCTGCTCAGCTG180.10975609756097562No Hit
CAAGCAAATCCCCATCTCGGATCACTGGAGCCAAAACCTGTCCCAGTTTTTCCCTGAGGCCATTTCTTTCA180.10975609756097562No Hit
CACTTAGTTAACAGCTAAGCACCCTAATCAACTGGCTTCAATCTACTTCTCCCGCCGCCGGGAAAAAAGGC180.10975609756097562No Hit
CCTCAATATGTGCCGCCAGTGTTTCCGTCAGTACGCGAAGGATATCGGTTTCATTAAGTTGGACTAAATGC180.10975609756097562No Hit
CCATGATGCAGCCATATGGAAGACTAGGATATGGGTCACACTTATCTGTGTTCCTATGGAAACTATTTGAA180.10975609756097562No Hit
GATTACAGGAGCACTGTGGAACGTCTGTAAATATGTTTTTATAATTCCATGTATAGTTGGTGTACACTCAA180.10975609756097562No Hit
GGACGTCAGAGAAACGGCGTCATGCCCAGCCACTTCAGCCGAGGCTCCAAGAGTGTGGCCCGCCGGGTCCT180.10975609756097562No Hit
CAACTGCAACAGAAAGATGAAGCTCTACAGGAAGAGAGAAAAGCTGCTGATAACAAAATTAAAAAACTAAA180.10975609756097562No Hit
GCTGGCTCTCTCTTCCTACCCTCCTTCCACATGTACATAAACTGTCACTCTAGGAAGAAGACAAATGACAG180.10975609756097562No Hit
ATACAGAGTGGTAGCTAGGGGTTGGTTATCGTCAGAAGGTGGTCTCCCCTCAGGCCTGGGGGATAAGGACG180.10975609756097562No Hit
ACAAAGCTGTCTGGGCCAAAGGAATAAGGAATGTGCCATACCGAATCCGTGTGCGGCTGTCCAGAAAACGT180.10975609756097562No Hit
CCATAGGGCCAGATGATGCTATAGACGCCTTGTCATCTGACTTCACCTGTGGGTCGCCTACAGCTGCTGGA180.10975609756097562No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTT170.10365853658536586No Hit
CCAGAAATTTGTCATTGCCACTTCAACCAAAATCGATATCAGCAATGTAAAAATCCCAAAACATCTTACTG170.10365853658536586No Hit
GTATGACCTTGCTGAGGGTATGTCCTGCTCCTTTCCACCAGTGATTTGTATTAAGACAGCACTTATATTGT170.10365853658536586No Hit
ATACTACAGAGTGTGCACACTGGCTATCATTGATCCAGGTGACTCTGACATCATTAGAAGCATGCCAGAAC170.10365853658536586No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG170.10365853658536586No Hit
TTTTAATTACTTTAGAGAGCTAGGGATGCAAATGTTTTCAGTTATTGAAATATGTTTTGTATAAATTTGTC170.10365853658536586No Hit
GATGAAGATTCACCAAATAAGCTATATACTTTGGTTACCTATGTACCTGTTACCACTTTCAAAAATCTACA170.10365853658536586No Hit
AACGAGTCCTGGCCTTGTCTGTGGAGACGGATTACACCTTCCCACTTGCTGAAAAGGTCAAGGCCTTCTTG170.10365853658536586No Hit
AGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAAC170.10365853658536586No Hit
GGGGCATGCGTGGGAAGGCCTGGAGAGCCCTGCAAAGACCGAAACAACCAACGTGGGAACCACATACAGAG170.10365853658536586No Hit
GGATGGAAAATACAGCCAGGTCCTAGCCAATGGTCTGGACAACAAGCTCCGTGAAGACCTGGAGCGACTGA170.10365853658536586No Hit
ACCATGGCCTATACATCCTTGGGAACCAGGTTCCTTTAGAGTTGGTCTTATTACCTTGAAGCTGGGCATGA170.10365853658536586No Hit
AAGCAGGAGTGACTTCAGTCATCCCAGCAATCGCTTGGTTCAGTTTCATTCAGCTCTCTATGGACCAGTAA170.10365853658536586No Hit
ATCTAACAGTAGCCACAAGTGCCAAGCAGACATTAGACATATCCTGAATAAGAATGGAGGAGTGATGGCTG170.10365853658536586No Hit
TATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCG170.10365853658536586No Hit
AGTCATTTCTGTTTACCTTTTTCTTCTGCCCAGAGTGTATTTGTGAAGAGTCTCTTATATTATGTTTTGTG170.10365853658536586No Hit
GTGCTTGACAGAGTCACCCCATGGTGGTATGGCTGAACAAGGAGCGGCAGACAACTCAGGGAGAAACTCAG170.10365853658536586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)