FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23318.5214626391097No Hit
GACAAATGACTTGAAAGAAGTGGTCAATAAATTGATTCCAGACAGCATTGGAAAAGACATAGAAAAGGCTT141.1128775834658187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131.0333863275039745No Hit
GATATATCTTTTGATCTTTTATTATAAGGGTAGAATATAGATTAAGATCATCAAAATCCAATTTCACATGT131.0333863275039745No Hit
TTTAAGACATAGGCATGGGCAAAGATTTTATGATGAAATTGCCAAAAGCAATTGCAAAAAAAAGGAAAAAT131.0333863275039745No Hit
GGACTGGTCTCTAATGCTGTGGTCATTGCACAGAAGGGAAAGGTCTCAAGGAAGAGTCAACTGGGACAAGC110.8744038155802861No Hit
GGTGTACATCCTGCAGCTTATTGGCTTAAAATGTACTCTCCTTTTATGTGGTCTCTTTGGGGCCGATTGGG110.8744038155802861No Hit
GTCTAGAATCAGATAATGGGAACATTATGATCCAGAAACACGATGGCATCACGGTGGCAGTGCACAAAATG110.8744038155802861No Hit
GAATAATTGAACAATAAAGTTTGCTTTCAGATGCAGTTTTCAAATTATAATCTCATTTCAATTTATAACGT100.7949125596184419No Hit
GGTCTAGAAAACCAGCTTGCGAATAACAGTCCCCGTGGCCATCCCTGTGAGGGTGACGTTAGCATTACCCC100.7949125596184419No Hit
GCCTTCTGTACTTGAAGTTGATTTGAAATATGTAAGAATTGATGATGTATTTGCAAACATTAATGTGAAAT100.7949125596184419No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG90.7154213036565978No Hit
GGTGGAAGAACGGTCTCAGAACTGTTTGTTTCAATTGGCCATTTAAGTTTAGTAGTAAAAGACTGGTTAAT90.7154213036565978No Hit
CAATTGGAAAGCCTAGGTAGTTATTTGGGGAGGGGTCTCTACGCCTTGGCGAAGCGCACAGCTGCATCTTT90.7154213036565978No Hit
AATCCTATCATCAAGCTCACAAAGCTACTGGTGGCCCCTTAAAAAATATGGTTCTCTTCACTCGCCAGAGA90.7154213036565978No Hit
GCCTAGGAGGCCGACCCTCTTTGGAGTCCTGCTGTCTGGGTGCCAGGGCCGGAACGAGGTAGTGGCCATCT80.6359300476947536No Hit
ACCCCAAAACAATTACCATAGTAACATTAAATATCACTGATCAGAGACCACCATAATGATATAATAATGAT80.6359300476947536No Hit
CCTGGGACCGGGAAATCTAAGCTGGAAACATTGCCCAAAGAAGACCTCATCAAGTTTGCCAAGAAACAGAT80.6359300476947536No Hit
GTTTAGGACCAGCCTAGGCAACATAGCAAGACCTCATCTCAATTAAAATAAAAAAACCTAGGGCACTTAGG70.5564387917329093No Hit
GGAGGCAGAGGCTGCAGTGAGCCGAGATTGCACCACTGCACTCCAGCCTGGACAAGACAGAGCAAGACTCT70.5564387917329093No Hit
GATTTGAACAGGACTGATGAAATTATTTAGAAGTAGGCATACTATCATGGATGTATGGGATTATTTTCCAA60.47694753577106513No Hit
GGTACAGGAAGGGAACGTGGAAAGCGCATACAGGACCCTAAACAGAATCCTCACTATGGATGGGCTCATTG60.47694753577106513No Hit
GTTTATTAATGAGGTGACTTTCACCTTAGGACAACTGTTGCATGCCAAGTTTTTTGTGTGTGTGAAACACT60.47694753577106513No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT60.47694753577106513No Hit
CAGGTCTGTGGGCCTCAGGTCTGGCCAGCCAGGGCTGGTGCTGTCCCCGCCTACCTCCACTTCCTTTCCCT60.47694753577106513No Hit
CTTATATACAAGCTTTGATTAAAACTTGAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.39745627980922094No Hit
GTGGCCCACGGACTGTCAGCTATGCAGTGGAGTTTCACCCCGACACAGGCGACATCATCATGGAGTTCAGG50.39745627980922094No Hit
GCCTCATTTTCCTGTAGCTCCTCTGGTTCTGTTTGCTCATTGGCTGCTGTGTTCATCCAAGGGGGTTCTCC50.39745627980922094No Hit
GGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAATTAATACCAATAGAAG50.39745627980922094No Hit
ACAATCTTCCACTGGAGGCTTATGGTATTTAAGACATTTGTAAGGCACTGGGGAAACACAGGAGGATAAAA50.39745627980922094No Hit
CTGGTGGGATGAAGGTAAAGGCAGACCGAGATGAATCCTCACCATATGCTGCTATGTTGGCTGCCCAGGAT50.39745627980922094No Hit
GTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGA50.39745627980922094No Hit
GCTACTGTGGCAGTCATGGCTAGAACTCAAGAAAATGCATCAGAAAGAAACCAGTTCAGTGAAGAAGTTTC50.39745627980922094No Hit
CTGAACTGAGTGCCATCTCCATGCTGTACCTGGATGAGTATGATAAGGTGGTACTGAAAAATTATCAGGAG40.3179650238473768No Hit
CAGTTTAAGAAATCATTGGCCCCTTCCCAGCACATTGAATGGGTAAGCAGACAGGCCATGATTTAGTTGGC40.3179650238473768No Hit
ATAATAGCTATTTTATATATTGTAGTATCAACATTTTTAAATTAAATGTTTTACATTCAAAAAAAAAAAAA40.3179650238473768No Hit
ACACGACGCGGGGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGA40.3179650238473768No Hit
ACATGAAGAAACCCTGTGTCTACAAAAACTACAAGACAAATTAGTCAGGCTTGGTGGTGTATGCCTGTGGT40.3179650238473768No Hit
GGTTGAAACTATGTAAATGGTTTTTATGGAAATTATCAGTTACAATATTTTAAAGGTGTAGAATGGCATCT40.3179650238473768No Hit
CAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCCTTTTTAATATGCT40.3179650238473768No Hit
TAATAAAATTTTGTACAAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3179650238473768No Hit
GTCAAGGCCTGAGGGGCGCATTGTCAATAAAGCACAGCTGGCTGAGAAAAAAAAAAAAAAAAAAAAAAAAA40.3179650238473768No Hit
GCTTTTCATTAACCACATTCCTGCACAACTCATTTCTGAAAACCTACCATGTTTCTTTACAGAGCCATCCA40.3179650238473768No Hit
GATAAGACATAGATTTAGCATCCATTTGGGTTTATATTCTATTGCAATAAATATATTCTCATAGATTAGCA40.3179650238473768No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA30.23847376788553257No Hit
ATTATGACATGAGGTAAAAGAAACAAATTATGGCCAACCATCACTATGCCTGAAAATGACTGGTTTTCAAT30.23847376788553257No Hit
CAAACTGCTGCATGAGTGGCTGCCCCAACTGCGTGTGGGTGGAGTACGCGGACAGGCTGCTGCAGCACTTC30.23847376788553257No Hit
CCATTATTTATGATCCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.23847376788553257No Hit
TGCTTAAGTCAACTATTTTAATAAATTGATGACCAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.23847376788553257No Hit
GCCCTGGGGGTTGCAGGCTGCGGTGCCTCAGGCCCCTCCTTCAGCCTGTGGCCACCTCTGGGGCACGATGG30.23847376788553257No Hit
GGCTGGTCTCAAACTCCTGGGCTCAAGTGATCCTCCCACCTCGGCCTCCCAAAGTGCTGGAATTACAGGCA30.23847376788553257No Hit
GTTTATTATTGTTGGATACAAAGTTCTTCATTGATAAGAGTCCTATAAATAAGATAAATACGAAGATAAAG30.23847376788553257No Hit
GAATTAACTGAAACCCAGCCAGAAGAGGGACCACCTGTAAAGCAAGTCCTTTCAAGTTTCACTGCACATCC30.23847376788553257No Hit
GCCTCCTCCCCTACTAACTCCTTCTTGAACTTGATAGCTCCATTGTCACTTTCGAGTCTTCCCTAAACCAC30.23847376788553257No Hit
GGGCCTGGGCAGTCAGGCTCCAGGGCCCTATGCCAACCTGCCTTTGTTACAAGGCAGAGGAAGCTTTGTAT30.23847376788553257No Hit
GTCTCATATATTTGGAGTTTTTCTGAAAAATGGAGCAGTAATGCAGCATCAACCTATTAAAATACATTTTA20.1589825119236884No Hit
CTGGTTTGTCTGCATGTGTAAAGATTTTTACAAGGAAATAAAATACAAATCTTGTTTTTTCTAAAAAAAAA20.1589825119236884No Hit
GTATACACTGTACTAAGAAATTGTCATTCAATAAATACGTTTTGAGTGCTGTCTAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA20.1589825119236884No Hit
GCAAGTTTGAACCAGCTGCTGGTGTAATGTACAGTTATATTTGTCTATAAATGGAGCTGTTTATGGCAATT20.1589825119236884No Hit
ATTTAGATGTTAATTAAAGGAAACAAACAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
GTACAGGAAGTTGGAAATTTTTGGTTTATGACTTTGTCTAATAAAGAGATAGTTCTAAACACAAAAAAAAA20.1589825119236884No Hit
GAGCCGAGTCTGTGGGCACTCTCTGCCTTCATGCACCTGTCCTTTCTAACACGTCGCCTTCAACTGTAATC20.1589825119236884No Hit
GGTCTAGAAAACCAGCTTGCGAATAACAGTCCCCGTGGCCATCCCTGTGAGGGTGCCGTTAGCATTACCCC20.1589825119236884No Hit
GCCTAGGAGGCCGACCCTCTTTGGAGTCCTGCTGTCTGGGTGCCAGGGCCGGAACGAGGTAGTGGCCATCG20.1589825119236884No Hit
CTGTTACTTTTTCTAGTGCTGCTTTGTGCTGAGAGAACATTTTAGTTTACTGCACTTGACCTGTAAAAGAC20.1589825119236884No Hit
CCCTACACGACGCGGGGGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
ACTTCATTAAGGTTTCATTCAGTGTAGCAATTACTGTCTTTAAAAATTAAGTGGAAGAAGAATTACTTTAA20.1589825119236884No Hit
GTACCGAAATAGTCTTTACAAATATTTGTTTAGGTTCCCAGATCTGACTTTTATGTTTATCTTCATTCTCT20.1589825119236884No Hit
GGACTACAGGCACACAACACCATGCCCAGCTAATTTTTTAATTTTTTTGTAGAGACGGGGTTTCACCATGT20.1589825119236884No Hit
CTTCTTGCCAGGAGAAAGAAACTTGCTGGGACCACAGTGCAGGATGGGCACTCCGTGATGGCCCCAGCCAG20.1589825119236884No Hit
GGAAAAGGAACCTTTTTATTTTTTGCATCTCCATAGCATAGTTTTTGGCATATGAATGTTTAATAAATGTT20.1589825119236884No Hit
GATGAGATATTCTAGGAGAAATGGATAAATTCCTAGAAATAAACAACTGTCTAAGACTAATCATAAAGAAA20.1589825119236884No Hit
GGTTGAGATAGGAAGACAGGGAGGGTATTCTCAGCAAAGGAACTAGTGTAAGTACAGACACTAAGGCAAAA20.1589825119236884No Hit
GCACCTAATACAGAATAATTTCTTAAAAATGCATAGGCTGGCCAGGCGTCAGTGGCTCATGCCTGTAATCC20.1589825119236884No Hit
CCTCTCCACTGTCTCCACTCTCCAGCGCCCATTCAAGTCTCTGCTTTGAGTCAAGGGGCTTCACTGCCTGC20.1589825119236884No Hit
GTCTAAGAGATGTGATGTAACATAGTTTCTGTGAACTGATGTTACTTTCTTGTTTTTCAAATAAAGGATCA20.1589825119236884No Hit
GGGAGAGGCTGAGGACAAATACCTGCTGTCACTCCAGAGGACATTTTTTTTTAGCAATAAAATTGAGTGTC20.1589825119236884No Hit
GCTTGAACCTGGGAGGCGGAGGTTACAGTGAGCCGAGATTGTGCCACTGCACTCCAGCCTGGGTGACAGAG20.1589825119236884No Hit
GCCACATAATAATAAAATTCTTTTACCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
GTGACTGGAAGCAAACGCCCTAAAAGTTACCCATCACGTTTCAGTGTAAATGAGTAACTATAGAAGACATT20.1589825119236884No Hit
GCAATCCACAGAACTCTCTCTCCTTCCTTCCACCTGTCAGCTTCTCTGCTTCTGAGATAAGAACCATTTGT20.1589825119236884No Hit
GGCTTTGTGTTTGGAGGATAGGAGGGAGAGAGGGAAGGGGCTGAGGAAATCTCTGGGGTAAGAGTAAAGGC20.1589825119236884No Hit
GTGTCTAGACATATTTGCATAGAGGCCAAGGTATTCTATTCTAATAACTGCTTACTCAACACTACCACCTT20.1589825119236884No Hit
GGCATAAATGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
GCAAGAAGGCTCTCACTGGATGTGGTCCCTTGATCTTGGACTTCTCAGCCTCTGTAACTGTAAAAAATAAA20.1589825119236884No Hit
CTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAAAACAAAAAAAA20.1589825119236884No Hit
ATAATAAACTAAGTATGTGGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
TTTTTGTAAAGACCTTGCTGAATGTTTCCAATAGACTAAATACTGTTTAGGCCGCAGGAGAGTTTGGAATC20.1589825119236884No Hit
TTATTAAACTGTCCCCCAGATCGACACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
CCTTAAAACACCACCCTCTCATCACTAATCTCAGCCCTTGCCCTTGAAATAAACCTTAGCTGCCCCACAAA20.1589825119236884No Hit
AATAAATACCTAGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
GTGCTCGAGTCACATTCTGCTTAAAGTTGTAACAAATACAGATGAGTTAAAAGAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
TTGTTGTACGTACACATTGTGCTCTGGAGTGCCTATTTATTGAAATCATTGTAAGACCTGTTATAAATTTA20.1589825119236884No Hit
CTGGCCGCCTTTGTAACCTTCTCTAAACGTCAGGCCTGGACTTAAAAGATTTTTTAAAACCTCCATAAGTA20.1589825119236884No Hit
AGTATTCAGTGAATACTTGAGAAATGTACAAATCTTTCATCCATACCTGTGCATGAGCTGTATTCTTCACA20.1589825119236884No Hit
GAGTGTAAAGTAATCTTGGAATATAAAGAATTTCTTCAGGTTGAATTACCTAGAAGTTTGTCACTGACTTG20.1589825119236884No Hit
ATCCAGTGGTGGGTAATTGTACAATAAATTTTTTTTGGTCAAATTTAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
AGACTAAGTATGTTGGTTAATAGGAATCCTTTTTTTTTTTTTTTAAAGAATAAATGTGAAAAAATAATAAA20.1589825119236884No Hit
CCATGGACAGCCACTTAACTGGATTTCTTTGGAGCAAAATGGCATTATGGTAGAACTGCCACAGCTAACCA20.1589825119236884No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
ACTTCTGCCACCTACCAGCTATATGGCCTTGAATGAGTCATTCAGCTTTAATAAGGTTCATTTTCTTCTGT20.1589825119236884No Hit
AGTTAGAACTTGTTACTATTGTAAAGGAAGAGTCAACTGGAAAATTCAAGGAGTTAATAAAATTTGTTTAC20.1589825119236884No Hit
GAGATGAACCTCCTGTAACTTCTATCTGTTCTTTTTTGAGGCTCAGGGAGAAACTAGCATTTTTTTTTTTC20.1589825119236884No Hit
ACATAAATGGATTTTAAACTGTCTACGGTTCTTAACCTCATCTGTTAAGTTCCCATGCCTGGAGAAGCTAA20.1589825119236884No Hit
TCCCTACACGACGCGGGAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit
GGGAAGGTGGGTGGGTATTCTCCCCAATAAAGACTTAGAGCCCCTCTCAAAAAAAAAAAAAAAAAAAAAAA20.1589825119236884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)