FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23148
Sequences flagged as poor quality0
Sequence length71
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5242.2636944876447207No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1080.46656298600311047No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1000.43200276481769484No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA950.41040262657681015No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT750.32400207361327116No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT550.23760152064973214No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC520.22464143770520134No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC480.20736132711249353No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC420.18144116122343182No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT370.1598410229825471No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.15120096768619318No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA340.14688094003801624No Hit
CTTTATTAGTGAGGAGAAAACAATTCCTTAGAAGTCTTAAATATATTGTACTCTTTAGATCTCCCATGTGT330.1425609123898393No Hit
GATTTACCCTACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTT320.13824088474166235No Hit
AATTTAGAGAGTTGAGAAGTAGGTATATTAAATTACAGAATCTTACTGAGTTTTGGTAGACTGATAATACA320.13824088474166235No Hit
GTACAGTAAACAATTAGCATGCTTCGGTGCCAGATTTTGATTTCTACTTTTAAAATACTAGCCTGTAAAAT300.12960082944530846No Hit
GTTCAATTTAACGTGGCAAAGGGCAACATTTAACATAATTCAACTGCTTTTACCTAAATACGCTTACTGCT280.12096077414895455No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC280.12096077414895455No Hit
GTATATGATTGCGAGTGGAAAAATAGGGGACAGAAATCAGGTATTGGCAGTTTTTCCATTTTCATTTGTGT270.11664074650077762No Hit
GTATATAAATTCCAAATAGAACCTGGCATCACCCTGAAGGAATTCTAACTTCACACTGTTGGGGAAATTTA260.11232071885260067No Hit
TTATTGTACTGTGTAAAGATGCATAGTCATCTCATTTGGTTGGCTTTGTACCTTGTACCTTTTTTAGCCTT250.10800069120442371No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA250.10800069120442371No Hit
GTGAAACTTAGTGTAACAATTTTGATCTGTTAAATTGGATTTTACATGTACATTTGAATGCCAGAATTTCT240.10368066355624676No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC240.10368066355624676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)