FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47421.496598639455783No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG210.9523809523809524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.6349206349206349No Hit
GAAAAAGAAAAATTTCAGTATCTGAGCCTTAGCGTATGTGTCACCAAAAGGATGATTCATAAAAGGAAAAA120.5442176870748299No Hit
CCAATTCCTACTTCATGGATGTGAAATGCCCAGGATGCTATAAAATCACCACGGTCTTTAGCCATGCACAA110.4988662131519274No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT110.4988662131519274No Hit
GCTATCTAGAGAGCCACATCCTGTTGACTGAAAGTGGCATGCAAGATAAATTTATTTGCTGTTCCTTGTCT110.4988662131519274No Hit
CTTTTGACTCTCCTCAAACTTAACTAGTAATAATCTACTGTTGGCCAGAAGCCTTACCAATAGTCAATTAA100.45351473922902497No Hit
AATCCATTGTGCCACTTTGGCATGTCAGCATTTATTTACGCAAAACATGACCTCAGGCTTACAGGCCTTTA100.45351473922902497No Hit
GGCGACACAGCGAGACTCCGTCTCAAATAAATAAATAAATAAAATAAAATAAAAGAAGAATAACAATAAAG100.45351473922902497No Hit
GTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCT90.40816326530612246No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA80.36281179138321995No Hit
GAGGCGCTGATAGCCTTGGCGGTGGCACTGGGGCCTGTGAGGGGTTAAAGGAGGCTGTTGCTGAACTTCCC80.36281179138321995No Hit
GCATCTGTGAACTGGCTGCTTCTTCCAGAGAGATCTCTTGGCAGAGTGAGGGCCTGGAGATAACCAGCTTT80.36281179138321995No Hit
ACTCCACACATGTATTTTTCTGACTCTGAAAGCAAACTACAGGCAACAGACAAAAGTGAAAACGAGCAAAT80.36281179138321995No Hit
GAAATTTCTTAGAGGCTTACATTTAAGGATCCCACTTACTGCAGTCACTCCTTGTGCTTTATTGCTTTTAC80.36281179138321995No Hit
CCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGTATGAAGATCGGGCGGATTGAGGATGTCACCCCCATC70.31746031746031744No Hit
GGTCTAGAAAACCAGCTTGCGAATAACAGTCCCCGTGGCCATCCCTGTGAGGGTGACGTTAGCATTACCCC70.31746031746031744No Hit
AAACAATCTCTAATATAGTGTTAAGTGAAATAAGCAATATGCAGAACTTAAAAATACTTTTTAGCCGGGCA70.31746031746031744No Hit
CAGCTGTACATATGTGGATAAGCTGATTAATGGTTTTGCAACTGTAATAGTAGCTGTATCGTTCTAATGCA70.31746031746031744No Hit
GGTTTTAATGATGATGTTCTACTTCAGATAGTTCACTTTCTACTGAATAGACCAAAAGAAGAAAAATCACA70.31746031746031744No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCT70.31746031746031744No Hit
GGCTTAAATCTTTAATAAAGGAAAACAAAACAATCCTCTTAAATTTCTTATAAATAGCTCTCCAGACATAT70.31746031746031744No Hit
CACAGAGCAAGACGAAGATGAAGAAATGGATGTGGGAACAGATGAAGAAGAAGAAACAGCAAAGGAATCTA70.31746031746031744No Hit
CCAACATGGAGAAACCCCATCTCTACTAAAAATACAAAATTAGCCGGGTGTGGTGGTGCATGCCTGTAATC60.27210884353741494No Hit
AGTAAATACAGAGAATTAATTATTCTAACATATTGGCCTCTTTGTACTGGAAAAGTATTCAGTGGTACCTG60.27210884353741494No Hit
GCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGAAG60.27210884353741494No Hit
GATTAGAGGGAAAGCATTTTCTATATCAATTGTGTTTAAACCTTTCAAGAAGGTTATTTAGCTAGCTTAGT60.27210884353741494No Hit
AAATTCGTGCTACACATATGTCTGATGGCCTTTACCTTTTCAAGAAAACAGTTGCAATTTTGAGCAAAATT60.27210884353741494No Hit
GCGCTCGTTATCATGTGGCAGAAGCACTATGTGTACGACCCCCTCCCGCAAAGCTTTGACAAAGAGTGGGT50.22675736961451248No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGGA50.22675736961451248No Hit
GACTGTCCTCGTCCTTGGCATTTTCACTGTTCTGTACAAGGCTGCTTGTTTTTTTTATTGCCAAAGTCAAA50.22675736961451248No Hit
GTGATCCTCCATCTTAAAGAAGATCAGACAGAGTACCTAGAAGAGAGGCGGGTCAAAGAAGTAGTGAAGAA50.22675736961451248No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.22675736961451248No Hit
GTCTTTCTATTGCACCGAGGTTGTTTTTTGTAATAGTATTTGTGGAAATGGAATTTGAGCCAGATGCTGTC50.22675736961451248No Hit
CTTTTCAACCAGCAGCAAGAAGTTCAAATTTTTTTCTGTCACTGTAACAGAAAACACAATATGTATATAAC50.22675736961451248No Hit
ACTTAAAGTCAGGAGTTCAAGACCAGCTTGGCCAACATGAGGAAACCACATCTCTACCCAAAAAAAATACA50.22675736961451248No Hit
GGTGGGCTGATGGCCTGGCTGCCTGGTGGTTGATGGTTTTGCTCCCCCTACCTTTTTTTTTGAGTTTATTC50.22675736961451248No Hit
CCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTAC40.18140589569160998No Hit
CCTACAATACAGCCTCTAACAAAACTAGGCTGTCCCGAACCCCTGGTAATAGAATTGTTTACCTTTATACC40.18140589569160998No Hit
AGTATATACATATATATGGGGGGAGGGGAAAGATTAAAAAATAGATTTACAGCCAGACATGGTGATAGCCC40.18140589569160998No Hit
GACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCA40.18140589569160998No Hit
GTGCGTATTGAGCACATTAAGCACTCTAAGAGCCGAGATAGCTTCCTGAAACGTGTGAAGGAAAATGATCA40.18140589569160998No Hit
GTACACAAATCAGTAGCTCTTCTATACACCAACAGCAACCAAGCAGAGAATGAAATCAAGAACTCAACATC40.18140589569160998No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA40.18140589569160998No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18140589569160998No Hit
CATGGTGAAATCCCATCTCAACAAAAATATGCAAAAATTATCTGGGTATGGTGACGCGTGCCTGTGGTCCC40.18140589569160998No Hit
GGGTAGCTGATGCCCTCCTGTATAGAGCCACCATGATTCTTACAGTTGGTGGAACAGCATATGCCATATAT40.18140589569160998No Hit
GTGTATTTCTCTAGTAATTGGTTTTGAATTTTATGTAATACCTAGATTTTACTGTTTATAAACAATCAAGA40.18140589569160998No Hit
GACTTCGAGGGGTTCGTGCTGTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGC40.18140589569160998No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACC40.18140589569160998No Hit
TATAATAATGGGTAATTTGTCAATAAAGCATTCCTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18140589569160998No Hit
CCTCTTCACACTAAAGGAAGAAAAACCTAAGATGTACTTCATGACCATGATCGTTTCCCTTGCTGCGGTTG40.18140589569160998No Hit
CCGTGGGCTGTTACTTGCTTTGAGTTGGAAGCGGTTTGCATTTACGCCTGTAAATGTATTCATTCTTAATT40.18140589569160998No Hit
GCTTTTTAGGCTTGTCACAAATGATTGCTTTGTTTTCCTAAGTCATCAAAATGTATATAAATTATCTAGAT40.18140589569160998No Hit
CTTTTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18140589569160998No Hit
ACATTATGACTTTTTTCTCTTAGTTTAAATAAACTCCAAGGTAACTGGACTTCTAAAAAAAAAAAAAAAAA40.18140589569160998No Hit
TCACTCAGGAGTTCAAGACCAGCCTGGGCAACATGGCGAAACCCTGTCTCTACGAAAAATACAAAAATTAC40.18140589569160998No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTGCC40.18140589569160998No Hit
GCATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
AAGTAACAGACTAGAACCTTCTTTCAAGTACCTGAATTGAAATGAAACTCATTTTGAATAATAAAAACTCT30.13605442176870747No Hit
TATTCAGGTTGTGTTGCTTTCTGCCACAATGCCAACTGTTGTGTTGGAAGTGACCAAAAAATTCATGAGAG30.13605442176870747No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
GGATATTAAAATGCTTGGGTGAGGTTTAGCCATCTTACTTGGCTTTTTACTATTAACATGATGTACTAAAG30.13605442176870747No Hit
GATAATAGTGGTTCTTGACTTTCACTTACCTCTCAGTGAACTGTGAGCCCTGCAGAAATATTGAAGTGTAA30.13605442176870747No Hit
CATATTGAGATGCCTGCCCTTGTTAGTATTCATTTTATGCTGCCCAAGATATCATTTAATTTAGACTTAAC30.13605442176870747No Hit
CCATTGAGAAGATGCAGGAATAAAGTAATCTTATATACAAGCTTTGATTAAAACTTGAAACAAAGAAAAAA30.13605442176870747No Hit
GTGCTCCATTTGTGATATAATTTCTAACATTTCTGCTCTGTGATGGGGGTTTATTTGTAAGAATAAGAGGC30.13605442176870747No Hit
ATATGTAGCTTTGTAAACAGTTTTATATCCTGCATCTTAGGGGGGAAAATCAGTTGACGCAGATTGAAAAC30.13605442176870747No Hit
TCTTGGTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
AATTAATAAAGATGACAAGTCAGTTGTCAATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
GTCAGGGGCGTATCCACAAAATCACCGAATTCATACAGATCGTTTAAATAAATGAACATCATTAAAGTCAA30.13605442176870747No Hit
GCACTATAGCCATTTGCCCCAACTTAAGTTTAGAAATTACAAGTTTCAGTAATAGCTGAACCTGTTCAAAA30.13605442176870747No Hit
GGTCTCTTTTAAATTAAAATATTTCTTTTCTCATGTGCAAATGTATATCAAAGACTTGAACAGGTATTCTC30.13605442176870747No Hit
GGGGCAGCCCCCAGTCCCTTTCTGTCCCAGCTCAGTTTTCCAAAAGACACTGACATGTAATTCTTCTCTAT30.13605442176870747No Hit
GAGCTGAGATCACGCCACTGCATTCCGGCCTGGACGACAGAGCGAGACTCTGTCAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
GCCATGTTGCTCACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
GCCCTGCTGCAAAGATGGTCAACGTACCTAAAACCCGAAGAACCTTCTGTAAGAAGTGTGGCAAGCATCAG30.13605442176870747No Hit
GCAGTGGACAAGAAGGCTGCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAAATG30.13605442176870747No Hit
GGTATGGGTTGGGAGCATAATAAATGTGTTTTGAGAATTGTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
GGAGGTGGAGGTTGCAGTGAGCCGAGATCGCGCCATTGCACTCCAGCCTGGGCAAGAAGAGTAAGACTCTG30.13605442176870747No Hit
ATAAAATAACATGCTGCCATTCTTGATTGCTAGAGTTTTTGTTAGTACTTTGGATGCAATTAAAACTATGT30.13605442176870747No Hit
GAATGAAGGAGGCTAAGGAGAAGCGCCAGGAACAAATTGCGAAGAGACGCAGACTTTCCTCTCTGCGAGCT30.13605442176870747No Hit
CTTTCTACTTGTGCATGGGCTTGGTTTATACGAATTGCCATTAAACATCGCTGCACCAAAAAAAAAAAAAA30.13605442176870747No Hit
CTGTTGAAATAAACCCAACTCTTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
GATTGCACCACTGCACTCCAGCTTGGGCGACAGATCAAAGCTTTGTCTCGAAAAAAAAAAAAAAAAAGGCA30.13605442176870747No Hit
ATATTTTTCTTCACAGCTTGAACTGAACACAATATTGCCCGGTTTAAAAAAAAAAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
GTATGCCAGCCTGCTTCTCACAAATAAAGTGTCTATACTGCCCTCAAGAACATGAATACTGGCCGGGCACA30.13605442176870747No Hit
ACCTGTCTGCCAGGTGGCTTCGAAAATGGAATTAATTTTTACATGGCATTGATAAGTTACTATTTCAATAC30.13605442176870747No Hit
AAATTAGCCAGGCATACTGGTACATGCCTGTAATCCCAGCTACTTGGGAAGCTGAGGTATGAGAACTGCTT30.13605442176870747No Hit
GGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGATGC30.13605442176870747No Hit
GGTCAAGCGCCTTGGTTTGCCCACTAGGATTGTTTTAAGAAAATGGCAGACAAACCAGACATGGGGGAAAT30.13605442176870747No Hit
CGACGCGGGCTTATCGAGGAGCAGAAAATCGTTGTGAAAGTGTTGAAGGCACAAGCACAGAGTCAGAAAGC30.13605442176870747No Hit
CAATGCTCAGCTTGAAGAATATTCCTGTTTTGGAGACTGCCTATAAGTTAATATTGGGAGAAATGACTTGT30.13605442176870747No Hit
ACTTCAATGGGGCCCAGTGCAAGGCTGTGTGTGTGGAGGCGGGCATGATCGCACTGCGCAGGGGTGCCACG30.13605442176870747No Hit
GTTTAACAGTTATAGTTTACTCAGCTTCACTGTTACATCCTAGATGAGTATTGTATTCAAAAATACTGGGC30.13605442176870747No Hit
AGCTGGAGCTCCCAAACTAAAAGACATTTGTTATTTTGGAAAGAAGAAAGACTCTATTCTCAAAGTTTCCT30.13605442176870747No Hit
GGTGGGCTGATGGCCTGGCTGCCTGGTGGTTGATGGTTTTGCTCCCCCTACCTTTTTTTTTGAGTTTATTA30.13605442176870747No Hit
ATTGTGTTCAACGGGAATATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13605442176870747No Hit
GCTCTAAGCTGCAGCAAGAGAAACTGTGTGTGAGGGGAAGAGGCCTGTTTCGCTGTCGGGTCTCTAGTTCT30.13605442176870747No Hit

[OK]Adapter Content

Adapter graph